Dental research and oral health最新文献

{"title":"Comparative Effects of Cigarette Smoke and E-Cigarette Vapor on Oral Squamous Cell Carcinoma: Unraveling Distinct Molecular Pathways.","authors":"Ryan Kinney, Logan Ponder, Neha Patel, Ankita Chatterjee, Kristina Vu, Harishma Sidhu, Benjamin Bikman, Paul R Reynolds, Juan A Arroyo","doi":"10.26502/droh.0098","DOIUrl":"10.26502/droh.0098","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) poses a significant global health burden, driven by cigarette smoking and complicated by the rising popularity of e-cigarettes. This study compares the effects of cigarette smoke extract (CSE) and e-cigarette vapor extract (EVE) on OSCC progression using Ca9-22 gingival OSCC cells. CSE significantly increased cell invasion by upregulating the receptor for advanced glycation end-products (RAGE), nuclear factor kappa B (NF-κB), and matrix metalloproteinases (MMP-9, MMP-13), facilitating extracellular matrix degradation and metastasis. Conversely, the effects of EVE varied by flavor and nicotine content. Red Hot EVE (cinnamaldehyde-based) enhanced invasion and NF-κB levels with nicotine while reducing MMP-9 expression, suggesting alternative invasion pathways via MMP-13. Green Apple EVE had a less pronounced impact, indicating flavor-specific bioactivity. These findings reveal distinct mechanisms: CSE drives RAGE-NF-κB-MMP-mediated invasion, whereas EVE elicits variable, flavor-dependent responses, potentially involving nicotinic acetylcholine receptors. Targeted therapies, such as RAGE or NF-κB inhibitors, could mitigate tobacco-related OSCC progression. With e-cigarette use surging among youth, stricter regulations on flavored products and robust public health campaigns are urgently needed. Future research should investigate chronic exposures and diverse OSCC models to refine clinical and regulatory approaches, addressing the evolving landscape of tobacco-related oral cancers.</p>","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"8 3","pages":"97-103"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ChatGPT to Enhance Learning in Dental Education at a Historically Black Medical College.","authors":"Khandoker Rahad, Kianna Martin, Ihunna Amugo, Shania Ferguson, Angela Curtis, Anniya Davis, Pandu Gangula, Qingguo Wang","doi":"10.26502/droh.0069","DOIUrl":"10.26502/droh.0069","url":null,"abstract":"<p><p>The recent rise of powerful large language model (LLM)-based AI tools, exemplified by ChatGPT and Bard, poses a great challenge to contemporary dental education. It simultaneously offers a unique resource that potentially complements today's teaching and learning, where existing widely available learning resources have often fallen short. Although the LLM tools will shape both the clinical and educational aspects of dentistry profoundly, the didactic curricula, which primarily rely on lecture-based courses where instructors impart knowledge through presentations and discussions, need to be upgraded urgently. In this paper, we used dental course materials, syllabi, and textbooks adopted currently in the School of Dentistry (SOD) at Meharry Medical College to assess the potential utility and effectiveness of ChatGPT in dental education. We collected the responses of the chatbot to questions as well as students' interactions with it for assessment. Our results showed that ChatGPT can assist in dental essay writing and generate relevant content for dental students, in addition to other benefits. The limitations of ChatGPT were also discussed in the paper.</p>","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"7 1","pages":"8-14"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10887427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139974929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediatric Oral Health Service Access in Racial/Ethnic Minority Neighborhoods: A Geospatial Analysis in Washington, D.C., USA.","authors":"Minxuan Lan, Eric Niu, Meirong Liu, Sadiyah Anderson, LaToya Barham, Tanya Greenfield, Gail Cherry-Peppers, Xinbin Gu","doi":"10.26502/droh.0073","DOIUrl":"10.26502/droh.0073","url":null,"abstract":"<p><p>Oral health plays a crucial role in overall well-being. One of the goals set by the US Department of Health and Human Service, Healthy People 2030 is to reducing dental caries in children and adolescents. The study aims to investigate the accessibility of pediatric dental care in neighborhoods with mixed-race and predominantly African American populations in the Washington District of Columbia (DC) area. Our objective is to uncover and highlight the disparities that exist in pediatric dental care within these communities. We have specifically examined the geographic and socio-demographic aspects of pediatric dental care facilities, utilizing geospatial tools such as modeling and mapping, as well as data from the clinical database at Howard University College of Dentistry. The detailed analysis of dental care access revealed significant disparities among various Wards in the region. Specifically, Wards 5, 7, and 8 stood out as having both the highest concentrations of African American residents and the lowest availability of pediatric dentistry providers when compared to the more affluent Wards 1, 2, and 3. Howard University College of Dentistry's pediatric dentistry department played a crucial role in providing dental care services to the community. Over the course of the year 2022, they recorded a total of 3,855 visits from residents of the DC area. Notably, a substantial portion of these visits, specifically 1,566 visits, were from individuals residing in Wards 5, 7 and 8. This data underscores the significant demand for pediatric dental services in these underserved communities and highlights the importance of addressing the disparities in access to care.