Journal of microbiology & experimentation最新文献

{"title":"Viability test exclusively is not adequate to evaluate the T4 Acanthamoeba keratitis’ treatment","authors":"Daniella de Sousa Mendes Moreira Alves, G. Bernardes, Daianny Costa da Silva, Luciano Moreira Alves, A. M. Castro, M. C. Vinaud","doi":"10.15406/JMEN.2020.08.00278","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00278","url":null,"abstract":"Acanthamoeba keratitis is a serious corneal infection and in some cases can lead to blindness.1 The T4 genotype is often associated with human infections by Acanthamoeba.2,3 The incidence of keratitis has increased considerably worldwide, especially those associated to contact lenses use.4,5 Cysts and recurrence of infection represent a challenge in the treatment of keratitis due to its high resistance to adverse conditions and to most medications.6,7 This intriguing property can lead to late diagnosis and can explain absence of cyst removal during and after therapy regimen.8 In early stage, due to symptomatology and incorrect diagnosis the disease can be inappropriately mistaken for keratitis by other microorganisms, such as fungi and bacteria, leading to treatment with antifungals or antibiotics that will not be effective. Therefore, delaying proper treatment for this infection can increase severity and risk of blindness.9","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":"8 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46905711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spondylodiscitis due to Brucella spp. case report ","authors":"Alvarez Delgado Karla Selene, Martinez Navarro Jovana","doi":"10.15406/jmen.2019.07.00276","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00276","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45618554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility of the deviants","authors":"Grishma V. Kulkarni","doi":"10.15406/jmen.2019.07.00275","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00275","url":null,"abstract":"Genetic variability is essential to occur evolution. The fitness of the organism depends on its capacity to adapt to the changing environmental conditions. Antimicrobial agents exert strong selective pressure on the bacterial population favoring the microorganisms that are capable of resisting them. Genetic variability may occur by variety of the mechanisms such as micro evolutionary changes in the form of point mutations and macro evolutionary changes in the large segment of the DNA which ultimately leads to acquired resistance and better survival of the organisms.1 Intrinsic resistance is also not an exception to this transformation as documented by HHS studies about Burkholderia pseudomallei and other Minimum inhibitory concentration (MIC) studies as well. Though B.pseuodomallei is known for its intrinsic resistance to aminoglycosides and macrolide, found to be susceptible to them due absence of or nonfunctional RND (resistance nodulation division) pumps.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42406104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid method for determining the antimicrobial susceptibility on chromogenic agar for urine specimen","authors":"Renata Lazo de la Vega Giraud, Estefania Guadalupe Alvarado Bravo, Gabriel Chavez Giraud, C. Rodríguez","doi":"10.15406/jmen.2019.07.00272","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00272","url":null,"abstract":"The traditional basis for the evaluation of urinary tract pathogens is the urine culture and the antibiotic susceptibility testing. The major drawback of the current microbiology approach is the time lapse of 2-3 days between specimen collection and the availability of the result with an objective evidence for treatment selection. Antibiotic resistance is a significant problem not only among nosocomial complicated UTIs, where it has traditionally been recognized, but also in community acquired simple UTIs. Early microbiological diagnosis and the correct administration of the appropriate initial antimicrobial therapy have proven to be associated with decrease rates of morbidity. Direct susceptibility testing of blood culture is being used in some microbiology laboratories, methods for urine cultures have been evaluated, some do not recommend the procedure, and others advocate a variety of conditions for acceptance of results. We performed a comparison between rapid method and standardized disk diffusion method in urine specimen, determining the antimicrobial susceptibility on CHROMagar Orientation Medium, without having to wait for bacterial isolation, our method may provide preliminary information that improves antimicrobial use and it also has a very good correlation with standard procedures. Methods","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47334094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological and physicochemical analyses of well water used for drinking in Ekpoma-Edo State, Nigeria","authors":"Osatohanmwen Osarenmwinda, Aruya Graceann Idaehor","doi":"10.15406/jmen.2019.07.00271","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00271","url":null,"abstract":"Portable water is a key requirement for human, whether it is intended for drinking, recreational activities and other domestic purposes. It is a vital desire for all life forms. It is therefore imperative that adequate amount of portable, clean and safe water be made available to other life forms such as flora and fauna. Inadequate quantity of it results to mobility and fatality rate in rural settlements where chemical contaminants and water-based infections are endemic and persistent because of poor groundwater and surface waters quality.1 The global health importance of water quality is a concept that needs not to be neglected as quite a number of infectious diseases are contracted by water via faecal-oral mode of transmission. These infections have been reported of having a fatality rate of 5 million children annually, causing 1/6th of the world population ill.2 Water borne infections emanate from intake of untreated contaminated water by pathogenic microbes. These infections are linked with the non-availability and accessibility to clean, portable water supply in addition to unhygienic vicinity. This affects man and the biotic components of the ecosystem especially in developing countries. The following bacteria genera are often incriminated in water based infections; Pseudomonas aeruginosa, Shigella sonnei, Salmonella, Klebsiella, lyanobacteria, Proteus, Vibrio, Mycobacteria, Streptococcus faecalis e.t.c.3 Fresh water which lies below the earth crust in broken segments of rocks and soil pore spaces is considered as groundwater. It is often