Virology reports最新文献_第2页

Rescue of duck-origin virulent Newcastle disease virus from cloned cDNA and stable expression of the red fluorescent protein 克隆cDNA拯救鸭源新城疫病毒及红色荧光蛋白的稳定表达

Virology reports Pub Date : 2016-12-01 DOI: 10.1016/j.virep.2016.10.002

Zhiqiang Duan , Xinqin Ji , Houqiang Xu , Jiafu Zhao , Haixu Xu , Shunlin Hu , Xiufan Liu

{"title":"Rescue of duck-origin virulent Newcastle disease virus from cloned cDNA and stable expression of the red fluorescent protein","authors":"Zhiqiang Duan , Xinqin Ji , Houqiang Xu , Jiafu Zhao , Haixu Xu , Shunlin Hu , Xiufan Liu","doi":"10.1016/j.virep.2016.10.002","DOIUrl":"10.1016/j.virep.2016.10.002","url":null,"abstract":"<div><p>Ducks are generally considered as potential reservoirs for different genotypes of Newcastle disease virus (NDV) and to be resistant even to velogenic NDV strains. However, outbreaks of highly virulent genotype VII NDV lethal to ducks have been frequently reported in China in recent years. But until now, the pathogenesis and potential vaccine of duck-origin genotype VII NDV are not known. In this study, a reverse genetics system using the prevalent high virulence genotype VIId isolate SS1 was constructed. Based on this system, a red fluorescent protein (RFP)-expressing virus was generated by inserting an additional transcription cassette coding for the RFP between the noncoding region of P and M genes. The rescue of the recombinant viruses was confirmed by western blotting, fluorescence microscopy and genetic marker detection. In addition, the replication kinetics, biological characteristics and pathogenicity of the rescued viruses were indistinguishable from the parental wild-type virus. Moreover, the recombinant virus rSS1-RFP could efficiently replicate in most of the duck tissues, especially in duck immune organs. The results obtained suggest that this reverse genetics system will provide a useful tool for the analysis of duck-origin NDV pathogenesis and dissemination, as well as preparation for novel vaccine vector or genotype-matched NDV attenuated vaccines used in ducks.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"6 ","pages":"Pages 97-103"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2016.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55311572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Modulation of LINE-1 retrotransposition by a human SAMHD1 polymorphism 人类SAMHD1多态性对LINE-1逆转录的调控

Virology reports Pub Date : 2016-12-01 DOI: 10.1016/j.virep.2016.06.001

Tommy E. White , Alberto Brandariz-Nuñez , Kyudong Han , Sara L. Sawyer , Baek Kim , Felipe Diaz-Griffero

引用次数: 9

Newly characterized arboviruses of northern Australia 澳大利亚北部新发现的虫媒病毒

Virology reports Pub Date : 2016-12-01 DOI: 10.1016/j.virep.2016.01.001

Bixing Huang , Richard Allcock , David Warrilow

引用次数: 9

Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia 澳大利亚石斑鱼betanodavirus RNA1和RNA2序列的分子分析

Virology reports Pub Date : 2016-12-01 DOI: 10.1016/j.virep.2016.05.001

Kalpana Agnihotri , Brad Pease , Roger Chong

{"title":"Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia","authors":"Kalpana Agnihotri , Brad Pease , Roger Chong","doi":"10.1016/j.virep.2016.05.001","DOIUrl":"10.1016/j.virep.2016.05.001","url":null,"abstract":"<div><p>Betanodavirus infections have a significant impact through direct losses and trade restrictions for aquaculture sectors in Australia. The giant grouper, <em>Epinephelus lanceolatus</em>, is a high-value, fast-growing species with significant aquaculture potential. With subacute to chronic mortalities reported from a commercial aquaculture facility in northern Queensland, the viral nervous necrosis in the affected fish was confirmed using a RT-qPCR followed by virus isolation using the SSN-1 cell line. The RNA1 and RNA2 segments were sequenced and nucleotide sequences were compared with betanodavirus sequences from GenBank. Phylogenetic analysis revealed that both these sequences clustered with sequences representing red spotted grouper nervous necrosis virus genotype and showed high sequence identity to virus sequences affecting other grouper species. This is the first report confirming infection by betanodavirus in <em>E. lanceolatus</em> from Australia with successful isolation of the virus in a cell culture system, and analysis of nearly full length RNA1 and RNA2 sequences.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"6 ","pages":"Pages 25-31"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2016.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55311921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

WITHDRAWN: Molecular and phylogenetic analysis of bovine papillomavirus type 1: First report in Iraqi cattle 撤回:牛乳头瘤病毒1型的分子和系统发育分析:首次在伊拉克牛中报告

