Journal of undergraduate research (Gainesville, Fla.)最新文献

Taste sensitivity and consumption responses to sucrose and artificial sweeteners in Drosophila melanogaster and the impact of the microbiome. 黑腹果蝇对蔗糖和人工甜味剂的味觉敏感性和消费反应及其微生物组的影响。

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2025-01-01 Epub Date: 2025-11-05 DOI: 10.32473/ufjur.27.138791

Serina Kaochari, Allison Dehnel, Eric Ntiri, Adam C N Wong

{"title":"Taste sensitivity and consumption responses to sucrose and artificial sweeteners in Drosophila melanogaster and the impact of the microbiome.","authors":"Serina Kaochari, Allison Dehnel, Eric Ntiri, Adam C N Wong","doi":"10.32473/ufjur.27.138791","DOIUrl":"10.32473/ufjur.27.138791","url":null,"abstract":"Artificial sweeteners may alter taste sensitivity and feeding behavior, but their effects compared to natural sugars and the role of the gut microbiome in modulating these responses remain unclear. This study compares taste sensitivity and consumption responses to erythritol (Truvia), sucralose (Splenda), and sucrose in flies with a gut microbiome (conventional) and without a microbiome (axenic). Taste sensitivity was assessed using the proboscis extension response (PER) assay, while the capillary feeder (CAFE) assay was used to measure consumption. PER results revealed that sucrose elicited a significantly stronger taste response than Splenda or Truvia, with response rates increasing at higher concentrations. CAFE assays indicated fly aversion to Truvia, and axenic flies consuming significantly more Truvia compared to conventional flies, suggesting a significant microbiome effect. In the Splenda trials, axenic flies consumed significantly more yeast than those in the sucrose treatment, suggesting a potential compensatory feeding response due to the lack of calories in artificial sweeteners.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"27 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13284731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148310588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Grounded Theory Exploration of Air Taxis in Florida 佛罗里达州空中出租车的接地理论探索

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133141

Thais De Moraes Campello

{"title":"A Grounded Theory Exploration of Air Taxis in Florida","authors":"Thais De Moraes Campello","doi":"10.32473/ufjur.25.133141","DOIUrl":"https://doi.org/10.32473/ufjur.25.133141","url":null,"abstract":"This study investigates Floridians’ perspectives on an emerging mode of transportation: air taxis, technically known as e-VTOL (electric vertical take-off and landing). This qualitative study aimed to identify the primary motivations for fostering acceptance and adoption by the general public. Three significant themes and seven sub-themes were identified through a grounded theory exploration of 13 in-depth interviews. The main themes encompass trust, a sense of agency, and the prolongment of excitement. Initial findings indicate that trust needs to be gained from the public through education, a guarantee of safety, and credible communication to generate acceptance of this new technology. However, findings suggest that Floridians view air taxis as a one-time entertainment, which does not guarantee adoption. Prolonging excitement and making passengers feel a sense of agency over their lives and time indicates that it will lead to adoption. The findings suggest a communication strategy to promote e-VTOL should be based on the three themes to ensure acceptance and adoption. Florida's future mobility plans can benefit from this qualitative research to re-imagine future cities, as few studies have assessed or investigated public motivations around this innovation.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"71 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136033400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Landscape Assessment of North American Wave and Wind Facilities 北美风浪设施景观评价

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133117

Nicholas Mesa, Forrest Masters, Pedro Lomonaco

引用次数: 0

Teacher Stress, Teacher Unintentional Bias, and Teacher Well-Being Before and During COVID-19 在COVID-19之前和期间，教师压力、教师无意偏见和教师幸福感

