Brazilian Journal of Plant Physiology最新文献_第3页

Effects of hypoxia storage on gene transcript accumulation during tomato fruit ripening 低氧贮藏对番茄果实成熟过程中基因转录物积累的影响

Brazilian Journal of Plant Physiology Pub Date : 2012-06-01 DOI: 10.1590/S1677-04202012000200007

C. Pegoraro, Railson Schreinert dos Santos, M. Kruger, Aline Tiecher, L. C. Maia, C. Rombaldi, A. Oliveira

引用次数: 7

Oxidative processes during 'Golden' papaya fruit ripening “金色”木瓜果实成熟过程中的氧化过程

Brazilian Journal of Plant Physiology Pub Date : 2012-06-01 DOI: 10.1590/S1677-04202012000200002

E. Resende, P. F. Martins, R. Azevedo, A. P. Jacomino, Ilana Urbano Bron

{"title":"Oxidative processes during 'Golden' papaya fruit ripening","authors":"E. Resende, P. F. Martins, R. Azevedo, A. P. Jacomino, Ilana Urbano Bron","doi":"10.1590/S1677-04202012000200002","DOIUrl":"https://doi.org/10.1590/S1677-04202012000200002","url":null,"abstract":"'Golden' papayas at maturity stage 1 (15% yellow skin) were chosen to study selected oxidative processes, the activity of antioxidant enzymes and lipid peroxidation in storage at 22°C, during the ripening of the fruit. An increase in ethylene production was observed on the second day of storage and it was followed by an increase in respiration. An increased activity of catalase, glutathione reductase and ascorbate peroxidase was observed concurrently or soon after this increase in ethylene production and respiration. The increased activity of these enzymes near the peaks of ethylene production and respiration is related to the production of oxidants accompanying the onset of ripening. On the fourth day of storage, there was an increased lipid peroxidation and decreased activities of catalase, glutathione reductase and superoxide dismutase. Lipid peroxidation induces the increase of antioxidant enzymes, which can be verified by further increases in the activities of catalase, glutathione reductase, superoxide dismutase and ascorbate peroxidase. Unlike the other antioxidant enzymes, the ascorbate peroxidase activity in the pulp increased continuously during ripening, suggesting its important role in combating reactive oxygen species during papaya ripening. With regard to physical-chemical characteristics, the soluble solids did not vary significantly, the acidity and ascorbic acid contents increased, and hue angle and firmness decreased during storage. The results revealed that there was variation in the activity of antioxidant enzymes, with peaks of lipid peroxidation during the ripening of 'Golden' papaya. These results provide a basis for future research, especially with regard to the relationships among the climacteric stage, the activation of antioxidant enzymes and the role of ascorbate peroxidase in papaya ripening.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90253699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Physiological response of oil palm interspecific hybrids (Elaeis oleifera H.B.K. Cortes versus Elaeis guineensis Jacq.) to water deficit 油棕种间杂种(Elaeis oleifera H.B.K. Cortes vs . Elaeis guineensis Jacq.)对水分亏缺的生理反应

Brazilian Journal of Plant Physiology Pub Date : 2012-01-05 DOI: 10.1590/S1677-04202012000400006

Y. Méndez, L. M. Chacón, C. Bayona, H. Romero

{"title":"Physiological response of oil palm interspecific hybrids (Elaeis oleifera H.B.K. Cortes versus Elaeis guineensis Jacq.) to water deficit","authors":"Y. Méndez, L. M. Chacón, C. Bayona, H. Romero","doi":"10.1590/S1677-04202012000400006","DOIUrl":"https://doi.org/10.1590/S1677-04202012000400006","url":null,"abstract":"Water supply is the main yield-limiting abiotic factor of oil palm in Colombia. To determine the effect of water deficit on the physiology of oil palm and to assess the tolerance degree to this condition, interspecific Elaeis oleifera versus Elaeis guineensis 'U1273', 'U1859', 'U1914', and 'U1937' hybrids were planted for 60 days in soil with different water potentials (-0.042, -0.5, -1.0, and -2.0 MPa) in a complete randomized factorial design with three replications. The water potential, osmotic adjustment molecules (total and reducing sugar content), gas exchange (photosynthesis rate, stomatal conductance, water use efficiency, and leaf respiration), and vegetative growth (height, bulb diameter, leaf area, number of leaves, total dry matter, and assimilate partitioning between roots and aerial parts) were measured. Soil water potential had a significant effect on the ecophysiological response of the hybrids. At -2 MPa, there was practically no carbon dioxide assimilation. Under optimal moisture conditions, the 'U1859' hybrid recorded the highest photosynthetic rate and the lowest respiratory rate, while under moderate and severe water deficits, the 'U1937' hybrid showed the highest photosynthetic rate, the lowest respiratory rate, moved its assimilates mainly towards the roots, and was the only one to adjust its water potential (active accumulation of sugars). Two environment/genotype tolerance relationships were established based on high photosynthetic rate, low leaf respiration rate, water potential adjustment, water use efficiency under adequate moisture conditions ('U1859'>'U1937'='U1914'>'U1273'), and under water deficit ('U1937'>'U1914'>'U1859'>'U1273').","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88416396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Photosynthesis and antioxidant activity in Jatropha curcas L. under salt stress. 盐胁迫下麻疯树光合作用及抗氧化活性研究

