Journal of biomolecular research & therapeutics最新文献_第2页

Molecular Mechanisms of Angiotensin-converting Enzyme Inhibitory Peptide LAP Activity on Apoptosis of Vascular Smooth Muscle 血管紧张素转换酶抑制肽LAP活性对血管平滑肌凋亡的分子机制研究

Journal of biomolecular research & therapeutics Pub Date : 2019-01-01 DOI: 10.35248/2167-7956.19.8.178

Yi Shen, Hong Fang, A. O. Cavdar, Chi Liu

{"title":"Molecular Mechanisms of Angiotensin-converting Enzyme Inhibitory Peptide LAP Activity on Apoptosis of Vascular Smooth Muscle","authors":"Yi Shen, Hong Fang, A. O. Cavdar, Chi Liu","doi":"10.35248/2167-7956.19.8.178","DOIUrl":"https://doi.org/10.35248/2167-7956.19.8.178","url":null,"abstract":"We observe the expression levels of apoptotic cells, Bcl-2/Bax and caspase-9 on abdominal aorta of spontaneously hypertensive rats (SHRs) to investigate impact and mechanisms of angiotensin converting enzyme inhibitory peptide LAP in apoptosis of vascular smooth muscle cell. A total of 20 male SHRs were studied to detect apoptotic cells and the expression of apoptosis-related proteins (Bcl-2, Bax, caspase-9). The index of apoptotic cells in LAP group was significantly lower compared to the control group. The expression of Bcl-2 in the LAP group was significantly higher than the control group. However, the levels of Bax and caspase-9 expression in the LAP group were significantly lower compared to the control group. The apoptosis index was negatively correlated with Bcl-2 and positively correlated with Bax/caspase-9. Similarly, the inflammation markers in LAP group were significantly lower than those in the control group. The expression of Ang II was significantly decreased after treating with LAP in the abdominal arteries. Angiotensin- converting- enzyme inhibition peptide LAP inhibited apoptosis of vascular smooth muscle cells through up-regulation of Bcl-2 and down-regulation of Bax and caspase-9 in SHRs.","PeriodicalId":90579,"journal":{"name":"Journal of biomolecular research & therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69995024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reevaluation of Antioxidative Strategies for Birth Defect Prevention in Diabetic Pregnancies. 重新评估预防糖尿病孕妇出生缺陷的抗氧化策略。

Journal of biomolecular research & therapeutics Pub Date : 2016-09-01 Epub Date: 2016-02-12 DOI: 10.4172/2167-7956.1000145

Zhiyong Zhao

{"title":"Reevaluation of Antioxidative Strategies for Birth Defect Prevention in Diabetic Pregnancies.","authors":"Zhiyong Zhao","doi":"10.4172/2167-7956.1000145","DOIUrl":"10.4172/2167-7956.1000145","url":null,"abstract":"Diabetes mellitus in early pregnancy is the most severe maternal disease that is counted for 10% of newborn infants with structural defects. With the rapid increases in the number of diabetic women in childbearing age, the birth defect rate is projected to elevate dramatically. Thus, prevention of embryonic malformations becomes an urgent task. Animal studies have revealed an involvement of oxidative stress in diabetic embryopathy and treatment with antioxidants can reduce embryonic abnormalities. However, the failure of clinical trials using free radical-scavenging antioxidants to alleviate oxidative stress-related diseases prompts researchers to reevaluate the strategy in birth defect prevention. Hyperglycemia also disturbs other intracellular homeostasis, generating aberrant conditions. Perturbed folding of newly synthesized proteins causes accumulation of unfolded and misfolded proteins in the lumen of the endoplasmic reticulum (ER). The ER under the stress activates signaling cascades, known as unfolded protein response, to suppress cell mitosis and/or trigger apoptosis. ER stress can be ameliorated by chemical chaperones, which promote protein folding. Hyperglycemia also stimulates the expression of nitric oxide (NO) synthase 2 (NOS2) to produce high levels of NO and reactive nitrogen species and augment protein nitrosylation and nitration, resulting in nitrosative stress. Inhibition of NOS2 using inhibitors has been demonstrated to reduce embryonic malformations in diabetic animals. Therefore, targeting ER and nitrosative stress conditions using specific agents to prevent birth defects in diabetic pregnancies warrant further investigations. Simultaneously targeting multiple stress conditions using combined agents is a potentially effective and feasible approach.","PeriodicalId":90579,"journal":{"name":"Journal of biomolecular research & therapeutics","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35428406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA biomarkers for early detection of embryonic malformations in pregnancy. 早期检测妊娠期胚胎畸形的MicroRNA生物标志物。

Journal of biomolecular research & therapeutics Pub Date : 2014-12-01 DOI: 10.4172/2167-7956.1000119

Xuezheng Li, Zhiyong Zhao

引用次数: 6

Delivery of Polymeric Nanoparticles to Target Vascular Diseases. 靶向血管疾病的高分子纳米颗粒递送

Journal of biomolecular research & therapeutics Pub Date : 2014-01-01 DOI: 10.4172/2167-7956.s1-001

Edward Agyare, Karunyna Kandimalla

{"title":"Delivery of Polymeric Nanoparticles to Target Vascular Diseases.","authors":"Edward Agyare, Karunyna Kandimalla","doi":"10.4172/2167-7956.s1-001","DOIUrl":"https://doi.org/10.4172/2167-7956.s1-001","url":null,"abstract":"Current advances in nanotechnology have paved the way for the early detection, prevention and treatment of various diseases such as vascular disorders and cancer. These advances have provided novel approaches or modalities of incorporating or adsorbing therapeutic, biosensor and targeting agents into/on nanoparticles. With significant progress, nanomedicine for vascular therapy has shown significant advantages over traditional medicine because of its ability to selectively target the disease site and reduce adverse side effects. Targeted delivery of nanoparticles to vascular endothelial cells or the vascular wall provides an effective and more efficient way for early detection and/or treatment of vascular diseases such as atherosclerosis, thrombosis and Cerebrovascular Amyloid Angiopathy (CAA). Clinical applications of biocompatible and biodegradable polymers in areas such as vascular graft, implantable drug delivery, stent devices and tissue engineering scaffolds have advanced the candidature of polymers as potential nano-carriers for vascular-targeted delivery of diagnostic agents and drugs. This review focuses on the basic aspects of the vasculature and its associated diseases and relates them to polymeric nanoparticle-based strategies for targeting therapeutic agents to diseased vascular site.","PeriodicalId":90579,"journal":{"name":"Journal of biomolecular research & therapeutics","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2167-7956.s1-001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33263836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

The Critical Role of Calpain in Cell Proliferation. 钙蛋白酶在细胞增殖中的关键作用。

Journal of biomolecular research & therapeutics Pub Date : 2014-01-01 DOI: 10.4172/2167-7956.1000112

Laszlo Kovacs, Yunchao Su

引用次数: 16

Modulation of Transcription mediated by the Vitamin D Receptor and the Peroxisome Proliferator-Activated Receptor δ in the presence of GW0742 analogs. GW0742类似物存在下维生素D受体和过氧化物酶体增殖物激活受体δ介导的转录调节

Journal of biomolecular research & therapeutics Pub Date : 2014-01-01 DOI: 10.4172/2167-7956.1000111

Kelly Teske, Premchendar Nandhikonda, Jonathan W Bogart, Belaynesh Feleke, Preetpal Sidhu, Nina Yuan, Joshua Preston, Robin Goy, Leggy A Arnold

引用次数: 6