Enliven. Journal of stem cell research & regenerative medicine最新文献

valuation of Safety and Preliminary Effectiveness of Point-of-Care Cell Processing System: An Observational Study 即时细胞处理系统的安全性和初步有效性评估:一项观察性研究

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-12-30 DOI: 10.33425/2639-9512.1031

Saniya Gupta, Manish Suthar, D. Sethi, Xiaochun Xu, Suhail Buk̲h̲ārī, H. Hegde, M. Sivilotti, K. Harris, Venkatesh Ponemone

引用次数: 0

A Holistic Approach to Managing Patients with Chronic Wounds No Disclosures 一个整体的方法来管理患者的慢性伤口没有披露

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-12-30 DOI: 10.4172/2325-9620-C5-020

Jassin Hamed

引用次数: 0

The Place of PRP and PRF Methods for Trophic Ulcers Treatment in Patients with Decompensation Stages of Varicose Veins in Combination with RFA PRP和PRF联合RFA治疗失代偿期静脉曲张患者营养溃疡的位置

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-12-30 DOI: 10.33425/2639-9512.1033

Goshchynsky V.B, L. O.B, M. Bo, Pyatnychka T.V, Pyatnychka O.Z

{"title":"The Place of PRP and PRF Methods for Trophic Ulcers Treatment in Patients with Decompensation Stages of Varicose Veins in Combination with RFA","authors":"Goshchynsky V.B, L. O.B, M. Bo, Pyatnychka T.V, Pyatnychka O.Z","doi":"10.33425/2639-9512.1033","DOIUrl":"https://doi.org/10.33425/2639-9512.1033","url":null,"abstract":"45 patients with varicose disease of the lower extremities at the stage of decompensation (C6 according to the CEAR classification) were under observation. Among them there were 34 women, 11 men, at the age of 45 ± 46 years old. The size of the trophic ulcers was from 2.5 × 1.5 to 8.5 × 7.5 cm, the duration of them was on average up to 8 months.The complex clinical, morphological, bacteriological examination of the trophic ulcers has been carried out to all patients treated. To objectively evaluate the dynamics of reparative processes in trophic ulcers, the MEASURE system was used, as well as the determination of the regenerativedegenerative index before and after the PRP, the PRF was made. For PRP and PRF, the plasma-enriched growth factors (PRGF®-ENDORET®) was prepared according to the protocol of the BTI Institute of Biotechnology (Victoria, Spain). Injections were performed on the periphery of the trophic ulcer. Then the surface of the ulcer was covered with a plasmа (fibrin) membrane enriched with growth factors. This made it possible to accelerate the appearance of granulation tissue and marginal epithelization in the trophic ulcer and significantly increase the number of cells responsible for reparative processes (macrophages, fibroblasts, polyblasts).","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89483043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mobilization of Stem Cells Using Plant Extracts 利用植物提取物动员干细胞

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-12-30 DOI: 10.33425/2639-9512.1030

G. Subramanyam, K HimakarReddy, M. Shaik

引用次数: 0

Transplantation of Human dgHPSCs Overexpressing Insulin and ERRγ can Efficiently Decrease the Glucose and HbA1c levels, Increase the Secretion of C-peptide and Repair the Complications of Coronary Heart Disease in T2D Patient (Case #1-A) 过表达胰岛素和ERRγ的人dgHPSCs移植可有效降低t2dm患者血糖和HbA1c水平，增加c肽分泌，修复冠心病并发症(病例#1-A)

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-12-30 DOI: 10.33425/2639-9512.1034

Taihua Wang, Xin Wang, Bailing Zhang, M. Fan, Xingqi Chen, Xiaohui Cui, Lina Zhao, Zhenzhen Yang, Xinyi Shi, Rongrong Li, Qingchang Fang, Xiaojuan Diao, Limin Zhang, Guoke Yang, Y. Meng, Gang Zhang

