Biotechnologia Acta最新文献

{"title":"ACTIVITY OF AMP DEAMINASE AND 5′-NUCLEOTIDASE IN THE CYTOSOLIC KIDNEY FRACTION OF RATS UNDER THE CONDITIONS OF DIFFERENT PROTEIN AND SUCROSE CONTENT IN A DIET","authors":"A. S. Boichuk","doi":"10.15407/biotech15.02.049","DOIUrl":"https://doi.org/10.15407/biotech15.02.049","url":null,"abstract":"ACTIVITY OF AMP DEAMINASE AND 5′-NUCLEOTIDASE IN THE CYTOSOLIC KIDNEY FRACTION OF RATS UNDER THE CONDITIONS OF DIFFERENT PROTEIN AND SUCROSE CONTENT IN A DIET","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45394257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF SYSTEMIC INFLAMMATORY RESPONSE SYNDROME ON THE DEVELOPMENT OF OXIDATIVE STRESS DURING SIMULATION OF CHRONIC ALCOHOL INTOXICATION IN RATS","authors":"A. Mykytenko","doi":"10.15407/biotech15.02.062","DOIUrl":"https://doi.org/10.15407/biotech15.02.062","url":null,"abstract":"The aim of our study was to analyze changes in the development of oxidative stress in the liver of rats with chronic alcohol intoxication against the background of systemic inflammatory response syndrome based on the study of catalase and superoxide dismutase activity, concentration of malonic dialdehyde, oxidatively modified proteins and sulfide anion and superoxide anion production. Methods. Experimental studies were performed on 12 male Wistar rats weighing 180‒220 g. Animals were divided into two groups: 1 ‒ control and 2 ‒ animals, on which we simulated alcoholic hepatitis and SIRS. The activity of catalase and superoxide dismutase (SOD), the concentration of malonic dialdehyde (MDA) , oxidatively modified proteins (OMP) sulfide anion and superoxide anion production were studied in the rat liver homogenate. The obtained results were subjected to statistical processing using the Mann-Whitney test. Results. Analyzing the development of oxidative stress in the liver of rats, on which we simulated the combined effects of SIRS and prolonged alcohol intoxication, we found that the activity of SOD increased by 1.72 times (P<0.05), and catalase decreased by 1.18 times (P<0.05) compared with the control group. The production of superoxide anion radical in the liver of rats increased 2.21 times (P<0.05) in the group of animals with combined exposure to bacterial LPS and alcohol intoxication compared to control. The concentration of MDA increased 2.25 times (P<0.05), and OMP by 9.5 times (P<0.05) compared with control group. The concentration of sulfide anion in the liver of rats under the conditions of modeling the combined effects of SIRS and alcohol intoxication decreased by 1.44 times (P <0.05) compared with the control. Conclusions. Modeling of alcohol intoxication against the background of systemic inflammatory response syndrome leads to oxidative damage to lipid and protein structures of the liver due to increased production of superoxide anion radical and imbalance of antiradical protection.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43663444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GLUTAMINE DEPRIVATION AFFECTS THE EXPRESSION OF GENES WHICH CONTROL PYRUVATE DEHYDROGENASE ACTIVITY: THE IMPACT OF ERN1 KNOCKDOWN","authors":"M. Sliusar","doi":"10.15407/biotech15.02.070","DOIUrl":"https://doi.org/10.15407/biotech15.02.070","url":null,"abstract":"The aim of the current investigation was to study the expression of genes encoded pyruvate dehydrogenase subunits (PDHA1, PDHB, PDHX, DLAT, and DLD) in U87 glioma cells in response to glutamine deprivation in U87 glioma cells in relation to knockdown of ERN1 for evaluation of a possible dependence of the expression of these important regulatory genes from glutamine supply and ERN1 signaling. Methods. The expression of PDHA1, PDHB, PDHX, DLAT, and DLD genes was studied by real-time qPCR in control U87 glioma cells (transfected by vector) and cells with knockdown of ERN1 (transfected by dnERN1) after exposure to glutamine deprivation condition. Total RNA was extracted from glioma cells using TRIZOL reagent. An RNA quantity as well as spectral characteristics was measured using NanoDrop One. For reverse transcription of mRNAs we used Thermo Scientific Verso cDNA Synthesis Kit (Germany). The values of mRNA expressions were normalized to the level of ACTB mRNA and represented as percent of control (100 %). Results. It