regarded as an ideal source of water because it is seem not to be opaque and clean. This is attributed to its passage via various layers and sediments of rocks, which act as a sort of natural filtration system. However it portability and quality can be compromise as a result of poor source protection and resource management.4 There is an increase in the spite of ground water contamination especially in urban settlements with variety of industrial activities, increase in the number of inhabitants, poor hygiene, use of land for mechanized and commercial farming and indiscriminate disposal of wastes on land.5 The presence of contaminants whether inorganic or organic in the ground water above maximum limits sets by water regulatory agencies such as WHO, EPA, NIS and FEPA may cause a serious health calamity.6 Inhabitants of developing countries unavoidably still rely on contaminated ground water due to non availability of potable water sources.7 Water apart from its domestic applications has various other aspect of use such as transportation, generation of hydro-power electricity, irrigation and aquaculture. It is a major driving force that controls the evolution and functionality of the universe on earth.8 Varieties of artificial chemicals pollutants such as insecticides, pesticides, nitrates from fertilizers, sulphates, chlorides, phenols, soap and heavy metals e.t.c are chief contributors to water contaminat","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46628515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maastrichtian-paleogene benthic foraminifera from the Middle East and its distribution in the Tethys, a review","authors":"H. Anan","doi":"10.15406/jmen.2019.07.00270","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00270","url":null,"abstract":"The present paper aims to highlight the paleontology, stratigraphy paleogeography, paleoecology and biogeography of the 119 Maastrichtian-early Paleogene calcareous benthic foraminiferal species and subspecies which were originally erected by many authors from six Middle East countries (Maps 1 & 2): (1) Egypt: Nakkady,1-3 LeRoy,4 Said & Kenawy,5 Abdou & Abdel Kireem,6 Anan,7‒27 Aly et al.,28 (2) Jordan: Futyan,29 (3) Saudi Arabia: Hasson30 (4) Qatar: Hewaidy & Al-Hitmi31 (5) UAE: Anan32‒37 and (6) Pakistan: Haque,38‒40 Nomura & Brohi,41 and many studies are recorded some of these taxa in a wider geographic areas in the Tethys, from Atlantic to Indian Oceans via Mediterranean (Map 3).","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42947270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Kytococcus schroeteri prosthetic valve endocarditis in Oman","authors":"Wafa Nasser Al Tamtami, F. Al Yaquobi, A. Al Jardani, M. Al Riyami, M. Cormican","doi":"10.15406/jmen.2019.07.00268","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00268","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43423890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing filamentous fungi from Puerto Rico for the bioremediation of organic compounds","authors":"R. Giles","doi":"10.15406/jmen.2019.07.00266","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00266","url":null,"abstract":"We developed more difficult examination criteria (by the fungi’s perspective) by evaluating the fungal isolates’ ability to grow in minimal media with model toxins as their sole carbon source. We grew isolates on Petri plates containing minimal media plus either 0.02% v/v toluene or 0.02% v/v chlorobenzene. Surprisingly, we observed that several of our isolates exhibited healthy colony growth suggesting they were degrading the compounds as a sole carbon and micronutrient source.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48766413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sero-epidemiology of Plasmodium vivax infection among Sudanese's and Ethiopians in Khartoum State","authors":"M. Hamad, Mohanad E Alfadol, M. M. Eltayeb","doi":"10.15406/jmen.2019.07.00265","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00265","url":null,"abstract":"Plasmodium vivax is a hemo-pathogenic parasite that belongs for the phylum Apicomplexa. It causes malaria which is the public health significant. Most of the research and published literature on malaria focuses on Plasmodium falciparum and much less on Plasmodium vivax.1 This focus is due to the very high burden of mortality attributed to the falciparum species in Africa.2 However, there is growing evidence that Plasmodium vivax is responsible for a significant burden of disease worldwide accounting for half of all malaria cases in Asia and Latin America, will nearly 2.5 billion people at risk of infection.3 All species are transmitted by the bite of an infective female Anopheles mosquitoes control and management of vivax malaria is difficult, and the present particular challenges.4 Hematological changes that have been reported to accompany malaria are anemia, thrombocytopenia, leucopenia, atypical lymphocytosis, and infrequently disseminated intravascular coagulation.5 Some studies have shown that Plasmodium vivax can also cause severe disease and the hematological parameters are usually changed in such cases. The present study was undertaken to assess the hematological alterations in vivax malaria patients in comparison between Sudanese and Ethiopians.6","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43659480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual cause of hyponatremia in a young patient with varicella zoster infection: a case report","authors":"Juhi Chandwani, S. Kantor, Seif S Al Abri, Antara Gokhale, Sadanandan Prakash","doi":"10.15406/jmen.2019.07.00263","DOIUrl":"https://doi.org/10.15406/jmen.2019.07.00263","url":null,"abstract":"Hyponatremia, defined as serum sodium below 135mEq/L, is one of the most frequently encountered electrolyte disorders in critically ill patients.1 Patients at risk include septic patients, postoperative patients, older patients on thiazide diuretics, patients with malignant or psychiatric illness, and endurance athletes. A serum sodium concentration <100mEq/l, carries a high risk of brain damage due to seizure activity. There is concern about injury to the brain for rapid and over correction of hyponatremia. We present here a patient who presented with Varicella Zoster infection and symptomatic hyponatremia due to volitional intake of free water as per advice of homeopathy physician. Hypertonic saline, fluid restriction and close monitoring in the intensive care unit led to complete recovery of the patient with no residual neurological deficit. Case report","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42599111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}