Proceedings. IEEE International Conference on Micro Electro Mechanical Systems最新文献

GEOMETRIC DETERMINANTS OF CELL VIABILITY FOR 3D-PRINTED HOLLOW MICRONEEDLE ARRAY-MEDIATED DELIVERY. 3d 印刷空心微针阵列介导递送的细胞活力几何决定因素。

Proceedings. IEEE International Conference on Micro Electro Mechanical Systems Pub Date : 2024-01-01 Epub Date: 2024-02-22 DOI: 10.1109/mems58180.2024.10439381

Sunandita Sarker, Jinghui Wang, Shrey A Shah, Christopher M Jewell, Kinneret Rand-Yadin, Miroslaw Janowski, Piotr Walczak, Yajie Liang, Ryan D Sochol

{"title":"GEOMETRIC DETERMINANTS OF CELL VIABILITY FOR 3D-PRINTED HOLLOW MICRONEEDLE ARRAY-MEDIATED DELIVERY.","authors":"Sunandita Sarker, Jinghui Wang, Shrey A Shah, Christopher M Jewell, Kinneret Rand-Yadin, Miroslaw Janowski, Piotr Walczak, Yajie Liang, Ryan D Sochol","doi":"10.1109/mems58180.2024.10439381","DOIUrl":"10.1109/mems58180.2024.10439381","url":null,"abstract":"A wide range of emerging biomedical applications and clinical interventions rely on the ability to deliver living cells via hollow, high-aspect-ratio microneedles. Recently, microneedle arrays (MNA) have gained increasing interest due to inherent benefits for drug delivery; however, studies exploring the potential to harness such advantages for cell delivery have been impeded due to the difficulties in manufacturing high-aspect-ratio MNAs suitable for delivering mammalian cells. To bypass these challenges, here we leverage and extend our previously reported hybrid additive manufacturing (or \"three-dimensional (3D) printing) strategy-i.e., the combined the \"Vat Photopolymerization (VPP)\" technique, \"Liquid Crystal Display (LCD)\" 3D printing with \"Two-Photon Direct Laser Writing (DLW)\"-to 3D print hollow MNAs that are suitable for cell delivery investigations. Specifically, we 3D printed four sets of 650 μm-tall MNAs corresponding to needle-specific inner diameters (IDs) of 25 μm, 50 μm, 75 μm, and 100 μm, and then examined the effects of these MNAs on the post-delivery viability of both dendritic cells (DCs) and HEK293 cells. Experimental results revealed that the 25 μm-ID case led to a statistically significant reduction in post-MNA-delivery cell viability for both cell types; however, MNAs with needle-specific IDs ≥ 50 μm were statistically indistinguishable from one another as well as conventional 32G single needles, thereby providing an important benchmark for MNA-mediated cell delivery.","PeriodicalId":91953,"journal":{"name":"Proceedings. IEEE International Conference on Micro Electro Mechanical Systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10932570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D-MICROPRINTED COAXIAL NOZZLE FOR FABRICATING LONG, FLEXIBLE MICROFLUIDIC TUBING. 用于制造长而灵活的微流体管道的 3d 微打印同轴喷嘴。

Proceedings. IEEE International Conference on Micro Electro Mechanical Systems Pub Date : 2024-01-01 Epub Date: 2024-02-22 DOI: 10.1109/mems58180.2024.10439296