was shown that the expression level of PDH1, PDHB, DLAT, and DLD genes was down-regulated in control glioma cells treated by glutamine deprivation. At the same time, ERN1 knockdown is suppressed the effect of glutamine deprivation on PDHB and DLD gene expressions in glioma cells, but did not change significantly the impact of glutamine deprivation on the expression of PDHA1, DLAT, and PDHX genes. Conclusions. The results of this investigation demonstrated that the expression of PDH1, PDHB, PDHX, DLAT, and DLD genes was significantly affected by exposure of U87 glioma cells under glutamine deprivation condition and that the effect of glutamine deprivation on the expression of most these genes was modified in cells with knockdown of ERN1, a major signaling pathway of the endoplasmic reticulum stress.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49033634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE ACQUISITION OF RESISTANCE IN HUMAN NON-SMALL LUNG ADENOCARCINOMA MOR CELLS IS ASSOCIATED WITH UP-REGULATION OF ADAPTOR PROTEIN RUK/CIN85 AND EPITHELIAL-TO-MESENCHYMAL TRANSITION (EMT)","authors":"Y. Raynich","doi":"10.15407/biotech15.02.064","DOIUrl":"https://doi.org/10.15407/biotech15.02.064","url":null,"abstract":"The aim of this study was to elucidate the regulatory role of Ruk/CIN85 in chemoresistance and EMT using human NSCLC MOR cells as a model. Methods. MOR (ECACC 84112312) cell line and drug-resistant cell line MOR/0.2R (ECACC 96042335) were cultured under standard conditions in DMEM medium. Knockdown of Ruk/CIN85 in MOR/0.2R cells was performed using shRNA lentiviral technology. Expression levels of Ruk/CIN85, vimentin and E-cadherin were estimated by RT-PCR. Results and Discussion. According to the results of qPCR, MOR/0.R cells showed an extremely higher level of Ruk/CIN85 mRNA expression, more than 10 times higher than the parental MOR cells. Preliminary data revealed that knockdown of Ruk/CIN85 in the MOR/0.2R cells led to significant decrease of their resistance to doxorubicin and development of epithelial phenotype. High content of RukCIN85 in doxorubicin-resistant (MOR/R) cells strongly correlate with their mesenchymal phenotype (high expression level of vimentin and low – E-cadherin), while its down-regulation is followed by restoration of expression values characteristic of parental MOR cells. Conclusions. In summary, high expression level of Ruk/CIN85 in doxorubicin-resistant MOR cells and the reversion of EMT-related transcriptome parameters and sensitivity to drug due to knockdown of adaptor protein in this subline suggests its involvement in regulation of EMT as well as cancer cells chemoresistance. Thus, the adaptor protein Ruk/CIN85 can be considered as a tissue-specific marker of carcinogenesis and perspective target for drug development.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49196865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC DETECTION OF ANTITHROMBITIC CALIX[4]ARENE IN BLOOD PLASMA OF ANIMALS","authors":"V. A. Didkivskyi","doi":"10.15407/biotech15.02.051","DOIUrl":"https://doi.org/10.15407/biotech15.02.051","url":null,"abstract":"Previously sodium salt of 5,11,17,23-bis (dihydroxyphosphoryl) methylcalix[4]arene (C-145) was shown to be promising antithrombotic agent. Aim. This work was focused on the development of the method for the direct detection of this substance in blood plasma and estimation of pharmacokinetics of this compound. Methods. C-145 was injected into the Wistar rat’s lateral tail vein and into rabbit’s marginal vein of the ear (12 mg/kg) or was administrated per-oral. The anticoagulant effects of C-145 in blood plasma were confirmed by activated partial thromboplastin time (APTT) test. HPLC was performed using Agilent 1100 series (Agilent, USA) on the phase cyano ZorbaxCN Column which parameters were L×I.D. 25 cm×4.6 mm. Results. The maximal antithrombotic effect after the intravenous or per-oral administration of C-145 was observed after 4-6 hours. In particular clotting time in APTT-test in these blood plasma samples was prolonged trice and more (120 s against 46 s in control). Normalization of blood clotting was achieved after 24 hours after the injection. To develop a method for direct C-145 detection in blood plasma we selected samples with