Bioactive compounds in health and disease最新文献

{"title":"Biochemical properties of several genetic resources of the national tomato germplasm","authors":"Karine Sarikyan, Marine Grigoryan, G. Shaboyan, Meruzhan Zadayan, Gohar Kirakosyan","doi":"10.31989/bchd.v7i1.1305","DOIUrl":"https://doi.org/10.31989/bchd.v7i1.1305","url":null,"abstract":"The tomato (Lycopersicon esculentum Mill.) is a self-pollinated diploid species with twelve pairs of chromosomes (2n = 24). It belongs to the Solanaceae family, along with other frugally important crops such as pepper, eggplant, and potato. Tomatoes are a rich source of vitamins and minerals (Ca, P, and Fe) and are strong antioxidants against cancer and heart disease. It is a leading vegetable crop in our republic and is widely cultivated in open and protected soils. Due to the biologically active substances and essential amino acids contained in the fruit, it is used in the daily diet of people throughout the year in fresh and “processing” form. The tomato plays an important role in the prevention of diseases in humans, and many varieties and hybrids belonging to its different varieties are currently being created by breeders. In our republic, tomato selection work started more than 90 years ago. Various cultivated varieties were created, which are widely cultivated by farmers. During the creation of the variety, great attention was paid to their taste and quality characteristics. The created varieties are of national value, and it is important to preserve them for future generations as well as for cultivation and selection purposes. Given the impact of climate change and varying environmental conditions on the biochemical indicators of tomato fruits, we performed these studies to get more accurate data on the fruits of different varieties of tomatoes under genotype x environment and genotype x year cultivation conditions.Objective: To evaluate the efficiency of several genetic resources of the national tomato germplasm in terms of the content of bioactive components and qualitative parameters (dry matter, sugars, acidity, vitamin C, B group vitamins.) in tomato fruits.Methods:Experiments were carried out at v. Darakert, Ararat Marz, Republic of Armenia, in 2021–2022. A block-randomized method was used to set up the experiment in four replications. Experiments were conducted on various genetic resources of the national tomato, including Fresh, “Processing”, Cherry, and Datterini varieties. The study examined bioactive compounds, dry matter, and total sugars in biologically ripened tomato fruits. Ascorbic acid was determined through titration, and B vitamins were quantified using spectrophotometric analysis on a Cary 60 UV-Vis spectrophotometer. The content of vitamin B was calculated using a calibration graph.Results: The study revealed that tomato fruits contain high levels of dry matter, sugars, acidity, vitamin C, and B group vitamins, with pantothenic acid, nicotinic acid, and inositol being predominant.Conclusion:Several genetic resources of national tomato: Yerevani 14, Noviy Anahit 19, Haykakan shtambovi 152, Masisi 202, Zvartnots, Noy (fresh tomato varieties), Renesans, Marine (“processing” tomato varieties), Zhanna, Rubina, Zeytun (cherry and datterini tomato varieties) have high levels of dry matter, sugars, acidity, vitamin C, B group","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"704 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140479520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study the effect of labneh balls fortified with zinc salts on the proportions of nitrogenous substances, micro-textural structure, and some nutritional indicators","authors":"Firas Najm Ismael, Suhair Ali Hussein, Omar Salah Ahmed Almuharib, Kifah Saed Abbas Doosh, Sara Thamer Hadi","doi":"10.31989/bchd.v7i1.1267","DOIUrl":"https://doi.org/10.31989/bchd.v7i1.1267","url":null,"abstract":"Background: Labneh is a dairy product with many of the same benefits as yogurt. The total solids percentage is raised during production by draining the whey out of the yogurt. This is done by hanging the yogurt in cloth bags until the desired texture is achieved. Labneh, after being sweetened, can be used in place of cream cheese, sour cream, or cake cream. Labneh is known to have fewer calories than common condiments such as cream cheese and sour cream. This is due to its reduced fat content Labneh is a popular cuisine all over the world, particularly in the Middle East and the Balkan nations. In conclusion, the effect of fortification was clear in the results of the nutritional experiment conducted on mice, as zinc salts had an effect in maintaining