Bionatura最新文献

{"title":"Association of serum level and gene expression of IL-25 in a sample of Iraqi asthmatic patients","authors":"Basima Q. Hasan ALSaadi, Mayada Hussain Ali","doi":"10.21931/rb/2023.08.04.84","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.84","url":null,"abstract":"Asthma is an increasingly common disease characterized by allergic airway inflammation, hyperresponsiveness, mucus overproduction, and peribronchial fibrosis. The epithelial cytokines IL25 have been implicated in asthma pathogenesis because they promote Th2-type cytokine synthesis. The current study aimed to assess IL-25 expression and measure the serum level of this cytokine in asthmatic Iraqi patients. The study was conducted on sixty (60) asthmatic patients (32 female and28 male) and thirty as apparently healthy subjects (control) (16 female and 14 male) with ages ranging from 15-60 years recruited Alzahra’a Center for Asthma and Allergy in Baghdad during the period between November /2021 to March /2022. Written informed consent was obtained from all patients and a healthy control group.\u0000Moreover, the study design was approved by the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies/ University of Baghdad. All patients were diagnosed according to clinical examination by a chest physician and selected according to the global initiative of asthma criteria. The healthy control group are non-smoking subjects with no history of obstructive lung disease or atopy. The RNA was extracted from the whole blood of asthma patients and healthy control without Trizol subjects using the protocol in Easy Pure t® blood RNA Kit (ER-401). The acceptable purity of asthmatic patients and control healthy group RNA ranges between 1.84 and 1.99. IL-25 gene expression showed the Ct of asthma patient group (25.179) and control group (27.393), and the2-ΔΔCt of asthma patient group (288.81) and control group (58.48) and the fold of gene expression was statistically significantly (P≤0.01) the calculation of the gene expression it was significant in the patient group than control group4.938 times with ratio (4.938:1) . ELISA estimated IL-25concentration, the results were statistically significant (P≤0.001), the level of IL-25 in asthma patient group (29.30± 5.107317pg/ml) while the level of IL-23 in Control group (15.16±0.085248pg/ml).\u0000Keywords: IL-25, gene expression, Elisa, Asthma","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"92 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138999441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological, serological and molecular characterization of Potato virus Y strains isolated from potato (Solanum tuberosum L.)","authors":"Dena Zuheer, Hameed Ali","doi":"10.21931/rb/2023.08.04.60","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.60","url":null,"abstract":"Viral diseases are among the most critical determinants of potato production in many parts of the world, and this is reflected in the importance of developing early diagnosis methods to detect these diseases in the fields. The study showed that potato fields in Nineveh Province are infected with several strains of Potato Y virus, depending on the symptoms of potato or indicator plants and serological tests. For the first time locally in Nineveh Province and at the level of Iraq, using a single molecular test and one step, it was possible to distinguish between the ancient parental strains (PVYº and PVYN), as well as between the PVYNTNT hybrid strain resulting from recombination between the genome of the parental strains when using the pairs of primers N-868-7-F + N -9236-R, which yielded a genome segment of DNA with a length of 441 bp. The emergence of such a new strain in the tested samples in the potato fields in Nineveh province infected with PVY may have arisen from mixed infections.\u0000 \u0000Keywords: PVY strains; PVYN; RT-PCR; Solanum tuberosum L.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"45 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138995722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction Total Digestible Nutrient value of forage and feedstuffs from their chemical characteristics","authors":"F. Sarubbi, G. Auriemma, Raffaele Pappalardo, Giuseppe Grazioli","doi":"10.21931/rb/2023.08.04.23","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.23","url":null,"abstract":"Total Digestible Nutrient Value of forage and concentrate and nutritional characteristics and develop a prediction equation using the chemical composition variables as predictors. Nutrient chemical characteristics data were obtained from 278 forage and 87 feedstuffs. The data included dry and organic matter, crude protein, ether extract, ash, fiber composition, and non-fiber Carbohydrate. Stepwise regression was used to eliminate variables that did not influence variation in the model and used 0.05 as the critical probability level.