BULGARIAN JOURNAL OF VETERINARY MEDICINE最新文献_第2页

Evaluation of pelvic limb angles in dogs with medial patellar luxation: Comparison of radiography and computed tomography 髌骨内侧脱位犬骨盆肢体角度的评估:x线摄影和计算机断层摄影的比较

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2413

R. Garnoeva, М. Paskalev

引用次数: 0

Infections with Dicrocoelium dendriticum, Cysticercus pisiformis, Micipsella numidica and seroprevalence of Toxoplasma gondii in European brown hares (Lepus europeus L.) from Bulgaria

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2021-0025

A. Trifonova, M. Panayotova-Pencheva, E. Kaneva

{"title":"Infections with Dicrocoelium dendriticum, Cysticercus pisiformis, Micipsella numidica and seroprevalence of Toxoplasma gondii in European brown hares (Lepus europeus L.) from Bulgaria","authors":"A. Trifonova, M. Panayotova-Pencheva, E. Kaneva","doi":"10.15547/bjvm.2021-0025","DOIUrl":"https://doi.org/10.15547/bjvm.2021-0025","url":null,"abstract":"During the period 2009–2016, brown hares (Lepus europeus L.) from different regions of Bulgaria were investigated for infections with liver parasites as well as for seroprevalence of protozoan Toxoplasma gondii. Three helminth species were found in the liver: one trematode – Dicrocoelium dendriticum, one cestode – larval form of Taenia pisiformis – Cysticercus pisiformis and one nematode – Micipsella numidica. Dicrocoeliasis was found in all parts of the country with infected hares originating mainly from the western regions. The total prevalence of infection with D. dendriticum was 10.59%. The intensity of infection ranged from 1 to 184, 26.67 on the average. The total prevalence of infection with C. pisiformis was 16.8%. The prevalence of infection was highest among hares from the southern parts of the country, and no cysticercosis was registered in the western parts. The extent of infestation with cysticerci was different – from single cysticerci on the omentum or serosa of some organs in the abdominal cavity to innumerable ones that affected also the organs in the thoracic cavity. However, cases in which the animals were slightly affected, predominated. The total prevalence of infection with M. numidica was 5.31%, and the intensity of infection varied between 1 and 6 parasites in one animal. The nematodiasis was established mainly in hares from southern Bulgaria. The total seroprevalence of T. gondii was 6.15% and the protozoan-positive animals originated from three districts: Stara Zagora, Plovdiv, and Burgas. The current studies also showed that the prevalence of D. dendriticum infection among animals increased with age. No correlation was observed between prevalence of infection with C. pisiformis and M. numidica and host age. No correlation was found between the prevalence of the helminth infections and sex of the animals.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78397722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistin gene expression: Novel study in dromedary camel (Camelus dromedarius) 单峰骆驼(Camelus dromedarius)抵抗素基因表达新研究

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2021-0014

N. E. Abd Elhameed, R. Abdelaziz, M. Bakry, M. Hamada

引用次数: 0

Effects of metabiotic Bacillus subtilis for microbiocenosis formation in early-weaned piglets 代谢枯草芽孢杆菌对早期断奶仔猪微生物病形成的影响

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2021-0062

V. Popov, N. Naumov, G. Svazlyan

引用次数: 0

Identification and characterisation of lumpy skin disease virus recently isolated from Giza, Egypt 最近从埃及吉萨分离的肿块性皮肤病病毒的鉴定和特征

