BMC Complementary and Alternative Medicine最新文献

{"title":"Evaluating anthelmintic, anti-platelet, and anti-coagulant activities, and identifying the bioactive phytochemicals of Amaranthus blitum L.","authors":"Ghada Abdel-Moez, Hanaa Sayed, Azza Khalifa, Salwa Abd-Elrahman, Mohammed Osman, Shaymaa Mohamed","doi":"10.1186/s12906-024-04478-2","DOIUrl":"https://doi.org/10.1186/s12906-024-04478-2","url":null,"abstract":"Highlighting affordable alternative crops that are rich in bioactive phytoconstituents is essential for advancing nutrition and ensuring food security. Amaranthus blitum L. (AB) stands out as one such crop with a traditional history of being used to treat intestinal disorders, roundworm infections, and hemorrhage. This study aimed to evaluate the anthelmintic and hematologic activities across various extracts of AB and investigate the phytoconstituents responsible for these activities. In vitro anthelmintic activity against Trichinella spiralis was evaluated in terms of larval viability reduction. The anti-platelet activities were assessed based on the inhibitory effect against induced platelet aggregation. Further, effects on the extrinsic pathway, the intrinsic pathway, and the ultimate common stage of blood coagulation, were monitored through measuring blood coagulation parameters: prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT), respectively. The structures of isolated compounds were elucidated by spectroscopic analysis. Interestingly, a previously undescribed compound (19), N-(cis-p-coumaroyl)-ʟ-tryptophan, was isolated and identified along with 21 known compounds. Significant in vitro larvicidal activities were demonstrated by the investigated AB extracts at 1 mg/mL. Among tested compounds, compound 18 (rutin) displayed the highest larvicidal activity. Moreover, compounds 19 and 20 (N-(trans-p-coumaroyl)-ʟ-tryptophan) induced complete larval death within 48 h. The crude extract exhibited the minimal platelet aggregation of 43.42 ± 11.69%, compared with 76.22 ± 14.34% in the control plasma. Additionally, the crude extract and two compounds 19 and 20 significantly inhibited the extrinsic coagulation pathway. These findings extend awareness about the nutritional value of AB as a food, with thrombosis-preventing capabilities and introducing a promising source for new anthelmintic and anticoagulant agents.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Views of Hong Kong Chinese medicine practitioners on the application of the “Chinese Medicine Anti-epidemic Plans” prepared by the Chinese medicine expert group of central authorities: a focus group study","authors":"Shu Cheng Chen, Wing Fai Yeung, Hui Lin Cheng, Man Ho Li, Yuen Shan Ho","doi":"10.1186/s12906-024-04469-3","DOIUrl":"https://doi.org/10.1186/s12906-024-04469-3","url":null,"abstract":"Drawing on the extensive utilization of traditional Chinese medicine (TCM) to combat COVID-19 in Mainland China, experts designed a series of TCM anti-epidemic strategies. This study aims to understand Hong Kong CM practitioners’ application of and opinions on the “Chinese Medicine Anti-epidemic Plans.” Online focus group interviews were conducted, and purposive sampling was employed to invite 22 CM practitioners to voluntarily participate in three interview sessions. The interviews were audio recorded, then transcribed verbatim. The transcripts were analyzed using template analysis. Three themes were derived: (1) facilitators of the “Chinese Medicine Anti-epidemic Plans,” (2) barriers of the “Chinese Medicine Anti-epidemic Plans,” and (3) expectations on improving the “Chinese Medicine Anti-epidemic Plans.” The participants could obtain relevant information from various sources, which highlights the value of the plans for TCM medicinal cuisine and non-pharmacologic therapies and guiding junior CM practitioners, supplementing Western medicine interventions, and managing Chinese herb reserves in clinics. However, the barriers included the lack of a specialized platform for timely information release, defective plan content, limited reference value to experienced CM practitioners, and lack of applicability to Hong Kong. The expectations of the CM practitioners for improving the plans were identified based on the barriers. To enhance the implementation of the anti-epidemic plans, CM practitioners in Hong Kong expect to utilize a specific CM platform and refine the plans to ensure that they are realistic, focused, comprehensive, and tailored to the local context.