the weight gain of the experimental animals, in addition to a role in strengthening the immune system.Objective: This study aimed to explore the effects of enhancing traditional labneh cheese with mineral salts—specifically sulfate and zinc acetate—at concentrations of 7.5 and 15 mg/kg. The focus was on evaluating the alterations in chemical composition, particularly nitrogenous compounds, and the micro-textural properties. Additionally, the study assessed how these enhancements influenced the sensory qualities of the labneh both immediately after production and following a storage period of twenty days.Materials and Methods: Raw, whole cow's milk (12% T.S., 4% fat) was used to make labneh in the lab. After being heated to 90 °C for five minutes in a water bath, the milk was cooled to 45 °C, and a CH1 starter was added. The mixture was then incubated at 45 °C for three to four hours. The cloth was allowed to cool at room temperature for one hour after it had coagulated completely. After chilling overnight at 15 degrees Celsius, it was salted at 5 degrees Celsius for 22 hours, by placing it in a clean cloth bag and storing it in the fridge.Results:The experiment revealed a non-significant rise in nitrogenous substances for the control group versus the zinc-treated labneh. However, there were no notable differences in nitrogen content between the control and zinc-enhanced groups. The treatment labneh balls were less white than the control treatment balls, suggesting that the fortification process with zinc salts caused this difference. Increased cohesion and hardness of the manufactured Labneh compared to the control treatment had an apparent effect on the micro-textural structure of the treated Labneh, as the interstitial spaces were reduced, and the casein tissue closed. In addition to its role in bolstering the immune system, the results of the nutritional experiment on rats showed that zinc salts influence maintaining the weight increase of the trial animals.Keywords: Labneh balls, zinc salts, texture, flavor, chemical composition, body weight.","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"109 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139605867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytomineral complexes as elements of functional nutrition to enhance the protective function of the lymphatic system in aging","authors":"V. Gorchakov, Kirill M. Nicolaychuk, O. Gorchakova, Georgi Demchenko, B. Nurmakhanova","doi":"10.31989/bchd.v7i1.1289","DOIUrl":"https://doi.org/10.31989/bchd.v7i1.1289","url":null,"abstract":"Background: The problem of aging remains relevant in the modern world, with a notable connection between aging and nutrition. It is important to find a way to slow down aging. Integration lymphology with functional food theory (or phytodietics) is the most promising idea. Detection of lymphotropic properties in bioactive food ingredients will increase the lymphatic system activity in aging. Our task is to improve the protective status of lymph nodes by taking a phytomineral product in aging. Objective: The research purpose is to provide evidence of the use of a plant-mineral complex to activate the lymph system (lymph nodes) in aging. Participants in the experiment are 160 Wistar rats divided into groups of old and young animals. Half of the aging animals took an additional food plant-mineral complex \"IQdetoxSORB\". The basis of the food complex is physiologically acting substances such as flavonoids, trace elements, food fibers, and others.Methods: Different methods have been applied to study systems forming components of lymph nodes. The system of lymph node compartments was examined by a histological method. The liquid component of the organ is evaluated by a thermogravimetric method. Lymph node trace elements (Mn, Fe, Cu, Zn, Se) were determined by X-ray fluorescence analysis using synchrotron radiation (RFA SR). The StatPlus Pro license program and statistical analysis of dynamic series were used to process digital data.Results: We first presented data on the effect of functional nutrition on the lymphatic system and proved the lymphotropic properties of the plant-mineral complex. The bioactive ingredients of the complex (flavonoids, trace elements, food fibers) are useful and influence the lymphatic system, improving health in old age. The increase in lymphatic system activity occurs through the implementation of a lymphosanation mechanism due to the stimulating and protective effects of plant ingredients. Intake of the phytomineral complex accelerates lymph flow, modifies lymph node compartments, and restores trace element balance. The positive