Biomedical and environmental sciences : BES最新文献_第7页

A cell-based high-throughput screening assay for Farnesoid X receptor agonists. Farnesoid X受体激动剂的细胞高通量筛选试验。

Biomedical and environmental sciences : BES Pub Date : 2007-12-01

Zhi-Hui Zheng, Guo-Ping Lv, Shu-Yi Si, Yue-Sheng Dong, Bao-Hua Zhao, Hua Zhang, Jian-Gong He

{"title":"A cell-based high-throughput screening assay for Farnesoid X receptor agonists.","authors":"Zhi-Hui Zheng, Guo-Ping Lv, Shu-Yi Si, Yue-Sheng Dong, Bao-Hua Zhao, Hua Zhang, Jian-Gong He","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop a high-throughput screening assay for Farnesoid X receptor (FXR) agonists based on mammalian one-hybrid system (a chimera receptor gene system) for the purpose of identifying new lead compounds for dyslipidaemia drug from the chemical library.Methods: cDNA encoding the human FXR ligand binding domain (LBD) was amplified by RT-PCR from a human liver total mRNA and fused to the DNA binding domain (DBD) of yeast GAL4 of pBIND to construct a GAL4-FXR (LBD) chimera expression plasmid. Five copies of the GAL4 DNA binding site were synthesized and inserted into upstream of the SV40 promoter of pGL3-promoter vector to construct a reporter plasmid pG5-SV40 Luc. The assay was developed by transient co-transfection with pG5-SV40 Luc reporter plasmid and pBIND-FXR-LBD (189-472) chimera expression plasmid.Results: After optimization, CDCA, a FXR natural agonist, could induce expression of the luciferase gene in a dose-dependent manner, and had a signal/noise ratio of 10 and Z' factor value of 0.65.Conclusion: A stable and sensitive cell-based high-throughput screening model can be used in high-throughput screening for FXR agonists from the synthetic and natural compound library.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 6","pages":"465-9"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27327888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast evaluation of oxidative DNA damage by liquid chromatography-electrospray tandem mass spectrometry coupled with precision-cut rat liver slices. 液相色谱-电喷雾串联质谱联用精确切割大鼠肝片快速评价DNA氧化损伤。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Jiang Yue, Peng Wang, Ying-Hui Liu, Jun-Yu Wu, Jie Chen, Ren-Xiu Peng

{"title":"Fast evaluation of oxidative DNA damage by liquid chromatography-electrospray tandem mass spectrometry coupled with precision-cut rat liver slices.","authors":"Jiang Yue, Peng Wang, Ying-Hui Liu, Jun-Yu Wu, Jie Chen, Ren-Xiu Peng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish a fast and sensitive method for the detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid (INH) -induced oxidative DNA damage.Methods: Precision-cut liver slices (300 microm) were prepared from male rats, and incubated with INH (0.018 mol/L) for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37 degrees C. The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine (dG).Results: The limit of detection of 8-OHdG was 1 ng/mL (S/N=3) and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices (P<0.05).Conclusion: 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 5","pages":"386-91"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27217654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of anoxic sulfide biooxidation under various HRTs using artificial neural networks. 利用人工神经网络预测不同hrt下的缺氧硫化物生物氧化。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Qaisar Mahmood, Ping Zheng, Dong-Lei Wu, Xu-Sheng Wang, Hayat Yousaf, Ejaz Ul-Islam, Muhammad Jaffar Hassan, Ghulam Jilani, Muhammad Rashid Azim

{"title":"Prediction of anoxic sulfide biooxidation under various HRTs using artificial neural networks.","authors":"Qaisar Mahmood, Ping Zheng, Dong-Lei Wu, Xu-Sheng Wang, Hayat Yousaf, Ejaz Ul-Islam, Muhammad Jaffar Hassan, Ghulam Jilani, Muhammad Rashid Azim","doi":"","DOIUrl":"","url":null,"abstract":"Objective: During present investigation the data of a laboratory-scale anoxic sulfide oxidizing (ASO) reactor were used in a neural network system to predict its performance.Methods: Five uncorrelated components of the influent wastewater were used as the artificial neural network model input to predict the output of the effluent using back-propagation and general regression algorithms. The best prediction performance is achieved when the data are preprocessed using principal components analysis (PCA) before they are fed to a back propagated neural network.Results: Within the range of experimental conditions tested, it was concluded that the ANN model gave predictable results for nitrite removal from wastewater through ASO process. The model did not predict the formation of sulfate to an acceptable manner.Conclusion: Apart from experimentation, ANN model can help to simulate the results of such experiments in finding the best optimal choice for ASObased denitrification. Together with wastewater collection and the use of improved treatment systems and new technologies, better control of wastewater treatment plant (WTP) can lead to more effective maneuvers by its operators and, as a consequence, better effluent quality.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 5","pages":"398-403"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27217656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial remediation and optimization of oil polluted wetlands at Dalian Bay in China. 大连湾石油污染湿地微生物修复与优化研究

