{"title":"Transcriptome-wide piRNA profiling in human brains for aging genetic factors.","authors":"Qiao Mao, Longhua Fan, Xiaoping Wang, Xiandong Lin, Yuping Cao, Chengchou Zheng, Yong Zhang, Huihao Zhang, Rolando Garcia-Milian, Longli Kang, Jing Shi, Ting Yu, Kesheng Wang, Lingjun Zuo, Chiang-Shan R Li, Xiaoyun Guo, Xingguang Luo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Piwi-interacting RNAs (piRNAs) represent a molecular feature shared by all nonaging biological systems, including the germline and somatic cancer stem cells, which display an indefinite renewal capacity and lifespan-stable genomic integrity and are potentially immortal. Here, we tested the hypothesis that piRNA is a critical genetic determinant of aging in humans.</p><p><strong>Methods: </strong>Expression of transcriptome-wide piRNAs (n=24k) was profiled in the human prefrontal cortex of 12 subjects (84.9±9.5, range 68-100, years of age) using microarray technology. We examined the correlation between these piRNAs' expression levels and age, adjusting for covariates including disease status.</p><p><strong>Results: </strong>A total of 9,453 piRNAs were detected in brain. Including seven intergenic and three intronic piRNAs, ten piRNAs were significantly associated with age after correction for multiple testing (|r|=0.9; 1.9×10<sup>-5</sup>≤p≤9.9×10<sup>-5</sup>).</p><p><strong>Conclusion: </strong>We conclude that piRNAs might play a potential role in determining the years of survival of humans. The underlying mechanisms might involve the suppression of transposable elements (TEs) and expression regulation of aging-associated genes.</p>","PeriodicalId":92917,"journal":{"name":"Jacobs journal of genetics","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059831/pdf/nihms-1047993.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37718370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}