Biological Communications最新文献

{"title":"Diversity and distribution patterns of antennal sensilla in Hydropsychidae (Insecta, Trichoptera)","authors":"K. A. Abu Diiak, M. Valuyskiy, S. Melnitsky, V. Ivanov","doi":"10.21638/spbu03.2021.403","DOIUrl":"https://doi.org/10.21638/spbu03.2021.403","url":null,"abstract":"Structure and distribution of antennal sensilla were studied in males of 19 species of the caddisfly family Hydropsychidae by using scanning electron microscopy (SEM). Eleven types of sensilla were found: long trichoid, chaetoid, thick chaetoid, curved trichoid, coronary, basiconic, styloconic and four types of pseudoplacoid sensilla (mushroom-like, auricillic, ribbed, and T-shaped). Thick chaetoid, ribbed pseudoplacoid, and T-shaped pseudoplacoid sensilla were found only in Macronematinae. The great diversity of pseudoplacoid sensilla originated from a mushroom-like type, which also has a variable structure. Basal flagellomeres in the majority of studied species are equipped with ventrally positioned sensory fields of curved trichoid sensilla. In contrast to Arctopsychinae and Hydropsychinae, the increased number of these sensilla in the fields was noted for Diplectroninae and Smicrideinae. Most Macronematinae show a reduction of sensory fields and a strongly decreased average number of curved trichoid sensilla on distal segments. The great differences found in the studied family probably indicate a rapid function-related evolution of the antennal sensory surface structures in the caddisfly family Hydropsychidae.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47780902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemocyte proteome of the Lake Baikal endemic Eulimnogammarus verrucosus (Crustacea: Amphipoda) sheds light on immune-related proteins","authors":"E. Zolotovskaya, A. Nazarova, A. Saranchina, A. Mutin, P. Drozdova, Y. Lubyaga, M. Timofeyev","doi":"10.21638/spbu03.2021.402","DOIUrl":"https://doi.org/10.21638/spbu03.2021.402","url":null,"abstract":"Hemocytes are cells circulating in the hemolymph and playing an important role in crustacean immunity. These cells not only function as phagocytes but also express immune compounds to the hemolymph. Here we obtained hemocyte proteome of the endemic amphipod (Amphipoda, Crustacea) Eulimnogammarus verrucosus from Lake Baikal, the first hemocyte proteome of an amphipod, using liquid chromatography/tandem mass spectrometry (LC-MS/MS). A total of 1152 unique proteins were discovered with LC-MS/MS. We discovered both proteins directly involved in the immune response, such as pattern recognition proteins (C-type lectins), and compounds with antimicrobial activity (ctenidin and anti-lipopolysaccharide factor/scygonadin). Moreover, hemocyanins which may act as a phenoloxidase and C-type lectins were among the most diverse protein groups in the hemocyte proteome. The obtained data can be useful for further studies of immune components and mechanisms in Baikal amphipods.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43636269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agaricoid and boletoid fungi of Russia: the modern country-scale checklist of scientific names based on literature data","authors":"S. Bolshakov, L. Kalinina, E. Palomozhnykh, K. Potapov, D. Ageyev, S. Arslanov, N. Filippova, M. Palamarchuk, D. Tomchin, E. Voronina","doi":"10.21638/spbu03.2021.404","DOIUrl":"https://doi.org/10.21638/spbu03.2021.404","url":null,"abstract":"The authors of this paper summarize the majority of published data on the distribution of agaricoid and boletoid fungi recorded in Russia, covering the period from 1824 through 2020. A comprehensive list of 6867 scientific names based on 954 publications was compiled for the first time for the whole territory of Russia. All records have been checked through Index Fungorum. The work consists of a review section and five appendices. The review section discusses the intensity of field research and accumulation of data on the distribution of agaricoid and boletoid fungi in Russia, both historically and in its current state. The authors discuss the current state of knowledge on the biodiversity of regions of Russia and point out blank spots, thus providing a reference and an “action plan” for the future. Appendix A presents a list of 6142 taxa unambiguously ascribed to 3246 accepted current names. Appendix B contains 727 names that cannot be ascribed to any accepted current names unequivocally, with reasons given (e.g., no current name, wrong authors’ citations, absence from Index Fungorum). Names from both checklists are supplemented with data on the distribution of these taxa within the Russian Federation and references to published records. Appendix C contains a list of accepted current names reported from only one region. Appendix D is an overview of the nearly 200 years of research of agaricoid and boletoid fungi for all regions of Russia. Appendix E is a list of references used for checklists and study history preparation.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42309931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen use by plants and nitrogen flows after application of standard and biomodified nitrogen fertilizers on barley","authors":"A. Zavalin, V. Chebotar, A. Alferov, Lyudmila Chernova, E. Shcherbakova, E. Chizhevskaya","doi":"10.21638/spbu03.2021.401","DOIUrl":"https://doi.org/10.21638/spbu03.2021.401","url":null,"abstract":"The aim of our study was to assess the efficiency of application of biomodified nitrogen fertilizers for barley, to reveal the sources of nitrogen used for biomass formation with the use of the 15N stable isotope, and to study nitrogen flows in the system of fertilizers–soil–plants–atmosphere. We demonstrated in a model experiment the ability of the plant growth-promoting bacteria Bacillus subtilis Ch-13 to move from the granules of mineral fertilizers to plant roots and to colonize them effectively. The effectiveness of biomodified nitrogen fertilizers for barley, Nur variety, was assessed in a microfield trial. After the application of biomodified nitrogen fertilizers, the accumulation of 15N in the plants increased by 2–5 %, its incorporation in the soil decreased and gaseous losses were decreased by 7 % as compared with the use of the usual forms of fertilizers. The application of biomodified nitrogen fertilizers can be used in agricultural practice as a novel technology to regulate nitrogen flows in the system of fertilizers–soil–plants–atmosphere.