Yueyao Wang, Dipankar Bandyopadhyay, John R Shaffer, Xiaowei Wu
{"title":"Gene-Based Association Mapping for Dental Caries in The GENEVA Consortium.","authors":"Yueyao Wang, Dipankar Bandyopadhyay, John R Shaffer, Xiaowei Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Dental caries is a multifactorial disease with high prevalence in both children and adults. Recent genome-wide association studies (GWASs) have revealed that genetic factors play an important role in caries incidence. However, existing methods are not sufficient to identify caries-associated genes, due to the complex correlation structure of caries GWAS data, and lack of appropriate summarization at the gene level. This paper attempts to address that by analyzing data from the Gene, Environment Association Studies (GENEVA) consortium.</p><p><strong>Methods: </strong>We investigated gene-based genetic associations for dental caries based on genome-wide data derived from the GENEVA database, with adjustment to covariates, linkage disequilibrium among single-nucleotide polymorphisms, and family relations, in sampled individuals.</p><p><strong>Results: </strong>Several suggestive genes were identified, in which some of them have been previously found to have potential biological functions on cariogenesis.</p><p><strong>Conclusions: </strong>By comparing the gene sets identified from gene-based and SNP-based association testing methods, we found a non-negligible overlap, which indicates that our gene-based analysis can provide substantial supplement to the traditional GWAS analysis.</p>","PeriodicalId":93308,"journal":{"name":"Journal of dentistry and dental medicine","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39521032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taira Kobayashi, Satoshi Uchibori, Osamu Tsuzukibash, Chihiro Uezato, M. Fuchigami, Haruhiko Goto, H. Tamaki, Koji Umezawa, Takaaki Tanaka, M. Ohta
{"title":"Novel Selective Medium for the Isolation of Rothia aeria, Which Is an Inhabitant of the Human Oral Cavity","authors":"Taira Kobayashi, Satoshi Uchibori, Osamu Tsuzukibash, Chihiro Uezato, M. Fuchigami, Haruhiko Goto, H. Tamaki, Koji Umezawa, Takaaki Tanaka, M. Ohta","doi":"10.31021/jddm.20181124","DOIUrl":"https://doi.org/10.31021/jddm.20181124","url":null,"abstract":"Roth aeria, Rothia dentocariosa, and Rothia mucilaginosa are isolated from the human oral cavity. Among them, R. aeria can cause severe systemic infection diseases (e.g., bronchitis, endocarditis, pneumonia, and sepsis). However, the veritable prevalence of this organism in the human oral cavity has not ever been known. Thus, the selective medium for the isolation and quantification of R. aeria is necessary to assess the prevalence of this organism, and to diagnose various R. aeria infection diseases. To investigate R. aeria distribution in oral cavities, a novel selective medium (RAESM) was developed for isolating and quantifying R. aeria. RAESM consists of sodium gluconate, tryptone, meat extract, sodium fluoride, acriflavin neutral, fosfomycin, lincomycin, colistin, aztreonam, and agar. Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 16S rDNA genes of R. aeria. The percentage of R. aeria in saliva samples collected from 20 subjects was examined. Moreover, we examined the antibiotic susceptibility of thirty isolates from six subjects. The average growth recovery of R. aeria on RAESM was 96.6% compared with that on Brain Heart Infusion supplemented with Yeast Extract (BHI-Y) agar. Growth of other representative oral bacteria, including other Rothia species, was remarkably inhibited on the selective medium. The PCR primers reacted to R. aeria and did not react to other Rothia species or representative oral bacteria. R. aeria was detected as 1.0% of the total bacteria, 5.9×107CFU/ml, on BHI-Y agar in the oral cavities of all subjects. R. aeria isolates obtained in this study were susceptible to most antibiotics; however R. aeria isolates from one subject were highly resistant to erythromycin, lincomycin, and clindamycin. R. aeria may be a part of the normal flora in the human oral cavities. A novel selective medium, RAESM, was useful for isolating R. aeria. Moreover, it was indicated that RAESM was useful for diagnosing R. aeria infections.","PeriodicalId":93308,"journal":{"name":"Journal of dentistry and dental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49281870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The First Time in","authors":"Nirmala Svsg, S. Degala, Minor Babu M.S","doi":"10.31021/JDDM.20181122","DOIUrl":"https://doi.org/10.31021/JDDM.20181122","url":null,"abstract":"• Teach in online master’s and graduate certificate program. Courses are 8-weeks in