\u0000 \u0000Data were then randomly divided into two parts; two-thirds of the data was used to estimate the Total Digestible Nutrient, whereas the remaining part was used to validate the estimated Total Digestible Nutrient and was analyzed by multiple linear regressions. Total Digestible Nutrient in forage was negatively correlated with Ether Extract, Acid Detergent Lignin, and Non-fibre Carbohydrate (P<0.01) but positively correlated with Crude Protein (P<0.01), ash, Neutral Detergent Fibre, and Acid Detergent Fiber. Total Digestible Nutrient in feedstuffs was negatively correlated with NFC (P<0.01) but positively correlated with Neutral Detergent Fibre (P<0.01), Acid Detergent Lignin (P<0.01), Ether Extract (P<0.01), Crude Protein (P<0.01), ash, and Acid Detergent Fiber (P<0.01). The results show that the Total Digestible Nutrient content can be accurately estimated starting from the chemical composition.\u0000 \u0000Keywords: total digestible nutrient; forage; concentrate; feed analysis.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"77 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138996167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of hydrostatic pressure on some quality properties in Iraqi rams and calves’ meats","authors":"Rabeea Mahmood, Anwer Mhannawee, Esraa Mohsen","doi":"10.21931/rb/2023.08.04.74","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.74","url":null,"abstract":"Two experiments have been conducted in this research to determine the effects of hydrostatic pressure on Iraqi rams and calves’ meats. (One experiment for each kind of meat), Four treatments were designed for each experiment: control with no hydrostatic pressure, T1 with 100 bar exposed pressure, T2 with 200 bar and T3 with 300 bar. The pressures were generated and handled via a device designed and manufactured for this goal. The studied properties were water holding capacity WHC, thaw loss, fragmentation index FI, myofibril fragmentation index MFI, protein solubility and shear force. All these properties are strongly related to meat tenderness. All studied properties were affected significantly by applying hydrostatic pressure on meat samples to rams and calves’ meat samples. Therefore, exposing the meat to hydrostatic pressure enhances its tenderness-related properties.\u0000 \u0000Keywords: hydrostatic pressure; tenderness; Protein solubility.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"213 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138997064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and enzymatic activity of some fungi isolated from agricultural soil","authors":"Nemat A. Muhsen, Mustafa A. Al-Dossary","doi":"10.21931/rb/2023.08.04.31","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.31","url":null,"abstract":"Fungi are one of the most important groups of microorganisms in the environment, and due to their ability to produce several types of enzymes, they play an essential role in the environment. During this study, fourteen species of fungi were isolated from fifteen soil samples collected from several agricultural areas in Basrah Governorate, southern Iraq, and their enzymatic activity was tested for four extracellular enzymes (cellulase C, laccase La, lipase Lp, and manganese peroxidase Mp) on specific solid media. The isolated fungi showed good enzymatic activity, in which 12 fungal species can secret manganese peroxidase, 11 can secret cellulase, 9 can secret lipase, and five can secret laccase enzyme. Aspergillus candidus and A. versicolor showed a positive detection for all enzymes, Cladosporium showed positive detection for C, Lp, and Mp, while Mucor sp. showed negative detection for all enzymes.\u0000Keywords: Enzymatic activity, Fungal diversity, soil.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"59 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138999335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Fungal Species from the Insect Pest Callosobruchus maculatus (F.)","authors":"Noor Akmoosh, Ekhlas Al-Shareefi, Kawther Mohammed Ali","doi":"10.21931/rb/2023.08.04.41","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.41","url":null,"abstract":"Cowpea seed beetle Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. The current research aimed to isolate the fungi associated with adults of C. maculatus (F.) (Coleoptera: Bruchidae). The present study aimed to isolate and identify fungi associated with adult insects and evaluate their potential as biocontrol agents against the cowpea beetle, C. maculatus. In this study, we collected C. maculatus adults from the Babylon local market and five regions (Hilah, Mahaweel, Al-Mashrou', Al-Haswa and Al-Musayyab) with no history of insecticide exposure. A potato dextrose agar medium was used to isolate the fungi attached