maximal prolongation of clotting time. For accurate analysis of blood plasma samples proteins were saturated by 10 % trichloroacetic acid. After neutralization by NaHCO3 samples were prepared using 12-port vacuum unit for solid-phase extraction (Agilent, USA) with a Bond-Elut C18 cartridge. Samples that contained C-145 were eluted by 100% methanol for the HPLC analysis performed on the phase cyano ZorbaxCN Column equilibrated with an acetonitrile solution (ddH2O:AcCN 99:1). Elution was performed using a combined gradient of acetonitrile (100 %) and citrate buffer (0.1 M, pH 6.0). The elution zone of C-145 was detected on the 128th minute at 280 nm. Conclusion. Application of the developed methods allowed us to confirm the direct antithrombotic effect of calix[4]arene C-145 on blood of experimental animals during intravenous administration. Also HPLC technique enabled to detect this substance in blood plasma and most likely could be applied for other biological solutions and could be modified for the quantitative analysis in the pharmacokinetic studies as well.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43657804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHARACTERISTICS OF WOUND INFECTIONS AND METHODS OF THEIR TREATMENT USING PREPARATIONS OF BIOLOGICAL ORIGIN","authors":"A. Oliinyk","doi":"10.15407/biotech15.02.007","DOIUrl":"https://doi.org/10.15407/biotech15.02.007","url":null,"abstract":"Surgical wound infections are the most common patients’ complications in the postoperative period. In the modern clinic, they worsen the disease prognosis and remain the most important and acute health problem in all countries of the world. The aim of the work was to analyze current scientific data on the peculiarities of the pathogenesis of wound infections and types of their pathogens, as well as drugs of biological origin in the treatment of wound infections. The paper discusses in detail the problem of infection of wound injuries during surgery and domestic injuries of various kinds. The main pathogens of wound infections are considered. Specific pathogenicity factors for bacteria of the genera Staphylococcus, Pseudomonas, Enterobacteriaceae were analyzed. Based on the analysis of literature sources, a list of drugs of biotechnological origin that can be effectively used in combination therapy for the treatment and prevention of wound infections was determined. Conclusions. The result is the identification of those mechanisms of pathogenesis of wound infections that determine the effectiveness of the use of drugs of biological origin in this pathology treatment.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48102567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR BASIS OF THE DEVELOPMENT OF INSULIN RESISTANCE IN OBESE ADOLESCENT AND ADULT MEN","authors":"Y. Viletska","doi":"10.15407/biotech15.02.072","DOIUrl":"https://doi.org/10.15407/biotech15.02.072","url":null,"abstract":"The aim of this work was to study the association between the expression of glucose metabolism related genes and insulin resistance, which expression is changed in obese adolescents and adult men with and without insulin resistance, for better understanding the molecular basis of the development of obesity complications and evaluation of possible contribution of these genes in development of insulin resistance. Methods. The expression level of genes related to glucose metabolism and their regulations was studied by real-time qPCR in adipose tissue and blood cells using SYBRGreen Mix and specific for each mRNA forward and reverse primers. Total RNA was extracted using TRIZOL reagent. For reverse transcription of mRNAs we used Thermo Scientific Verso cDNA Synthesis Kit (Germany). The values of mRNA expressions were normalized to the level of ACTB mRNA and represented as percent of control (100 %). Results. It was shown that in obese patients with insulin resistance the expression level of IRS1 (insulin receptor substrate 1), HK2 (hexokinase 2), PFKFB2 (6-phosphofructokinase/fructose-2,6-bisphosphatase 2) and PFKFB3 as well as circadian factors CLOCK and ARNTL genes in subcutaneous adipose tissue is significantly decreased as compared to obese men with normal sensitivity to insulin. At the same time, the development of insulin resistance in obese