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hand-press pellet on pain and daily life of elders with chronic lower back pain: randomized controlled trial","authors":"Hyojung Park, Hyejin Lee","doi":"10.1186/s12906-024-04481-7","DOIUrl":"https://doi.org/10.1186/s12906-024-04481-7","url":null,"abstract":"For elderly people with chronic lower back pain who need long-term management, there is a need for a nursing intervention study that is effective, is easy to perform, and applies complementary and alternative therapies to manage pain without repulsion. Hand pressure therapy is a treatment indigenous to Korea used to reduce pain and improve functions of daily life by applying acupuncture, pressure sticks, and moxibustion to parts of the hand as they relate to parts of the body. This research is to identify the effects of pellet pressed on the hand on pain and the daily lives of elders with chronic lower back pain (CLBP). The hand pressed-pellet intervention period was six weeks long. Twenty-seven patients in the intervention group and twenty-four patients in the placebo control group were recruited from elderly over sixty-five who used welfare centers. In the intervention group, hand pressed-pellet therapy was conducted in eleven acupressure response zones related to CLBP, and the placebo control group was provided with similar therapy and zones, but unrelated to CLBP. The research tool measured the intensity of CLBP using the Visual Analogue Scale (VAS), the Korean Owestry Disability Index (K-ODI), which are subjective indicators, and the Compact Digital Algometer, which is an objective indicator. The pain intensity (VAS) measured after six weeks of hand pressed-pellet therapy showed significant difference between the two groups compared to their pain before the experiment (F = 60.522, p < .001). There was a significant difference between the two groups in the pain pressure threshold using pressure statistics (F = 8.940, p < .001), and in CLBP dysfunction evaluation index (K-ODI) after applying pressed pellet to the hand (Z = − 3.540, p < .001). Subjective indicators were measured to verify the effect of hand pressed-pellet therapy on CLBP, and the result confirmed that the hand pressed-pellet therapy was effective in alleviating CLBP. The study was registered retrospectively with reference number KCT0008024 on 23/12/2022.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRECIS-2 analysis of pragmatic acupuncture trials: a systematic review","authors":"Jinwoong Lim, Hyeonhoon Lee, Yong-Suk Kim","doi":"10.1186/s12906-024-04473-7","DOIUrl":"https://doi.org/10.1186/s12906-024-04473-7","url":null,"abstract":"Pragmatic acupuncture trials (PATs) are a research tool for assessing the effectiveness of acupuncture treatments in a real-world setting. This study aimed to provide a comprehensive methodological analysis of PATs using the PRECIS-2(PRagmatic Explanatory Continuum Indicator Summary-2) tool to determine their pragmatism. The MEDLINE, EMBASE, Cochrane Central Register for Controlled Trials, CINAHL, Allied and Complementary Medicine Database, China National Knowledge Infrastructure, VIP, WANFANG, Taiwan Periodical Literature Database, KoreaMed, KMbase, Research Information Service System, Oriental Medicine Advanced Searching Integrated System, CiNii and ClinicalTrials.gov were searched. The search included randomised controlled trials (RCTs) and protocols of RCTs that investigated all types of acupuncture and used self-declared pragmatic design. Two authors independently collected the basic information and characteristics of the studies and assessed their pragmatism using the nine PRECIS-2 domains and the additional domain of control. A total of 93 studies were included. The means of eligibility, recruitment, organisation, primary outcome, primary analysis, and control domains were statistically larger than three and were shown to be pragmatic. The means of setting, flexibility:delivery, and follow-up domains were not greater than three and were shown to be non-pragmatic. For flexibility:adherence domain was inappropriate for assessment owing to insufficient information in the studies. PATs were pragmatic in the domain of eligibility, recruitment, organisation, primary outcome, primary analysis, and control and were not pragmatic in the domain of setting, flexibility:delivery, and follow-up. Future PATs need to strengthen the pragmatism in the setting, flexibility:delivery, and follow-up domains and to describe the flexibility:adherence domain in more detail. CRD42021236975.