Bioscientifica proceedings最新文献_第5页

Developmental programming of the ovine placenta 绵羊胎盘发育程序

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.7.004

A. Fowden, J. Ward, F. Wooding, A. Forhead

引用次数: 0

Porcine pluripotent stem cells and their differentiation 猪多能干细胞及其分化

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0028

T. Ezashi, R. M. Roberts

{"title":"Porcine pluripotent stem cells and their differentiation","authors":"T. Ezashi, R. M. Roberts","doi":"10.1530/biosciprocs.19.0028","DOIUrl":"https://doi.org/10.1530/biosciprocs.19.0028","url":null,"abstract":"In some situations, the pig has advantages over the mouse as a model in biomedical research. The availability of pluripotent cell lines is likely to broaden this appeal. Here we review progress in the derivation and characterization of embryonic stem cells (ESC) and induced pluripotent lines (iPSC) from pigs. Until recently, most porcine ESC failed to meet the full criteria for pluripotency, but that may be changing as more becomes known about the culture conditions required to maintain epiblast outgrowths from early porcine conceptuses in an undifferentiated, self-renewing state. In addition, porcine iPSC cells have been generated, some with the features of FGF2-dependent epiblast-type cells, typified by human ESC, and others that require LIF and resemble the “ground state”, na ï ve-type mouse ESC. Despite these successes, incomplete reprogramming and loss of pluripotency when selection conditions are relaxed continue to be problems that must be overcome if the full potential of iPSC is to be realized. The most immediate value of iPSC may relate to their ability to proliferate almost indefinitely in culture, thus enabling more complex genetic manipulations of the genome through growth selection than could be performed in other cell types. The “undifferentiated” state of iPSC may also allow improved cloning efficiency, although this remains to be proved. Finally, the pig will likely prove useful in testing stem cell-based therapies, although only a limited number of experiments demonstrating that the porcine iPSC can be directed to transform into more specialized sub-lineages and then form functional grafts have been performed.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46729242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The metabolism of the ruminant cumulus-oocyte complex revisited 反刍动物卵丘-卵母细胞复合体的代谢

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.8.022

Jeremy G. Thompson, R. Gilchrist, M. Sutton-Mcdowall

引用次数: 3

Next Generation Sequencing for microRNA profiling in the porcine endometrium 猪子宫内膜microRNA分析的下一代测序

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0019

K. Krawczynski, S. Bauersachs, H. Blum, M. Kaczmarek

引用次数: 0

Judge, jury and executioner: the auto-regulation of luteal function 法官、陪审团和刽子手:黄体功能的自动调节

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.6.012

G. Niswender, T. Davis, Griffith Rj, R. L. Bogan, K. Monser, Rebecca C. Bott, J. Bruemmer, Terry M. Nett

引用次数: 42

Current progress in non-surgical embryo transfer with fresh and vitrified/warmed pig embryos 用新鲜和玻璃化/温热猪胚胎进行非手术胚胎移植的最新进展

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0010

E. Martinez, E. A. Gil, C. Cuello, J. Sanchez-Osorio, J. Gomis, I. Parrilla, M. Angel, H. Rodríguez-Martínez, X. Lucas, J. Vazquez, J. M. Vázquez, J. Roca

{"title":"Current progress in non-surgical embryo transfer with fresh and vitrified/warmed pig embryos","authors":"E. Martinez, E. A. Gil, C. Cuello, J. Sanchez-Osorio, J. Gomis, I. Parrilla, M. Angel, H. Rodríguez-Martínez, X. Lucas, J. Vazquez, J. M. Vázquez, J. Roca","doi":"10.1530/biosciprocs.19.0010","DOIUrl":"https://doi.org/10.1530/biosciprocs.19.0010","url":null,"abstract":"Embryo transfer (ET) should play a critical role in the pig industry because it allows the movement and introduction of new genetic material into a herd with minimal risk of disease transmission and reduced transportation costs. In addition, embryo movement could prevent the potential health and welfare problems associated with transporting live pigs. Although the first successful ET was reported more than 60 years ago, the commercial use of this procedure in pigs is still in its infancy. The surgical requirements for embryo collection and transfer and the difficulties with embryo cryopreservation have prevented its use in pigs, unlike other livestock. However, new methodologies have been developed in the past decade to enable successful non-surgical ET and embryo cryopreservation that could allow the commercial use of ET in the pig industry. This review focuses on the development of these technologies with emphasis on our own findings. Specifically, we discuss the basic aspects of a non-surgical deep-uterine ET procedure and describe several factors that affect its efficacy in the transfer of fresh and short-term cultured embryos. Finally, we conclude with a brief discussion on the use of this procedure with long-term stored embryos.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48537948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Gene networks in the embryo and endometrium related to embryo survival 胚胎和子宫内膜中与胚胎存活相关的基因网络

