Brazilian Journal of Analytical Chemistry最新文献

{"title":"Development of a Modified Electrode with N-doped Carbon Dots for Electrochemical Determination of 17α-ethinylestradiol","authors":"B. Freire, Michael Monteiro, Jonatas de Oliveira S. Silva, José Fernando de Macedo, E. Sussuchi","doi":"10.30744/brjac.2179-3425.ar-44-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-44-2023","url":null,"abstract":"This work aims to develop a modified electrode with N-doped carbon dots (CPE/N-CD) for the voltammetric detection of 17α-ethinylestradiol (EE2) in solution. N-doped carbon dot nanoparticles (N-CD) were synthesized using citric acid and urea as the nitrogen source. The N-doped carbon dots were characterized by absorption and emission spectroscopy in the ultraviolet and visible regions, electronic absorption spectroscopy in the infrared region, and Raman Spectroscopy, which showed evidence of the formation of the material. Electrochemical analyses were conducted utilizing Differential Pulse Voltammetry (DPV). After optimization of electrochemical parameters, a calibration plot was produced for the sensor, demonstrating a linear range of 0.01 to 0.80 μmol L-1 (R2 = 0.9969). Additionally, the sensor exhibited a detection limit (LOD) of 0.59 nmol L-1 and a quantification limit (LOQ) of 2.00 nmol L-1. The studies on reproducibility and repeatability revealed RSDs of 1.63% and 3.61% respectively. The results obtained using CPE/N-CD indicate that the developed electrode exhibits excellent analytical performance, making it suitable for identifying and quantifying EE2 in solution.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49318629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Smartphone and Image Analysis in the Quantification of Vitamin C in Golden Berry (Physalis peruviana L.) Juice","authors":"Wilber Vilcapoma, Julio Pérez","doi":"10.30744/brjac.2179-3425.ar-34-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-34-2023","url":null,"abstract":"Conventional methods used to quantify vitamin C require expensive equipment; however, the image analysis method has proven to be effective in quantifying various bioactive compounds and could be useful for small industries due to its low cost. In this sense, the objective was to evaluate the use of a Smartphone and image analysis in the quantification of vitamin C in golden berries juice. Calibration curves were elaborated with ascorbic acid standards (2.5-20 mg L-1) and the Folin-Ciocalteu chromophore reagent (10%). Fifteen color parameters (analytical responses) were obtained from images obtained with a Smartphone and the ImageJ program of the colored samples using four backlight colors, to which a principal component analysis was applied using the integrated development environment for R, RStudio. Subsequently, one-way ANOVA and mean comparisons by Tukey's method (α = 0.05) were applied to the best-scoring analytical response's. Ultimately, the quantification of vitamin C in golden berry juice was performed using the image analysis method, which exhibited superior linearity and sensitivity (R2 = 0.9941 and m = 4.91). A comparative assessment was conducted against a spectrophotometric method utilizing the t-Student test for independent samples (α = 0.05), demonstrating no statistically significant difference between the two methods (p > 0.05).","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48842730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Brief Assessment of the Bioanalytical Methods by using LC-MS/MS for the Quantitation of Melatonin: A Potential Biomarker for Sleep-Related Disorders","authors":"Gorav Monga, Sravani Yerram, Shailaja Koppula, Rakesh Kumar, Sandeep Kumar","doi":"10.30744/brjac.2179-3425.rv-143-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.rv-143-2022","url":null,"abstract":"Due to its better sensitivity and selectivity, liquid chromatography with tandem mass spectrometry is the preferred choice for the quantification and identification of biomarkers, parent molecules/ metabolites in human saliva, plasma and urine etc. All such quantification methods for melatonin in biological matrices have been summarized. Melatonin is considered as a potential biomarker for circadian rhythm disturbance related disorders such as cancer, depression, Insomnia, etc. Accurate quantification of melatonin is very challenging and critically depends upon the reproducibility and ruggedness of the analytical method. LC-MS/MS technique is considered as the preferred method of analysis for melatonin as compared to immunoassays. Most of bioanalytical melatonin quantification methods consist of, extraction from the biological matrix analyzing by LC-MS/MS. Our review shows that LC-MS/MS is a rugged and dependable instrument for the robust and precise quantitation of Melatonin. This review compiles key elements like extraction procedure, linearity range, and chromatographic conditions.