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100008

M. Campos, B. S. D. Hsie, J. A. A. Granja, R. M. Correia, J. Almeida-Cortez, M. Pompelli

{"title":"Photosynthesis and antioxidant activity in Jatropha curcas L. under salt stress.","authors":"M. Campos, B. S. D. Hsie, J. A. A. Granja, R. M. Correia, J. Almeida-Cortez, M. Pompelli","doi":"10.1590/S1677-04202012000100008","DOIUrl":"https://doi.org/10.1590/S1677-04202012000100008","url":null,"abstract":"Biodiesel is an alternative to petroleum diesel fuel. It is a renewable, biodegradable, and nontoxic biofuel. Interest in the production of biodiesel from Jatropha curcas L. seeds has increased in recent years, but the ability of J. curcas to grow in salt-prone areas, such as the Caatinga semiarid region, has received considerably meager attention. The aim of this study was to identify the main physiological processes that can elucidate the pattern of responses of J. curcas irrigated with saline water, which commonly occurs in the semiarid Caatinga region. This study measured the activity of the antioxidant enzymes involved in the scavenging of reactive oxygen species, which include catalase (CAT) and ascorbate peroxidase (APX), as well as malondialdehyde (MDA) levels. The levels of chlorophyll (Chl), carotenoids, amino acids, proline, and soluble proteins were also analyzed. The net carbon assimilation rate (PN), stomata conductance (gs), and transpiration rate (E) decreased with salt stress. The activities of CAT and APX were decreased, while H2O2 and MDA levels as well as electrolyte leakage were significantly increased in salt-stressed plants compared to the untreated ones. These observations suggest that the ability of J. curcas plants resist to salt stress is associated with the activities of protective enzymes and their defensive functions. However, our results indicate that the reactive oxygen species scavenging system is not sufficient to protect J. curcas leaves against oxidative damage caused by salt stress, and, therefore, it cannot be treated as a salt tolerant plant species.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85951588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Moderate warm temperature improves shoot growth, affects carbohydrate status and stimulates photosynthesis of sweet orange plants 适度温暖的温度可以促进甜橙植株的茎部生长，影响碳水化合物状态，促进甜橙植株的光合作用

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100006

R. V. Ribeiro, E. Machado, Erick Espinoza-Núñez, R. Ramos, D. Machado

{"title":"Moderate warm temperature improves shoot growth, affects carbohydrate status and stimulates photosynthesis of sweet orange plants","authors":"R. V. Ribeiro, E. Machado, Erick Espinoza-Núñez, R. Ramos, D. Machado","doi":"10.1590/S1677-04202012000100006","DOIUrl":"https://doi.org/10.1590/S1677-04202012000100006","url":null,"abstract":"Citrus plants were grown under two thermal conditions for evaluating carbon metabolism acclimation to moderate warm temperature (30/20oC, day/night), and its likely impact on plant growth. As reference, plants were grown at 25/20oC, in which they were subjected to optimum temperature for photosynthesis during the diurnal period (25oC). Higher photosynthetic rates were found at 30/20oC as compared to 25/20oC in both mature and young leaves, being this response associated with higher stomatal conductance. After 30 days of thermal treatment, plants grown at 30/20oC presented higher shoot growth as compared to those at 25/20oC. The carbohydrate concentration decreased in stem and root tissues, while it increased in leaf tissues under moderate warm conditions. Both mature and young leaves showed higher photoassimilate consumption/exportation at 30/20oC than at 25/20oC. In this paper, we have proven that citrus plants present a positive balance in carbon metabolism as an acclimation mechanism to temperature changes, with plants presenting increased photosynthesis. Such photosynthetic acclimation was associated with improved vegetative growth, being both mature and young tissues sensitive to changes in thermal regimen.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81308619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Isolation and in silico characterization of cDNA encoding cyclophilin from etiolated Vigna mungo seedlings 黄化芒哥幼苗亲环蛋白cDNA的分离及硅质特性分析

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100009

Kalika Kuhar, Varun Gupta, R. Kansal, Vijay K. Gupta

引用次数: 1

Supplemental Ca2+ does not improve growth but it affects nutrient uptake in NaCl-stressed cowpea plants 添加Ca2+对nacl胁迫下豇豆植株的生长无促进作用，但对其养分吸收有影响