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2021-0008

C. Mikhael, H. Abd El Aziz, A. M. Ali

{"title":"Identification and characterisation of lumpy skin disease virus recently isolated from Giza, Egypt","authors":"C. Mikhael, H. Abd El Aziz, A. M. Ali","doi":"10.15547/bjvm.2021-0008","DOIUrl":"https://doi.org/10.15547/bjvm.2021-0008","url":null,"abstract":"Lumpy skin disease (LSD) is a viral disease, geographically distributed in Africa and now, vigorously spread in the Near East and also in Europe and Asia. It has a significant economic impact on cattle industry in Africa. The aim of this study was isolation and rapid identification of LSD virus circulating in Egypt from clinically suspected cattle based on clinical and molecular basis in a rapid and accurate way. Fifteen representative specimens (skin sitfasts) were collected in 2018 from clinically infected cattle in Giza governorate, Egypt. The virus was isolated on chorioallantoic membrane of specific pathogen free embryonated chicken eggs and Madin Darby Bovine Kidney tissue culture cells. The isolated virus was identified and confirmed by conventional polymerase chain reaction (PCR) and real-time PCR. Histopathological examination of the lesions showed a pathognomic intracytoplasmic inclusion body in dermal stroma section. The section of dermal layer revealed vasculitis with projection of its endothelial lining. It was concluded that LSD was enzootic in Egypt and still circulating among Egyptian cattle so that LSD virus could be isolated and identified by traditional and molecular diagnostic methodes. Real time PCR assay could be applied for rapid and accurate confirmation of the field isolate of LSD virus.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90292299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence, haematological, biochemical abnormalities and clinical syndromes of FeLV and FeLV/FIV co-infection among cat population in Moscow and the Moscow region, Russia 俄罗斯莫斯科和莫斯科地区猫群FeLV和FeLV/FIV合并感染的流行、血液学、生化异常和临床综合征

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2021-0001

A. P. Zenchenkova, V. Makarov, Bjvm

{"title":"Prevalence, haematological, biochemical abnormalities and clinical syndromes of FeLV and FeLV/FIV co-infection among cat population in Moscow and the Moscow region, Russia","authors":"A. P. Zenchenkova, V. Makarov, Bjvm","doi":"10.15547/bjvm.2021-0001","DOIUrl":"https://doi.org/10.15547/bjvm.2021-0001","url":null,"abstract":"The aims of this retrospective study were to determine the prevalence of FeLV and co-infection with FeLV and FIV among feline population of Moscow and the Moscow region, Russia, to determine the risk factors associated with the disease and to describe the clinical syndromes, haematological and biochemical abnormalities in infected cats. The prevalence of FeLV and FeLV+FIV co-infection was investigated among 11,807 cats living in Moscow and the Moscow region using PCR, ELISA and ICT methods. The overall prevalence of FeLV among household domestic cats was 12.8%. FeLV was most commonly detected in male mixed-breed cats and the cats with free outdoor access. Ninety cases of co-infection with FeLV and FIV (0.76% of the cat population) were also detected. The most common clinical signs seen in infected cats were those associated with the gastrointestinal tract. Anaemia was a common haematological abnormality in infected cats. The results of biochemical assays were nonspecific and associated with inflammatory processes in infected cats. The risk factors associated with FeLV infection were sex, breed, age, contacts with other cats and a history of outdoor access. The results presented in the study indicated a high prevalence of FeLV and a low prevalence of FeLV+FIV co-infection in cats living in Moscow and the Moscow region, Russia.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76790631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exertional rhabdomyolysis in a fallow deer (Cervus dama) 驼鹿(Cervus dama)劳力性横纹肌溶解

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2352

G. Simeonova, I. Kalkanov

引用次数: 0

A new solution for control of respiratory infection by Streptococcus equi isolated from horses in northern Iran 控制伊朗北部马链球菌呼吸道感染的新方法

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2020-0144

M. Yaghoobpour, L. Fozouni, P. Ghaemi

{"title":"A new solution for control of respiratory infection by Streptococcus equi isolated from horses in northern Iran","authors":"M. Yaghoobpour, L. Fozouni, P. Ghaemi","doi":"10.15547/bjvm.2020-0144","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0144","url":null,"abstract":"Respiratory infections caused by Streptococcus equi are one of the most important diseases in the equine industry. The increasing rate of antibiotic resistance among this bacterial species necessitates new antimicrobial agents. In this research, nasopharyngeal samples were taken from horses (n=90) in some areas of the Golestan Province, Iran. After isolation and identification of S. equi strains, antibiotic resistance of the isolates was evaluated using the Kirby-Bauer method. The antibacterial effect of lavender essential oil was investigated by broth microdilution assay. The frequency of streptococcal strains was 27.8%. The most frequent isolates were S. equi subsp. equi (72%) and S. equi subsp. zooepidemicus (28%). The frequency of S. equi was highest in horses aged less than 2 years (69.3%) and thoroughbred Turkmen horses (61.5%). The highest rate of resistance was observed against amoxicillin, while the highest rate of sensitivity was to ceftriaxone. Furthermore, the essential oil of lavender inhibited 90% of the isolates at concentrations ≥2048 μL/mL. Our study revealed that the frequency of antibiotic-resistant S. equi isolates was relatively high in the studied population of horses in Iran. It was concluded that essential oil of lavender possessed more potent in vitro activity than the common antibiotics for treatment of infections caused by S. equi subspecies.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89443714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