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Lei-Chang Huang, Shu-Hong Ye, Yu Zhang, Yao Olive Li, Xiang-Rong Wang, Dewen Ding

{"title":"Microbial remediation and optimization of oil polluted wetlands at Dalian Bay in China.","authors":"Lei-Chang Huang, Shu-Hong Ye, Yu Zhang, Yao Olive Li, Xiang-Rong Wang, Dewen Ding","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The wetland at Dalian Bay in the Northeast of China has been polluted by oil severely. The effect of various microbes and operation parameters on the bioremediation of oil-polluted wetlands at Dalian Bay was investigated and reported previously. In the study, other operation conditions related to the status of medium were investigated via statistical experimental design and analysis and a necessary information is involved to use micro-technology in the application.Methods: The method used involved the direct inoculation of selected bacteria, which were capable of degrading oil. The operation conditions were further optimized and evaluated by gravimetrical assay.Results: The optimal pH and temperature for the studied bacteria to degrade the existing oil pollutants were established as pH 8.0 and 27 degrees C. The mixed of various bacteria showed better results in terms of oil degradation than any single one. Among the selected four factors, disturbance, oxidant, nutrients, and biosurfactant, the former two contributed more impacts on the oil degradation in the early stage of process, while the latter two became the limiting factors in the late stage. Three sets of optimal conditions were obtained for each individual stage, but no one was suitable for the overall process.Conclusion: The study demonstrated the technical feasibility of using direct inoculation into the contaminated soil samples to remove oil pollutants. It suggested that the operation conditions should be monitored and adjusted during the different stages of bio-reactions in the process to achieve the best result of oil degradation.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 5","pages":"414-9"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27217659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A meta-analysis of beta-fibrinogen gene-455G/A polymorphism and plasma fibrinogen level in Chinese cerebral infarction patients. 中国脑梗死患者β -纤维蛋白原基因455g /A多态性与血浆纤维蛋白原水平的meta分析

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Xiao-Chao Chen, Ming-Tong Xu, Wu Zhou, Chun-Li Han, Wei-Qing Chen

{"title":"A meta-analysis of beta-fibrinogen gene-455G/A polymorphism and plasma fibrinogen level in Chinese cerebral infarction patients.","authors":"Xiao-Chao Chen, Ming-Tong Xu, Wu Zhou, Chun-Li Han, Wei-Qing Chen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the correlation between the beta-fibrinogen gene-455G/A polymorphism and cerebral infarction in Chinese population by means of meta-analysis.Methods: Genetic association studies on evaluating the beta-fibrinogen gene -455G/A polymorphism and cerebral infarction involving Chinese population published before December 2005 were collected from database of PubMed, EMBASE, and CNKI. All the data in literature were abstracted based on the defined selection criteria by two independent investigators. Publication bias was tested by funnel plot and the odd ratios of all studies were combined dependent on the result of heterogeneity test among the individual studies. The software Review Manager (Version 4.2) was used for meta-analysis.Results: Eleven studies including 1405 patients and 1600 controls met the selection criteria. There was no publication bias in 11 reviewed studies. Heterogeneity test of reviewed studies showed statistically significant differences (chi2=24.58, P=0.006) among the ORs of individual studies. The combined OR of 11 studies of susceptibility to cerebral infarction in -455A allele carriers compared with the -455G/G wild homozygotes was 1.33 (95%CI 1.04-1.71, P=0.02). In the patients with cerebral infarction in 6 studies, the summarized average plasma fibrinogen level of allele A carrier was 0.29 g/L (95%CI 0.14-0.44, P=0.0002) higher than that of -455G/G homozygous ones.Conclusions: beta-fibrinogen gene -455G/A polymorphism might contribute to susceptibility of cerebral infarction in Chinese population; allele A increases the individual susceptibility to the disease.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 5","pages":"366-72"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27218240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of 5-amino-2-chlorotoluene-4-sulfonic and chlorhydric acids from wastewater by weakly basic resin: equilibrium and kinetics. 弱碱性树脂去除废水中的5-氨基-2-氯-甲苯-4-磺酸和氯酸:平衡和动力学。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Chang-Hai Li