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44470906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation and Uncertainty Calculation of Rodenticide Analysis Methods in a Simulated Gastric Content Matrix – Uncertainty of Rodenticide Analysis Methods","authors":"Flávio Bezerra, Y. Sade, Jailton Damasceno, Renata Silva","doi":"10.30744/brjac.2179-3425.tn-22-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.tn-22-2023","url":null,"abstract":"Warfarin (WAR), brodifacoum (BDF) and bromadiolone (BDL) are compounds present in rodenticides, highly toxic to rats, humans and other animals. These compounds can be detected in complex matrices, such as stomach contents, by liquid chromatography techniques (HPLC) with mass spectrometry (MS) or fluorescence detection (FLD). However, no validated method showed determination of uncertainty in the quantification of these compounds. In this study, we compare the validation parameters of two analytical methods, HPLC FLD and ultra high performance liquid chromatography (UHPLC – MS), with uncertainty estimation for the three cited compounds. The results showed that UHPLC-MS outperformed HPLC FLD, however both methods were considered adequate for detection of WAR, BDF or BDL in samples of simulated human stomach contents, especially in cases of suspected contamination.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41473869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic Study for Determining As, Pb, Cd, and Se in Steel and Nickel Alloy Samples by GF AAS: Circumventing Matrix Interference with Extraction Based on Micellar Separation","authors":"Natália Viola, Brian Ferreira, L. Souza, M. Veiga","doi":"10.30744/brjac.2179-3425.ar-38-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-38-2023","url":null,"abstract":"This study proposes a matrix separation procedure based on micellar-mediated extraction (cloud point extraction - CPE) for determining As, Cd, Pb, and Se (potential contaminants) in nickel alloy and steel samples. Structural characterization and qualitative analysis of Ni alloy were conducted on the nickel alloy using scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM-EDS). After sample decomposition, ammonium o,o-diethyl dithiophosphate (DDTP) was used to complex the analytes and Triton X-114 as a non-ionic surfactant in CPE for matrix separation and extraction. Methanol acidified with 0.1 mol L-1 HNO3 was added to the surfactant-rich phase before the analytes determination by graphite furnace atomic absorption spectrometry (GF AAS). Parameters such as pH, complexing agent and surfactant concentrations, acid medium, complexation time, and type of diluent were evaluated. The obtained results indicated that the ratio DDTP:As was 3:1, DDTP:Cd and DDTP:Pb was 2:1, and DDTP:Se was 1:1. The enrichment factors were 6, 8, 14, and 13, and limits of detection were 1.5, 0.06, 0.31 and 0.27 µg g-1 for As, Cd, Pb, and Se, respectively. The method was applied for As, Cd, Pb, and Se determination in Inconel 625 nickel alloy and standard reference materials (AISI 4340 Steel - SRM® 361, AISI 94B17 Steel - SRM® 362, Chromium-Vanadium Steel - SRM® 363, and Nickel Alloy UNS - SRM® 864). Analyte recoveries lay above 88%, and relative standard deviations were lower than 5%. Application of cloud point extraction for matrix separation allowed the determination of low concentrations of As, Cd, Pb, and Se, constituting an environmentally friendly method.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45407081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Joanna Szpunar, a great chemical researcher internationally recognized, kindly spoke to BrJAC","authors":"J. Szpunar","doi":"10.30744/brjac.2179-3425.interview.jszpunar","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.interview.jszpunar","url":null,"abstract":"Joanna Szpunar graduated from the Warsaw University of Technology in 1986 and obtained her Ph.D. (1992) and D.Sc. (Habilitation) (2000) from the University of Warsaw. Since 1997, she works at the National Research Council of France (CNRS). In 2007, she obtained in Poland the title of professor of chemistry. She has broad experience in the field of mass spectrometry-based bio-inorganic speciation analysis and metallomics with a focus on the identification and quantification of trace element species in biological systems and in the chemistry of metal-biomolecule interactions. She is the co-author of a book and approximately 250 scientific publications in peer-reviewed international journals. Joanna Szpunar is a fellow of the Royal Society of Chemistry (UK) and has been, for many years, a member of the boards of Journal of Analytical Atomic Spectrometry' and Metallomics. She regularly gives invited lectures at international analytical chemistry meetings and was the chairperson of the European Winter Conference on Plasma Spectrochemistry (Kraków, Poland) in 2013. The investigations carried out under her supervision and/or with her active participation resulted in the identification of a number of molecular targets of metals in biological systems, as well as contribute to selenometabolomics and selenoproteomics studies in bacteria and plants. In recent years, her interests were broadened to the environmental fate of metal-containing nanoparticles and nanoplastics. She has supervised 12 Ph.D. theses and several post-doctoral fellows. Joanna Szpunar is the laureate of the 2013 Jerzy Fijalkowski Award of the Committee of Analytical Chemistry of the Polish Academy of Sciences and the 2017 European Award for Plasma Spectrochemistry. In recent years, Joanna Szpunar has been involved in collaboration with analytical chemists in Brazil, hosting graduate students from the University of Campinas and the Federal University of Pelotas in the laboratory at the Institute of Analytical Sciences (IPREM) in Pau afiliated with the CNRS, lecturing at the Federal University of Santa Maria and co-supervising a Ph.D. student with work carried out at the University of Pau and the Federal University of Pelotas.