length, teach 10 courses annually. • Responsible for curriculum planning, course design, continuous development of instructional practice, and maintaining an updated knowledge of college student development and higher education. • Courses developed and taught: o EDG 6362 College Student Development o EDG 6366 Student Affairs and Administrative Services o EDG 6367 Foundation of Academic Advising o EDG 6361 American Higher Education o EDSD 6302 Advising for College Transitions o EDSD 6304 Program Administration and Assessment o EDG 6305 Legal and Ethical Principles o EDG 6364 Issues in Higher Education • Other courses taught: o EDG 6324 Career & Occupational Counseling o EDG 6301 Social and Cultural Influences o EDG 6369 Assessment of Student Outcomes o EDG 6398 Capstone in Student Development","PeriodicalId":93308,"journal":{"name":"Journal of dentistry and dental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43852728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The First Time in","authors":"X. Riaud","doi":"10.31021/JDDM.20181121","DOIUrl":"https://doi.org/10.31021/JDDM.20181121","url":null,"abstract":"","PeriodicalId":93308,"journal":{"name":"Journal of dentistry and dental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43992604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fanar Turki A.Al-Jadwaa, Moataz Gh Al-shaekh Ali, A. Taqa
{"title":"The Effect of Curing Time on the Degree of Conversion of Poly-acid Modifed Composite Resin (Compomer) �An In Vitro study","authors":"Fanar Turki A.Al-Jadwaa, Moataz Gh Al-shaekh Ali, A. Taqa","doi":"10.31021/jddm.20181118","DOIUrl":"https://doi.org/10.31021/jddm.20181118","url":null,"abstract":"Aims to evaluate the effect of light intensity on the degree of conversion of polyacidmodified composite resin. Materials and methods: Fourteen samples were prepared and divided randomly to four groups according to Light Intensity. Fourier Transform Infrared Spectrometer (FTIR) is used in order to measure the degree of conversion. Results and discussion: Analysis of variance (ANOVA) and Duncan multiple range test were used for analysis. The study showed that at 1mm depth the polyacid-modified composite resin has a difference in the degree of conversion at different intensities. As the light intensity increased, the degree of conversion measure was increased.","PeriodicalId":93308,"journal":{"name":"Journal of dentistry and dental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47657332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Flvio de Mendona Copello, D. Alviano, M. M. Souza, A. Bolognese, Angela Rita Pontes Azevedo
{"title":"Microbial Infection of Orthodontic Synthetic Intermaxillary Elastics in Differents Types of Manipulation: An In Vitro Study","authors":"Flvio de Mendona Copello, D. Alviano, M. M. Souza, A. Bolognese, Angela Rita Pontes Azevedo","doi":"10.31021/jddm.20181117","DOIUrl":"https://doi.org/10.31021/jddm.20181117","url":null,"abstract":"Background: Intermaxillary elastic is considered important sources of energy in orthodontic movement. However, the majority of this type of material, mainly those of Brazilian brands, is sold in packets containing 1000 units, which obliges the orthodontist or assistant to manipulate these, in order to separate them into a smaller quantity for each patient. The care taken by the working team is essential for preserving the integrity of the health of both the team and patients. Aim/objectives: the purpose of this study was to perform an in vitro evaluation of synthetic intermaxillary elastics from different manufacturers, to verify the presence of microorganisms at the time of opening the package and its application, by different forms of manipulation. Methods: 120 synthetic intermaxillary elastics were divided into four groups according to different trademarks and type of manipulation. These were incubated at 37 o C and evaluated after time intervals of 24 hours, 48 hours, 72 hours, 4 days, 7 days and 10 days. The cultures were analyzed by visual inspection and when the culture medium presented turbidity, the presence of contamination was considered. Findings/results: Bacterial contamination was only found in the groups that occurred hand manipulation of the materials. Discussion: The elastics tested in this study presented no evidence of microbial contamination before manipulation; manipulation of the elastics with the use of gloves and sterile forceps prevented contamination by microorganisms; the elastic manipulation with hands is not recommended.","PeriodicalId":93308,"journal":{"name":"Journal of dentistry and dental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49105093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}