引用次数: 0

Synergetic inactivation of microorganisms in drinking water by short-term free chlorination and subsequent monochloramination. 短期游离氯化和随后的单氯胺化对饮用水中微生物的协同灭活作用。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Xiao-Jian Zhang, Chao Chen, Yun Wang

引用次数: 0

A time series analysis of outdoor air pollution and preterm birth in Shanghai, China. 上海市室外空气污染与早产的时间序列分析。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Li-Li Jiang, Yun-Hui Zhang, Gui-Xiang Song, Guo-Hai Chen, Bing-Heng Chen, Nai-Qing Zhao, Hai-Dong Kan

{"title":"A time series analysis of outdoor air pollution and preterm birth in Shanghai, China.","authors":"Li-Li Jiang, Yun-Hui Zhang, Gui-Xiang Song, Guo-Hai Chen, Bing-Heng Chen, Nai-Qing Zhao, Hai-Dong Kan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the relation between air pollution exposure and preterm birth in Shanghai, China.Methods: We examined the effect of ambient air pollution on preterm birth using time-series approach in Shanghai in 2004. This method can eliminate potential confounding by individual risk factors that do not change over a short period of time. Daily numbers of preterm births were obtained from the live birth database maintained by Shanghai Municipal Center of Disease Control and Prevention. We used the generalized additive model (GAM) with penalized splines to analyze the relation between preterm birth, air pollution, and covariates.Results: We observed a significant effect of outdoor air pollution only with 8-week exposure before preterm births. An increase of 10 microg/m3 of 8-week average PM10, SO2, NO2, and O3 corresponded to 4.42% (95%CI 1.60%, 7.25%), 11.89% (95%CI 6.69%, 17.09%), 5.43% (95%CI 1.78%, 9.08%), and 4.63% (95%CI 0.35%, 8.91%) increase of preterm birth. We did not find any significant acute effect of outdoor air pollution on preterm birth in the week before birth.Conclusion: Ambient air pollution may contribute to the risk of preterm birth in Shanghai. Our analyses also strengthen the rationale for further limiting air pollution level in the city.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 5","pages":"426-31"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27217661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reductive dechlorination of p-chlorophenol by nanoscale iron. 纳米级铁对氯酚的还原脱氯。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

Rong Cheng, Jian-Long Wang, Wei-Xian Zhang

引用次数: 0

Recovery of nitrogen and phosphorous as struvite from swine waste biogas digester effluent. 从猪废沼气池出水中以鸟粪石形式回收氮磷。

Biomedical and environmental sciences : BES Pub Date : 2007-10-01

P W Anton Perera, Zhi-Ying Han, Ying-Xu Chen, Wei-Xiang Wu

{"title":"Recovery of nitrogen and phosphorous as struvite from swine waste biogas digester effluent.","authors":"P W Anton Perera, Zhi-Ying Han, Ying-Xu Chen, Wei-Xiang Wu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the feasibility of nitrogen and phosphorus recovery from swine waste biogas digester effluent and the effects of pH and NH4+: Mg2+: PO4(3-) molar ratio on its precipitation.Methods: Precipitation experiments with swine waste biogas digester effluent were conducted at pH 7.5, 8.0, 8.5, and 9.0 together with NH4+: Mg2+: PO4(3-) molar ratios 1: 0.2: 0.08, 1: 1: 1, and 1: 1.5: 1.5. Chemical and X-ray diffraction (XRD) analysis were done to determine the composition of the precipitate.Results: The highest removal and recovery of NH4+ and PO4(3-) were achieved at pH 9.0 in each experiment. The elevation of pH to 9.0 alone could decrease the initial PO4(3-) concentration from 42 mg L(-1) to 4.7 mg L(-1) and 89.2% PO4(3-) recovery was achieved. The pH-molar ratio combination 9.0-1: 1.5: 1.5 effected 76.5% NH4+ and 68.5% PO4(3-) recovery. The molar ratio of 1: 1: 1 together with pH elevation to 9.0 was determined to be the optimum combination for both NH4+ and PO4(3-) removal as it recovered over 70% and 97% of the initial NH4+ and PO4(3-), respectively.Conclusions: Nitrogen and phosphorus can be recovered from biogas digester effluent as struvite.","PeriodicalId":9108,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"20 5","pages":"343-50"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27218237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0