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41611986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Women in (Bio)Analytical Chemistry: Moving Towards Equity and Inclusion","authors":"M. D. da Veiga, M. D. G. Korn","doi":"10.30744/brjac.2179-3425.editorial.women.chem.n40","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.editorial.women.chem.n40","url":null,"abstract":"Women's participation in science has been an increasingly relevant and discussed topic in recent decades. Throughout history, women have faced numerous barriers and challenges to enter and stand out in this field of knowledge. However, despite the difficulties, women have contributed significantly to scientific advancement in all areas. In Brazil, the discussion about the participation of women in science has been highlighted in national events around analytical chemistry and academia. Thus, some changes have been implemented to minimize the differences discussed herein. Today we see more and more women engaged in science, holding prominent positions as researchers, professors, and leaders in their fields. They exist in many scientific disciplines, including physics, chemistry, biology, mathematics, engineering, and technology. In addition, women have also been involved in emerging and interdisciplinary areas such as computer science, artificial intelligence, and biotechnology. It is crucial to promote gender equality in chemistry by providing an inclusive environment and encouraging the active participation of women. This can be achieved by implementing policies that promote equal opportunities, encouraging female role models and mentors, supporting women chemists' training and professional development, and valuing and recognizing their contributions. Women's participation in chemistry is essential to scientific advancement in this field. We bring unique perspectives, creativity, and skills to address chemical challenges and contribute to a more comprehensive understanding of chemical processes and their practical applications. Gender diversity in chemistry drives innovation and promotes a more prosperous and inclusive scientific environment. It is essential to provide equal opportunities, support, and encouragement for women to enter and thrive in academic careers in the exact sciences, thus strengthening female representation in this field and boosting scientific excellence. In this context, the BrJAC journal worked on organizing an issue dedicated to women researchers in the field of (bio)analytical chemistry. The central idea is to celebrate those female researchers who have worked hard and dedicatedly in analytical chemistry. The interview was with Dr. Joanna Szpunar from the National Research Council of France (CNRS). She talked about her career and experience as a prominent researcher in the spectroanalytical chemistry field. The point of view was with Dr. Maria Valnice Boldrin Zanoni from UNESP, who excels in electroanalytical chemistry and shares a new perspective on artificial photosynthesis technology. The Letter written by Dr. Quezia Cass from UFSCar presented us with text on separation techniques highlighting affinity selection mass spectrometry. The issue will also feature three reviews, eight articles, and three technical notes, all led by women in (bio)analytical chemistry. It is imperative to thank all the reviewers who particip","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46597649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Adsorbent Potential of Biochar obtained by Pyrolysis to Remove Emerging Contaminants","authors":"Maria Alice Amaral, M. Fusinato, Michele da Cunha, Jean Paulo Coll, João Henrique Lacerda, P. S. Sanches Filho","doi":"10.30744/brjac.2179-3425.tn-31-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.tn-31-2023","url":null,"abstract":"Emerging contaminants (EC) can be harmful to human health and the environment, as many of these compounds are persistent, bioaccumulative, toxic and are not regulated. Drugs are part of this group, including hormones (estrone, β-estradiol) and sunscreens (oxybenzone and octocrylene) used in this study. The adsorption capacity of biochar generated from the pyrolysis of rice husks from the region of Pelotas-RS-Brazil was evaluated in this study. The objective was to characterize the biochar and evaluate its adsorbent potential in the removal of hormones and