Kittiphong Putkhao, Anthony W S Chan, Yuksel Agca, Rangsun Parnpai
{"title":"Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.","authors":"Kittiphong Putkhao, Anthony W S Chan, Yuksel Agca, Rangsun Parnpai","doi":"10.4172/2168-9849.1000116","DOIUrl":"10.4172/2168-9849.1000116","url":null,"abstract":"<p><p>The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (<i>Macaca mulatta</i>) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic Huntington's disease (HD) rhesus monkey. The results showed no post-thaw motility difference among the freezing extender tested (P>0.05). However, sperm membrane integrity in TEST and TTE were significantly better than in TRIS extender (P<0.05). TEST was chosen for HD rhesus monkey sperm cryopreservation. The results showed that post-thawed HD sperm motility and viability was not different compared with WT control group (P>0.05). The present study demonstrates that TEST and TTE were excellent extenders and suitable for rhesus monkey sperm cryopreservation and no detectible differences of post-thaw sperm motility and viability between HD and WT rhesus monkeys resulted from TEST extender.</p>","PeriodicalId":90471,"journal":{"name":"Cloning & transgenesis","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243968/pdf/nihms-589426.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32843230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}