</p>","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"7 1","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11027513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Topoisomerase II alpha Inhibition on Oral Cancer Cell Metabolism and Cancer Stem Cell Function.","authors":"Thanigaivelan Kanagasabai, Mariam Hawaz, Kayla Ellis, Orlyne Fah, Helana Mikhaeil, Philip Nguyen, Nathalie Tombo, Anil Shanker, Chethan Sampath, Zaid H Khoury, James Cade, Alexys Ferguson, Pandu Gangula","doi":"10.26502/droh.0076","DOIUrl":"10.26502/droh.0076","url":null,"abstract":"<p><strong>Background: </strong>Topoisomerase IIα (TOP2A), is an enzyme involved in DNA replication, transcription, recombination, and chromatin remodeling and is found in a variety of cancers. However, the role of TOP2A regulation in oral cancer progression is not fully explained. We investigated the effect of TOP2A inhibition on cell survival, metabolism, and cancer stem cell self-renewal function in oral cancer cells.</p><p><strong>Methods: </strong>Oral carcinoma cell line SCC25 was cultured in complete DMEM/F12 media and treated with 5μM of Etoposide (Topoisomerase II inhibitor) for 48h. The critical parameters of cellular metabolism, including extracellular acidification rate (ECAR) and mitochondrial oxidative phosphorylation based on the oxygen consumption rate of cancer cells were assessed using Seahorse assay. Western blotting was performed to assess the proteins that are associated with proliferation (Survivin, IL-6) and cancer stem cell function (Oct4, Sox2) in cell lysates prepared from control and etoposide treated groups. Statistical analysis was performed using One-way ANOVA with Dunnett's multiple comparisons test.</p><p><strong>Results: </strong>The protein expression of TOP2A was significantly (P<0.05) inhibited by etoposide. Additionally, TOP2A inhibition decreased the mitochondrial respiratory parameters including basal respiration, maximal respiration and ATP production. However, TOP2A inhibition has no impact on glycolytic function. Moreover, the proliferative marker survivin and IL-6 showed a significant (P<0.05) decrease after TOP2A inhibition. Conversely, the protein expression of cancer stem cell markers Oct-4 and Sox 2 were not altered.</p><p><strong>Conclusion: </strong>These results indicate that inhibition of TOP2A is more efficacious by decreasing the mitochondrial metabolic reprogramming and thereby downregulating the key anti-apoptotic and pro-survival mediators. Thus, TOP2A represents an ideal therapeutic target and offers a potential treatment strategy for OSCC.</p>","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"7 2","pages":"58-65"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11218736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerous Distinct Orthodontic Treatment Techniques Impacting The Airway Dimensions: A Critical Review","authors":"Heba Allah Ahmed Ahmed Elghawy, Abbadi Adel Elkadi, Walaa El sayed Elgemeay","doi":"10.26502/droh.0056","DOIUrl":"https://doi.org/10.26502/droh.0056","url":null,"abstract":"","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69345788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parity, Maternal-Child Oral Interactions and Early Childhood Caries (ECC) among Northern Nigerian Hausa children","authors":"Elizabeth O Oziegbe, Lynne A Schepartz","doi":"10.26502/droh.0060","DOIUrl":"https://doi.org/10.26502/droh.0060","url":null,"abstract":"","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69345811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schematic Analysis and Fiducial Point of Tooth for Root Canal Therapy","authors":"D. Kim, Mustafa Aldabbagh, S. R. Makins, Helder B Jacob, Julian N Holland, Ji Wook Jeong","doi":"10.26502/droh.0055","DOIUrl":"https://doi.org/10.26502/droh.0055","url":null,"abstract":"","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69345737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of wearing a mouthguard on physical ability is dependent on occlusal contact state: A study involving elite level female handball players","authors":"Mutsumi Takahashi, Yogetsu Bando, Katsuhiko Kitaoka, Keisuke Hata","doi":"10.26502/droh.0066","DOIUrl":"https://doi.org/10.26502/droh.0066","url":null,"abstract":"The purpose of this study was to clarify the effects of wearing a mouthguard on the physical performance of handball players, depending on their individual occlusal contact state. Participants were 15 elite-level female handball players (25.7±3.2 years). Occlusal contact state was measured and divided into two groups; stable group and unstable group. Physical fitness test consisted of 8 items evaluating agility, explosive