Virology reports Pub Date : 2016-05-24 DOI: 10.1016/J.VIREP.2016.05.005

M. Hamad, Ahmed Majeed Al-Shammari, Shoni M. Odisho, N. Yaseen

引用次数: 1

Whole transcriptome sequencing of diseased elephant foot yam reveals complete genome sequence of Dasheen mosaic virus 病象脚山药的全转录组测序显示了Dasheen花叶病毒全基因组序列

Virology reports Pub Date : 2015-12-01 DOI: 10.1016/j.virep.2014.11.001

S. Kamala, T. Makeshkumar, J. Sreekumar, S.K. Chakrabarti

引用次数: 10

Full-length genome characterization and quasispecies distribution of hepatitis A virus isolates in China 中国甲型肝炎病毒分离株全基因组特征及准种分布

Virology reports Pub Date : 2015-12-01 DOI: 10.1016/j.virep.2015.03.001

Hao Wang, Xinying Wang, Jingyuan Cao, Yan Gao, Wenting Zhou, Shengli Bi

{"title":"Full-length genome characterization and quasispecies distribution of hepatitis A virus isolates in China","authors":"Hao Wang, Xinying Wang, Jingyuan Cao, Yan Gao, Wenting Zhou, Shengli Bi","doi":"10.1016/j.virep.2015.03.001","DOIUrl":"10.1016/j.virep.2015.03.001","url":null,"abstract":"<div><p>Hepatitis A virus (HAV) infection is the most common cause of acute viral hepatitis and has significant implications to public health worldwide. To characterize HAV strains circulating in China, five samples collected in different provinces from 2006–2009 were entirely sequenced. Phylogenetic analysis based on distinct segments showed that all five sequences belonged to subgenotype IA, but with slight differences in some fragments. No amino acid mutations were found at the known neutralizing epitope sites, and one unique substitution was identified near the immunodominant site. While no intertypic recombination was detected, intratypic recombination signals were found in the study. Molecular evolution analyses showed that the estimated mean substitution rate of genotype I worldwide was 3.27×10<sup>−4</sup> substitutions/site/year, and the time to the most recent common ancestor (tMRCA) was about 267years ago. The quasispecies distribution across the complete genome was also evaluated, and the high mutation frequency regions were mainly at the nonstructural protein coding sequences. This study contributed information on the genotype distribution, selection pressure, neutralizing epitope site mutations, recombination events and quasispecies distribution of HAV strains in China. The evolutionary status of genotype I worldwide was also analyzed, which will provide a reference for future HAV molecular epidemiology studies.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"5 ","pages":"Pages 29-46"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2015.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55311821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The origin of HTLV-1 in southern Bahia by phylogenetic, mtDNA and β-globin analysis 巴伊亚南部HTLV-1的系统发育、mtDNA和β-珠蛋白分析

Virology reports Pub Date : 2015-12-01 DOI: 10.1016/j.virep.2015.05.002

Milena Magalhães Aleluia , Marco Antônio Gomes Mello , Luiz Carlos Junior Alcântara , Filipe Ferreira Almeida Rego , Lucas Pereira de Souza Santos , Bernardo Galvão-Castro , Marilda de Souza Gonçalves , Túlio de Oliveira , Lauro Juliano Marin , Sandra Mara Bispo Sousa , Sandra Rocha Gadelha

{"title":"The origin of HTLV-1 in southern Bahia by phylogenetic, mtDNA and β-globin analysis","authors":"Milena Magalhães Aleluia , Marco Antônio Gomes Mello , Luiz Carlos Junior Alcântara , Filipe Ferreira Almeida Rego , Lucas Pereira de Souza Santos , Bernardo Galvão-Castro , Marilda de Souza Gonçalves , Túlio de Oliveira , Lauro Juliano Marin , Sandra Mara Bispo Sousa , Sandra Rocha Gadelha","doi":"10.1016/j.virep.2015.05.002","DOIUrl":"10.1016/j.virep.2015.05.002","url":null,"abstract":"<div><p>Different hypotheses have been elaborated to explain how the HTLV spread throughout the world. It has been proposed that the virus was introduced in Bahia, during the slave-trade period from the 16th century to 19th century. However, there is no information about the HTLV evolutionary history in southern Bahia. The phylogeny is fundamental in order to clarify its introduction and dispersion. The DNA of 29 samples was extracted, followed by nested-PCR assay for the LTR and DNA sequencing. These sequences were analyzed by phylogenic methods. The mtDNA ancestry markers and β<sup>A</sup>-globin haplotypes were analyzed by PCR/RFLP. In relation to HTLV subtyping, all samples were classified as cosmopolitan subtype and transcontinental subgroup. Results suggest an ancient post-Columbian introduction of HTLV-1a-A associated with the slave trade between the XVI and late XIX centuries in southern Bahia. As regards the ethnicity of HTLV-infected women, the haplotype characterization of β-globin gene and the mtDNA ethnicity of HTLV-infected women, we have detected a major African contribution, with a predominance of Benin and Bantu types. HTLV-1 infection is spread in Bahia and the point of origin was possibly Salvador.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"5 ","pages":"Pages 63-74"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2015.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55311870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Mutations within ICP4 acquired during in vitro attenuation do not alter virulence of recombinant Marek's disease viruses in vivo 在体外衰减过程中获得的ICP4突变不会改变重组马立克病病毒在体内的毒力