to the surfaces of adult beetles. By Morphological and microscopic examination, Molecular identification and Sequencing analysis, Twenty-seven genera of fungi were isolated and identified from adult C. maculatus, including Aspergillus, Penicillium, Candida spp, white mycelium, Curvularia, Cladosporium, Chaetomium, Stachybotrys, Rhizopus, Drechslera, Scopulariopsis, Paecilomyces, Mucor, Geotrichum candidum, Streptomyces, Sarocladium, Beauveria bassiana, Talaromyces varians, Sporothrix flocculasa, Pseudozyma flocculasa, and Isaria fumosorosea. The molecular weights of the PCR products for the fungi isolates ranged from 650-700 bp, and the amplified ITS1-5.8S-ITS4 rDNA region of indigenous entomopathogenic fungal isolates showed a high sequence similarity (99-100%) with B. bassiana and M. anisopliae deposited in the NCBIGenebank. The phylogenetic tree analysis of the ITS region sequences showed a high degree of similarity between the isolates under study, ranging from 96.5-100. However, there were differences in the lines among clades, indicating genetic variation possibly due to mating or mutations in different environments. Our study suggests that the storage pest, C. maculatus, would be essential in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored Cowpea.\u0000Keywords: Callosobruchus maculatus, Isolation, Fungi","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"98 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138999750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The histomorphological appearance of the testes in Alloxan-induced diabetic rabbits","authors":"Hasanain A. Emran, Khyreia Habeeb, Abeer Majeed","doi":"10.21931/rb/2023.08.04.19","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.19","url":null,"abstract":"This study assessed the histomorphological changes in testicular tissues due to the experimental diabetes mellitus (DM) generated in rabbits with Alloxan. Twenty-four adult male rabbits were used and allocated randomly into two equal groups (n=12 of each). The first group served as a control group and received normal saline only. The second group (diabetic group) was injected with Alloxan at a 100mg/kg IP dose. The serum glucose level was estimated at (0 min), 3 days and then (1,2 and 3 months), by aspiration of a blood sample from the jugular vein mixed with anticoagulant (EDTA) to obtain serum. The testosterone hormone was assayed from the serum obtained by centrifugation of blood samples using a special kit. Testicular biopsies were harvested through surgical castration at (1,2, and 3 months) post-administration of Alloxan. Results displayed a marked increase in glucose levels in the diabetic group starting from day three till the end of the experiment, with high differences between the two groups.\u0000 \u0000Moreover, testosterone levels significantly decreased in the diabetic group compared to the control group. Our histomorphological examination confirmed that Alloxan leads to testicular damage in rabbits, represented by testicular atrophy, necrosis focusing in spermatogenic cells and separation of germinal epithelium. In conclusion, the injection of Alloxan succeeded in inducing (DM) with clear alteration in studied parameters.\u0000 \u0000. \u0000 \u0000Key Words: Biochemical, Testicular Parameters, Alloxan, Rabbits.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138999998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the source of the plant part and plant growth regulators on the establishment and multiplication of vegetative branches of Rosmarinus officinalis L. in vitro","authors":"Al-Hasan Nassrullah, S. Almukhtar","doi":"10.21931/rb/2023.08.04.87","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.87","url":null,"abstract":"This search was carried out in the plant tissue culture laboratory at the College of Agriculture at the University of Kerbala from 2021 to 2022. The study included the use of in vitro technology in the use of plant parts and different combinations in the emergence and multiplication of farms vegetative branches of rosemary, the study was carried out in two stages after performing the sterilization process: the first included the emergence of vegetative farms by planting the growing tops and side nodes on the MS nutrient medium with different concentrations (0 , 1, 2, 3 mg.L-1) BA, and the second stage was carried out by cultivating the growths resulting from the nodes grown in the previous stage on the (MS) media, that was prepared with various concentrations (0, 1, 2, 3 mg.L-1) BA and ( 0, 0.1, 0.2, 0.4mg.L-1) NAA, The results of the study showed the superiority of the apical, as it achieved a response rate of 57.5% compared to the lateral shoots, which recorded a response rate of 37.5%, and the con. of 1 mg.l-1 BA was superior and achieved a response rate of 80% comparison with the neutral