Book of Proceedings: 1st International Conference on Chemo and BioInformatics,最新文献_第5页

MESOPOROUS SILICA AS A VEHICLE FOR ORGANOTIN(IV) COMPOUNDS 介孔二氧化硅作为有机锡(iv)化合物的载体

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.060k

G. Kaluđerović

引用次数: 0

POTENTIALLY TOXIC ELEMENTS IN LOWLAND GREAT MORAVA RIVER – BIOINDICATION WITH BLEAK (ALBURNUS ALBURNUS) 低地大摩拉瓦河中潜在的有毒元素。暗淡的生物指示

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.097m

Aleksandra M. Milošković, Nataša M. Kojadinović, Milena D. Radenković, Simona R. Đuretanović, T. Veličković, Marijana Nikolić, V. Simić

{"title":"POTENTIALLY TOXIC ELEMENTS IN LOWLAND GREAT MORAVA RIVER – BIOINDICATION WITH BLEAK (ALBURNUS ALBURNUS)","authors":"Aleksandra M. Milošković, Nataša M. Kojadinović, Milena D. Radenković, Simona R. Đuretanović, T. Veličković, Marijana Nikolić, V. Simić","doi":"10.46793/iccbi21.097m","DOIUrl":"https://doi.org/10.46793/iccbi21.097m","url":null,"abstract":"The utilization of fish bioindicators has become of crucial importance for ecosystem contamination assessment. Bleak (Alburnus alburnus) is a widely distributed epipelagic fish species characterized as very active with fast metabolism which can lead to a high accumulation of pollutants in tissues. Bleak is easy to sample, identify in the field by morphological characteristics, and easy to work within the laboratory. This study deals with the bleak sensitivity in detecting the pressure of the potentially toxic elements (PTEs) in the large lowland Great Morava River. The Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sn, and Zn were estimated in whole body composite (wbc) of bleak. The study was conducted at Great Morava River, near the city of Paraćin in Serbia. The highest concentrations of essential elements Zn and Fe were detected. The most toxic elements As and Hg were below detection limits. The metal pollution index (MPI) was 0.39. In large lowland rivers, point sources of pollution such as municipal wastewaters of smaller settlements usually have only local impact due to high dilution by the main watercourse. Since bleak is a site-specific species, results in this study indicated that this stretch of the Great Morava River is slightly affected by PTEs. None of the elements above the maximum permitted concentrations (MPC) in fish meat for use in the human diet, prescribed by the Official Gazette and European Commission Regulation, were not recorded.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88801334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETERMINATION OF HEAVY METALS AND SECONDARY METABOLITES OF „PEUCEDANUM OREOSELINUM“ (APIACEAE) 芍药中重金属及次生代谢物的测定

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.206p

M. Pavlović, Z. Simić, G. Đelić

{"title":"DETERMINATION OF HEAVY METALS AND SECONDARY METABOLITES OF „PEUCEDANUM OREOSELINUM“ (APIACEAE)","authors":"M. Pavlović, Z. Simić, G. Đelić","doi":"10.46793/iccbi21.206p","DOIUrl":"https://doi.org/10.46793/iccbi21.206p","url":null,"abstract":"The total quantity of metals (Mg, Ca, Ni, Fe, Mn, Zn, Cu) in soil samples and in sixteen different extracts from plant parts of Peucedanum oreoselinum (L.) Moench as well as the content of total phenols and flavonoids in plant extracts was determined. The contents of metals were determined by the atomic absorption spectrometer. Based on the average values of the metal concentration in the soil, they could be arranged in the following sequence: Fe > Ca > Mg > Mn > Zn > Cu > Ni. Soil concentrations of all tested metals were lower than the maximum allowed concentration. The results demonstrated that the analyzed plant extracts contained higher quantities of Ni and Ca. Although the studied species accumulate analyzed metals in different quantities, they are not hyperaccumulators of these metals. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 1.94 to P. oreoselinum, hyperaccumulation, phenols, flavonoids32.38 mg GA/g. The amounts of flavonoids in plant extracts were in range from 0.69 to 25.83 mg RU/g. We examined the correlation of metals and the phenolic compounds content in the extracts. According to our results the use P. oreoselinum for tea preparation is safe to a great extent for people, because in spite of the determined metal absorption by plant organs, the tea does not contain dangerous quantity of heavy metals. Also, it is suitable for the preparation of teas and herbal extracts due to minimal content of toxic metal (Ni), phenols and flavonoids.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84112873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRELIMINARY RESULTS REGARDING PHYLOGENY OF THE NOBLE CRAYFISH (DECAPODA, ASTACIDAE, „ASTACUS ASTACUS“) IN SERBIA 标题塞尔维亚珍稀小龙虾(十足目，虾蛄科，虾蛄科)系统发育的初步结果

