Biotechnology for biofuels and bioproducts最新文献

{"title":"Recent advances in microbial ε-poly-L-lysine fermentation and its diverse applications","authors":"Shubo Li, Yunren Mao, Lifei Zhang, Miao Wang, Jinhao Meng, Xiaoling Liu, Yunxia Bai, Yuan Guo","doi":"10.1186/s13068-022-02166-2","DOIUrl":"https://doi.org/10.1186/s13068-022-02166-2","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43809026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Looking into the world's largest elephant population in search of ligninolytic microorganisms for biorefineries: a mini-review.","authors":"Bame Rammala,&nbsp;Nerve Zhou","doi":"10.1186/s13068-022-02159-1","DOIUrl":"10.1186/s13068-022-02159-1","url":null,"abstract":"<p><p>Gastrointestinal tracts (GIT) of herbivores are lignin-rich environments with the potential to find ligninolytic microorganisms. The occurrence of the microorganisms in herbivore GIT is a well-documented mutualistic relationship where the former benefits from the provision of nutrients and the latter benefits from the microorganism-assisted digestion of their recalcitrant lignin diets. Elephants are one of the largest herbivores that rely on the microbial anaerobic fermentation of their bulky recalcitrant low-quality forage lignocellulosic diet given their inability to break down major components of plant cells. Tapping the potential of these mutualistic associations in the biggest population of elephants in the whole world found in Botswana is attractive in the valorisation of the bulky recalcitrant lignin waste stream generated from the pulp and paper, biofuel, and agro-industries. Despite the massive potential as a feedstock for industrial fermentations, few microorganisms have been commercialised. This review focuses on the potential of microbiota from the gastrointestinal tract and excreta of the worlds' largest population of elephants of Botswana as a potential source of extremophilic ligninolytic microorganisms. The review further discusses the recalcitrance of lignin, achievements, limitations, and challenges with its biological depolymerisation. Methods of isolation of microorganisms from elephant dung and their improvement as industrial strains are further highlighted.</p>","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41470033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptome and proteome analyses of Trichoderma longibrachiatum LC and its cellulase hyper-producing mutants generated by heavy ion mutagenesis reveal the key genes involved in cellulolytic enzymes regulation","authors":"Miaoyin Dong, Shu-Yang Wang, Fuqiang Xu, G. Xiao, Jin Bai, Junkai Wang, Xisi Sun","doi":"10.1186/s13068-022-02161-7","DOIUrl":"https://doi.org/10.1186/s13068-022-02161-7","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45027276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic modifications of critical regulators provide new insights into regulation modes of raw-starch-digesting enzyme expression in Penicillium","authors":"Shengfang Zhao, Boyu Xiang, Le Yang, Jie Chen, Cui Zhu, Yu Chen, Jun Cui, Shengbiao Hu, Yibo Hu","doi":"10.1186/s13068-022-02162-6","DOIUrl":"https://doi.org/10.1186/s13068-022-02162-6","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46175562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic, proteomic and lactylated proteomic analyses indicate lactate plays important roles in maintaining energy and C:N homeostasis in Phaeodactylum tricornutum","authors":"Ai-you Huang, Yuanxiang Li, Jiawen Duan, Shiyi Guo, Xiaoni Cai, Xiang Zhang, H. Long, Wei Ren, Zhenyu Xie","doi":"10.1186/s13068-022-02152-8","DOIUrl":"https://doi.org/10.1186/s13068-022-02152-8","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45430712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering the oleaginous yeast Candida tropicalis for α-humulene overproduction","authors":"Lihua Zhang, Haiquan Yang, Yuanyuan Xia, W. Shen, Liming Liu, Qi Li, Xianzhong Chen","doi":"10.1186/s13068-022-02160-8","DOIUrl":"https://doi.org/10.1186/s13068-022-02160-8","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44027547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion","authors":"Sunghwa Woo, H. Lim, Y. Han, Sungwoo Park, M. Noh, D. Baek, J. Moon, S. Seo, G. Jung","doi":"10.1186/s13068-022-02157-3","DOIUrl":"https://doi.org/10.1186/s13068-022-02157-3","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44308393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning and comparative genomics approaches for the discovery of xylose transporters in yeast.","authors":"Mateus Bernabe Fiamenghi, João Gabriel Ribeiro Bueno, Antônio Pedro Camargo, Guilherme Borelli, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimarães Pereira, Leandro Vieira Dos Santos, Juliana José","doi":"10.1186/s13068-022-02153-7","DOIUrl":"10.1186/s13068-022-02153-7","url":null,"abstract":"<p><strong>Background: </strong>The need to mitigate and substitute the use of fossil fuels as the main energy matrix has led to the study and development of biofuels as an alternative. Second-generation (2G) ethanol arises as one biofuel with great potential, due to not only maintaining food security, but also as a product from economically interesting crops such as energy-cane. One of the main challenges of 2G ethanol is the inefficient uptake of pentose sugars by industrial yeast Saccharomyces cerevisiae, the main organism used for ethanol production. Understanding the main drivers for xylose assimilation and identify novel and efficient transporters is a key step to make the 2G process economically viable.