{"title":"Transplantation of Human dgHPSCs Overexpressing Insulin and ERRγ can Efficiently Decrease the Glucose and HbA1c levels, Increase the Secretion of C-peptide and Repair the Complications of Coronary Heart Disease in T2D Patient (Case #1-A)","authors":"Taihua Wang, Xin Wang, Bailing Zhang, M. Fan, Xingqi Chen, Xiaohui Cui, Lina Zhao, Zhenzhen Yang, Xinyi Shi, Rongrong Li, Qingchang Fang, Xiaojuan Diao, Limin Zhang, Guoke Yang, Y. Meng, Gang Zhang","doi":"10.33425/2639-9512.1034","DOIUrl":"https://doi.org/10.33425/2639-9512.1034","url":null,"abstract":"Daily injection of insulin is a widely used treatment for human diabetes. Yet, exogenous insulin injection cannot efficiently prevent the development of diabetes complications. Recently, we reported that the transplantation of directly-generated human pluripotent stem cells (dgHPSCs) overexpressing human insulin or estrogen-related receptor γ (ERRγ) gene can not only reduce the glucose levels of human diabetes patients, but also restore the function of human pancreatic β cells, and therefore with the potential to prevent the patients from developing diabetic complications. Here, we further reported that transplantation of dgHPSCs overexpressing insulin (INS) and ERRγ genes can significantly decrease the glucose and HbA1c levels and increase the secretion of C-peptide and repair the complication of coronary heart disease in type 2 diabetes (T2D) patient. Our data proved that this strategy can secrete high level insulin in vitro, increase the secretion of C-peptide (C-P) in vivo, decrease the patient’s glucose (GLU) and HbA1c levels, and repair the patient’s diabetic complications efficiently. Thus, our report is the first to prevent and reverse human diabetic complications and with the potential to completely cure human T2D.","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"31 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83602113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Patellofemoral Osteoarthritis: Treatment with Autologous Bone Marrow Mononuclear Cells and Arthroscopic Surgery, a Prospective Study 髌股骨关节炎:自体骨髓单核细胞治疗和关节镜手术，一项前瞻性研究

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-06-30 DOI: 10.33425/2639-9512.1026

M. Guimarães, Paula Alexandra da Graça Morais Rios, M. Ruiz, J. C. Noronha, Dalton Lopes Terra, J. F. Lana

{"title":"Patellofemoral Osteoarthritis: Treatment with Autologous Bone Marrow Mononuclear Cells and Arthroscopic Surgery, a Prospective Study","authors":"M. Guimarães, Paula Alexandra da Graça Morais Rios, M. Ruiz, J. C. Noronha, Dalton Lopes Terra, J. F. Lana","doi":"10.33425/2639-9512.1026","DOIUrl":"https://doi.org/10.33425/2639-9512.1026","url":null,"abstract":"Objective: The aim of this study was to evaluate the treatment using BMMCs and arthroscopy in PF OA through functional questionnaires and MRI evaluations in a two year follow up. The use of mononuclear cells derived from bone marrow (BMMCs) is under investigation, and in vitro and pre-clinic studies showed promisor results. In comparison to the mesenchymal stem cells (MSC), the effectiveness is lower, however the costs for manipulation and laboratory handling make it difficult to use in clinical practice. Design: This was a pilot, longitudinal and prospective trial and 8 patients with patellofemoral osteoarthritis who met the study criteria were included. All of the patients underwent arthroscopic debridement and received an injection of autologous BMMCs. Clinical outcomes were evaluated using SF-36 and the TLKSS questionnaire at baseline, one and two years after the procedure. Results: In this study, an improvement in all of the evaluated parameters of the questionnaire was verified even after two years following the applications. The functional score of TKLSS showed a significant improvement in one and two years in comparison to the baseline (p<0.001). A significant improvement in SF-36 for all of the domains (p<0.001) was also verified. In addition, an improvement in the MRI images of the patients was noticed, which indicates patellar cartilage recovery. Conclusion: The procedure of the arthroscopy and the application of BMMCs has proved promising results to reduce the signs of PF OA and ensure the patient satisfaction with a safe return to social life and sports practice. The completed questionnaire confirmed a clear improvement and a strong impact on the quality of life of the patients with the regeneration of their articular cartilage and restored subchondral bone. These results offer a wide perspective for future studies with the use of BMMC to treat articular diseases.","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81863751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antiarthritic Potential of Capparis Decidua (Forssk.) Edgew on FCA Induced Arthritis in Wistar Rats 冬青的抗关节炎作用FCA诱导Wistar大鼠关节炎的研究进展