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.222dj

Simona R. Đuretanović, T. Veličković, Aleksandra M. Milošković, Milena D. Radenković, Marijana Nikolić, I. Maguire, V. Simić

{"title":"PRELIMINARY RESULTS REGARDING PHYLOGENY OF THE NOBLE CRAYFISH (DECAPODA, ASTACIDAE, „ASTACUS ASTACUS“) IN SERBIA","authors":"Simona R. Đuretanović, T. Veličković, Aleksandra M. Milošković, Milena D. Radenković, Marijana Nikolić, I. Maguire, V. Simić","doi":"10.46793/iccbi21.222dj","DOIUrl":"https://doi.org/10.46793/iccbi21.222dj","url":null,"abstract":"The noble crayfish is one of the three autochthonous species that inhabit the freshwater ecosystems of Serbia, along with stone and Danube crayfish. The noble crayfish has a complex historical and genetic status shaped by geological events, habitat loss, pollution, translocations, and reintroductions of both autochthonous and allochthonous crayfish species. That led to the disruption of the species genetic structure, mixing, and loss of populations across Europe. According to recent data, its populations in the freshwater ecosystems of Serbia are significantly reduced, so it has the status of a \"strictly protected species\". The genetic structure of the species must be known for endangered species conservation. Unfortunately, there is lack of such data for the territory of Serbia, which due to its position on the Balkan Peninsula, was an important refuge during the glaciation period. In this paper, the genetic structure of seven crayfish populations in freshwater ecosystems of Serbia was examined. Analyzes were performed on the COI and 16S rRNA genes of mitochondrial DNA. The study results showed a significant diversity of COI and 16S rRNA haplotypes compared to already described haplotypes. Three haplotypes were detected, of which Hap26 is the most common and was detected in five studied populations. Haplotypes Hap47 and Hap49 were detected in one and two populations, respectively. The results obtained in this study, together with previously published morphometric data, represent a good starting point for further genetic and population research, which are the basis for the proposal of conservation measures.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91186718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA/BSA BINDING STUDY OF DINUCLEAR GOLD(III) COMPLEXES WITH AROMATIC NITROGEN-CONTAINING HETEROCYCLES AS BRIDGING LIGANDS 以芳香含氮杂环为桥接配体的双核金配合物的Dna / bsa结合研究

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.312a

Tina P. Andrejević, Darko P Ašanin, Nada D. Savić, N. Stevanović, M. Djuran, Biljana Đ. Glišić

{"title":"DNA/BSA BINDING STUDY OF DINUCLEAR GOLD(III) COMPLEXES WITH AROMATIC NITROGEN-CONTAINING HETEROCYCLES AS BRIDGING LIGANDS","authors":"Tina P. Andrejević, Darko P Ašanin, Nada D. Savić, N. Stevanović, M. Djuran, Biljana Đ. Glišić","doi":"10.46793/iccbi21.312a","DOIUrl":"https://doi.org/10.46793/iccbi21.312a","url":null,"abstract":"In recent decades, a special attention has been devoted to gold(III) complexes as potential antitumor agents due to their structural similarity to platinum(II) complexes. One of the possible mechanisms of the mode of antitumor activity of gold(III) complexes could include their interaction with DNA. However, the effectiveness of the therapeutic agents also depends on the degree of its binding to proteins present in the blood plasma, because, in this way, it is transported to the cell. Considering this, we investigated the interactions of three dinuclear gold(III) complexes of the general formula [{AuCl3}2(μ– L)], L = 4,4’-bipy (4,4’-bipyridine, Au1), bpe (1,2-bis(4-pyridyl)ethane, Au2) and dpe (1,2-bis(4- pyridyl)ethene, Au3) with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA). The main aim of the study was to evaluate the binding affinities of gold(III) complexes Au1–3 towards these biomolecules for possible insights on their mode of biological activity. The values of binding constants (KA) of Au1–3 to ct-DNA are higher than those for BSA, indicating greater affinity of the complexes towards this nucleic acid. The partition coefﬁcient (logP) value for Au1 is higher compared to the corresponding values for the other two complexes, what is in accordance with a higher cellular uptake efficiency of this complex.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90509944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFECTS OF SELENITE ON FILAMENTOUS FUNGI LIPID DROPLETS MONITORED „IN VIVO“ LABEL FREE USING ADVANCED NONLINEAR MICROSCOPY TECHNIQUE 2021ICCBIKG (2021) 亚硒酸盐对丝状真菌脂滴的影响，采用先进的非线性显微镜技术监测“体内”无标签