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of Dioscorea bulbifera Linn. extract and its active component flavanthrinin against skin-associated bacteria","authors":"Donruedee Sanguansermsri, Phanchana Sanguansermsri, Kittisak Buaban, Vachira Choommongkol, Chareeporn Akekawatchai, Noree Charoensri, Ian Fraser, Nalin Wongkattiya","doi":"10.1186/s12906-024-04480-8","DOIUrl":"https://doi.org/10.1186/s12906-024-04480-8","url":null,"abstract":"Dioscorea bulbifera Linn. has been used for wound care in Thailand. However, a comprehensive evaluation of its antibacterial activity is required. This study aimed to investigate the antibacterial efficacy of D. bulbifera extract against skin-associated bacteria and isolate and characterize its active antibacterial agent, flavanthrinin. Air-dried bulbils of D. bulbifera were pulverised and extracted with hexane, dichloromethane, ethyl acetate, methanol, ethanol, and distilled water; vacuum filtered; concentrated; freeze-dried; and stored at -20 ºC. Antibacterial activity of the extracts was assessed using microdilution techniques against several skin-associated bacteria. Thin-layer chromatography (TLC) bioautography was used to identify the active compounds in the extract, which were fractionated by column chromatography and purified by preparative TLC. The chemical structures of the purified compounds were analysed using nuclear magnetic resonance (NMR). The cytotoxicity of the extract and its active compounds was evaluated in Vero cells. The ethyl acetate extract exhibited distinct inhibition zones against bacteria compared to other extracts. Therefore, the ethyl acetate extract of D. bulbifera in the ethyl acetate layer was used for subsequent analyses. D. bulbifera extract exhibited antibacterial activity, with minimum inhibitory concentrations (MICs) of 0.78–1.56 mg/mL. An active compound, identified through TLC-bioautography, demonstrated enhanced antibacterial activity, with MICs of 0.02–0.78 mg/mL. NMR analysis identified this bioactive compound as flavanthrinin. Both D. bulbifera extract and flavanthrinin-containing fraction demonstrated potent antibacterial activity against Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), and S. epidermidis. The flavanthrinin containing fraction demonstrated low cytotoxicity against Vero cells, showing CC50 values of 0.41 ± 0.03 mg/mL. These values are lower than the MIC value, indicating that this fraction is safer than the initial ethyl acetate extract. Dioscorea bulbifera extract and its bioactive component flavanthrinin demonstrated significant antibacterial activity against the skin-associated bacteria Staphylococci, including MRSA. Flavanthrinin has potential as a complementary therapeutic agent for managing skin infections owing to its potent antibacterial effects and low cytotoxicity.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to “Physical activity and psychological support can replace “another pill” to manage cancer-related symptoms in children and adolescents diagnosed with cancer”","authors":"Dana C. Mora, Miek C. Jong, Sara A. Quandt, Thomas A. Arcury, Agnete E. Kristoffersen, Trine Stub","doi":"10.1186/s12906-024-04476-4","DOIUrl":"https://doi.org/10.1186/s12906-024-04476-4","url":null,"abstract":"<p>Thank you for the opportunity to respond to the letter to the editor submitted by Dr. Caru and his colleagues [1]. We appreciate the interest of Dr. Caru et al. in our paper and their knowledgeable comments on our study.