patients leads to up-regulation of PFKFB4, PER1, HSPA6, ALDH1A3, COL5A1, TIMP1, TIMP2, SPARC, and VCAN gene expressions in subcutaneous adipose tissue. The expression level of IGF1 (insulin-like growth factor 1) and IGFBP5 (IGF binding protein 5) as well as ENO1 (enolase 1) and ENO2 is down-regulated in the blood of obese adolescent with insulin resistance, but IGFBP2 and IGFBP7 gene expressions are significantly increased in these patients. Conclusions. The results of this investigation provide evidence that the development of insulin resistance in obese patients is associated with gene specific changes in the expression of many very important regulatory genes, which are endoplasmic reticulum stress responsible.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49480895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EPITHELIAL-MESENCHYMAL TRANSITION IN MELANOMA PROGRESSION: THE CONTRIBUTION OF ADAPTOR PROTEIN RUK/CIN85","authors":"B. V. Zhuravel","doi":"10.15407/biotech15.02.074","DOIUrl":"https://doi.org/10.15407/biotech15.02.074","url":null,"abstract":"The purpose of this study was to test the hypothesis that Ruk/CIN85 overexpression/knockdown in melanoma cells may be involved in the regulation of EMT. Materials and methods. The mouse melanoma cell line B16-F10 and its sublines with up-/down-regulation of Ruk/CIN85 (generated early using lentiviral technology) were used as a model for research. Melanoma cells were cultured in the complete RPMI 1610 medium under standard conditions. Proliferative activity of the cells was estimated using the MTT-test, and cell migratory potential was studied by the wound-healing assay. The data obtained were analyzed with parametric Student`s t-test. Results were expressed as mean ± SEM and significance was set at P<0.05. Results and Discussion. Cutaneous melanoma genesis is a multi-step process initiated by the transformation of a normal melanocyte following an oncogenic insult. Due to the transcriptome and metabolome reprogramming in the course of EMT, transformed melanoma cells change their phenotype and acquire increased proliferative rate, cell motility, invasiveness, and metastatic potential. According to the data obtained, overexpression of Ruk/CIN85 in B16 mouse melanoma cells (subclones Up7 and Up21) led to an increase in their proliferative activity by 1,6 and 1.8 times, respectively, at 24th hour in comparison with control Mock cells . At the 48th hour, when the cells reached confluence, the cell viability of subclones did not differ from the control ones. No statistically significant changes in the proliferative activity of B16 cells with suppressed expression of the adaptor protein (subclone Down) were found. In accordance with previous data, B16 cells overexpressing Ruk/CIN85 were characterized by strongly increased motility rate (more than twofold for both Up7 and Up21 subclones compared to control Mock cells). At the same time, knockdown of Ruk/CIN85 in B16 cells resulted in a decrease in their migratory activity by about 30%. Conclusions. All findings obtained demonstrated that the malignancy traits of melanoma B16 cells are inversely modulated upon up- and down-changes in adaptor protein Ruk/CIN85 expression levels suggesting its possible role in the control of EMT.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48546376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LONG-TERM EFFECTS OF SHAM SURGERY ON PHAGOCYTE FUNCTIONS IN RATS","authors":"Zh. Oliynyk","doi":"10.15407/biotech15.02.037","DOIUrl":"https://doi.org/10.15407/biotech15.02.037","url":null,"abstract":"Animal models of inflammatory disorders, including those of the nervous system are commonly used to explore the pathophysiological role of immune cell response in disease triggering and course and to develop biotechnology products for therapeutic use. Modeling some of these disorders, particularly neurodegenerative diseases, implies surgical manipulations for the intracerebral introduction of disease-initiating substances (toxins, amyloids etc.). Design of these experiments involves the use of sham-operated animals as a control of non-specific intrinsic side-effects elicited by surgical manipulations per se, including local and systemic inflammation, where phagocytic cells are key participants. Short-term