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.300p

Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić

{"title":"THE EFFECTS OF SELENITE ON FILAMENTOUS FUNGI LIPID DROPLETS MONITORED „IN VIVO“ LABEL FREE USING ADVANCED NONLINEAR MICROSCOPY TECHNIQUE 2021ICCBIKG (2021)","authors":"Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić","doi":"10.46793/iccbi21.300p","DOIUrl":"https://doi.org/10.46793/iccbi21.300p","url":null,"abstract":"Third Harmonic Generation (THG) microscopy was employed as a method of choice for lipid droplet (LD) measurements and quantification of the effect of selenite on LDs. Nonlinear laser scanning microscopy (NLSM) employs ultra-short laser pulses for imaging. THG microscopy is the modality of NLSM. Strong THG signals can only be observed from regions with non- uniformities with respect to their refractive index. Such regions in biological samples are lipid-water interfaces, and by far the brightest features in cells are LDs. For that reason, THG microscopy is the appropriate method for imaging of LDs from live unfixed cells, without the need for additional labeling. The biological effects of spore- to- end- of- exponential- phase duration (27 – 30 h) of exposure to 1 mM selenite were monitored in vivo on the cells of filamentous fungi in liquid culture. We measured the lipid droplet density and size distribution in a model fungi Phycomyces blakesleeanus. The in-house built microscope frame complemented with Yb KGW laser (1040 nm, 200 fs pulses) was used, while detection was enabled in the transmission arm by PMT through the Hoya glass UV filter (peak at 340 nm). From THG images of control and Se+4–treated hyphae, LD size and number were measured, showing that LD density was increased by more than 60% in Se+4–treated hyphae, compared to control. The average LD size distribution seemed slightly changed by Se+4 -treatment. The obtained results suggest that 1 mM selenite treatment probably induces cellular stress response in filamentous fungi.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84275200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETAILED GC-MS ANALYSES OF THE „MYRTUS COMMUNIS“ L., ESSENTIAL OIL AND THE ANTIFUNGAL ACTIVITY ASSESSMENT 桃金娘挥发油的gc-ms分析及抗真菌活性评价

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.276f

S. Filipović, T. Vasić, N. Radulović, D. Jevremović, I. Stanojević

{"title":"DETAILED GC-MS ANALYSES OF THE „MYRTUS COMMUNIS“ L., ESSENTIAL OIL AND THE ANTIFUNGAL ACTIVITY ASSESSMENT","authors":"S. Filipović, T. Vasić, N. Radulović, D. Jevremović, I. Stanojević","doi":"10.46793/iccbi21.276f","DOIUrl":"https://doi.org/10.46793/iccbi21.276f","url":null,"abstract":"Myrtus communis L., Myrtaceae, known as true myrtle, is a widely distributed evergreen shrub native to Mediterranean regions. This medicinal plant, used worldwide, possess a broad spectrum of phytochemical, pharmacological and therapeutic effects, mostly due to a large number of up to now detected, or isolated, essential oil components, considered the main biologically active ones. Multifarious secondary metabolite content, dependent on a geographic region, season of harvest and the length of distillation, depicts the variability of the essential oil composition obtained in general from its leaves, branches, fruits and flowers. Herein, we present the results of meticulous analyses of the essential oil (2.2%, w/w) constituents obtained from M. communis leaves, collected during the summer period from the coastal regions of peninsula Luštica, Montenegro. Detailed GC-MS analyses enabled the identification of 66 constituents, among which 1,8-cineole (28.4%), linalool (18.4%), α-pinene (16.6%), geranyl acetate (6.6%), α-terpineol (6.3%) and linalyl acetate (4.2%) were the major ones. Together with the secondary metabolite profile, determination of the Montenegro true myrtle essential oil agricultural plant protection potential is also estimated, by assessing mycelia growth of Colletotrichum acutatum J.H. Simmonds C.A.2 isolates, causative of strawberry antrachnose, in vitro.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"182 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81573336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE INFLUENCE OF SELENITE ON FILLAMENTOUS FUNGI HYPHA MORPHOMETRY PARAMETERS 亚硒酸盐对填充真菌菌丝形态测定参数的影响

