Biotechnologia Acta最新文献_第10页

OBTAINING OF PLANT TISSUE CULTURE Scutellaria baicalensis GEORGI. AND ITS BIOCHEMICAL ANALYSIS 植物组织培养黄芩的获得。及其生化分析

Biotechnologia Acta Pub Date : 2021-12-01 DOI: 10.15407/biotech14.06.0053

О. О. Poronnik

{"title":"OBTAINING OF PLANT TISSUE CULTURE Scutellaria baicalensis GEORGI. AND ITS BIOCHEMICAL ANALYSIS","authors":"О. О. Poronnik","doi":"10.15407/biotech14.06.0053","DOIUrl":"https://doi.org/10.15407/biotech14.06.0053","url":null,"abstract":"Aim. To obtain a plant tissue culture of S. baicalensis as a possible source of biologically active compounds (BAC) with a wide range of pharmacological action. Methods. Plant tissue culture, photocolorimetric method, reversed-phase high performance liquid chromatography (HPLC) method. Results. Two stably productive plant tissue culture strains (16SB3 and 20SB4) of S. baicalensis were obtained from fragments of roots seedling on a specially developed agar nutrient medium 5С01. The yield of dry biomass from 1 liter of this medium per passage (21st day of growth) for strain 16SB3 is 25–30 g, for strain 20SB4 – 30–40 g. The total content of flavonoids in dry biomass was in terms of routine for strains 16SB3 and 20SB4 – 0.6–0.9 and 0.7–0.9 mg/g, respectively, and the yield of flavonoids – 18–27 and 21–36 mg/l of nutrient medium, respectively. BAC typical for plants in nature, in particular, flavonoids vogonin, baikalein, neobaikalein, skulkapfavon and their derivatives, were found in the studied biomass of both strains. Conclusions. It was found that the biomass of the two strains of S. baicalensis plant tissue culture accumulated the same BAC, in particular, flavonoids, as do plants in natural conditions. The resulting plant tissue culture is promising as a possible source of Baikal skullcap BAC.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42396266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRANSFORMATION MEDIATED BY Agrobacterium rhizogenes AS APPROACH OF STIMULATING THE SYNTHESIS OF ANTIOXIDANT COMPOUNDS IN Artemisia absinthium L. 根际农杆菌介导转化促进苦艾抗氧化化合物合成的研究。

Biotechnologia Acta Pub Date : 2021-12-01 DOI: 10.15407/biotech14.06.071

A. Olkhovska

{"title":"TRANSFORMATION MEDIATED BY Agrobacterium rhizogenes AS APPROACH OF STIMULATING THE SYNTHESIS OF ANTIOXIDANT COMPOUNDS IN Artemisia absinthium L.","authors":"A. Olkhovska","doi":"10.15407/biotech14.06.071","DOIUrl":"https://doi.org/10.15407/biotech14.06.071","url":null,"abstract":"Artemisia absinthium L. plants are known as producers of substances with antioxidant properties. Among others, phenols and flavonoids are found in these plants. The synthesis of these bioactive compounds can be activated by genetic transformation. This process can be carried out even without the transfer of specific genes involved in the synthesis of flavonoids. Thus, “hairy” roots, obtained after Agrobacterium rhizogenes – mediated transformation, can produce a variety of valuable substances. The aim of the study was to obtaine A. absinthium “hairy” roots with high phenolic content. Methods. “Hairy” roots of plants were obtained by co-cultivation leaves with suspension of A. rhizogenes with pCB124 vector. The presence of transferred genes was confirmed by PCR. The reactions with AlCl3 and Folin-Ciocalteu reagent were used to determine the total flavonoids and phenols content. The antioxidant activity of extracts was evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Results. PCR analysis detected the presence of bacterial rol genes and the absence of рСВ124 plasmid genes. Root lines differed in growth rate. “Hairy” roots were characterized by a higher phenolic content, particularly flavonoids (up to 4.784 ± 0.10 mg/g FW) compared to control (3.861±0.13 mg/g FW). Also, extracts from transgenic roots demonstrated higher antioxidant activity in the reaction with DPPH reagent (EC50 = 3.657 mg) when compared with extracts from control plants (EC50 = 6,716 mg). Conclusions. Transformation of A. absinthium mediated by A. rhizogenes can be applied for obtaining transgenic root lines with increased phenolic content and higher antioxidant activity.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42057232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sinorhizobium meliloti AS A PERSPECTIVE OBJECT FOR MODERN BIOTECHNOLOGY 作为现代生物技术研究对象的中国根瘤菌

