BiosafetyPub Date : 2013-01-01Epub Date: 2013-01-17DOI: 10.4172/2167-0331.S1-002
K Allison Perry, Heather A O'Connell, Laura J Rose, Judith A Noble-Wang, Matthew J Arduino
{"title":"Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with <i>Bacillus anthracis</i> Spores.","authors":"K Allison Perry, Heather A O'Connell, Laura J Rose, Judith A Noble-Wang, Matthew J Arduino","doi":"10.4172/2167-0331.S1-002","DOIUrl":"https://doi.org/10.4172/2167-0331.S1-002","url":null,"abstract":"<p><p>The effect of packaging, shipping temperatures and storage times on recovery of <i>Bacillus anthracis</i>. Sterne spores from swabs was investigated. Macrofoam swabs were pre-moistened, inoculated with <i>Bacillus anthracis</i> spores, and packaged in primary containment or secondary containment before storage at -15°C, 5°C, 21°C, or 35°C for 0-7 days. Swabs were processed according to validated Centers for Disease Control/Laboratory Response Network culture protocols, and the percent recovery relative to a reference sample (T<sub>0</sub>) was determined for each variable. No differences were observed in recovery between swabs held at -15° and 5°C, (p ≥ 0.23). These two temperatures provided significantly better recovery than swabs held at 21°C or 35°C (all 7 days pooled, p ≤ 0.04). The percent recovery at 5°C was not significantly different if processed on days 1, 2 or 4, but was significantly lower on day 7 (day 2 vs. 7, 5°C, 10<sup>2</sup>, p=0.03). Secondary containment provided significantly better percent recovery than primary containment, regardless of storage time (5°C data, p ≤ 0.008). The integrity of environmental swab samples containing <i>Bacillus anthracis</i> spores shipped in secondary containment was maintained when stored at -15°C or 5°C and processed within 4 days to yield the optimum percent recovery of spores.</p>","PeriodicalId":91457,"journal":{"name":"Biosafety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34508084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}