R. Lister, Alyssa A. Chamberlain, F. Einstein, Bingruo Wu, D. Zheng, Bin Zhou
{"title":"Intrauterine Programming of Diabetes Induced Cardiac Embryopathy.","authors":"R. Lister, Alyssa A. Chamberlain, F. Einstein, Bingruo Wu, D. Zheng, Bin Zhou","doi":"10.23880/doij-16000202","DOIUrl":"https://doi.org/10.23880/doij-16000202","url":null,"abstract":"Background Maternal hyperglycemia is a well-recognized risk factor for fetal congenital heart disease. However, the underlying cellular and molecular mechanisms are not well characterized. We hypothesize that maternal hyperglycemia leading to congenital heart are linked to abnormal DNA methylation and mRNA expression at cardiac specific loci. Methods Hyperglycemia was induced in normal 8-week old CD-1 female mice with a one-time intraperitoneal injection of 150 mg/kg of streptozotocin (STZ) 2 weeks prior to mating. Histological analysis of fetal cardiac morphology was evaluated for malformations on embryonic day (E) 16.5 of control pups and pups exposed to maternal hyperglycemia. We used a massively-parallel sequencing-based methylation sensitive restriction based assay to examine genome-wide cytosine methylation levels at >1.65 million loci in neonatal hearts on post-natal (P) day 0. Functional validation was performed with real time quantitative polymerase chain reaction (RT-qPCR). Results Cardiac structural defects occurred in 28% of the pups (n=12/45) of hyperglycemic dams versus 7% (n=4/61) of controls. Notable phenotypes were hypoplastic left or right ventricle, double outlet right ventricle, ventricular septal defect, and left ventricular outflow tract obstruction. A 10-fold increase in DNA methylation of gene promoter regions was seen in many cardiac important genes in the experimental versus control P0 neonates and have corresponding decreases in gene expression in 21/32 genes functionally validated. Conclusion Maternal hyperglycemia alters DNA methylation and mRNA expression of some cardiac genes during heart development. Quantitative, genome-wide assessment of cytosine methylation can be used as a discovery platform to gain insight into the mechanisms of hyperglycemia-induced cardiac anomalies.","PeriodicalId":92194,"journal":{"name":"Diabetes & obesity international journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68917557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Intrauterine Programming of Diabetes Induced Cardiac Embryopathy.","authors":"Rolanda Lister, Alyssa Chamberlain, Francine Einstein, Bingruo Wu, DeYou Zheng, Bin Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Maternal hyperglycemia is a well-recognized risk factor for fetal congenital heart disease. However, the underlying cellular and molecular mechanisms are not well characterized. We hypothesize that maternal hyperglycemia leading to congenital heart are linked to abnormal DNA methylation and mRNA expression at cardiac specific loci.</p><p><strong>Methods: </strong>Hyperglycemia was induced in normal 8-week old CD-1 female mice with a one-time intraperitoneal injection of 150 mg/kg of streptozotocin (STZ) 2 weeks prior to mating. Histological analysis of fetal cardiac morphology was evaluated for malformations on embryonic day (E) 16.5 of control pups and pups exposed to maternal hyperglycemia. We used a massively-parallel sequencing-based methylation sensitive restriction based assay to examine genome-wide cytosine methylation levels at >1.65 million loci in neonatal hearts on post-natal (P) day 0. Functional validation was performed with real time quantitative polymerase chain reaction (RT-qPCR).</p><p><strong>Results: </strong>Cardiac structural defects occurred in 28% of the pups (n=12/45) of hyperglycemic dams versus 7% (n=4/61) of controls. Notable phenotypes were hypoplastic left or right ventricle, double outlet right ventricle, ventricular septal defect, and left ventricular outflow tract obstruction. A 10-fold increase in DNA methylation of gene promoter regions was seen in many cardiac important genes in the experimental versus control P0 neonates and have corresponding decreases in gene expression in 21/32 genes functionally validated.</p><p><strong>Conclusion: </strong>Maternal hyperglycemia alters DNA methylation and mRNA expression of some cardiac genes during heart development. Quantitative, genome-wide assessment of cytosine methylation can be used as a discovery platform to gain insight into the mechanisms of hyperglycemia-induced cardiac anomalies.</p>","PeriodicalId":92194,"journal":{"name":"Diabetes & obesity international journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293196/pdf/nihms-1596152.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38043447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"It is Time to Obligate the Warning Messages on Junk Food Packages and Advertisements: A Step to live long.","authors":"Z. Ahmed, Y. Farjana","doi":"10.23880/DOIJ-16000149","DOIUrl":"https://doi.org/10.23880/DOIJ-16000149","url":null,"abstract":"Business is always profitable when it is done with our inalienable basic needsfood, clothing, shelter, education and healthcare. To survive and thrive in huge competition, every company uses several promotional tools. For successful marketing of a product, four promotional tools are typically usedadvertisement, sales promotion, public relation and direct marketing. The expenditure of food industry, whose marketing policy targets children and teenagers, is more than $1.6 billion per year for advertisement alone [1]. Nearly all foods featured in advertisements targeted towards young people are junk foods and several researches suggest that food advertising have substantial impact on dietary habit of the young, particularly on increased consumption of snacks, fast foods and sweetened beverages and hence elevated increase in body mass index (BMI) [1,2].","PeriodicalId":92194,"journal":{"name":"Diabetes & obesity international journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68918012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"It is Time to Obligate the Warning Messages on Junk Food Packages and Advertisements: A Step to live long.","authors":"Z Ahmed, Y Farjana","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":92194,"journal":{"name":"Diabetes & obesity international journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766035/pdf/nihms859085.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35736549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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