treat that earn a response rate It reached 15%, and the results showed that the same concentration of benzyl adenine at a concentration of 2 mg.l-1 achieved of higher average in the number and length of branches (4.74 branches.plantlet-1, 4.12 cm), while the concentration achieved 3 mg .l- 1 of it had the higher rate of leaves number and the Fresh and Dry weight of the branches (15.24 leaves.plantlet-1, 2986 mg and 1823 mg), respectively, comparison with the neutral treat that achieved the lower average, and the concentration 0.2 mg.L-1 NAA exceeded in achieving The highest average number of branches, leaves, fresh &dry weight of branches was (4.16 branches.plantlet-1 and 14.21 leaves.plantlet-1, 2606 mg and 1594 mg) respectively, while the concentration exceeded 0.4 mg.L-1 NAA in achieving the highest rate The length of the branches reached 3.74 cm. All experiments were carried out using CRD (utterly randomized design).\u0000Keywords: explants type ; plant growth regulators ; micro propagation ; rosemary ; tissue culture","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"16 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Butterflies associated with Brassicaceae crops in Basra, Iraq","authors":"Faisal Nasser Jaber, Aqeel Alyousuf, H. H. Al-Saffar","doi":"10.21931/rb/2023.08.04.62","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.62","url":null,"abstract":"The study aimed to investigate the seasonal presence and characterization of the group of butterflies (abundance, richness and biodiversity) in the agroecosystem of Brassicaceae plants in the Basra province. Two different ecosystem sites (Karmat Ali at the sedimentary habitat and Al-Zubair region at the desert habitat) were sampled during the growing season of 2020/2021. There are 823 and 507 individuals at the Karmat Ali and Al-Zubair sites, respectively; the specimens consist of 9 butterflies belonging to four families. The most abundant family was Nymphalidae (60%), followed by Lycainidae (28%) and Pieridae (9%), while Hesperiidae recorded the lowest relative abundance of 3%. The highest butterfly population was recorded for Vanessa Carudi, while Pieris rapae had the lowest density. The results showed differences in abundance, species richness, and Diversity of butterflies at the sites. The highest values of Diversity and richness were at Karmat Ali, compared to the Al-Zubair region. The study of butterflies' abundance and biodiversity indices indicated that environmental factors and the polyculture plantations support the butterfly population in agricultural habitats.\u0000 \u0000Keywords: Basra; Biodiversity; Brassicaceae; Butterflies; Shannon index; Richness.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"13 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"La Innovación Tecnológica y su Impacto en la Educación Superior Technological Innovation and its Impact on Higher Education","authors":"Hernán Arturo Rojas Sánchez, Silvia Rosa Pacheco Mendoza, Carlos Napoleón Ribadeneira Zapata, Isidro Favián Bayas Morejón, Jorge Alexander Briceño Carrasquel, José Luis Gaibor Becerra, Michael Xavier Hachi Pazmiño, Jhoselyn Deyalit Guarnizo Ordóñez, Gustavo Javier Vega Romero","doi":"10.21931/rb/2023.08.04.1","DOIUrl":"https://doi.org/10.21931/rb/2023.08.04.1","url":null,"abstract":"Los tiempos postpandémicos han demostrado que la investigación científica es el método que permite generar soluciones eficaces a problemas urgentes y complejos. La ciencia tiene el poder de mostrar el camino hacia un futuro sostenible, saludable y equitativo.\u0000 \u0000 \u0000En este sentido, es importante la organización de eventos de difusión científica, puesto que son escenarios destinados al intercambio de ideas, presentación de avances y colaboración entre expertos de renombre de las diferentes disciplinas del conocimiento1.\u0000 \u0000 \u0000La Universidad Estatal de Bolívar (UEB) desempeña un papel importante como ente generador de espacios de articulación entre las funciones sustantivas de docencia, investigación y vinculación. Todo ello, enfocado en presentar alternativas para la resolución de los problemas que aquejan a la sociedad en el marco de la pertinencia y los fines institucionales.\u0000 \u0000 \u0000El Congreso Internacional de Ciencia, Tecnología, Innovación y Emprendimiento organizado por la Universidad Estatal de Bolívar, ha forjado su propia historia desde su primera edición, que fue ideado, organizado y llevado a cabo los días 10 y 11 de julio de 2013. Este acontecimiento se ha convertido en un hito dentro de la historia institucional y ha ido evolucionando conforme a la realidad dinámica del entorno social, académico y científico.","PeriodicalId":9036,"journal":{"name":"Bionatura","volume":"286 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138997085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}