Transcriptome-wide piRNA profiling in human brains for aging genetic factors.

Jacobs journal of genetics Pub Date : 2019-01-01 Epub Date: 2019-08-20

Qiao Mao, Longhua Fan, Xiaoping Wang, Xiandong Lin, Yuping Cao, Chengchou Zheng, Yong Zhang, Huihao Zhang, Rolando Garcia-Milian, Longli Kang, Jing Shi, Ting Yu, Kesheng Wang, Lingjun Zuo, Chiang-Shan R Li, Xiaoyun Guo, Xingguang Luo

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Abstract

Objective: Piwi-interacting RNAs (piRNAs) represent a molecular feature shared by all nonaging biological systems, including the germline and somatic cancer stem cells, which display an indefinite renewal capacity and lifespan-stable genomic integrity and are potentially immortal. Here, we tested the hypothesis that piRNA is a critical genetic determinant of aging in humans.

Methods: Expression of transcriptome-wide piRNAs (n=24k) was profiled in the human prefrontal cortex of 12 subjects (84.9±9.5, range 68-100, years of age) using microarray technology. We examined the correlation between these piRNAs' expression levels and age, adjusting for covariates including disease status.

Results: A total of 9,453 piRNAs were detected in brain. Including seven intergenic and three intronic piRNAs, ten piRNAs were significantly associated with age after correction for multiple testing (|r|=0.9; 1.9×10^-5≤p≤9.9×10^-5).

Conclusion: We conclude that piRNAs might play a potential role in determining the years of survival of humans. The underlying mechanisms might involve the suppression of transposable elements (TEs) and expression regulation of aging-associated genes.

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人类大脑中衰老遗传因素的转录组全piRNA谱分析。

目的:piwi相互作用rna (piRNAs)代表了所有非衰老生物系统共有的一种分子特征，包括种系和体细胞癌症干细胞，它们显示出无限的更新能力和寿命稳定的基因组完整性，并且可能是不朽的。在这里，我们验证了piRNA是人类衰老的关键遗传决定因素的假设。方法:采用微阵列技术对12名受试者(84.9±9.5岁，68-100岁，年龄)前额叶皮层中转录组全pirna (n=24k)的表达进行分析。我们检查了这些pirna表达水平与年龄之间的相关性，并调整了包括疾病状态在内的协变量。结果:在脑组织中共检测到9453个pirna。包括7个基因间pirna和3个内含子pirna，经多次检验校正后，10个pirna与年龄显著相关(|r|=0.9;这个赛季≤1.9×10 p≤9.9×纯)。结论:我们得出结论，pirna可能在决定人类生存年限方面发挥潜在作用。其潜在机制可能涉及转座因子(te)的抑制和衰老相关基因的表达调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Jacobs journal of genetics

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