Brazilian Journal of Plant Physiology最新文献_第5页

Purification and characterization of cytosolic and cell wall β-galactosidases from Vigna unguiculata stems 荆芥茎中胞浆和细胞壁β-半乳糖苷酶的纯化及特性研究

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000100003

Fabrício Bonfim Sudério, Gislainy Karla da Costa Barbosa, E. Gomes-Filho, J. Enéas-Filho

{"title":"Purification and characterization of cytosolic and cell wall β-galactosidases from Vigna unguiculata stems","authors":"Fabrício Bonfim Sudério, Gislainy Karla da Costa Barbosa, E. Gomes-Filho, J. Enéas-Filho","doi":"10.1590/S1677-04202011000100003","DOIUrl":"https://doi.org/10.1590/S1677-04202011000100003","url":null,"abstract":"Three β-galactosidase isoforms, β-gal I and β-gal II (cytosolic) and β-gal III (cell wall-associated), were isolated from stems of Vigna unguiculata (L.) Walp. cv. Pitiuba seedlings. Purification consisted of aμMonia sulfate fractionation followed by chromatography in DEAE-Sephadex and Lactosyl-Sepharose columns. The two cytosolic isoforms showed the same chromatography pattern, which differed from that of β-gal III. Electrophoresis revealed a single band of protein for β-gal II and β-gal III which also expressed β-galactosidase activity in gel. The apparent molecular mass of the β-gal I, II and III was 89, 146 and 124 kDa, respectively. The three isoforms revealed the same optimal pH (4.0) and the same optimal assay temperature (55oC) for enzyme activity. The three isoforms were stable at temperatures up to 50oC, and incubation with glucose and galactose expanded their thermal stability as well as inhibited their activities. Galactose was the most effective in promoting these effects and β-gal I and II were competitively inhibited by this sugar. Kinetic analysis using β-PNPG as substrate, revealed KM of 1.69, 1.76 and 1.43 for β-gal I, β-gal II and β-gal III, respectively. The β-gal I was able to hydrolyze all synthetic substrates tested, whereas β-gal II exhibited only β-fucosidase and α-arabinosidase activities, and β-gal III was limited to the α-galactosidase, β-fucosidase and α-arabinosidase activities. These results are consistent with three distinct β-galactosidases exhibiting quite similar kinetic features, but endowed with different functional specificities probably related to their specific roles in the plant cell physiology.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88565210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

In memoriam to Alvim and Maestri 纪念阿尔维姆和马埃斯特里

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000100001

R. Bressan-Smith, A. Façanha

引用次数: 0

Ecophysiological studies of two andean forest contrasting species Abatia parviflora and Myrcianthes leucoxyla under Bogotá conditions, Colombia 哥伦比亚波哥大<e:1>条件下两种安第斯森林对比种小檗和月桂的生态生理研究

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000400008

Sara L. Colmenares-Trejos, L. Melgarejo, A. Romero

{"title":"Ecophysiological studies of two andean forest contrasting species Abatia parviflora and Myrcianthes leucoxyla under Bogotá conditions, Colombia","authors":"Sara L. Colmenares-Trejos, L. Melgarejo, A. Romero","doi":"10.1590/S1677-04202011000400008","DOIUrl":"https://doi.org/10.1590/S1677-04202011000400008","url":null,"abstract":"The physiological response of high Andean forest contrasting plants, Myrcianthes leucoxyla, a shadow-facultative heliophyte, and Abatia parviflora, a heliophyte, was studied during the transition from dry season to wet season in Bogota. Light response curves (A-PAR), water potential (Ψw), transpiration rate (E), stomatal conductance (gs), leaf soluble protein content and peroxidase activity were measured in this study in a 48h cycle. The values for E, gs, Ψw and Ψw predawn for M. leucoxyla were 0.07 to 4.89 µg/cm s, 0.02 to 0.5 cm s-1, -0.025 to -1.05 MPa, and-0.1 MPa, respectively. For A. parviflora E, gs, Ψw and Ψw predawn were 0.47 to 12.27 µg/cm s, 0.17 to 1.42 cm s-1, -0.05 to -0.3 MPa and -0.075 MPa, respectively. A. parviflora presented a tendency of stomatal closure at midday and peroxidase activity increased with the increasing solar radiation, this did not occur in M. leucoxyla. The light compensation point (Ic) in A. parviflora was about 18.10 µmol photon m-2s-1, typical for a heliophyte plant; while for M. leucoxyla it was 8.87 µmol photon m-2s-1, slightly above the maximal reported values for shadow plants and under the minimal reported values for light plants. The lowest photosynthetic rate (A) of M. leucoxyla (2.8 µmol CO2 m-2s-1) in comparison with A. parviflora (7.4 µmol CO2 m-2s-1) is compensated with a better photonic use efficiency (± 0.7792 mol C mol-1).","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77148774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Differential activation of the enzymatic antioxidant system of Abelmoschus esculentus L. under CdCl2 and HgCl2 exposure CdCl2和HgCl2对沙鼠酶抗氧化系统激活的影响