</p><p>In their letter to the editor [1], Dr. Caru notes that the findings of our study [2] need to be put into perspective to increase its scope and highlight that physical activity and psychosocial interventions are important nonpharmacological interventions to manage cancer-related symptoms. Furthermore, they point out that our paper [2] did not discuss physical activity and psychological support as CAM modalities. We agree that the latter were not discussed in the paper to a great extent. However, this reflects the use of a qualitative interview design in which we focused on modalities that the participants themselves discussed in response to the queries and probes used to illicit their views on supportive care for children undergoing cancer treatment. The interviews were open-ended, and, while participants’ responses were probed for additional modalities, such an approach does not query about specific modalities not discussed by the participants. Some participants briefly mentioned physical activity, but it was not spoken about in enough detail or by enough participants to emerge as a salient theme in the data analysis. We briefly discuss psychosocial support in the results section under the play, psychodrama, and music therapy Sect. [2].</p><p>As stated in the paper’s methods section, the participants were recruited using purposive sampling [3] through professional pediatrics and CAM networks. One of the limitations of the paper was that it was not possible to interview providers for every CAM modality currently used. We agree that our study had limitations and think it is important to highlight modalities (such as physical activity and psychosocial interventions) that are safe and effective and can aid parents and children in coping with the adverse effects derived from cancer treatment.</p><p>In their letter, Dr. Caru and colleagues appear to criticize our paper because it did not include experts on physical activity. This criticism misses the point that we interviewed care providers with expetice in treating pediatric oncology patients not necessarily researchers. We do understand that there have been studies published of the effectiveness of physical activity interventions with child cancer patients [4]. However, this does not necessarily make such interventions top of mind with care providers, the population we interviewed. It is indeed significant that such interventions were NOT noted by our study participants. We suggest that this reflects the need for greater training of care providers in the effectiveness of such modalities, rather than a shortcoming of our study.</p><p>In conclusion, we agree with Dr. Caru and his colleagues that physical activity and psychosocial interventions are important nonpharmacological ","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140637472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity","authors":"Heyam Mohamed Ali Sidahmed, Ainnul Hamidah Syahadah Azizan, Syam Mohan, Mahmood Ameen Abdulla, Siddig Ibrahim Abdelwahab, Manal Mohamed Elhassan Taha, A. Hamid A. Hadi, Kamal Aziz Ketuly, Najihah Mohd Hashim, Mun Fai Loke, Jamuna Vadivelu","doi":"10.1186/s12906-024-04475-5","DOIUrl":"https://doi.org/10.1186/s12906-024-04475-5","url":null,"abstract":"<p><b>Retraction Note: BMC Complement Med Ther 13, 183 (2013)</b></p><p><b>https://doi.org/10.1186/1472-6882-13-183</b></p><p>The Editors retracted this article because of concerns regarding a number of figures presented in this work. These concerns call into question the article’s overall scientific soundness and its authorship. An investigation conducted after its publication discovered similarities between images in this work and images in [1] and [2],</p><ul>\u0000<li>\u0000<p>Panel D in Fig. 5 and panel B in Fig. 5 in [1];</p>\u0000</li>\u0000<li>\u0000<p>Panel D in Fig. 6 and panel E in Fig. 5 in [2];</p>\u0000</li>\u0000<li>\u0000<p>Panel F in Fig. 5 and panel E in Fig. 4 in [1];</p>\u0000</li>\u0000<li>\u0000<p>The bottom part of panel F in Fig. 5 and the top part of panel E in Fig. 4 in [2];</p>\u0000</li>\u0000</ul><p>The Editors therefore no longer have confidence in the integrity of the research presented in this article. Jamuna Vadivelu and Mun Fai Loke agree with the retraction. The remaining authors have not responded to correspondence from the Publisher about the retraction.</p><ol data-track-component=\"outbound reference\"><li data-counter=\"1.\"><p>Al Batran R, Al-Bayaty F, Ameen Abdulla M, Al-Obaidi J, Hajrezaei MM, Hassandarvish M, Fouad P, Golbabapour M, S. and, Talaee S. Gastroprotective effects of <i>Corchorus olitorius</i>. J Gastroenterol Hepatol. 