Biomarkers and applications最新文献_第2页

Improved Performance of CSF Dementia Biomarker Measurements Over Time: The Effect of Quality Control and Harmonization Programs 随着时间的推移脑脊液痴呆生物标志物测量性能的提高:质量控制和协调程序的影响

Biomarkers and applications Pub Date : 2018-07-05 DOI: 10.29011/2576-9588.100026

Maria Bourbouli, E. Kapaki, O. Petropoulou, G. Paraskevas

{"title":"Improved Performance of CSF Dementia Biomarker Measurements Over Time: The Effect of Quality Control and Harmonization Programs","authors":"Maria Bourbouli, E. Kapaki, O. Petropoulou, G. Paraskevas","doi":"10.29011/2576-9588.100026","DOIUrl":"https://doi.org/10.29011/2576-9588.100026","url":null,"abstract":"Introduction: Cerebrospinal Fluid (CSF) biomarkers Total Tau Protein (τT), Amyloid Beta Peptide (Aβ42) and tau protein hyper phosphorylated at threonine 181 (τP-181) are important in the (differential) diagnosis of dementia and analysis of these biomarkers is now incorporated in the diagnostic criteria of Alzheimer’s disease. However, lack of standardization has led to considerable interand intra-laboratory variation. Various international programs have been launched aiming in the reduction of variability and harmonization of biomarker measurements. Objectives: To explore whether experience gained from international quality control and harmonization programs had any effect on the analytical performance of our laboratory for CSF dementia biomarkers [Total Tau (τT), Amyloid Beta (Aβ42) and PhosphoTau (τP-181)]. Methods: We retrospectively analyzed internal standard measurements during ELISA runs in 3 time periods: before 2010, 2010-2012 (experience from workshops and quality control programs) and after 2012 (JPND-BIOMARKAPD harmonization program). Results: During the 1st period, coefficients of variation were 8.6%-17.1%. Subsequently, they were reduced, reaching 4.5%6.6% at the 3rd period. Measurement error was reduced for τT and Aβ42 from 9.2% and 22.1% to 1% and 3.3% respectively. Median values for Aβ42 were significantly lower compared to the expected values during the 1st period but, came closer to (at the 2nd period) and finally reached the expected value at the 3rd period. Conclusion: The improvement noted, indicates a beneficial effect of quality control and harmonization programs on analytical performance, by lowering measurement errors to levels which are not expected to adversely affect diagnostic performance in every day practice. . DOI: 10.29011/BMAP-126. 100026","PeriodicalId":93081,"journal":{"name":"Biomarkers and applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43892112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The Bone Regenerative Effects of the Biofield Energy Treated Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63) 生物场能量处理的维生素D3对人骨肉瘤细胞（MG-63）的骨再生作用

Biomarkers and applications Pub Date : 2018-06-25 DOI: 10.29011/2576-9588.100025

J. Peoples, M. Trivedi, A. Branton, Dahryn Trivedi, G. Nayak, Sambhu Mondal, S. Jana

{"title":"The Bone Regenerative Effects of the Biofield Energy Treated Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63)","authors":"J. Peoples, M. Trivedi, A. Branton, Dahryn Trivedi, G. Nayak, Sambhu Mondal, S. Jana","doi":"10.29011/2576-9588.100025","DOIUrl":"https://doi.org/10.29011/2576-9588.100025","url":null,"abstract":"Author(s): Peoples, James Jeffery; Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis | Abstract: The current research work was presented to evaluate impact of Biofield Energy Treated vitamin D3 and DMEM on bone health in human bone osteosarcoma cells (MG-63). The Test Items (TI), were distributed into two parts. One part of each sample was received Consciousness Energy Healing Treatment by James Jeffery Peoples and labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as Untreated Test Items (UT). Test samples were found as safe in tested concentrations by MTT assay. ALP was significantly increased by 54.43% and 111.24% at 10 and 100 µg/mL, respectively in BT-DMEM + UT-TI than UT-DMEM + UT-TI. Moreover, ALP was significantly elevated by 128.14%, 77.84%, and 62.28% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 1 µg/mL compared to untreated. Collagen was significantly increased by 286.67% and 340% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 0.1 µg/mL, while increased by 134.08% in UT-DMEM + BT-TI at 10 µg/mL than untreated. Besides, percent of bone mineralization was remarkably increased by 140.94%, 113.72%, and 129.87% at 1 µg/mL in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively, while increased by 187.91% in BT-DMEM + UT-TI at 0.1 µg/mL than untreated. Altogether, Biofield Treated vitamin D3 was significantly improved the bone growth and it could be able to fight against various bone-related disorders (osteoporosis, osteogenesis imperfecta, Paget’s disease, rickets, osteomalacia), autoimmune and inflammatory diseases, stress management, and anti-aging by improving overall health.","PeriodicalId":93081,"journal":{"name":"Biomarkers and applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45653150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Three Innate Cytokine Biomarkers Predict Presence of Acute Otitis Media and Relevant Otopathogens. 三种先天细胞因子生物标志物预测急性中耳炎和相关耳病原体的存在。