effect depends on the formation of an association of bioelements with lymphoid compartments. A plant-based diet corrects and increases the protective status of lymph nodes. The result is a slowdown in age-induced changes in peripheral lymphoid organs. Conclusion: As an element of functional nutrition, the plant-mineral complex contains bioactive ingredients - flavonoids, trace elements, and food fibers. These bioactive substances alter the existing status of the lymphatic system, increasing protective potential. Increased activity of the lymph system (lymph nodes) is an argument for slowing aging and preventing age-associated pathology. The information presented is the basis for recommending plant-mineral complexes for wellness programs of anti-aging.Keywords:lymphatic system; lymph nodes; compartments; trace elements; gerontology, phytotherapy, nutriciology.","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":" 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139622186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pain and Inflammation Management: Part-II Clinical Investigation of a Topical Ayurvedic Cream called HerboCare or HerboJointTM","authors":"Binoy K. Bordoloi, Kulwant S. Saini, Bishnu P. Sarma, Kimjonlhing Jolly, Ronen K. Bordoloi, Raghuram Kandimalla, Rahul L. Gajbhiye, Sayantan Sengupta","doi":"10.31989/bchd.v6i12.1139","DOIUrl":"https://doi.org/10.31989/bchd.v6i12.1139","url":null,"abstract":"Background: Joint pain and chronic inflammation pose significant health challenges, particularly among the elderly population. Current treatments often offer only temporary relief and are associated with potential side effects.  Ayurvedic botanical herbs and oils, known for their phytochemical-rich, anti-inflammatory properties, hold promise in addressing these issues.Objective: This study aimed to investigate the clinical efficacy of HerboCare orHerboJoint™, a topical Ayurvedic cream, in alleviating muscle discomfort, inflammation, and joint pain, along with its impact on inflammatory cytokines, particularly TNF-α on human subjects. While HerboCare or HerboJoint had a very low content of Menthol, HerboJoint-Plus (not reported in this study) was formulated with a much higher content of USP grade Menthol.Methods: HerboCareor HerboJoint™ cream, formulated with essential oils from Cymbopogon citratus, Hedychium spicatum, Zanthoxylum alatum, and Menthol (Mentha arvensis), was evaluated in two sets of clinical trials over a 90-day period. Patients with joint pain were divided into different treatment groups, and their pain, swelling, stiffness, and inflammatory marker TNF-α were assessed.Results: In the first set of trials, HerboCare orHerboJoint™ significantly reduced joint pain (29%), joint swelling (31%), and joint stiffness (60%) in patients. In the second set, TNF-α levels were reduced by 38% in the HerboCare orHerboJoint™-only group, 63% in the HerboCare orHerboJoint™ and prescribed formulation group, and 71% in the group receiving only the prescribed formulation. These results underscored the cream's efficacy in mitigating inflammation.Conclusion: HerboCare orHerboJoint™, formulated with Generally Recognized as Safe (GRAS)-affirmed botanical ingredients, demonstrates clinical effectiveness in relieving joint pain and inflammation. This Ayurvedic approach offers a safe and cost-effective alternative for managing these debilitating conditions.Keywords: Muscular discomfort,Joint Pain, Essential Oils; Menthol; Inflammatory Cytokines; Clinical Study","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"33 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139010525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted extraction of phenolic compounds from Moringa oleifera leaves by response surface methodology","authors":"Eunice Tranquilino‐Rodríguez, H. Martínez‐Flores","doi":"10.31989/bchd.v6i11.1229","DOIUrl":"https://doi.org/10.31989/bchd.v6i11.1229","url":null,"abstract":"Background: Moringa oleifera is a tree that grows in tropical and subtropical areas around the world. Its leaves, seeds, bark, roots, and flowers are used as ingredients in meals and medicinal applications. Moringa leaf extracts have been studied to contain antioxidant compounds such as phenolic molecules. Recent extraction techniques such as ultrasound and microwaves are alternatives to increase the extraction performance of phenolic compounds while preserving their antioxidant activity.Objective: The approach consisted of optimizing, using a Composite Central Design of Response Surface Methodology (CCD-RMS), the process conditions, for ultrasound-assisted extraction (UAE) of antioxidant