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.8.006

M. Hoelker, D. Salilew-Wondim, E. Held, D. Tesfaye, K. Schellander

{"title":"Gene networks in the embryo and endometrium related to embryo survival","authors":"M. Hoelker, D. Salilew-Wondim, E. Held, D. Tesfaye, K. Schellander","doi":"10.1530/BIOSCIPROCS.8.006","DOIUrl":"https://doi.org/10.1530/BIOSCIPROCS.8.006","url":null,"abstract":"Low fertility is one of the major problems limiting the efficiency of the dairy industry. Successful pregnancy establishment and development to term depend on the suitability of the endometrial environment and the intrinsic quality of the embryo. Accurate assessment of both, however, is challenging. Therefore, the present survey reviews current knowledge of the molecular networks in the endometrium related to pregnancy establishment and maintenance to term. Accordingly, distinct molecular signatures of the bovine endometrium, elucidated by innovative approaches, could be correlated with pregnancy success. However, not only must the reproductive tract provide a suitable environment but the embryo itself must be competent to express its developmental program. Numerous studies have correlated molecular networks of bovine embryos with their developmental capacity. However, most studies were, by their nature, invasive and the findings were therefore difficult to extrapolate beyond the developmental stages examined. Hence, a second aim is to present new strategies employing embryo biopsies for a direct connection between molecular signatures and embryo developmental capacity. Large scale differences, at the molecular level, were reported for blastomeres whose counterparts developed to the blastocyst stage compared to those with lower developmental competence, thus unraveling distinct molecular fingerprints related to ability to develop to the blastocyst stage. Similarly, distinct molecular signatures at the blastocyst stage were associated with embryo developmental competence to term. In summary, further understanding of molecular signatures related to endometrial receptivity and embryo developmental capacity has been gained from novel innovative strategies including embryo microdissection as well as by examining endometrial samples collected in the cycle preceding the one in which conception occurs.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49029984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining piglet survival 仔猪存活率的测定

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0014

E. Baxter, S. Edwards

引用次数: 6

Novel concepts about normal sexual differentiation of reproductive neuroendocrine function and the developmental origins of female reproductive dysfunction: the sheep model 关于生殖神经内分泌功能正常性分化和女性生殖功能障碍发育起源的新概念：绵羊模型

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.6.006

D. Foster, L. Jackson, V. Padmanabhan

引用次数: 0

Steroidogenesis and the initiation of parturition 甾体形成和分娩的开始

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.8.028

A. Conley, L. Reynolds

{"title":"Steroidogenesis and the initiation of parturition","authors":"A. Conley, L. Reynolds","doi":"10.1530/BIOSCIPROCS.8.028","DOIUrl":"https://doi.org/10.1530/BIOSCIPROCS.8.028","url":null,"abstract":"One of the most fundamental axioms of mammalian reproduction is that pregnancy requires the support of progesterone without which it cannot be established or maintained. Though this basic physiological tenet was accepted long ago, major gaps in our understanding of the physiology of both pregnancy and parturition remain which hamper our ability to solve clinically and agriculturally significant problems such as low fertility, fetal growth restriction, preterm birth and poor neonatal outcomes. The historical reliance of our understanding of both pregnancy and parturition on this single hormone, and how it has been measured in the vast majority of studies, may represent a tangible weakness and impediment to progress. Other weaknesses include a desire to fit all species into a unified paradigm, and a reluctance to accept that physiological processes regulated by progesterone or other progestins in different tissues might vary in reliance on classic (nuclear receptor) versus other, non-classical mechanisms of action. The relative importance of these distinct response pathways in certain cells or tissues also may differ across species, as does so much of basic reproductive physiology. It is well known that certain species are reliant on luteal function throughout gestation, whereas the placenta subsumes endocrine support in others (Geisert & Conley 1998), yet progesterone alone is still believed to be the single common element. As radical as it might seem, however, progesterone may not be the single common hormone of pregnancy in mammals.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41582090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6