Biotechnologia Acta Pub Date : 2021-12-01 DOI: 10.15407/biotech14.06.023

G. G. Pirhanov

引用次数: 0

DEOXYRIBOZYMES IN DETECTION OF PATHOGENIC BACTERIA 脱氧核酶在致病菌检测中的应用

Biotechnologia Acta Pub Date : 2021-10-01 DOI: 10.15407/biotech14.05.005

K. A. P. Gaminda

引用次数: 1

PRACTICAL USE OF GOAT MILK AND COLOSTRUM 羊奶和初乳的实际应用

Biotechnologia Acta Pub Date : 2021-10-01 DOI: 10.15407/biotech14.05.038

I. Voloshyna

引用次数: 2

CIRCULATING TUMOR CELLS: WHERE WE LEFT OFF? 循环肿瘤细胞：我们在哪里停止？

Biotechnologia Acta Pub Date : 2021-08-01 DOI: 10.15407/biotech14.04.005

I. Kryvoshlyk

{"title":"CIRCULATING TUMOR CELLS: WHERE WE LEFT OFF?","authors":"I. Kryvoshlyk","doi":"10.15407/biotech14.04.005","DOIUrl":"https://doi.org/10.15407/biotech14.04.005","url":null,"abstract":"Cancer metastasis and recurrence are the leading causes of cancer-related death. Tumor cells which leave the primary or secondary tumors and shed into the bloodstream are called circulating tumor cells (CTC). These cells are the key drivers of cancer dissemination to surrounding tissues and to distant organs. The use of CTC in clinical practice necessitates the deep insight into their biology, as well as into their role in cancer evasion of immune surveillance, tumor resistance to chemo- radio- and immunotherapies and metastatic dormancy. Aim. The purpose of the work was to review the current knowledge on the CTC biology, as well as the prospects for their use for the diagnosis and targeted treatment of metastatic disease. Methods. The work proposed the integrative literature review using MEDLINE, Biological Abstracts and EMBASE databases. Results. This review summarizes and discusses historical milestones and current data concerning СTС biology, the main stages of their life cycle, their role in metastatic cascade, clinical prospects for their use as markers for the diagnosis and prognostication of the disease course, as well as targets for cancer treatment. Conclusions. Significant progress in the area of CTC biology and their use in cancer theranostics convincingly proved the attractiveness of these cells as targets for cancer prognosis and therapy. The effective use of liquid biopsy with quantitative and phenotypic characteristics of CTCs is impeded by the imperfection of the methodology for taking biological material and by the lack of reliable markers for assessing the metastatic potential of CTCs of various origins. The variety of mechanisms of tumor cells migration and invasion requires the development of complex therapeutic approaches for anti-metastatic therapy targeting CTCs. Efforts to address these key issues could help developing new and effective cancer treatment strategies.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45455883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L. 报告基因在尾芒属植物中的瞬时表达。

Biotechnologia Acta Pub Date : 2021-08-01 DOI: 10.15407/biotech14.04.053

O. Yaroshko

{"title":"TRANSIENT EXPRESSION OF REPORTER GENES IN CULTIVARS OF Amaranthus caudatus L.","authors":"O. Yaroshko","doi":"10.15407/biotech14.04.053","DOIUrl":"https://doi.org/10.15407/biotech14.04.053","url":null,"abstract":"Local cultivars of A. caudatus: Helios and Karmin were used as plant material. Amaranth is a new pseudocereal introduced in Ukraine. The plant biomass of amaranth is used in medicine, food industry and cosmetology industry. Aim. The purpose of the work was to identify the optimal conditions for the transient expression of reporter genes in Amaranthus caudatus cultivars. Methods. Biochemical and microscopy methods were used in the following work. Seedlings and adult plants of different age were infiltrated with agrobacterial suspensions separately (genetic vector pCBV19 with a uidA gene and genetic vector pNMD2501 with a gfp gene in Agrobacterium tumefaciens GV3101 strain). Results. Transient expression of the uidA and gfp genes was obtained in amaranth plants after conduction series of experiments. The most intensive transient expression of gfp and uidA genes was observed in seedlings infiltrated at the age of 1 day. The maximum fluorescence of the GFP protein was observed on 5th–6th days. Conclusions. It was shown that the cultivar Helios was more susceptible to agrobacterial infection than the cultivar Karmin. The effectiveness of Agrobacterium mediated transformation was from 16% to 95% for the Helios cultivar and from 12% to 93% for the Karmin cultivar. The obtained results indicate that the studied amaranth cultivars can potentially be used for obtaining transient expression of target genes and synthesizing target proteins in their tissues in the future.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44795303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BIOTECHNOLOGICAL RESEARCH IN THE CREATION AND PRODUCTION OF ANTIRABIC VACCINES 抗狂犬病疫苗研制中的生物技术研究