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-06-30 DOI: 10.33425/2639-9512.1025

D. P. Kumar, Sharma Pramod Kumar, Kumar Sokindra

{"title":"Antiarthritic Potential of Capparis Decidua (Forssk.) Edgew on FCA Induced Arthritis in Wistar Rats","authors":"D. P. Kumar, Sharma Pramod Kumar, Kumar Sokindra","doi":"10.33425/2639-9512.1025","DOIUrl":"https://doi.org/10.33425/2639-9512.1025","url":null,"abstract":"The present investigation was designed to evaluate anti-arthritic potential of hydroalcoholic extract of leaves, root and stem of Capparis decidua. The anti-arthritic activity was evaluated using Freund’s complete adjuvants (FCA) induced arthritic models in Wistar rats. The arthritic study was carried out on basis of parameters including arthritic score, antinociceptive study, motor incoordination test, paw joint diameter, and biochemical parameters like serum AST (Aspartate aminotransferase), ALT (Alanine aminotransferase), ALP (Alkaline phosphatase) and total protein levels. Phytochemical analysis of C.decidua extract was done to assess the various phytoconstituents present in C.decidua. The results of C.decidua extract administration significantly (P<0.001) significantly decreased the arthritis which was evident with arthritis score, joint diameter, pain threshold. Also improvement in fall of time, and biochemical parameters suggested the antiarthritic role of C.decidua extract. The results indicate that hydroalcoholic C.decidua extract (100 mg/kg and 200mg/kg) showed a potent protective role against FCA induced arthritic rats which could be attributed to phytoconstituents present in C.decidua and its effect is comparable to the standard drug diclofenac sodium.","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85788665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Neural Stem Cells of Retinal Neuroepithelium Direct Retinal Ganglion Cell Axons Electrically: Galvanotropism in Embryonic Retina 视网膜神经上皮的神经干细胞直接视网膜神经节细胞轴突电性:胚胎视网膜的趋流性

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-06-30 DOI: 10.33425/2639-9512.1029

M. Yamashita

{"title":"Neural Stem Cells of Retinal Neuroepithelium Direct Retinal Ganglion Cell Axons Electrically: Galvanotropism in Embryonic Retina","authors":"M. Yamashita","doi":"10.33425/2639-9512.1029","DOIUrl":"https://doi.org/10.33425/2639-9512.1029","url":null,"abstract":"Growing axons are directed not only by chemical signals but also by electric fields in a process known as galvanotropism. Axons of embryonic neurons extend along the extracellular voltage gradient towards the cathode. During embryonic development neuroepithelial cells function as neural stem cells. The neuroepithelial cell has epithelial type sodium channels (ENaC), and the sodium transport via ENaC of retinal neuroepithelial cells produces extracellular positive direct current (DC) potentials within the retinal neuroepithelium. The amplitude of the positive DC potential is large at the periphery of the embryonic retina, and almost null at the ventral part of the optic cup, where the future optic disc is formed. Retinal ganglion cells are first born at the central part of the optic cup and they extend their axons along the endogenous voltage gradient; the disruption of the DC potential by blocking ENaC results in erroneous path finding of newborn retinal ganglion cell axons. Retinal ganglion cell axons can also be oriented by exogenous electric fields in vitro. Galvanotropism may be used to reform an optic nerve from the retinal ganglion cells that are generated from ES cells and iPS cells.","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86322273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting the Effects of Alteplase and DAPT on the Cleavage of C-Terminal Fragments and Amyloid-β Concentration 阿替普酶和DAPT对c端片段切割和淀粉样蛋白-β浓度影响的比较

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-06-30 DOI: 10.33425/2639-9512.1028