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.308p

Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić

{"title":"THE INFLUENCE OF SELENITE ON FILLAMENTOUS FUNGI HYPHA MORPHOMETRY PARAMETERS","authors":"Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić","doi":"10.46793/iccbi21.308p","DOIUrl":"https://doi.org/10.46793/iccbi21.308p","url":null,"abstract":"Selenium salts have been known for long time to have a potential for both beneficial and harmful effects on living organisms. It is present in the environment, where it can be readily assimilated by plants and fungi, thus entering the food chain. We investigated the cell growth dynamics in the presence of selenite which is considered to have more toxic potential than selenate. The effects of selenite (1 mM) on the growth of fungi from the activated spores to the end of the exponential growth were measured on several hypha morphological parameters by microscopy in vivo. Phycomyces blaekesleneeanus was used as model filamentous fungus. The most striking effect of Se+4 treatment was inhibition of hypha growth, resulting in more than four times shorter hypha in Se+4 –treatment group than in the control (200 ± 50 µm, n = 50 vs 900 ± 100 µm, n = 40 respectively) at the end of exponential growth period under controlled conditions. The Se+4 effect was an inhibition and not a simple delay in growth, as hypha length did not change significantly from 27th to 30th hour of culture in Se+4-treatment group. Since the microscopy was performed on live cultured cells, undisturbed cytoplasmic streaming was observed, confirming that hyphae were alive at all time points measured. 30h old spore diameters were also significantly reduced by Se+4 treatment (p = 0.0365), while hypha diameters were not significantly altered.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86765680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QSRR MODELING OF LIQUID CHROMATOGRAPHY SEPARATION OF ZIPRASIDONE COMPOUNDS 齐拉西酮类化合物液相色谱分离的QSRR建模

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.284c

Marija Čarapić, K. Nikolić, D. Agbaba

引用次数: 0

PHENOLIC CONTENT AND POTENTIAL APPLICATION OF „LYSIMACHIA VULGARIS“ L. AERIAL PART AND ROOT EXTRACTS “淫羊藿”地上部和根提取物酚类物质含量及其潜在应用价值

Book of Proceedings: 1st International Conference on Chemo and BioInformatics, Pub Date : 2021-01-01 DOI: 10.46793/iccbi21.395s

N. Srećković, Jelena S. Katanić Stanković, V. Mihailović

{"title":"PHENOLIC CONTENT AND POTENTIAL APPLICATION OF „LYSIMACHIA VULGARIS“ L. AERIAL PART AND ROOT EXTRACTS","authors":"N. Srećković, Jelena S. Katanić Stanković, V. Mihailović","doi":"10.46793/iccbi21.395s","DOIUrl":"https://doi.org/10.46793/iccbi21.395s","url":null,"abstract":"Lysimachia vulgaris L., also known as yellow loosestrife, is an herbaceous perennial flowering plant from the family Primulaceae. Traditionally, L. vulgaris has been commonly used in Chinese medicine to increase urination, reduce fever, and treat sexually transmitted ailments. This study aimed to investigate phenolic content, antioxidant activity, and antimicrobial potential of L. vulgaris aerial part (LVA) and root (LVR) methanol extracts. Based on the spectrophotometric determination, both extracts possessed a high level of phenolic compounds. The content of total phenolic compounds (130.14 mg GAE/g) and condensed tannins (120.58 mg GAE/g) was higher in LVR, while total flavonoid (206.93 mg QUE/g) and flavanol (17.02 RUE/g) contents were higher in LVA. DPPH and ABTS assays were used to estimate the antioxidant activity of the extracts, whereby LVR showed better antioxidant potential in both applied methods. The extracts inhibited the growth of most of the tested bacterial and fungal strains with minimal inhibitory concentration values (MICs) ranged between (˂ 0.3125 to 20 mg/mL), whereby the significantly higher antimicrobial activity of LVR was observed. Based on the obtained results, the significant antioxidant and antimicrobial activity of L. vulgaris root extract may be a basis for its further research and potential application in the pharmaceutical and food industry.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86108045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0