Biophysics (Nagoya-shi, Japan)最新文献

Revealing the peptide presenting process of human leukocyte antigen through the analysis of fluctuation. 通过波动分析揭示人白细胞抗原的肽递呈过程。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-04-18 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.103

Saeko Yanaka, Takamasa Ueno, Kouhei Tsumoto, Kenji Sugase

引用次数: 2

How do chaperonins fold protein? 伴侣蛋白是如何折叠蛋白质的?

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-04-01 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.93

Fumihiro Motojima

引用次数: 19

Experimental and theoretical bases for mechanisms of antigen discrimination by T cells. T细胞抗原识别机制的实验和理论基础。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-03-26 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.85

Masashi K Kajita, Ryo Yokota, Kazuyuki Aihara, Tetsuya J Kobayashi

引用次数: 4

A protocol for preparing nucleotide-free KaiC monomer. 制备无核苷酸KaiC单体的方法。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-03-21 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.79

Atsushi Mukaiyama, Masato Osako, Takaaki Hikima, Takao Kondo, Shuji Akiyama

引用次数: 5

A new antigenic marker specifically labels a subpopulation of the class II Kenyon cells in the brain of the European honeybee Apis mellifera. 一种新的抗原标记物专门标记了欧洲蜜蜂大脑中II类凯尼恩细胞的一个亚群。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-03-19 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.73

Takayuki Watanabe, Takeo Kubo

{"title":"A new antigenic marker specifically labels a subpopulation of the class II Kenyon cells in the brain of the European honeybee Apis mellifera.","authors":"Takayuki Watanabe, Takeo Kubo","doi":"10.2142/biophysics.11.73","DOIUrl":"https://doi.org/10.2142/biophysics.11.73","url":null,"abstract":"The mushroom bodies are the higher-order integration center in the insect brain and are involved in higher brain functions such as learning and memory. In the social hymenopteran insects such as honeybees, the mushroom bodies are the prominent brain structures. The mushroom bodies are composed of lobed neuropils formed by thousands of parallel-projecting axons of intrinsic neurons, and the lobes are divided into parallel subdivisions. In the present paper, we report a new antigenic marker to label a single layer in the vertical lobes of the European honeybee Apis mellifera. In the brain of A. mellifera, a monoclonal antibody (mAb) 15C3, which was originally developed against an insect ecdysone receptor (EcR) protein, immunolabels a single layer of the vertical lobes that correspond to the most dorsal layer of the γ-lobe. The 15C3 mAb recognizes a single ~200 kDa protein expressed in the adult honeybee brain. In addition, the 15C3 mAb immunoreactivity was also observed in the lobes of the developing pupal mushroom bodies. Since γ-lobe is well known to their extensive reorganization that occurs during metamorphosis in Drosophila, the novel antigenic marker for the honeybee γ-lobe allows us to investigate morphological changes of the mushroom bodies during metamorphosis. ","PeriodicalId":91456,"journal":{"name":"Biophysics (Nagoya-shi, Japan)","volume":"11 ","pages":"73-7"},"PeriodicalIF":0.0,"publicationDate":"2015-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2142/biophysics.11.73","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34733422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro directed evolution of alpha-hemolysin by liposome display. 脂质体展示法在体外定向进化α -溶血素。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-03-04 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.67

Satoshi Fujii, Tomoaki Matsuura, Tetsuya Yomo

{"title":"In vitro directed evolution of alpha-hemolysin by liposome display.","authors":"Satoshi Fujii, Tomoaki Matsuura, Tetsuya Yomo","doi":"10.2142/biophysics.11.67","DOIUrl":"https://doi.org/10.2142/biophysics.11.67","url":null,"abstract":"We have developed a method to enable in vitro directed evolution that can be applied to membrane proteins. This method, termed liposome display, uses liposomes as compartments in which membrane proteins are synthesized and as scaffolds for membrane protein integration. Thus, the synthesized membrane proteins are displayed on the surface of the liposome and exhibit their functions. A randomly mutated DNA library of the membrane protein was generated, encapsulated in the liposomes at the single-molecule level, and used to generate a liposome library. Liposomes displaying the desired membrane protein function were selected, thus accumulating the DNA molecule encoding the desired membrane protein. We have applied this method to alpha-hemolysin, a membrane protein derived from Staphylococcus aureus. Alpha-hemolysin forms a nanopore in the membrane, which allows the penetration of small molecules. We aimed to improve this nanopore activity by using the liposome display method. Consequently, alpha-hemolysin evolved and attained a higher specific affinity for the liposome membrane. In this review, we describe the essential characteristics of liposome display and the properties of the evolved alpha-hemolysin obtained by this method. ","PeriodicalId":91456,"journal":{"name":"Biophysics (Nagoya-shi, Japan)","volume":"11 ","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2015-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2142/biophysics.11.67","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34733421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