phenolic compounds from Moringa oleifera leaves. Methods: A 33 CCD-RMS was used; three independent variables were studied: ethanol concentration (25%, 50%, 75%), time (10, 20, and 30 min), and temperature (25, 37.5, and 50°C). The results showed that the optimal UAE conditions were an ethanol concentration of 52.4%, extraction time of 3.2 min, and temperature of 58.5°C; under these conditions the phenolic content was 13.92±0.21 mg GAE/g sample the total flavonoid content was 10.60±0.06 mg QE/g sample, and 52.22±2.01% and 72.81±1.58% of DPPH• and ABTS•+ radicals were inhibited, respectively.Conclusion: This optimization will make it possible to assess better use of M. oleifera leaves as an antioxidant ingredient in functional food development.Keywords: Moringa oleifera, composite central design, optimization, flavonoids, phenols, antioxidant activity.","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"940 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139204402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-density lipoprotein- cholesterol and its relation to epicardial fat volume in patients with type II diabetes mellitus","authors":"Asmaa A. Hasan, Ghada M. S. Ahmed, B. A. Abdel Hady, Inass H. Ahmed, Entesar O. El Saghier, Marwa F. M. El Sayed, L. Azel, Marwa A, Mohamed, Ola I. Saleh, R. S. E. Taha, Mona A. Raafat, Ola H Abd Elaziz","doi":"10.31989/bchd.v6i11.1235","DOIUrl":"https://doi.org/10.31989/bchd.v6i11.1235","url":null,"abstract":"Objectives: This study aimed to compare EFV between diabetic and non-diabetic subjects in patients with clinical indications of CCTA and test the correlation between low-density lipoprotein-cholesterol (LDL-C) and EFV in type 2 diabetic (T2DM) patients.Methods: This study was conducted on 103 cases with chest pain and intermediate risk probability for CAD and was scheduled for CT coronary angiography divided into 47 diabetic patients and 56 non-diabetic patients. The total serum cholesterol, LDL-C, TG, and HDL-C levels were analyzed for each patient. MDCT to assess CACS and EFV for patients included in the study. Results: The results showed thatplasma total cholesterol, TG, and LDL-C were higher with decreased HDL in the diabetic patient. EFV was significantly higher in diabetic patients (54.5±14.9 vs 44.7±7.7, p <0.02). EFV had a significant  Linear correlation with plasma total cholesterol, LDL-C, and TG. In contrast, there is a significant negative correlation between EFV and HDL-c. EFV was significantly correlated with ca score (EFV was higher in diabetic patients with greater CAC score). Conclusion: EFV is rising in type II diabetic patients, especially those with high ca scores, and correlates well to their characteristic hyperlipidemia, especially LDL-C. So, all diabetic patients must be started on primary prevention against LDL-C to reduce the risk of atherosclerosis. Keywords: Type II DM, Epicardial fat volume, LDL-c.","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":" 43","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139240603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The investigation of bioactive compounds in the Charentsi grape variety and its derived wines","authors":"Bella Grigoryan, Mikayel Mikayelyan","doi":"10.31989/bchd.v6i11.1170","DOIUrl":"https://doi.org/10.31989/bchd.v6i11.1170","url":null,"abstract":"Background: Grapes contain thousands of chemical compounds, including sugars, organic acids, phenolic compounds, minerals, organic nitrogen, amino acids, and aromatic compounds.Objectives: The study investigates the possibility of producing high-quality red wines from the Charentsi grape variety and observes the extraction of   bioactive compounds formed during fermentation.Results: The organic acids in grapes and wine were also studied.  There were significant differences in the compounds mentioned above between wine samples based on the yeasts used. Our observations showed that the amount of tartaric acid in the Charentsi grape variety was 7.26 g/l, and after fermentation by using different yeasts in the wine samples, the content of tartaric acid was: BSC:103 2.06 g/l, AC-4: 1.79 g/l. The grape sample contained 3.01g/l malic acid, while the selections of wine BCS103 and AC-4 contained 2.01g/l and 3.00g/l, respectively. In the case of citric acid, the grape contained 0.5 g/l a, while the wine samples with BCS103 and AC-4 yeasts contained 0.52 g/l and 0.57 g/l respectively.The grape sample did not contain lactic acid or succinic acid. Wine samples were fermented by a variety of newly formed yeasts. In BCS103 yeast, these values were 1.89 g/l and 1.63 g/l, and 1.3 g/l and 1.61 g/l in AC-4.The