post-surgical immunomodulatory effects are widely reported. However, no study thus far has examined the long term effects of sham-surgery on phagocyte functions. The purpose of this study was to evaluate the effect of sham-surgery, commonly used for modeling neurodegenerative diseases, on phagocyte functions in the far terms after the surgical manipulations. Materials and Methods. Adult male Wistar rats were used in the study. Sham surgery consisted of stereotactic unilateral injection of saline solution into the median forebrain bundle (sham-operated 1, SO1) or directly into the substantia nigra (sham-operated 2, SO2). Before the placebo surgery, animals were anaesthetized using nembutal and ketamine/xylazine correspondingly. Functional characteristics (phagocytic activity, oxidative metabolism, CD80/86 and CD206 expression) of phagocytes (microglia, peritoneal macrophages, circulating monocytes and granulocytes) were examined by flow cytometry. Differential leukocyte count was conducted using hematological analyzer. Results. Phagocytes from animals underwent of different protocols of placebo surgery, demonstrated various patterns of functional changes on day 29 after the manipulations. In animals from SO1 group, we observed signs of residual neuroinflammation (pro-inflammatory shift of microglia functional profile) along with ongoing resolution of systemic inflammation (anti-inflammatory metabolic shift of circulating phagocytes and peritoneal macrophages). In rats from SO2 group, pro-inflammatory polarized activation of peritoneal phagocytes was registered along with anti-inflammatory shift in microglia and circulating phagocytes. Conclusions. Sham surgery influences functions of phagocytic cells of different locations even in the far terms after the manipulations. These effects can be considered as combined long-term consequences of surgical brain injury and the use of anesthetics. Our observations evidences, that sham associated non-specific immunomodulatory effects should always be taken into consideration in animal models of inflammatory central nervous system diseases.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45249506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADAPTOR PROTEIN RUK/CIN85 IS INVOLVED IN THE GLUCOSE METABOLISM REPROGRAMMING IN BREAST CANCER CELLS","authors":"M. I. Bekala","doi":"10.15407/biotech15.02.047","DOIUrl":"https://doi.org/10.15407/biotech15.02.047","url":null,"abstract":"Aim. This study aimed to investigate the changes in glucose metabolism in mouse 4T1 breast adenocarcinoma cells with different levels of Ruk/CIN85 expression. Methods. We used 4T1 cells with stable overexpression (subline RukUp) or knockdown (subline RukDown) of Ruk/CIN85, as well as corresponding vector control sublines Mock and Scr. Cells were cultured in the complete RPMI-1640 medium under standard conditions. mRNA expression levels were estimated by RT2-PCR, enzymes activities were measured by spectrophotometric and/or fluorometric assays. Results. Analysis of mRNA expression of glucose metabolism-related genes in RukUp and RukDown cells revealed that glycolysis genes are preferentially overexpressed in RukUp cells, and downregulated in RukDown cells. Thus, RukUp cells were characterized by significantly overexpressed Slc2a1, Gck, Aldoa, and Ldha, while in RukDown cells these genes were either down regulated or not changed. However, the expression of TCA (tricarboxylic acid) cycle enzyme Mdh2 increased dramatically (by 7,8 times) in RukDown cells. In detail, we observed statistically significant changes in the activity of all studied enzymes in RukUp cells (increase by 1,5-1,9 times for glycolysis enzymes and G6PD, and decrease by 1,33-1,69 times for TCA enzymes). However, in RukDown cells we did not find any significant changes in glycolysis enzymes activities, but activities of mitochondrial IDH3 and MDH2 were elevated by 1,65 and 1,59 times, respectively. Conclusions. The results obtained indicate that adaptor protein Ruk/CIN85 is involved in the metabolic reprogramming during breast cancer progression. High level of Ruk/CIN85 expression is associated with potentiation of the Warburg effect.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43790341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}