iScience notesPub Date : 2019-01-01Epub Date: 2019-10-10DOI: 10.22580/iscinotej4.4.3
Matthew Parent, Samuel T Hess
{"title":"QUANTIFICATION OF MITOCHONDRIAL MEMBRANE CURVATURE BY THREE-DIMENSIONAL LOCALIZATION MICROSCOPY.","authors":"Matthew Parent, Samuel T Hess","doi":"10.22580/iscinotej4.4.3","DOIUrl":"10.22580/iscinotej4.4.3","url":null,"abstract":"<p><p>Mitochondrial membrane organization is important for many biological functions, and is implicated in a number of diseases, but conventional microscopy has insufficient resolution to image biologically relevant structures. We present methods to quantify nanoscale membrane curvature using three-dimensional localization-based super-resolution microscopy. Localizations are analyzed using a cluster algorithm followed by principal component analysis to determine local membrane curvature. Results are shown for mitochondria in C2C12 mouse myotubes labeled with Tom20-Dendra2.</p>","PeriodicalId":92659,"journal":{"name":"iScience notes","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8992009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44488935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
iScience notesPub Date : 2018-12-28DOI: 10.22580/ISCINOTEJ3.3.4
A. Diaspro
{"title":"LIQUITOPY - Addressing the Design of a Liquid Microscopy","authors":"A. Diaspro","doi":"10.22580/ISCINOTEJ3.3.4","DOIUrl":"https://doi.org/10.22580/ISCINOTEJ3.3.4","url":null,"abstract":"","PeriodicalId":92659,"journal":{"name":"iScience notes","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45543685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
iScience notesPub Date : 2018-12-28DOI: 10.22580/2018/ISCINOTEJ3.3.3
Sanyukta Ghosh, R. Mallik
{"title":"Thermoelectric Power Generation- An Efficient Way of Waste Heat Recovery","authors":"Sanyukta Ghosh, R. Mallik","doi":"10.22580/2018/ISCINOTEJ3.3.3","DOIUrl":"https://doi.org/10.22580/2018/ISCINOTEJ3.3.3","url":null,"abstract":"","PeriodicalId":92659,"journal":{"name":"iScience notes","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45753106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
iScience notesPub Date : 2018-08-30DOI: 10.22580/ISCINOTEJ3.3.1
P. Mondal
{"title":"iLIFE - The Next Generation Imaging Cytometry: A Boon to Health-Care & Medicine","authors":"P. Mondal","doi":"10.22580/ISCINOTEJ3.3.1","DOIUrl":"https://doi.org/10.22580/ISCINOTEJ3.3.1","url":null,"abstract":"","PeriodicalId":92659,"journal":{"name":"iScience notes","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46506956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
iScience notesPub Date : 2017-01-01DOI: 10.22580/2016/iSciNoteJ2.2.1
Matthew Valles, Samuel T Hess
{"title":"A CROSS BEAM EXCITATION GEOMETRY FOR LOCALIZATION MICROSCOPY.","authors":"Matthew Valles, Samuel T Hess","doi":"10.22580/2016/iSciNoteJ2.2.1","DOIUrl":"https://doi.org/10.22580/2016/iSciNoteJ2.2.1","url":null,"abstract":"Fluorescence microscopy is popular for its noninvasive properties and its use in imaging multiple species simultaneously (1). Furthermore, superresolution fluorescence localization microscopy methods such as fluorescence photoactivation localization microscopy (FPALM) utilize photoactivatable, photoswitchable, and photoconvertible fluorescent proteins to improve the lateral resolution of conventional fluorescence microscopy by an order of magnitude (2-4). However, our understanding of the effects of excitation light polarization coupled to the transition dipole orientation of fluorescent labels within the sample is incomplete. Specifically, the excitation rate of a given label is proportional to cos2θ, where θ is the angle between the excitation illumination polarization and the transition dipole moment of the label(1). Because of this relationship between the laser polarization and transition dipole orientation, using widefield circularly-polarized illumination, one cannot excite all possible fluorophore orientations simultaneously with equal efficiency. Since thresholds are commonly used in localization microscopy during the stage of identification of fluorophores, the number of localized molecules can also be reduced by such polarization effects. Here, we briefly present an experimental excitation geometry called cross-beam, which uses two lasers with similar wavelength to excite labels with any transition dipole orientation.","PeriodicalId":92659,"journal":{"name":"iScience notes","volume":"2017 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428190/pdf/nihms-987301.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37262370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}