2013;28:1321–9. https://doi.org/10.1111/jgh.12229.</p><p>Article PubMed PubMed Central Google Scholar </p></li><li data-counter=\"2.\"><p>Ismail IF, Golbabapour S, Hassandarvish P, Hajrezaie M, Majid NA, Kadir FA. Fouad Al-Bayaty, Khalijah Awang, Hazrina Hazni, Mahmood Ameen Abdulla, Gastroprotective Activity of <i>Polygonum chinense</i> Aqueous Leaf Extract on Ethanol-Induced Hemorrhagic Mucosal Lesions in Rats, <i>Evidence-Based Complementary and Alternative Medicine</i>, vol. 2012, Article ID 404012, 9 pages, 2012. https://doi.org/10.1155/2012/404012.</p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><span>Author notes</span><ol><li><p>Equal contributors.</p></li></ol><h3>Authors and Affiliations</h3><ol><li><p>Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia</p><p>Heyam Mohamed Ali Sidahmed, Syam Mohan &amp; Najihah Mohd Hashim</p></li><li><p>Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia</p><p>Mahmood Ameen Abdulla</p></li><li><p>Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia</p><p>Ainnul Hamidah Syahadah Azizan, A. Hamid A. Hadi &amp; Kamal Aziz Ketuly</p></li><li><p>Medical Research Centre, Jazan University, P.O. Box 114, Jazan, Saudi Arabia</p><p>Siddig Ibrahim Abdelwahab &amp; Manal Mohamed Elhassan Taha</p></li><li><p>Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Mal","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effectiveness of Propolis with chlorhexidine mouthwash on gingivitis – a randomized controlled clinical study","authors":"Shilpa Gunjal, Deepak Gowda Sadashivappa Pateel","doi":"10.1186/s12906-024-04456-8","DOIUrl":"https://doi.org/10.1186/s12906-024-04456-8","url":null,"abstract":"To assess and compare the effectiveness of propolis mouthwash with chlorhexidine mouthwash in the reduction of plaque and gingivitis. A single centre, latin-square cross-over, double masked, randomized controlled clinical trial was conducted on 45 chronic generalized gingivitis subjects who were chosen from the dental clinic of MAHSA University, Malaysia. A total of 45 subjects were randomly assigned into one of the three different groups (n = 15 each) using a computer-generated random allocation sequence: Group A Propolis mouthwash; Group B Chlorhexidine mouthwash; and Group C Placebo mouthwash. Supragingival plaque and gingival inflammation were assessed by full mouth Plaque index (PI) and gingival index (GI) at baseline and after 21 days. The study was divided into three phases, each phase lasted for 21 days separated by a washout period of 15 days in between them. Groups A, B and C were treated with 0.2% Propolis, Chlorhexidine, and Placebo mouthwash, respectively, in phase I. The study subjects were instructed to use the assigned mouthwash twice daily for 1 min for 21 days. On day 22nd, the subjects were recalled for measurement of PI and GI. After phase I, mouthwash was crossed over as dictated by the Latin square design in phase II and III. At baseline, intergroup comparison revealed no statistically significant difference between Groups A, B and C (p > 0.05). On day 21, one-way ANOVA revealed statistically significant difference between the three groups for PI (p < 0.001) and GI (p < 0.001). Bonferroni post-hoc test showed statistically significant difference between Propolis and Chlorhexidine mouthwash (P < 0.001), with higher reduction in the mean plaque and gingival scores in propolis group compared to chlorhexidine and placebo groups. Propolis mouthwash demonstrated significant improvement in gingival health and plaque reduction. Thus, it could be used as an effective herbal mouthwash alternative to chlorhexidine mouthwash. The trial was retrospectively registered on 25/07/2019 at clinicaltrials.gov and its identifier is NCT04032548.