Olivia M Young, Bailey M Felix, Mark D Fuge, Axel Krieger, Ryan D Sochol

{"title":"A 3D-MICROPRINTED COAXIAL NOZZLE FOR FABRICATING LONG, FLEXIBLE MICROFLUIDIC TUBING.","authors":"Olivia M Young, Bailey M Felix, Mark D Fuge, Axel Krieger, Ryan D Sochol","doi":"10.1109/mems58180.2024.10439296","DOIUrl":"10.1109/mems58180.2024.10439296","url":null,"abstract":"A variety of emerging applications, particularly those in medical and soft robotics fields, are predicated on the ability to fabricate long, flexible meso/microfluidic tubing with high customization. To address this need, here we present a hybrid additive manufacturing (or \"three-dimensional (3D) printing\") strategy that involves three key steps: (i) using the \"Vat Photopolymerization (VPP) technique, \"Liquid-Crystal Display (LCD)\" 3D printing to print a bulk microfluidic device with three inlets and three concentric outlets; (ii) using \"Two-Photon Direct Laser Writing (DLW)\" to 3D microprint a coaxial nozzle directly atop the concentric outlets of the bulk microdevice, and then (iii) extruding paraffin oil and a liquid-phase photocurable resin through the coaxial nozzle and into a polydimethylsiloxane (PDMS) channel for UV exposure, ultimately producing the desired tubing. In addition to fabricating the resulting tubing-composed of polymerized photomaterial-at arbitrary lengths (e.g., > 10 cm), the distinct input pressures can be adjusted to tune the inner diameter (ID) and outer diameter (OD) of the fabricated tubing. For example, experimental results revealed that increasing the driving pressure of the liquid-phase photomaterial from 50 kPa to 100 kPa led to fluidic tubing with IDs and ODs of 291±99 μm and 546±76 μm up to 741±31 μm and 888±39 μm, respectively. Furthermore, preliminary results for DLW-printing a microfluidic \"M\" structure directly atop the tubing suggest that the tubing could be used for \"ex situ DLW (esDLW)\" fabrication, which would further enhance the utility of the tubing.","PeriodicalId":91953,"journal":{"name":"Proceedings. IEEE International Conference on Micro Electro Mechanical Systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140121576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TOWARD CONTROLLED-RELEASE DRUG DELIVERY MICROCARRIERS ENABLED BY DIRECT LASER WRITING 3D PRINTING. 通过激光直接写入 3d 打印技术实现控释给药微载体。

Proceedings. IEEE International Conference on Micro Electro Mechanical Systems Pub Date : 2024-01-01 Epub Date: 2024-02-22 DOI: 10.1109/mems58180.2024.10439600

Sunandita Sarker, Kimia Forghani, Ziteng Wen, Ryan N Halli, Stephen Hoag, Sharon Flank, Ryan D Sochol

{"title":"TOWARD CONTROLLED-RELEASE DRUG DELIVERY MICROCARRIERS ENABLED BY DIRECT LASER WRITING 3D PRINTING.","authors":"Sunandita Sarker, Kimia Forghani, Ziteng Wen, Ryan N Halli, Stephen Hoag, Sharon Flank, Ryan D Sochol","doi":"10.1109/mems58180.2024.10439600","DOIUrl":"10.1109/mems58180.2024.10439600","url":null,"abstract":"Controlled-release, and especially long-acting, drug delivery systems hold promise for improving treatments for numerous medical conditions. Previously, we reported an additive manufacturing or \"three-dimensional (3D) printing\" approach for fabricating liquid-core-shell-cap microcarriers comprising standard photoresists. Here we explore the potential to extend this strategy to achieve microcarriers comprising biodegradable materials as a new pathway to controlled-release drug delivery options. Specifically, we investigate the use of \"Two-Photon Direct Laser Writing (DLW)\" as a means to 3D print microcarriers composed of: (i) a bottle-shaped \"shell\" with an orifice, (ii) an aqueous liquid \"core\", and (iii) a biodegradable \"cap\". The cap, which is DLW-printed directly onto the shell's orifice, is designed to degrade over time in the body-e.g., with degradation time proportional to cap thickness-to ultimately facilitate release of the liquid core at desired time points. Fabrication results based on the use of a biodegradable poly(ethylene glycol) diacrylate (PEGDA) photomaterial for the cap revealed that shell designs incorporating microfluidic obstruction structures appeared to limit undesired entry of the liquid-phase PEGDA into the shell (i.e., directly preceding cap printing), thereby resulting in improved retention of the liquid core after completion of the cap printing process. These results mark an important first step toward evaluating the utility of the presented DLW 3D printing strategy for possible drug delivery applications.","PeriodicalId":91953,"journal":{"name":"Proceedings. IEEE International Conference on Micro Electro Mechanical Systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140121577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FABRICATION OF MULTILUMEN MICROFLUIDIC TUBING FOR EX SITU DIRECT LASER WRITING. 制造用于原位直接激光写入的多腔微流体管。