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100003

Francisco Valderez Augusto Guimarães, C. Lacerda, E. Marques, Carlos Eduardo Braga de Abreu, B. F. Aquino, J. T. Prisco, E. Gomes-Filho

引用次数: 12

Effectivity of anti-oxidative enzymatic system on diminishing the oxidative stress induced by aluminium in chickpea (Cicer arietinum L.) seedlings 抗氧化酶系统对鹰嘴豆(Cicer arietinum L.)幼苗铝氧化应激的影响

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100007

Sushant Singh, Anil Verma, V. Dubey

引用次数: 23

cDNA-AFLP analysis of Psidium guajava L. cultivars under water stress and mechanical injury: methodological implications 水分胁迫和机械损伤下番石榴栽培品种的dna - aflp分析:方法学意义

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100005

C. Furlan, S. Zanotta, A. Salatino

{"title":"cDNA-AFLP analysis of Psidium guajava L. cultivars under water stress and mechanical injury: methodological implications","authors":"C. Furlan, S. Zanotta, A. Salatino","doi":"10.1590/S1677-04202012000100005","DOIUrl":"https://doi.org/10.1590/S1677-04202012000100005","url":null,"abstract":"Studies involving amplified fragment length polymorphism (cDNA-AFLP) have often used polyacrylamide gels with radiolabeled primers in order to establish best primer combinations, to analyze, and to recover transcript-derived fragments. Use of automatic sequencer to establish best primer combinations is convenient, because it saves time, reduces costs and risks of contamination with radioactive material and acrylamide, and allows objective band-matching and more precise evaluation of transcript-derived fragments intensities. This study aimed at examining the gene expression of commercial cultivars of P. guajava subjected to water and mechanical injury stresses, combining analyses by automatic sequencer and fluorescent kits for polyacrylamide gel electrophoresis. Firstly, 64 combinations of EcoRI and MseI primers were tested. Ten combinations with higher number of polymorphic fragments were then selected for transcript-derived fragments recovering and cluster analysis, involving 45 saplings of P. guajava. Two groups were obtained, one composed by the control samplings, and another formed by samplings undergoing stress, with no clear distinction between stress treatments. The results revealed the convenience of using a combination of automatic sequencer and fluorescent kits for polyacrylamide gel electrophoreses to examine gene expression profiles. The Unweighted Pair Group Method with Arithmetic Mean analysis using Euclidean distances points out a similar induced response mechanism of P. guajava undergoing water stress and mechanical injury.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80632994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Foliar nitrogen and changes in free amino acid composition of Solanum lycopersicum under cadmium toxicity: kinetics of 15NH4+ 15NH4+镉胁迫下番茄茄叶片氮素及游离氨基酸组成的变化

Brazilian Journal of Plant Physiology Pub Date : 2012-01-01 DOI: 10.1590/S1677-04202012000100004

C. C. Haouari, A. Nasraoui, E. Carrayol, Maud Lelendais, M. Ghorbel, H. Gouia

{"title":"Foliar nitrogen and changes in free amino acid composition of Solanum lycopersicum under cadmium toxicity: kinetics of 15NH4+","authors":"C. C. Haouari, A. Nasraoui, E. Carrayol, Maud Lelendais, M. Ghorbel, H. Gouia","doi":"10.1590/S1677-04202012000100004","DOIUrl":"https://doi.org/10.1590/S1677-04202012000100004","url":null,"abstract":"Glutamate metabolism and amino acid translocation were investigated in the control and cadmium stressed shoots of tomato (Solanum lycopersicum - 63/5 F1), using (15N) ammonium and (15N) glutamate tracers. Regardless of organ type, [15N] ammonium assimilation occurred via glutamine synthetase (EC 6.3.1.2), in the control and stressed plants, and it did not depend on glutamate dehydrogenase (EC 1.4.1.2). The [15N] ammonium and ammonium accumulation patterns support the role of glutamate dehydrogenase in the deamination of [15N] glutamate to provide 2-oxoglutarate and [15N] ammonium. In the presence of cadmium, excess [15N] ammonium was incorporated into asparagine, which served as an additional detoxification molecule. In the presence both of methionine sulfoximine and cadmium, glutamate, alanine, and γ-amino butyrate of leaf tissue continued to become labelled with 15N. The labelling kinetics of amino acids in leaves of tomato plants in the presence of cadmium show that continued assimilation of [15N] ammonium can occur when the glutamine synthetase-glutamate synthase cycle is inhibited. The data provided evidence that the glutamine synthetase pathway and glutamate dehydrogenase play distinct roles in the source-sink nitrogen cycle of tomato leaves under cadmium stress conditions.","PeriodicalId":9278,"journal":{"name":"Brazilian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78072486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2