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Lippia scaberrima Sond. and Aspalathus linearis (Burm.f.) R. Dahlgren extracts on human CYP enzymes and gold nanoparticle synthesis: implications for drug metabolism and cytotoxicity","authors":"Anna-Mari Kok, Risto Juvonen, Markku Pasanen, Vusani Mandiwana, Michel Lonji Kalombo, Suprakas Sinha Ray, Rirhandzu Rikhotso-Mbungela, Namrita Lall","doi":"10.1186/s12906-024-04439-9","DOIUrl":"https://doi.org/10.1186/s12906-024-04439-9","url":null,"abstract":"Metabolism is an important component of the kinetic characteristics of herbal constituents, and it often determines the internal dose and concentration of these effective constituents at the target site. The metabolic profile of plant extracts and pure compounds need to be determined for any possible herb-drug metabolic interactions that might occur. Various concentrations of the essential oil of Lippia scaberrima, the ethanolic extract of Lippia scaberrima alone and their combinations with fermented and unfermented Aspalathus linearis extract were used to determine the inhibitory potential on placental, microsomal and recombinant human hepatic Cytochrome P450 enzymes. Furthermore, the study investigated the synthesis and characterization of gold nanoparticles from the ethanolic extract of Lippia scaberrima as a lead sample. Confirmation and characterization of the synthesized gold nanoparticles were conducted through various methods. Additionally, the cytotoxic properties of the ethanolic extract of Lippia scaberrima were compared with the gold nanoparticles synthesized from Lippia scaberrima using gum arabic as a capping agent. All the samples showed varying levels of CYP inhibition. The most potent inhibition took place for CYP2C19 and CYP1B1 with 50% inhibitory concentration (IC50) values of less than 0.05 µg/L for the essential oil tested and IC50-values between 0.05 µg/L-1 µg/L for all the other combinations and extracts tested, respectively. For both CYP1A2 and CYP2D6 the IC50-values for the essential oil, the extracts and combinations were found in the range of 1 – 10 µg/L. The majority of the IC50 values found were higher than 10 µg/L and, therefore, were found to have no inhibition against the CYP enzymes tested. Therefore, the essential oil of Lippia scaberrima, the ethanolic extract of Lippia scaberrima alone and their combinations with Aspalathus linearis do not possess any clinically significant CYP interaction potential and may be further investigated for their adjuvant potential for use in the tuberculosis treatment regimen. Furthermore, it was shown that the cytotoxic potential of the Lippia scaberrima gold nanoparticles was reduced by twofold when compared to the ethanolic extract of Lippia scaberrima.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigallocatechin-3-Gallate attenuates lipopolysacharide-induced pneumonia via modification of inflammation, oxidative stress, apoptosis, and autophagy","authors":"Meili Shen, Yuting You, Chengna Xu, Zhixu Chen","doi":"10.1186/s12906-024-04436-y","DOIUrl":"https://doi.org/10.1186/s12906-024-04436-y","url":null,"abstract":"Pneumonia, the acute inflammation of lung tissue, is multi-factorial in etiology. Hence, continuous studies are conducted to determine the mechanisms involved in the progression of the disease and subsequently suggest effective treatment. The present study attempted to evaluate the effects of Epigallocatechin-3-Gallate (EGCG), an herbal antioxidant, on inflammation, oxidative stress, apoptosis, and autophagy in a rat pneumonia model. Forty male Wistar rats, 5 months old and 250–290 g were divided into four groups including control, EGCG, experimental pneumonia (i/p LPS injection, 1 mg/kg), and experimental pneumonia treated with EGCG (i/p, 15 mg/kg, 1 h before and 3 h after LPS instillation). Total cell number in the bronchoalveolar lavage fluid, inflammation (TNF-a, Il-6, IL-1β, and NO), oxidative stress (Nrf2, HO-1, SOD, CAT, GSH, GPX, MDA, and TAC), apoptosis (BCL-2, BAX, CASP-3 and CASP-9), and autophagy (mTOR, LC3, BECN1) were evaluated. The findings demonstrated that EGCG suppresses the LPS-induced activation of inflammatory pathways by a significant reduction of inflammatory markers (p-value < 0.001). In addition, the upregulation of BCL-2 and downregulation of BAX and caspases revealed that EGCG suppressed LPS-induced apoptosis. Furthermore, ECGC suppressed oxidative injury while promoting autophagy in rats with pneumonia (p-value < 0.05). The current study revealed that EGCG could suppress inflammation, oxidative stress, apoptosis, and promote autophagy in experimental pneumonia models of rats suggesting promising therapeutical properties of this compound to be used in pneumonia management.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}