Arnav Gupta

{"title":"Contrasting the Effects of Alteplase and DAPT on the Cleavage of C-Terminal Fragments and Amyloid-β Concentration","authors":"Arnav Gupta","doi":"10.33425/2639-9512.1028","DOIUrl":"https://doi.org/10.33425/2639-9512.1028","url":null,"abstract":"Alzheimer’s disease is a very common type of dementia that triggers memory loss and impedes other important mental functions [1,2]. It is a neurodegenerative disorder which affects over 5 million people in the United States alone [13].In fact, it is the 6th leading cause of death in the United States, and on average, an American is diagnosed with Alzheimer’s disease every 66 seconds [1,3]. This can be a familial or sporadic disease which is primarily caused by the destruction of neurons which starts from the hippocampus and spreads throughout the brain (cerebellum is spared) [4]. The apoptosis of the countless neurons seems to be caused by a multitude of factors including amyloidbeta plaques, Tau tangles, and neuronal loss [1,2]. For the purposes of this investigation, there will be a primary focus on the amyloid-beta plaques because the buildup of neurotoxic plaques on the neurons seems to be a key factor in Alzheimer’s disease [1,2]. Enzymes called γ-secretase and β-secretase cleave a protein called an amyloid precursor protein (APP) to form these amyloid-beta peptides which can accumulate and form neurotoxic plaques [5,6]. Previous studies have found that DAPT (N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), a dipeptide analogue, is effective in inhibiting γ-secretase thus decreasing amyloid-beta concentration in the brain [6]. This study confirms the efficacy of DAPT in inhibiting γ-secretase, but also investigates the alternative inhibitory effects of drugs like Activase® rt-PA (alteplase), a tissue plasminogen activator typically used for treatment of stroke, and clonazepam (E64), a pill used to treat panic disorder and anxiety. Although the goal of this research was to observe the effects on both Aβ40 (40 amino acid amyloid-beta chain) and Aβ42 (42 amino acid amyloid-beta chain) production, only the effects of Aβ40 production were examined due to possible contamination in the Aβ42 tests.","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89238671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regenerative Therapy for Chronic Obstructive Pulmonary Disease, a Retrospective Study of Benefits and Safety 慢性阻塞性肺疾病的再生治疗，疗效和安全性的回顾性研究

Enliven. Journal of stem cell research & regenerative medicine Pub Date : 2018-06-30 DOI: 10.33425/2639-9512.1027

J. Coleman, Melissa Rubio

{"title":"Regenerative Therapy for Chronic Obstructive Pulmonary Disease, a Retrospective Study of Benefits and Safety","authors":"J. Coleman, Melissa Rubio","doi":"10.33425/2639-9512.1027","DOIUrl":"https://doi.org/10.33425/2639-9512.1027","url":null,"abstract":"This is a retrospective case series of 568 patients suffering from Chronic Obstructive Pulmonary Disease (COPD) treated with autologous cells and platelet rich plasma (PRP) during 2015. Our objectives were to determine any benefits of treatment as well as any risks to the patient. Quality of Life (QoL) was measured using the Clinical COPD Questionnaire (CCQ). There was a statistically significant improvement in the average self-reported improvement greater than 0.4, in all of our protocols. The proportion of patients experiencing significant improvement in QoL at the 6-month follow-up ranges from 68.2 to 80.0%, depending on the treatment protocol. This study shows that autologous cell therapy with PRP positively affected the QoL of 73% of patients treated using our protocols. Pulmonary function was also measured but no significant changes were observed in the Forced Expiratory Volume at 1 second (FEV1) or the FEV1 to Forced Vital Capacity (FVC) ratio: (FEV1/FVC) values from baseline. Neither age nor gender correlated with the QoL response. There were no complications related to the therapy noted in any patient. We postulate that the natural history of the disease in responding patients was positively changed during the duration of therapy and with little, if any, risk to the patient.","PeriodicalId":90820,"journal":{"name":"Enliven. Journal of stem cell research & regenerative medicine","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74371708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2