Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.

Cloning & transgenesis Pub Date : 2013-01-01 DOI:10.4172/2168-9849.1000116

Kittiphong Putkhao, Anthony W S Chan, Yuksel Agca, Rangsun Parnpai

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Abstract

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic Huntington's disease (HD) rhesus monkey. The results showed no post-thaw motility difference among the freezing extender tested (P>0.05). However, sperm membrane integrity in TEST and TTE were significantly better than in TRIS extender (P<0.05). TEST was chosen for HD rhesus monkey sperm cryopreservation. The results showed that post-thawed HD sperm motility and viability was not different compared with WT control group (P>0.05). The present study demonstrates that TEST and TTE were excellent extenders and suitable for rhesus monkey sperm cryopreservation and no detectible differences of post-thaw sperm motility and viability between HD and WT rhesus monkeys resulted from TEST extender.

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转基因亨廷顿氏病猕猴精子的冷冻保存--病例报告。

研究人员测试了三种不同精液冷冻扩展剂（柠檬酸三锂盐（Tris-citrate，TRIS）、TEST和Tes-Tris-Egg yolk（TTE））中的甘油对野生型恒河猴（Macaca mulatta）精子冷冻保存的保护作用。对精子的运动性和存活率进行了检测，以评估冷冻解冻精子的完整性，并选择了最佳扩展剂来冷冻保存转基因亨廷顿氏病（HD）恒河猴的精子。结果表明，所测试的冷冻扩展剂在解冻后的运动性方面没有差异（P>0.05）。然而，TEST 和 TTE 的精子膜完整性明显优于 TRIS 冷冻延长剂（P0.05）。本研究表明，TEST 和 TTE 是优良的冷冻延伸剂，适用于恒河猴精子的冷冻保存。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cloning & transgenesis

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