Virology reports Pub Date : 2015-12-01 DOI: 10.1016/j.virep.2014.11.002

Evin Hildebrandt , John R. Dunn , Masahiro Niikura , Hans H. Cheng

{"title":"Mutations within ICP4 acquired during in vitro attenuation do not alter virulence of recombinant Marek's disease viruses in vivo","authors":"Evin Hildebrandt , John R. Dunn , Masahiro Niikura , Hans H. Cheng","doi":"10.1016/j.virep.2014.11.002","DOIUrl":"10.1016/j.virep.2014.11.002","url":null,"abstract":"<div><p>Marek's disease (MD) is a T-cell lymphoma of chickens caused by the oncogenic Marek's disease virus (MDV). MD is primarily controlled by live-attenuated vaccines generated by repeated <em>in vitro</em> serial passage. Previous efforts to characterize attenuated MDVs identified numerous mutations, particularly a convergence of high-frequency mutations around amino acids 60–63 within ICP4 (RS1), therefore, ICP4 was considered a candidate gene deserving further characterization. Recombinant MDVs were generated containing a single Q63H mutation or double Q63H+S1630P mutations. Despite the repetitive nature of mutations within ICP4, neither recombinant virus decreased virulence, although one mutant reduced <em>in vivo</em> replication and failed to transmit horizontally. Our results indicate that these mutations are insufficient to reduce disease incidence in infected birds, and suggest that variants in ICP4 do not directly alter virulence, but rather may enhance MDV replication rates <em>in vitro</em>, offering an explanation for the widespread occurrence of ICP4 mutations in a variety of attenuated herpesviruses.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"5 ","pages":"Pages 10-18"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2014.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55311784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A novel codon-optimized SIV gag-pol immunogen for gene-based vaccination 一种新型密码子优化的SIV gag-pol免疫原用于基因免疫

Virology reports Pub Date : 2015-12-01 DOI: 10.1016/j.virep.2015.03.002

Catherine M. Crosby , Eric A. Weaver , Reeti Khare , Zenaido T. Camacho , Michael A. Barry

{"title":"A novel codon-optimized SIV gag-pol immunogen for gene-based vaccination","authors":"Catherine M. Crosby , Eric A. Weaver , Reeti Khare , Zenaido T. Camacho , Michael A. Barry","doi":"10.1016/j.virep.2015.03.002","DOIUrl":"10.1016/j.virep.2015.03.002","url":null,"abstract":"<div><p>Simian immunodeficiency virus (SIV) is a robust pathogen used in non-human primates to model HIV vaccines. SIV encodes a number of potential vaccine targets. By far the largest and most conserved protein target in SIV is gag-pol encodes many epitopes with potential to drive multivalent T cell responses. While it is an attractive antigen, pol is only translated after a frame shift from gag so that only 1 in 10 gag proteins include this larger antigen. The codon bias of native lentiviral genes are also mismatched with the abundance of tRNAs in mammalian cells resulting in poor expression of unmodified SIV genes. To provide a better SIV gag-pol immunogen for gene-based vaccination, we codon-optimized the full gag-pol sequence from SIVmac239. To increase pol expression, we artificially moved the pol sequence in frame to gag to bypass the need for a translational frame shift for its expression. Finally, we inserted four “self-cleaving” picornavirus sequences into gag p24, protease, reverse transcriptase, and into integrase to fragment the proteins for potentially better immune presentation. We demonstrate that these immunogens are well expressed <em>in vitro</em> and drive similar antibody and T cell responses with or without cleavage sequences.</p></div>","PeriodicalId":90762,"journal":{"name":"Virology reports","volume":"5 ","pages":"Pages 47-55"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.virep.2015.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55311839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2