FTIR study of primate color visual pigments. 灵长类动物彩色视觉色素的FTIR研究。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-03-04 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.61

Kota Katayama, Hideki Kandori

{"title":"FTIR study of primate color visual pigments.","authors":"Kota Katayama, Hideki Kandori","doi":"10.2142/biophysics.11.61","DOIUrl":"https://doi.org/10.2142/biophysics.11.61","url":null,"abstract":"How do we distinguish colors? Humans possess three color pigments; red-, green-, and blue-sensitive proteins, which have maximum absorbance (λmax) at 560, 530, and 420 nm, respectively, and contribute to normal human trichromatic vision (RGB). Each color pigments consists of a different opsin protein bound to a common chromophore molecule, 11-cis-retinal, whereas different chromophore-protein interactions allow preferential absorption of different colors. However, detailed experimental structural data to explain the molecular basis of spectral tuning of color pigments are lacking, mainly because of the difficulty in sample preparation. We thus started structural studies of primate color visual pigments using low-temperature Fourier-transform infrared (FTIR) spectroscopy, which needs only 0.3 mg protein for a single measurement. Here we report the first structural data of monkey red- (MR) and green- (MG) sensitive pigments, in which the information about the protein, retinal chromophore, and internal water molecules is contained. Molecular mechanism of color discrimination between red and green pigments will be discussed based on the structural data by FTIR spectroscopy. ","PeriodicalId":91456,"journal":{"name":"Biophysics (Nagoya-shi, Japan)","volume":"11 ","pages":"61-6"},"PeriodicalIF":0.0,"publicationDate":"2015-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2142/biophysics.11.61","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34344154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The activity of isolated snail neurons controlling locomotion is affected by glucose. 控制运动的离体蜗牛神经元的活动受葡萄糖的影响。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-03-03 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.55

Varvara Dyakonova, László Hernádi, Etsuro Ito, Taisia Dyakonova, Igor Zakharov, Dmitri Sakharov

{"title":"The activity of isolated snail neurons controlling locomotion is affected by glucose.","authors":"Varvara Dyakonova, László Hernádi, Etsuro Ito, Taisia Dyakonova, Igor Zakharov, Dmitri Sakharov","doi":"10.2142/biophysics.11.55","DOIUrl":"10.2142/biophysics.11.55","url":null,"abstract":"The involvement of serotonin in mediating hunger-related changes in behavioral state has been described in many invertebrates. However, the mechanisms by which hunger signals to serotonergic cells remain unknown. We tested the hypothesis that serotonergic neurons can directly sense the concentration of glucose, a metabolic indicator of nutritional state. In the snail Lymnaea stagnalis, we demonstrate that completely isolated pedal serotonergic neurons that control locomotion changed their biophysical characteristics in response to glucose application by lowering membrane potential and decreasing the firing rate. Additionally, the excitatory response of the isolated serotonergic neurons to the neuroactive microenvironment of the pedal ganglia was significantly lowered by glucose application. Because hunger has been reported to increase the activity of select neurons and their responses to the pedal ganglia microenvironment, these responses to glucose are in accordance with the hypothesis that direct glucose signaling is involved in the mediation of the hunger-related behavioral state. ","PeriodicalId":91456,"journal":{"name":"Biophysics (Nagoya-shi, Japan)","volume":"11 ","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2015-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34344153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lever arm extension of myosin VI is unnecessary for the adjacent binding state. 肌凝蛋白VI的杠杆臂伸展对于邻近结合状态是不必要的。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-02-28 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.47

Keigo Ikezaki, Tomotaka Komori, Yoshiyuki Arai, Toshio Yanagida

引用次数: 2

Preface of Special Issue "TRP channels: their functional roles in medical sciences". 特刊《TRP通道:在医学中的功能作用》前言。

Biophysics (Nagoya-shi, Japan) Pub Date : 2015-02-13 eCollection Date: 2015-01-01 DOI: 10.2142/biophysics.11.7

Etsuro Ito

引用次数: 0