values of anthocyanin and flavonoids in Charentsi grape variety are 2084.5 mg/kg and 8828.6 mg/kg, respectively. Wine samples contained high quantities of these compounds as well. The anthocyanin and flavonoids concentrations In BCS103, were 842.7 mg/l and 4898.22 mg/l, while they were 783.3 mg/l and 5025.5 mg/l in AC-4. The total phenolic compounds detected in the wine samples were 4957.9 mg/l and 5102.6 mg/l, in BCS103 and AC-4 respectively. According to the analysis, 37%-40% of anthocyanin and 55-56% of flavonoids were transferred to the wine.Conclusion: Charentsi grapes and wine are highly functional due to their high phenolic content and organic acids. These compounds are biologically active and influenced by the yeast strain used for wine fermentation.Keywords: grapes, red wine, phenolic compounds, organic acids, bioactive compounds","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"2015 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139239329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor activity of chitin-glucan complex of basidiomycetes","authors":"A. Razin, E. Vorobeychikov, V. G. Konusova, M. M. Shamtsyan, V. E. Murzina, Y. M. Volkov","doi":"10.31989/bchd.v6i11.1224","DOIUrl":"https://doi.org/10.31989/bchd.v6i11.1224","url":null,"abstract":"Background:High-molecular β-D-glucans of basidiomycetes modify the immune response and indirectly affect antitumor mechanisms by stimulating the activity of various immune cells and signaling pathways. However, the severity of the immune activity of β-D-glucans depends on many factors, which indicates the need to study them to obtain standardized agents of various pathogenetic directions. Objective:Evaluation of the antitumor activity of the chitin-glucan complex in the form of aqueous suspensions obtained from the fungi Ganoderma lucidum, Grifola frondosa, and Phallus impudicus using the Ehrlich adenocarcinoma (ACE) model.Methods:The work was carried out on male F1(C57Bl×CBA) hybrid mice. Animals were transplanted subcutaneously with ACE at a dose of 106 cells in 0.5 ml of Hank's solution. Animals were given prophylactic oral administration of suspensions of fungi for 10 days before inoculation with ACE. After inoculation with ACE, suspensions of fungi were orally administered to animals of these groups for 28 days for therapeutic purposes. From the 6th day after transplantation of the tumor, its volume was recorded in mm3 according to three linear dimensions. Results:Survival in all groups of animals within 28 days after inoculation with ACE was 100%. Administration of aqueous suspensions of Phallus impudicus, Ganoderma lucidum, and Grifola frondosa fungi to animals with ACE compensated for metabolic disturbances in the body, which increased the dynamics of their weight gain. However, the weight of these animals on the 28th day of the experiment did not reach the weight of animals that were not transplanted with ACE. Against the background of the introduction of suspensions of these fungi to animals from 6 to 11 days, a statistically significant (p<0.05) effect of inhibition of the development of the ECA volume was obtained, which did not depend on the type of fungi studied.Conclusion:Aqueous suspensions of these fungi have a similar effect of inhibiting the development of ACE. This allows us to consider the chitin-glucan components of the studied mushrooms as substances with indirect antitumor activity for their standardization and optimization of their use for prophylactic or therapeutic purposes. Keywords:β-D-glucans, chitin-glucan complex, basidiomycetes, Ehrlich's adenocarcinoma.","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139247921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational study of antiviral, anti-bacterial, and anticancer activity of green-extracted Sidr (Ziziphus spina-Christi) fruit phenolics","authors":"Fadwa W. Abdulqahar, Zuhair I. Mahdi, Shaymaa H. M. Al-kubaisy, Feryal F. Hussein, Malikakhon Kurbonova, Marwa M. El-Said, Tamer M. El-Messery","doi":"10.31989/bchd.v6i10.1192","DOIUrl":"https://doi.org/10.31989/bchd.v6i10.1192","url":null,"abstract":"Background: Hepatitis may result in inflammation, swelling, cirrhosis, cancer, and failure. Food-born bacteria like Escherichia coli, Staphylococcus aureus, Vibrio cholera, and Helicobacter pylori have widely developed antibiotic resistance in recent years. The latter is a common hazardous pathogen that may lead to stomach vacuoles and gastric cancer. Despite the toxicity, medication resistance, and/ or financial burdens of conventional cancer medicines, most breast cancer patients globally develop recurrence or relapse distant metastases