Biomarkers and applications Pub Date : 2018-01-01 Epub Date: 2018-02-07 DOI: 10.29011/2576-9588.100018

Michael E Pichichero, Matthew C Morris, Anthony Almudevar

{"title":"Three Innate Cytokine Biomarkers Predict Presence of Acute Otitis Media and Relevant Otopathogens.","authors":"Michael E Pichichero, Matthew C Morris, Anthony Almudevar","doi":"10.29011/2576-9588.100018","DOIUrl":"https://doi.org/10.29011/2576-9588.100018","url":null,"abstract":"Background: 1.1Diagnosis of Acute Otitis Media (AOM) is challenging, resulting in frequent over diagnosis and improper prescription of antibiotics. A serum biomarker of AOM would significantly improve pediatric care for this common illness.Methods: 1.2Serum samples were studied from 197 children 6-36 months old during health, during viral Upper Respiratory Infection (URI) without middle ear involvement, and at the onset of AOM (confirmed by tympanocentesis). Serum concentrations of S100A12, IL-10, and ICAM-1 were measured by ELISA. Otopathogens were identified by culture of middle ear fluid. A predictive model for infection and causative otopathogen was developed based on density distributions of the measured cytokines.Results: 1.3A biomarker score derived from subject age and serum concentrations of S100A12, IL-10, and ICAM-1 was significantly able to distinguish both between health and disease and between upper respiratory infections with and without middle ear involvement (AOM vs URI), and further predicted the specific causative bacterial pathogen. This biomarker could also identify recurrent OM-prone children.Conclusions: 1.4For the first time we show that a biomarker risk score derived from serum cytokine levels can predict the presence of bacterial AOM, the likely Otopathogen, and the recurrent OM-prone child.Clinical significance: 1.5(1) AOM is a widespread pediatric infection with a substantial economic burden. (2) Three serum cytokines can discriminate between URI and AOM, reducing over diagnosis. (3) Prediction of responsible pathogen enables targeted antibiotic prescription.","PeriodicalId":93081,"journal":{"name":"Biomarkers and applications","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8734035/pdf/nihms-1002079.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39678881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Glymphatic Efficiency Is a Critical Factor for Using Abnormal Tau in Peripheral Tissues as Biomarker for Alzheimer's Disease. 将外周组织中的异常 Tau 用作阿尔茨海默病的生物标记物，淋巴效率是一个关键因素。

Biomarkers and applications Pub Date : 2018-01-01 Epub Date: 2018-12-28 DOI: 10.29011/2576-9588.100030

Yahuan Lou, Colin Carlock, Jean Wu

{"title":"Glymphatic Efficiency Is a Critical Factor for Using Abnormal Tau in Peripheral Tissues as Biomarker for Alzheimer's Disease.","authors":"Yahuan Lou, Colin Carlock, Jean Wu","doi":"10.29011/2576-9588.100030","DOIUrl":"10.29011/2576-9588.100030","url":null,"abstract":"Alzheimer's Disease or other dementias are characterized by the accumulation of abnormal tau and amyloid β peptides in brains. Therefore, abnormal tau and amyloid peptides in peripheral tissues or blood have been explored as diagnostic biomarkers. On the other hand, recent studies have revealed glymphatics a special drainage system for brain's wastes. We aimed to investigate whether effectiveness of glymphatic system affects the quantity of abnormal tau in the peripheral tissues. We have previously shown that aged IL33 KO (Il33 -/-) mice develop Alzheimer's like disease. Despite a large quantity of abnormal tau in brains, Il33 -/- mice showed a much lower amount of abnormal tau drained to the peripheral tissues kidneys than in wild type mice. Our further study showed that it was caused by defective glymphatic drainage since Il33 KO impaired glymphatics. Thus, it is necessary to identify biomarkers, which can evaluate efficiency of glymphatic drainage. Simultaneous measurement of these biomarkers and abnormal tau in peripheral tissues or blood may be critical for accurate diagnosis of Alzheimer's disease.","PeriodicalId":93081,"journal":{"name":"Biomarkers and applications","volume":"2018 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729990/pdf/nihms-1043241.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38705845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0