Journal of general and molecular virology最新文献

Field deployable Reverse TranscriptaseRecombinase Polymerase Amplification (RT-RPA) for detection of Maize chlorotic mottle virus (MCMV) 田间可部署逆转录组合酶聚合酶扩增(RT-RPA)检测玉米绿斑病毒(MCMV)

Journal of general and molecular virology Pub Date : 2022-04-30 DOI: 10.5897/jgmv2022.0082

M. F., G. M., M. S., M. X., N. B., M. S. L.

{"title":"Field deployable Reverse TranscriptaseRecombinase Polymerase Amplification (RT-RPA) for detection of Maize chlorotic mottle virus (MCMV)","authors":"M. F., G. M., M. S., M. X., N. B., M. S. L.","doi":"10.5897/jgmv2022.0082","DOIUrl":"https://doi.org/10.5897/jgmv2022.0082","url":null,"abstract":"Diagnosis of maize lethal necrosis (MLN)-causing viruses is key in MLN surveillance programs and in testing seed for zero tolerance of Maize chlorotic mottle virus (MCMV) in seed lots. This is crucial for MLN management in farmers’ fields and in commercial seed fields. A customized MCMV detection assay that is specific, sensitive, affordable, and portable is therefore important for this task. Reverse Transcriptase – Recombinase Polymerase Amplification (RT-RPA) meets those conditions earlier described. RPA is a rapid isothermal nucleic acid amplification and detection platform that is based on patented Recombinase Polymerase Amplification (RPA) technology. In this study, a real time endpoint analysis and field deployable RT-RPA diagnostic method for the detection of MCMV was developed. RPA primer sets with their complementary probes were designed, synthesized and tested through a series of primer set evaluations to determine the most efficient primer sets. The primer sets targeted the MCMV genome at position 2765-2948 bp (MCMV_gp2 replicase gene). The parameters evaluated were sensitivity, specificity and reproducibility for the assay with remarkable results. The assay discriminated against other maize infecting viruses hence specific to MCMV. The assay takes only 20 min and its detection limit of 10 -4 is well comparable to RT-PCR and other molecular based detection assays. MCMV was also detected directly from leaf saps without the nucleic acid extraction step hence suitable for on-farm testing. RPA is a relatively inexpensive technique that requires minimal instrumentation. This assay is therefore suitable for the detection of MCMV in field surveys, routine MCMV testing for phytosanitary measures and in the seed certification procedures.","PeriodicalId":93204,"journal":{"name":"Journal of general and molecular virology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44002684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maize lethal necrosis and the molecular basis of variability in concentrations of the causal viruses in co-infected maize plant. 玉米致死性坏死病和共感染玉米植株中致病病毒浓度变异的分子基础。

Journal of general and molecular virology Pub Date : 2019-01-01 Epub Date: 2019-06-30 DOI: 10.5897/JGMV2019.0073

L A O Awata, B E Ifie, P Tongoona, E Danquah, M B Jumbo, M Gowda, P W Marchelo-D'ragga, Chelang'at Sitonik, L M Suresh

{"title":"Maize lethal necrosis and the molecular basis of variability in concentrations of the causal viruses in co-infected maize plant.","authors":"L A O Awata, B E Ifie, P Tongoona, E Danquah, M B Jumbo, M Gowda, P W Marchelo-D'ragga, Chelang'at Sitonik, L M Suresh","doi":"10.5897/JGMV2019.0073","DOIUrl":"10.5897/JGMV2019.0073","url":null,"abstract":"Maize lethal necrosis (MLN) disease is new to Africa. First report was in Kenya in 2012, since then the disease has rapidly spread to most parts of eastern and central Africa region including Tanzania, Burundi, DRC Congo, Rwanda, Uganda, Ethiopia and similar symptoms were observed in South Sudan. Elsewhere, the disease was caused by infection of Maize Chlorotic Mottle Virus (MCMV) in combination with any of the potyviruses namely; maize dwarf mosaic virus (MDMV), sugarcane mosaic virus (SCMV) and tritimovirus wheat streak mosaic virus (WSMV). In Africa, the disease occurs due to combined infections of maize by MCMV and SCMV, leading to severe yield losses. Efforts to address the disease spread have been ongoing. Serological techniques including enzyme-linked immuno-sorbent assay (ELISA), polymerase chain reaction (PCR), genome-wide association (GWAS) mapping and next generation sequencing have been effectively used to detect and characterize MLN causative pathogens. Various management strategies have been adapted to control MLN including use of resistant varieties, phytosanitary measures and better cultural practices. This review looks at the current knowledge on MLN causative viruses, genetic architecture and molecular basis underlying their synergistic interactions. Lastly, some research gaps towards MLN management will be identified. The information gathered may be useful for developing strategies towards future MLN management and maize improvement in Africa.","PeriodicalId":93204,"journal":{"name":"Journal of general and molecular virology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38766408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral Proliferation and Replication 病毒增殖与复制

Journal of general and molecular virology Pub Date : 2013-08-12 DOI: 10.1007/978-3-642-20718-1_3