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133319

None Zoe Primack, None Joni Splett, None Joe Graham

{"title":"Teacher Stress, Teacher Unintentional Bias, and Teacher Well-Being Before and During COVID-19","authors":"None Zoe Primack, None Joni Splett, None Joe Graham","doi":"10.32473/ufjur.25.133319","DOIUrl":"https://doi.org/10.32473/ufjur.25.133319","url":null,"abstract":"Almost half of all teachers in the United States report high levels of daily stress from teaching, making it one of the most stressful jobs in the country. Teacher stress is detrimental to one’s health and well-being, leading to poor academic outcomes for students and a hostile classroom environment, both barriers for young minds to learn. The COVID-19 pandemic and fear of personal health have exacerbated teacher stress levels. Individual factors that contribute to stress, such as being tired or making quick judgments, are linked to the tendency to rely on automatic cognitive processes (e.g., unintentional biases) rather than more controlled processes; these factors lead to discriminatory acts toward minoritized students. In the Vulnerable Decision Point model, the root causes of minoritized students' experiences of discriminatory acts of disproportionate discipline in schools are explained by the impact of stress on teachers’ automatic processing and unintentional racial bias (URB). Current research for teacher stress includes ways to identify and reduce stress but have only achieved moderate effect sizes driving the need to identify additional strategies for addressing teacher stress, such as teacher subjective well-being (SWB). SWB skills can include mindfulness, resiliency, and self-efficacy, thus providing opportunities to mitigate the effects of daily stressors on unintentional biases by being conscious of fast decision-making and escalation of unexpected behaviors. The data includes self-reports from middle school teachers who completed baseline demographic questionnaires and various scales prior to randomization and intervention implementation. This study aims to understand how preexisting conditions of teacher perceived stress and teacher SWB have perpetuated unintentional biases toward Black students and may have changed during the COVID-19 pandemic.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"1899 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135994436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Pol II Recruitment to the Human Beta-Globin Locus Control Region Super-Enhancer 人-珠蛋白基因座控制区超级增强子Pol II募集的机制

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133391

Breanna Johnson, Matthew Gibbons, Fabia Bushra, Yu Fang, Jorg Bungert

{"title":"Mechanisms of Pol II Recruitment to the Human Beta-Globin Locus Control Region Super-Enhancer","authors":"Breanna Johnson, Matthew Gibbons, Fabia Bushra, Yu Fang, Jorg Bungert","doi":"10.32473/ufjur.25.133391","DOIUrl":"https://doi.org/10.32473/ufjur.25.133391","url":null,"abstract":"The human ꞵ-globin gene locus consists of five genes that encode subunits of hemoglobin. The locus control region (LCR) regulates these genes, acting as a super-enhancer to promote high-level of β-globin expression in erythroid cells. The LCR, like other super-enhancers, is known to recruit transcription complexes and generate non-coding enhancer RNA (eRNA). Past research has suggested that super-enhancers form phase-separated transcription initiation domains that concentrate RNA polymerase II (Pol II) and transfer Pol II to target gene promoters during transient looping interactions. The aim of this project is to examine the role of TFIIH, involved transcription initiation, in enhancer RNA production and Pol II transfer in the globin gene locus. The LCR was immobilized on magnetic beads and incubated with erythroid nuclear protein extracts. Proteins not bound were washed away and bound proteins were subjected to western blotting experiments. The data show that TFIIH is bound at the LCR and thus may be involved in eRNA production. Cut & Run experiments were performed to examine the localization of TFIIH in the globin gene locus in intact cells. In the future, the laboratory plans to deplete TFIIH and examine production of eRNA and Pol II recruitment at the LCR.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135994580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of VIS-NIR Hyperspectral Imaging to the Detection and Quantification of MPs in Simulant Shellfish VIS-NIR高光谱成像在模拟贝类MPs检测与定量中的应用

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133111

Kendall Schlitt

{"title":"Application of VIS-NIR Hyperspectral Imaging to the Detection and Quantification of MPs in Simulant Shellfish","authors":"Kendall Schlitt","doi":"10.32473/ufjur.25.133111","DOIUrl":"https://doi.org/10.32473/ufjur.25.133111","url":null,"abstract":"As the world has become increasingly reliant on single-use plastics, plastic litter has increased exponentially, especially in marine ecosystems. One type of plastic litter affecting marine life is microplastics, which are plastics under 5mm in diameter. Microplastics are consumed by marine animals, which are then consumed by people. Study of the environmental and human health effects of these microplastics has proven difficult, in part due to difficulty detecting and quantifying microplastics. This study aims to detect microplastics in shellfish by utilizing the nondestructive method of hyperspectral imaging as an indicator of both marine ecosystem health and food quality. Gelatin models of shellfish were injected with a known concentration of microplastics (polyvinyl chloride (PVC) and polystyrene (PS), specifically). The hyperspectral camera then took 3-dimensional images of the models, with the wavelength dimension ranging from 400 nm to 1000 nm. Next, an algorithm called Partial Least Squares Regression Analysis was developed to analyze the relationship between the wavelengths and the amount of plastic in the sample. Preliminary results have shown slight variation in reflectance spectra between models spiked with microplastics and control models past the 950 nm range. Therefore, hyperspectral imaging might be a viable tool to detect and quantify microplastics in shellfish, but the range of the camera used in this study is a limitation.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136033521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vulnerable Patients with Inflammatory Bowel Disease 易受感染的炎症性肠病患者