Proceedings. IEEE International Conference on Micro Electro Mechanical Systems Pub Date : 2024-01-01 Epub Date: 2024-02-22 DOI: 10.1109/mems58180.2024.10439522

Bailey M Felix, Olivia M Young, Jordi T Andreou, Sunandita Sarker, Mark D Fuge, Axel Krieger, Clifford R Weiss, Christopher R Bailey, Ryan D Sochol

{"title":"FABRICATION OF MULTILUMEN MICROFLUIDIC TUBING FOR EX SITU DIRECT LASER WRITING.","authors":"Bailey M Felix, Olivia M Young, Jordi T Andreou, Sunandita Sarker, Mark D Fuge, Axel Krieger, Clifford R Weiss, Christopher R Bailey, Ryan D Sochol","doi":"10.1109/mems58180.2024.10439522","DOIUrl":"10.1109/mems58180.2024.10439522","url":null,"abstract":"Among the numerous additive manufacturing or \"three-dimensional (3D) printing\" techniques, two-photon Direct Laser Writing (DLW) is distinctively suited for applications that demand high geometric versatility with micron-to-submicron-scale feature resolutions. Recently, \"ex situ DLW (esDLW)\" has emerged as a powerful approach for printing 3D microfluidic structures directly atop meso/macroscale fluidic tubing that can be manipulated by hand; however, difficulties in creating custom esDLW-compatible multilumen tubing at such scales has hindered progress. To address this impediment, here we introduce a novel methodology for fabricating submillimeter multilumen tubing for esDLW 3D printing. Preliminary fabrication results demonstrate the utility of the presented strategy for resolving 743 μm-in-diameter tubing with three lumens-each with an inner diameter (ID) of 80 μm. Experimental results not only revealed independent flow of discrete fluorescently labelled fluids through each of the three lumens, but also effective esDLW-printing of a demonstrative 3D \"MEMS\" microstructure atop the tubing. These results suggest that the presented approach could offer a promising pathway to enable geometrically sophisticated microfluidic systems to be 3D printed with input and/or output ports fully sealed to multiple, distinct lumens of fluidic tubing for emerging applications in fields ranging from drug delivery and medical diagnostics to soft surgical robotics.","PeriodicalId":91953,"journal":{"name":"Proceedings. IEEE International Conference on Micro Electro Mechanical Systems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A BIOCOMPATIBLE GLASS-ENCAPSULATED TRIAXIAL FORCE SENSOR FOR IMPLANTABLE TACTILE SENSING APPLICATIONS. 用于植入式触觉传感应用的生物兼容玻璃封装三轴力传感器。

Proceedings. IEEE International Conference on Micro Electro Mechanical Systems Pub Date : 2024-01-01 Epub Date: 2024-02-22 DOI: 10.1109/mems58180.2024.10439390

Yixiao Ding, Lin Du, Han Hao, Thomas C E Mier, Jan Van der Spiegel, Timothy H Lucas, Firooz Aflatouni, Andrew G Richardson, Mark G Allen

引用次数: 0

Direct Interfacing of Neurons to Highly Integrated Microsystems 神经元与高度集成微系统的直接接口

Proceedings. IEEE International Conference on Micro Electro Mechanical Systems Pub Date : 2017-02-28 DOI: 10.1109/MEMSYS.2017.7863375

Andreas Hierlemann

引用次数: 3