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000100007

A. Hameed, T. N. Qadri, Mahmooduzzafar, T. O. Siddiqi, M. Iqbal

{"title":"Differential activation of the enzymatic antioxidant system of Abelmoschus esculentus L. under CdCl2 and HgCl2 exposure","authors":"A. Hameed, T. N. Qadri, Mahmooduzzafar, T. O. Siddiqi, M. Iqbal","doi":"10.1590/S1677-04202011000100007","DOIUrl":"https://doi.org/10.1590/S1677-04202011000100007","url":null,"abstract":"Cadmium and Mercury induced varying responses in Abelmoschus esculentus L. in relation to enzymes (ascorbate peroxidase (APX, 1.11.1.11), catalase (CAT, 1.11.1.6), glutathione reductase (GR, 1.6.4.2) and superoxide dismutase (SOD, 1.15.1.1) which are most related to the levels of Hg and Cd applied and concentrations of thiol groups already present or induced upon treatment. In the present investigation varying concentrations of CdCl2 and HgCl2 (0, 0.05, 0.10, 0.50, 1 and 2mM respectively) applied to plant in the soil shows a significant increase in ascorbate peroxidase, glutathione reductase and superoxide dismutase activities, and the respective metal accumulation. It reveals a mechanism for constant detoxification of H2O2 that have to be associated with adaptations and ultimate survival of this plant species during stress conditions. A reduction of catalase activities was observed on exposure to these metals, which is supposedly due to the inhibition of enzyme synthesis. Root length, shoot length, number of leaves showed an enhancement with 0.05 mM CdCl2 dose then a gradual decline with the increase in concentrations. The results indicate that A. esculentus is tolerant to high concentrations of these metals, a property related to a differential activation of its enzymatic antioxidant system, and also reveal that this species has a higher capacity of Cd absorption.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72872102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Physic nut plants present high mycorrhizal dependency under conditions of low phosphate availability 物理坚果植物在低磷酸盐利用率条件下表现出高度的菌根依赖性

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000100006

E. L. Balota, Oswaldo Machineski, Priscila Viviane Truber, A. Scherer, F. S. D. Souza

{"title":"Physic nut plants present high mycorrhizal dependency under conditions of low phosphate availability","authors":"E. L. Balota, Oswaldo Machineski, Priscila Viviane Truber, A. Scherer, F. S. D. Souza","doi":"10.1590/S1677-04202011000100006","DOIUrl":"https://doi.org/10.1590/S1677-04202011000100006","url":null,"abstract":"The physic nut (Jatropha curcas L.) is a perennial tree that occurs naturally in the tropical and subtropical regions of Brazil. Fruits of physic nut present an oil content of 28% on a dry weight basis. Although the plant has adapted to diverse soil conditions such as low fertility, the correction of soil acidity and the addition of fertilizer are essential for highly productive plants. Thus, the response of the physic nut to different soil phosphorus levels (P) and arbuscular mycorrhizal fungi (AMF) inoculation must be characterized. Hence, the objective of the present study was to evaluate the response of physic nut seedlings to arbuscular mycorrhizal fungi (AMF) inoculation at different levels of soil P. Experiment was carried out in a greenhouse encompassing AMF treatments (inoculation with Gigaspora margarita or Glomus clarum, and the non inoculated controls), and phosphorus treatments (0, 25, 50, 100, 200 and 400 mg kg-1 added to soil). At low soil P levels, arbuscular mycorrhizal fungi inoculation had a significant positive effect on plant growth, shoot and root dry matter content, plant height, number of leaves, total leaf area, leaf area per leaf and the Dickson quality index. The root:shoot ratio and the leaf area ratio were also affected by mycorrhizal inoculation and the level of P addition. Physic nut plants exhibited high mycorrhizal dependency at soil P additions up to 50 mg kg-1.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75228761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Synergistic response of auxin and ethylene on physiology of Jatropha curcas L. 生长素和乙烯对麻疯树生理的协同响应。