High expression of LipL21 protein of Iranian Leptospira interrogans in E. coli, applicable for diagnostic ELISA 伊朗钩端螺旋体LipL21蛋白在大肠杆菌中高表达，可用于ELISA诊断

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2021-0052

K. Kamalzadeh, M. Esmaelizad, J. Ghalani, P. Khaki, M. Tebianian

{"title":"High expression of LipL21 protein of Iranian Leptospira interrogans in E. coli, applicable for diagnostic ELISA","authors":"K. Kamalzadeh, M. Esmaelizad, J. Ghalani, P. Khaki, M. Tebianian","doi":"10.15547/bjvm.2021-0052","DOIUrl":"https://doi.org/10.15547/bjvm.2021-0052","url":null,"abstract":"Leptospirosis is an emerging infectious zoonotic disease caused by pathogenic Leptospira. The disease is more prevalent among farmers in hot and humid areas of Iran. Lack of clear clinical signs have impeded the diagnosis of leptospirosis. In this study, we attempted to produce a recombinant LipL21 protein of Leptospira based on a dominant pattern of Iranian isolates and to evaluate it in ELISA test. One hundred and sixty-two complete sequences of LipL21 available in GenBank until January 1, 2019 were compared. One dominant LipL21 protein pattern was selected. The codon optimised sequence was cloned into the pET32a+ expression vector. Trx-LipL21 fusion protein was induced, purified and confirmed by 10% SDS-PAGE followed Coomassie blue staining and immune blotting. For evaluation of effectiveness of rLip21 in ELISA test, 200 µg rLip21 with Montanide ISA70 adjuvant was injected subcutaneously in rabbits three times. Results showed that rLipL21 protein was highly expressed in 2YT media in presence of 0.1 mM IPTG after 16 hours incubation at 37 oC. Recombinant protein was purified 36 mg per liter using affinity batch formation method by Ni-NTA resin. ELISA with micro plate coated with 250 ng rLipL21 protein demonstrated prominently differences between test and control groups (P<0.01). The rLipL21 protein produced large amounts of antibodies in the rabbit. The protein was also able to detect high levels of antibody in animals immunised with Leptospira vaccine. The rLipL21 might be a good candidate for diagnosis and evaluation of antibody levels against Leptospira.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135556485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Arterial vascularisation of pig’s auditory tube with respect to a. palatina ascendens – a corrosion cast and morphometric study 猪听管的动脉血管化——一种腐蚀铸型和形态计量学研究

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI: 10.15547/bjvm.2366

N. Tsandev

{"title":"Arterial vascularisation of pig’s auditory tube with respect to a. palatina ascendens – a corrosion cast and morphometric study","authors":"N. Tsandev","doi":"10.15547/bjvm.2366","DOIUrl":"https://doi.org/10.15547/bjvm.2366","url":null,"abstract":"The aim of this study was to determine species-specific features of arterial vascularisation of the auditory tube of domestic pigs as a segment connecting the nasopharynx and the middle ear with regard to the increasing role of pigs as most appropriate model of human biomedical research. The arterial branches involved in the vascularisation of the auditory tube were described on corrosion casts. It was found out that in the direction of the middle ear, the tube received blood from branches of a. palatina ascendens, a. meningea media, a. temporalis profunda caudalis and rete mirabile epidurale rostrale, out of which the first of enumerated arteries was the most involved. In the majority of cases (87.5%) it gave off two branches that ramified in the lateral and medial walls of the tube. For the first time, it was demonstrated that a. palatina ascendens was separated from the facial artery and not from a. lingualis. The measurements on casts showed that the diameter of left a. palatina ascendens was statistically significantly larger than right one in both sexes, and that average diameters of either right or left arteries were larger in males. The results allowed concluding that a. palatina ascendens was predominantly involved in the arterial blood supply of porcine auditory tube.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"145 16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87515700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0