Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with Bacillus anthracis Spores.

Biosafety Pub Date : 2013-01-01 Epub Date: 2013-01-17 DOI:10.4172/2167-0331.S1-002
K Allison Perry, Heather A O'Connell, Laura J Rose, Judith A Noble-Wang, Matthew J Arduino
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引用次数: 6

Abstract

The effect of packaging, shipping temperatures and storage times on recovery of Bacillus anthracis. Sterne spores from swabs was investigated. Macrofoam swabs were pre-moistened, inoculated with Bacillus anthracis spores, and packaged in primary containment or secondary containment before storage at -15°C, 5°C, 21°C, or 35°C for 0-7 days. Swabs were processed according to validated Centers for Disease Control/Laboratory Response Network culture protocols, and the percent recovery relative to a reference sample (T0) was determined for each variable. No differences were observed in recovery between swabs held at -15° and 5°C, (p ≥ 0.23). These two temperatures provided significantly better recovery than swabs held at 21°C or 35°C (all 7 days pooled, p ≤ 0.04). The percent recovery at 5°C was not significantly different if processed on days 1, 2 or 4, but was significantly lower on day 7 (day 2 vs. 7, 5°C, 102, p=0.03). Secondary containment provided significantly better percent recovery than primary containment, regardless of storage time (5°C data, p ≤ 0.008). The integrity of environmental swab samples containing Bacillus anthracis spores shipped in secondary containment was maintained when stored at -15°C or 5°C and processed within 4 days to yield the optimum percent recovery of spores.

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储存对炭疽芽孢杆菌环境表面采样标本完整性的影响。
包装、运输温度和贮存时间对炭疽芽孢杆菌恢复的影响。研究了拭子中的孢子。将Macrofoam拭子预湿润,接种炭疽芽孢杆菌孢子,包装在一级容器或二级容器中,然后在-15°C、5°C、21°C或35°C条件下保存0-7天。根据经过验证的疾病控制中心/实验室反应网络培养方案处理拭子,并确定每个变量相对于参考样本的回收率(T0)。在-15°C和5°C条件下,拭子的恢复无差异(p≥0.23)。这两种温度比21°C或35°C温度下的拭子回收率显著提高(共7天,p≤0.04)。如果在第1、2或4天处理,在5°C下的回收率没有显著差异,但在第7天显著降低(第2天vs. 7,5°C, 102, p=0.03)。无论储存时间如何(5°C数据,p≤0.008),二次收容提供的回收率明显高于一次收容。在-15°C或5°C条件下保存,并在4天内进行处理,以产生最佳的孢子回收率,可保持装运的含有炭疽芽孢杆菌孢子的环境棉签样本的完整性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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