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000100009

G. Joshi, A. Shukla, A. Shukla

{"title":"Synergistic response of auxin and ethylene on physiology of Jatropha curcas L.","authors":"G. Joshi, A. Shukla, A. Shukla","doi":"10.1590/S1677-04202011000100009","DOIUrl":"https://doi.org/10.1590/S1677-04202011000100009","url":null,"abstract":"Jatropha curcas L. is a versatile crop since all its plant material is brought to use either as energy source, industrial or medicinal purpose. Several studies are ongoing in different parts of the world to optimize, enhance and exploit the growth, fruiting cycle and different developmental stages of the plant so that the economic yield of the plants can be utilized to the fullest limits. Foliar application of plant growth regulators such as ethrel (an ethylene releasing compound), indole acetic acid (IAA) and naphthalene acetic acid (NAA) at 50, 100 and 150 ppm, was found to influence different morpho-physiological characters in Jatropha curcas L, such as plant height, collar diameter, tree spread, flower initiation, number of inflorescence per plant, number of male and female flowers per inflorescence, and the ratio of male: female flowers per inflorescence. Moreover, the leaves chlorophyll content, Fv/Fm value, nitrate reductase activity and proline content were also affected by synergistic response of auxin and ethylene. As higher the plant growth regulators concentration higher was the synergic effect on the Jatropha curcas L physiology.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84234740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Cadmium-induced oxidative stress and antioxidative enzyme response in water hyacinth and salvinia 镉诱导的水葫芦和鼠尾草氧化应激及抗氧化酶反应

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000200005

S. Vestena, J. Cambraia, C. Ribeiro, J. Oliveira, M. Oliva

引用次数: 59

Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant 磷和氮的相互作用:pdr23突变体在磷或氮限制下预计会失去QC身份

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000300006

Cibele Tesser da Costa, M. L. Strieder, S. Abel, C. A. Delatorre

{"title":"Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant","authors":"Cibele Tesser da Costa, M. L. Strieder, S. Abel, C. A. Delatorre","doi":"10.1590/S1677-04202011000300006","DOIUrl":"https://doi.org/10.1590/S1677-04202011000300006","url":null,"abstract":"Changes in root architecture are an important adaptive strategy used by plants in response to limited nutrient availability to increase the odds of acquiring them. The quiescent center (QC) plays an important role by altering the meristem activity causing differentiation and therefore, inducing a determinate growth program. The arabidopsis mutant pdr23 presents primary short root in the presence of nitrate and is inefficient in the use of nucleic acids as a source of phosphorus. In this study the effect of the pdr23 mutation on the QC maintenance under low phosphorus (P) and/or nitrogen is evaluated. QC identity is maintained in wild-type in the absence of nitrate and/or phosphate if nucleic acids can be used as an alternative source of these nutrients, but not in pdr23. The mutant is not able to use nucleic acids efficiently for substitute Pi, determinate growth is observed, similar to wild-type in the total absence of P. In the absence of N pdr23 loses the expression of QC identity marker earlier than wild-type, indicating that not only the response to P is altered, but also to N. The data suggest that the mutation affects a gene involved either in the crosstalk between these nutrients or in a pathway shared by both nutrients limitation response. Moreover loss of QC identity is also observed in wild-type in the absence of N at longer limitation. Less drastic symptoms are observed in lateral roots of both genotypes.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87920564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The master and the pupil: two close friends, two great plant physiologists 老师和学生，两个亲密的朋友，两个伟大的植物生理学家

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000100002

R. Barros

引用次数: 0

Chlorophyll a fluorescence as indicative of the salt stress on Brassica napus L. 甘蓝型油菜盐胁迫的叶绿素a荧光指示。

Brazilian Journal of Plant Physiology Pub Date : 2011-01-01 DOI: 10.1590/S1677-04202011000400001

M. A. Bacarin, S. Deuner, F. V. D. Silva, D. Cassol, Diolina Moura Silva

引用次数: 33