power, muscle strength, jumping ability, and flexibility, namely, step 50, pro-agility, three-cone drill, medicine ball scoop throw, vertical jump, back muscle strength, spread legs, and prone upper body arched. These tests were conducted under two conditions: not wearing and wearing a custom mouthguard. Scores of each test were analyzed using a split plot design, with occlusal balance and mouthguard as factors. In the stable group, scores on any of the tests were not affected by wearing a mouthguard. In the unstable group, wearing a mouthguard significantly improved test scores except for spread legs and prone upper body arched. The results of this study revealed that the effect of wearing a mouthguard on a handball player’s physical ability is influenced by the player’s occlusal contact state. In athletes with poor occlusal contact, improving occlusal contact by wearing a mouthguard had a positive impact on physical performance, mainly in terms of agility, explosive power, muscle strength, and jumping ability. However, for athletes who already had good occlusal contact, wearing a mouthguard did not affect their physical performance.","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"180 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135506650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel cancer associated fibroblasts from diverse locales enhance the tumorigenicity of tongue tumor epithelia","authors":"Nehanjali Dwivedi,","doi":"10.26502/droh.0064","DOIUrl":"https://doi.org/10.26502/droh.0064","url":null,"abstract":"Due to high rates of tobacco chewers, smokers, and alcohol consumers in India, Head and Neck Squamous Cell Carcinoma (HNSCC) is one of the primary causes of mortality. Being profoundly varied in nature, treating patients diagnosed with HNSCC can be difficult. An invitro cell line model is needed to better comprehend the heterogeneity especially via the interaction with components of the microenvironment like Cancer Associated Fibroblasts (CAFs). The effectiveness of creating cell lines from head and neck cancers is, however, poor. Furthermore, except for the two reported earlier by the authors, no other immortalized CAF cell lines are available to study the cross-talk of the tumor with its microenvironment. In this study, the authors report three novel CAF lines, spontaneously immortalized, from Human Papilloma Virus (HPV) negative male patients with habits of tobacco and diagnosed with squamous cell carcinoma of the upper alveolus, larynx and buccal mucosa. Negative staining with EpCAM, CD31 and CD45, while positive staining with FSP-1 determined their fibroblast specific lineage. Interestingly, in indirect co-culture experiments all three CAFs, though isolated from tumors of different regions of the oral cavity, could increase the tumorigenicity of the epithelial cells from tongue-tumor indicating at a common “CAF-factor”. The developed CAF cell lines are the first of their kind from the mentioned sites, and can act as invaluable tools for learning the site-independent common language between tumor-stroma and tumor in HNSCC.","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135059749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Skeletal Muscle Mass and Occlusal State on Flight Time of Straight Jumps in Trampoline Competition","authors":"Mutsumi Takahashi, Yogetsu Bando, Takuya Fukui","doi":"10.26502/droh.0062","DOIUrl":"https://doi.org/10.26502/droh.0062","url":null,"abstract":"The aim of this study was to clarify the effects of skeletal muscle mass and occlusal state on flight time of straight jumps in the trampoline competition. Participants were 11 elite-level trampoline male athletes (18.9 ± 0.9 years). A body composition analyzer was used to measure skeletal muscle mass, the left–right difference in the muscle mass of the lower extremities and ratio of trunk muscle mass to whole-body muscle mass were calculated. A pressure-sensitive film was used to measure the occlusal state, and the left-right difference in occlusal force was obtained. The trial consisted of 10 consecutive straight jumps, and the flight time was measured. Pearson’s product-moment correlation coefficient was used to analyze the correlations between flight time and the left–right difference in lower-extremity muscle mass, the ratio of trunk muscle mass to whole-body muscle mass, and the left–right difference in occlusal force. Significant negative correlation was observed between the flight time and the left-right difference in lower extremity muscle mass or occlusal force. Significant and strong positive correlation was observed between flight time and the ratio of trunk muscle mass to whole-body muscle mass. The results of this study suggest that the flight time of straight jumps in the trampoline competition is influenced by the balance of skeletal muscle mass and occlusal state. Therefore, the stability of the trunk supported by well-balanced posture muscles on the left and right sides as well as appropriate occlusal contact should contribute to improvements in flight time.","PeriodicalId":93742,"journal":{"name":"Dental research and oral health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69345362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}