sunscreens. The characterization of biochar was carried out through surface area (BET), Zero charge point (ZPC), X-ray diffraction (XFR), fixed carbon, volatile, and ash. The effectiveness in removing ECs was evaluated by stirring rice husk pyrolysis biochar (RHPB) (0.2 g and 0.5 g) in synthetic aqueous solutions fortified with 10.0 µg L-1 of the analytes at 25 °C for 1 hour at 150 rpm. The RHPB was filtered off and the analytes were desorbed with acetone and dichloromethane (DCM) under ultrasound for 30 min. The extract was concentrated, derivatized by silylation, and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Biochar showed promising removal results, reaching values of 60.1% removal for β-estradiol, 59.4% for estrone, 44.6% for octocrylene and 37.4% for oxybenzone.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42267295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple Approach for Fe Determination in Beer by using Dispersive Liquid-Liquid Microextraction and Flame Atomic Absorption Spectrometry","authors":"Ma.Helen P. Seco, Eliana Larruscain, Jader Silveira, R. Picoloto, F. Duarte","doi":"10.30744/brjac.2179-3425.ar-18-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-18-2023","url":null,"abstract":"In this work, a new method for Fe extraction and preconcentration from beer by using dispersive liquid-liquid microextraction (DLLME) and determination by flame atomic absorption spectrometry (F AAS) was developed. Although in DLLME the use of an extraction solvent is mandatory (at least in theory), which is combined with a disperser solvent, in this work only the extraction solvent was used. For Fe extraction by DLLME, the type and volume of extraction solvent, sample volume, type of complexing agent, sample pH, salting out effect, and the number of washing cycles after extraction were evaluated. Thus, after optimization, 10 mL of beer, 75 µL of 1,2-dichlorobenzene of extraction solvent, 500 µL of 0.5% (w/v) pyrrolidine ammonium dithiocarbamate (APDC) as complexing agent were used. It is important to mention that pH adjustment, salt addition or washing cycles were no required. For Fe determination after DLLME, an external calibration curve was performed (R2 > 0.99) by direct extracts (reference solutions through DLLME) introduction in the conventional nebulization system of F AAS instrument. The accuracy evaluation was performed by results comparison with those obtained after microwave-assisted digestion (MAD) and Fe determination by inductively coupled plasma optical emission spectrometry (ICP-OES). The Fe concentration in the studied samples ranged from 59.7 to 68.2 µg L-1 and the limit of quantification for DLLME/F AAS method was 9.0 µg L-1. Finally, the proposed method DLLME/F AAS was applied for Fe determination in several beer samples showing simplicity, low operational costs, suitable accuracy (104 to 119%) and precision (better than 13%), opening the possibility for its use in routine analysis for Fe determination in beer.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41806955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Closed-Vessel Conductively Heated Digestion of Dry Dog Food for Spectrometric Determination of Essential Nutrients","authors":"Rayane Costa, J. V. Santiago, E. Ferreira, A. Virgilio, J. G. Gomes Neto","doi":"10.30744/brjac.2179-3425.ar-4-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-4-2023","url":null,"abstract":"The closed-vessel conductively heated digestion system (CHDS) was evaluated to digest dry dog foods for further determination of K, Na, Cu, Fe, Mn, and Zn by high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). The CHDS method was optimized using a fractional factorial design with five variables (HNO3 concentration, H2O2 volume, temperature, holding time, and pre-digestion time) at two levels. The accuracy of the CHDS procedure was checked by the analysis of reference materials from the National Institute of Standards and Technology (NIST SRM 1577b Bovine Liver and 2976 Mussel Tissue) and Brazilian Agricultural Research Corporation (Embrapa MR-E1002A Fish Food). Also, the digestion efficiencies were calculated from residual carbon contents (RCCs). The RCC and blank values in the CHDS digested samples were consistently low, which is suitable for determinations using ICP OES and ICP-MS techniques. For comparison, all samples were also digested by microwave-assisted digestion in closed vessels (MW-AD). Results for Na, K, Cu, Fe, Mn, and Zn determined in sample digests obtained by CHDS were not statistically different at a 95% confidence level from those observed for MW-AD. Limits of quantification (LOQ) calculated from digests in CHDS and MW-AD were comparable, and the values provided adequate limits for elemental determinations in dog foods. Data from mineral composition and moisture were employed in a clustering analysis (HCA) and the discrimination of the samples among different manufacturers and food for dogs at different life stages was possible.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41650589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}