</p><p><strong>Results: </strong>By implementing a strategy of searching for present motifs that may be responsible for xylose transport and past adaptations of sugar transporters in xylose fermenting species, we obtained a classifying model which was successfully used to select four different candidate transporters for evaluation in the S. cerevisiae hxt-null strain, EBY.VW4000, harbouring the xylose consumption pathway. Yeast cells expressing the transporters SpX, SpH and SpG showed a superior uptake performance in xylose compared to traditional literature control Gxf1.</p><p><strong>Conclusions: </strong>Modelling xylose transport with the small data available for yeast and bacteria proved a challenge that was overcome through different statistical strategies. Through this strategy, we present four novel xylose transporters which expands the repertoire of candidates targeting yeast genetic engineering for industrial fermentation. The repeated use of the model for characterizing new transporters will be useful both into finding the best candidates for industrial utilization and to increase the model's predictive capabilities.</p>","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47748255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions","authors":"Ru Chen, Miao Yang, Mengjie Li, Hao Zhang, Haiyan Lu, Xiaotan Dou, Shiqi Feng, Song Xue, Chenba Zhu, Zhanyou Chi, Fantao Kong","doi":"10.1186/s13068-022-02154-6","DOIUrl":"https://doi.org/10.1186/s13068-022-02154-6","url":null,"abstract":"","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41577324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and comparative transcriptome analyses reveal the mechanisms underlying waterlogging tolerance in a rapeseed anthocyanin-more mutant.","authors":"Li-Na Ding, Rui Liu, Teng Li, Ming Li, Xiao-Yan Liu, Wei-Jie Wang, Yan-Kun Yu, Jun Cao, Xiao-Li Tan","doi":"10.1186/s13068-022-02155-5","DOIUrl":"10.1186/s13068-022-02155-5","url":null,"abstract":"<p><strong>Background: </strong>Rapeseed (Brassica napus) is the second largest oil crop worldwide. It is widely used in food, energy production and the chemical industry, as well as being an ornamental. Consequently, it has a large economic value and developmental potential. Waterlogging is an important abiotic stress that restricts plant growth and development. However, little is known about the molecular mechanisms underlying waterlogging tolerance in B. napus.</p><p><strong>Results: </strong>In the present study, the physiological changes and transcriptomes of germination-stage rapeseed in response to waterlogging stress were investigated in the B. napus cultivar 'Zhongshuang 11' (ZS11) and its anthocyanin-more (am) mutant, which was identified in our previous study. The mutant showed stronger waterlogging tolerance compared with ZS11, and waterlogging stress significantly increased anthocyanin, soluble sugar and malondialdehyde contents and decreased chlorophyll contents in the mutant after 12 days of waterlogging. An RNA-seq analysis identified 1370 and 2336 differently expressed genes (DEGs) responding to waterlogging stress in ZS11 and am, respectively. An enrichment analysis revealed that the DEGs in ZS11 were predominately involved in carbohydrate metabolism, whereas those in the am mutant were particularly enriched in plant hormone signal transduction and response to endogenous stimulation. In total, 299 DEGs were identified as anthocyanin biosynthesis-related structural genes (24) and regulatory genes encoding transcription factors (275), which may explain the increased anthocyanin content in the am mutant. A total of 110 genes clustered in the plant hormone signal transduction pathway were also identified as DEGs, including 70 involved in auxin and ethylene signal transduction that were significantly changed in the mutant. Furthermore, the expression levels of 16 DEGs with putative roles in anthocyanin accumulation and biotic/abiotic stress responses were validated by quantitative real-time PCR as being consistent with the transcriptome profiles.</p><p><strong>Conclusion: </strong>This study provides new insights into the molecular mechanisms of increased anthocyanin contents in rapeseed in response to waterlogging stress, which should be useful for reducing the damage caused by waterlogging stress and for further breeding new rapeseed varieties with high waterlogging tolerance.</p>","PeriodicalId":93909,"journal":{"name":"Biotechnology for biofuels and bioproducts","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46321129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}