Biotechnologia Acta Pub Date : 2021-08-01 DOI: 10.15407/biotech14.04.028

Y. Krasnopolsky

{"title":"BIOTECHNOLOGICAL RESEARCH IN THE CREATION AND PRODUCTION OF ANTIRABIC VACCINES","authors":"Y. Krasnopolsky","doi":"10.15407/biotech14.04.028","DOIUrl":"https://doi.org/10.15407/biotech14.04.028","url":null,"abstract":"Rabies is a neurological disease of a viral nature, leading to death. Rabies virus is an RNA virus that invades the central nervous system, leading to neuronal dysfunction. Timely vaccination can prevent the diseases development. Aim. The article is devoted to immunobiotechnological research aimed at creating antirabic vaccines. Results. The history of the antirabic vaccines creation from the first inactivated vaccines obtained from nervous tissue to the cultivation of the virus on animal cell cultures is considered. The article presents commercially available anti-rabies vaccines: their composition, the used rabies virus strains, cell cultures, the methods of inactivation and purification. The technology of producing an anti-rabies vaccine based on a Pitman Moore virus strain and a chicken fibroblast cell culture is presented. The advantages of different vaccine types are considered: live attenuated, peptide, liposomal, RNA vaccines, vaccines based on viral vectors, transgenic plants and reverse genetics methods. Conclusions. The development of biotechnology, immunology and virology makes it possible to improve constantly vaccine preparations, including those against rabies, increasing their effectiveness and safety.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49484713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

TWO-STAGE DEGRADATION OF SOLID ORGANIC WASTE AND LIQUID FILTRATE 固体有机废物和液体滤液的两阶段降解

Biotechnologia Acta Pub Date : 2021-08-01 DOI: 10.15407/biotech14.04.070

V. Hovorukha

{"title":"TWO-STAGE DEGRADATION OF SOLID ORGANIC WASTE AND LIQUID FILTRATE","authors":"V. Hovorukha","doi":"10.15407/biotech14.04.070","DOIUrl":"https://doi.org/10.15407/biotech14.04.070","url":null,"abstract":"The accumulation of solid and liquid organic waste requires their treatment to develop energy biotechnologies and prevent environment pollution. Aim. The goal of the work was to study the efficiency of the purification of the filtrate from dissolved organic compounds by aerobic oxidation and methane fermentation. Methods. The standard methods were used to determine рН and redox potential (Eh), the gas composition, the content of short-chain fatty acids, the concentration of dissolved organic compounds counting to the total сarbon. The efficiency of two types of microbial metabolism for the degradation of soluble organic compounds of filtrate was compared. Results. The aerobic oxidation was established to provide 1.9 times more efficient removal of dissolved organic compounds, compared with the anaerobic methane fermentation. However, it provided CH4 yield 1 L/dm3 of filtrate (сarbon concentration — 1071 mg/L). The necessity to optimize the methods for purifying filtrate to increase the efficiency of the process was determined. Conclusions. The obtained results will be the basis to develop complex biotechnology providing not only the production of environmentally friendly energy H2 via the fermentation of solid food waste, but also the purification of filtrate to solve the ecological and energy (CH4 production) problem of society.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42461503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PROBLEMS OF SOAPSTOCK TREATMENT OF VEGETABLE OIL PRODUCTIONS AND THEIR SOLUTIONS 植物油生产中皂液处理存在的问题及对策

Biotechnologia Acta Pub Date : 2021-08-01 DOI: 10.15407/biotech14.04.080

L. Sabliy

{"title":"PROBLEMS OF SOAPSTOCK TREATMENT OF VEGETABLE OIL PRODUCTIONS AND THEIR SOLUTIONS","authors":"L. Sabliy","doi":"10.15407/biotech14.04.080","DOIUrl":"https://doi.org/10.15407/biotech14.04.080","url":null,"abstract":"Wastewater generated during vegetable oil production contains various pollutants that enter it during soapstock processing: fats and fatty acids and their salts (aqueous soap solutions), glycerin, phosphoglycerates, neutral fat, phosphatides, proteins, carbohydrates, dyes, unsaponifiable and waxy substances, salts, mechanical impurities, etc. Aim. The purpose of the work was to study the processes of purification of industrial wastewater from oil production and to propose an effective technology for their treatment, taking into account the regulatory requirements for the discharge of treated wastewater into the city sewage system. Methods. Chemical oxygen demand (COD) was determined by the dichromate method. The concentration of suspended solids was determined by gravimetric method. Results. As a result of research, calcium carbonate was chosen as an alkaline reagent. After treatment of soapstock with calcium carbonate followed by flotation, the effect of removing the suspended particles was 70–75%, and COD decreased by 60%. On the basis of the research, a technology for processing soapstock was proposed, including sequential processes of physicochemical wastewater treatment —averaging, alkalization with calcium carbonate, stage I of flotation, coagulation, stage II of flotation, oxidation with hydrogen peroxide, filtration through quartz filters and adsorption on carbon filters. Conclusion. An effective technology for preliminary cleaning of the soapstocks oil production has been developed. This will significantly reduce the concentration of organic matter and other pollutants in soapstocks, which will significantly reduce the impact of such effluents on the processes of biological wastewater treatment of urban wastewater treatment plants.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44133292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0