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133020

Tomas Potlach, Ellen Zimmermann

{"title":"Vulnerable Patients with Inflammatory Bowel Disease","authors":"Tomas Potlach, Ellen Zimmermann","doi":"10.32473/ufjur.25.133020","DOIUrl":"https://doi.org/10.32473/ufjur.25.133020","url":null,"abstract":"Crohn’s Disease and ulcerative colitis, collectively known as Inflammatory Bowel Disease (IBD), are conditions characterized by inflammation and ulceration of the intestines. This work aims to highlight disparities among vulnerable IBD populations using the OneFlorida Clinical Research Consortium, a data network housing claims and electronic health record information for more than 15 million patients in Florida. A retrospective analysis using data collected between 01/01/2012 and 06/30/2020 was performed using OneFlorida, and patients were identified using diagnosis and pharmacy codes for IBD. 10,578 patients met the inclusion criteria. Healthcare utilization (emergency department visits, inpatient stay, medications, procedures), disease severity (c-reactive protein, white blood cell count, hemoglobin, albumin), and outcomes (i.e., abdominal surgery) were assessed for patients of different ages, gender, ethnicities, races, and locations. Notably, adolescent and young adult (AYA) patients (ages 17-25) use significantly more corticosteroids than both the pediatric and older adult groups (p-values 0.005 and 0.0007). Contrary to prior literature, Hispanic IBD patients used more biologic medications compared to non-Hispanic White patients (p<0.0001), whereas non-Hispanic Black patients used more steroids (p=0.0004). These findings illustrate a need for targeted educational interventions to support these vulnerable populations.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136033524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity Patterns of Howler Monkeys in Captivity 圈养吼猴的活动模式

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133125

Grant Dougherty

引用次数: 0

Monomania and Same-Sex Desires in Braddon's Lady Audley's Secret (1862) 布莱登《奥德利夫人的秘密》(1862)中的偏执狂与同性欲望

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133130

None Savannah Elaine Baggett

引用次数: 0

Dissecting the Role of SAG101 in Extracellular NAD(P)+-Mediated Immune Signaling 剖析SAG101在细胞外NAD(P)+介导的免疫信号传导中的作用

Journal of undergraduate research (Gainesville, Fla.) Pub Date : 2023-10-17 DOI: 10.32473/ufjur.25.133138

Emily Liu, Mingxi Zhou, Zhonglin Mou

{"title":"Dissecting the Role of SAG101 in Extracellular NAD(P)+-Mediated Immune Signaling","authors":"Emily Liu, Mingxi Zhou, Zhonglin Mou","doi":"10.32473/ufjur.25.133138","DOIUrl":"https://doi.org/10.32473/ufjur.25.133138","url":null,"abstract":"Plants are the primary source of food for all animals, but they are often infected by microbial pathogens, which reduces crop yield and threatens global food security. Therefore, plants have evolved sophisticated defense mechanisms to fend off microbial infections. EDS1, PAD4, and SAG101 are well-characterized plant immune regulators. While the EDS1-PAD4 complex was proven to be necessary for extracellular NAD(P) [eNAD(P)]-mediated immune signaling, the function of the EDS1-SAG101 complex in this pathway has yet to be determined. This research aimed to determine the necessity of SAG101 in the process of eNAD(P) signaling. Our hypothesis was that SAG101 formed a complex with EDS1 to carry out a necessary role in eNAD(P) signaling. Arabidopsis thaliana T-DNA insertion lines SALK_022911C and SALK_030411C were genotyped, and SALK_022911C was identified as a sag101 homozygous mutant. NAD(P)-induced immune responses in the sag101 mutant were compared to those in the eds1 and pad4 mutants. Exogenously applied NAD(P) induced expression of the defense marker genes, FMO1 and ALD1, as well as resistance to the bacterial pathogen Pseudomonas syringae pv. maculicola ES4326 in sag101. However, this induction was completely blocked in eds1 and pad4. These results demonstrate that the EDS1-SAG101 complex is not involved in the eNAD(P) signaling pathway and supports the model that the EDS1-PAD4 and EDS1-SAG101 complexes function differently in plant immunity.","PeriodicalId":92815,"journal":{"name":"Journal of undergraduate research (Gainesville, Fla.)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136033254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0