in many other organs after receiving initial common treatment. Such cases need to find solutions that differ from conventional medicines. Plants are an essential source of efficient bioactive compounds that could be utilized in fighting bacterial and viral infections and/or cancer safely. Due to their capability to control many molecular pathways with less harmful effects, the phytochemicals used in complementary medicine have recently attracted more attention. Most of these phytochemicals are members of one of the alkaloids, phenolics, carotenoids, flavonoids, and/ or terpenoids groups. Sidr (Ziziphus spina-Christi (L.) Willd) is a well-known, traditionally used fruit for curing many diseases in the Middle East. Network medicine and AI technologies can rapidly hasten the discovery of new drug alternatives. Objective: This study aimed to extract phenolic compounds from Sidr via two green chemistry approaches, then to predict the anticancer and the antimicrobial effect of the characterized phytochemicals towards certain microorganisms. Methods: Sidr fruit powder was extracted via supercritical CO2 and ethanol asco-solvent and the defatted residues were extracted via the hydro-ethanolic ultrasound assisted extraction to obtain a higher yield of phenolic compounds. The phenolic compounds were characterized and computationally studied for their biological activity against different protein targets of hepatitis virus, food- and water-borne pathogenic bacteria, and breast cancer via Molecular docking and Molecular Dynamics. Additionally, ADMET study was performed for the compounds with high Vina score and good dynamics results. Results: Chemical analysis revealed the presence of seven different phenolic compounds (Gallic acid, caffeic acid, Pyrocatechol, Coumaric acid, Ferulic acid, Quercetin, and Cinnamic acid). By the results obtained from molecular docking phytochemical Quercetin showed good binding scores and interactions with S. aureus tyrosyl-tRNA synthetase (1JIJ), V. cholera UDP-N-acetylenolpyruvoylglucosamine reductase (3I99), Human topoisomerase Ⅱ beta (3QX3) and H. pylori shikimate kinase (3N2E) with Vina scores equal -9.9, -9.4, -9.3 and -8.5 respectively. ADMET analysis showed that Quercetin obeyed Lipinski's rule and had high GI absorbance, no BBB, and low toxicity within limits. Molecular dynamics studies revealed that V. cholera (3I99), Human topoisomerase Ⅱ beta (3QX3), and H. pylori shikimate kinase ","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135366551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pain and Inflammation Management: Part-I Pre-clinical Study of a Topical Ayurvedic Cream called HerboJoint","authors":"Binoy K. Bordoloi, Kulwant S. Saini, Bishnu P. Sarma, Kimjonlhing Jolly, Ronen K. Bordoloi, Raghuram Kandimalla, Rahul L. Gajbhiye, Sayantan Sengupta","doi":"10.31989/bchd.v6i10.1138","DOIUrl":"https://doi.org/10.31989/bchd.v6i10.1138","url":null,"abstract":"Background: The properties of a system of essential oils derived from GRAS (Generally Recognized as Safe) medicinal plants were studied by drawing upon the wisdom of Ayurveda. The objective was to address muscle relaxation, stiffness, swelling and inflammation of human subjects.Objective: A novel formulation is reported here, namely Kattrna or Lemongrass (CC-Cymbopogon citratus) (3.0%), Sati (HS-Hedychium spicatum) (1.0%) and Tumuru (ZA- Zanthoxylum alatum) oil (1.0%) along with USP grade Menthol (which can also be derived from Mentha) (1 to 10%). This formulation, called HerboJointTM, was patented.Methods: Its composition was investigated via HPLC and GC-MS analysis by comparing it with identifiable marker components. Cell line studies were performed followed by a Type-II collagen-induced arthritic mice model investigation over a 21-day period of blood plasma of before vs. after treatment for the inflammatory markers.Results: The synergistic action of the selected essential oils and Menthol was demonstrated to reduce the inflammatory cytokines, including TNF-α, IL-6, and IL-1β, in the blood plasma of the treated arthritic mice. Conclusion: It is believed to provide significant relief against inflammation. Therefore, formulations were subsequently prepared in a non-greasy oil-in-water emulsion cream base for applying topically around the affected joint and other areas for a human clinical investigation. Keywords: Joint Pain; Essential Oils; Menthol; Inflammatory Cytokines; Pre-Clinical Study","PeriodicalId":93079,"journal":{"name":"Bioactive compounds in health and disease","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136079970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}