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44197124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metaproteomic Comparison of Cryoconite Communities from Caucasian and Novaya Zemlya Glaciers","authors":"B. Zainullina, I. Babkina, A. Lobov, R. Tembotov, E. Abakumov","doi":"10.21203/rs.3.rs-999957/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-999957/v1","url":null,"abstract":"\u0000 Anthropogenic pollution strongly affects glacial microbiological communities and promotes glacial melting. In the early stages of glacial melting formation of small cylindrical holes (cryoconite) occurs. While the microbiome of cryoconite is well described, the effect of anthropogenic pollution on cryoconite microbiological communities still has not been fully understood. Thus, we performed an unbiased functional comparison of the cryoconite communities from the highly polluted Caucasian glaciers and from less polluted glaciers in Novaya Zemlya. For this purpose, we used the shotgun metaproteomics approach which has not been used for cryoconite microbiome analysis previously. We identified 475 protein groups, a third of which were found in both glaciers. Cryoconites in both glaciers have similar microbiological communities with Cyanobacteria as dominant phyla. Nevertheless, we found a slight shift from the dominance of phototrophic Cyanobacteria in Novaya Zemlya to heterotrophic bacteria in the Caucasus. We assume that it might be caused by anthropogenic pollution, but other factors such as differences in seasonal dynamics of microbiological communities should be tested in the future.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46731032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms","authors":"V. Zhukov, E. Zorin, A. Zhernakov, A. Afonin, G. Akhtemova, Andrej Bovin, A. Dolgikh, A. Gorshkov, E. Gribchenko, K. Ivanova, A. Kirienko, A. Kitaeva, M. Kliukova, O. Kulaeva, P. Kusakin, I. Leppyanen, O. Pavlova, D. Romanyuk, E. Rudaya, T. Serova, O. Shtark, A. Sulima, A. Tsyganova, E. Vasileva, E. Dolgikh, V. Tsyganov, I. Tikhonovich","doi":"10.21638/spbu03.2021.301","DOIUrl":"https://doi.org/10.21638/spbu03.2021.301","url":null,"abstract":"The garden pea (Pisum sativum L.), like most members of Fabaceae family, is capable of forming symbioses with beneficial soil microorganisms such as nodule bacteria (rhizobia), arbuscular mycorrhizal (AM) fungi and plant growth promoting bacteria (PGPB). The autoregulation of nodulation (AON) system is known to play an important role in controlling both the number of nodules and the level of root colonization by AM via root-to-shoot signaling mediated by CLAVATA/ESR-related (CLE) peptides and their receptors. In the pea, mutations in genes Sym28 (CLV2-like) and Sym29 (CLV1-like), which encode receptors for CLE peptides, lead to the supernodulation phenotype, i.e., excessive nodule formation. The aim of the present study was to analyze the response of pea cv. ‘Frisson’ (wild type) and mutants P64 (sym28) and P88 (sym29) to complex inoculation with rhizobia, AM fungi and PGPB, with regard to biomass accumulation, yield and transcriptomic alterations. The plants were grown in quartz sand for 2 and 4 weeks after inoculation with either rhizobia (Rh) or complex inoculation with Rh + AM, Rh + PGPB, and Rh+AM+PGPB, and the biomass and yield were assessed. Transcriptome sequencing of whole shoots and roots was performed using a modified RNAseq protocol named MACE (Massive Analysis of cDNA Ends). In the experimental conditions, P88 (sym29) plants demonstrated the best biomass accumulation and yield, as compared to the wild type and P64 (sym28) plants, whereas P64 (sym28) had the lowest rate of biomass and seed yield. The transcriptome analysis showed that both supernodulating mutants more actively responded to biotic and abiotic factors than the wild-type plants and demonstrated increased expression of genes characteristic to late stages of nodule development. The roots of P64 (sym28) plants responded to AM+Rh treatment with upregulation of genes encoding plastid proteins, which can be connected with the activation of carotenoid biosynthesis (namely, the non-mevalonate pathway that takes place in root plastids). The more active response to symbionts in P88 (sym29) plants, as compared to cv. ‘Frisson’, was associated with counterregulation of transcripts involved in chloroplast functioning and development in leaves, which accompanies successful plant development in symbiotic conditions. Finally, the effect of retardation of plant aging upon mycorrhization on a transcriptomic level was recorded for cv. ‘Frisson’ but not for P64 (sym28) and P88 (sym29) mutants, which points towards its possible connection with the AON system. The results of this work link the plant’s autoregulation with the responsiveness to inoculation with beneficial soil microorganisms.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41399081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of Notahypsibius pallidoides (Tardigrada: Hypsibiidae) in the fauna of Russia confirmed with the methods of DNA barcoding","authors":"Denis Tumanov","doi":"10.21638/spbu03.2021.309","DOIUrl":"https://doi.org/10.21638/spbu03.2021.309","url":null,"abstract":"A brief report is given on the tardigrade species Notahypsibius pallidoides, discovered in a moss cushion in the vicinity of Saint Petersburg. Morphological identification of the species is supported with the analysis of obtained sequences of nuclear and mitochondrial DNA barcode genes (ITS-2 and COI). The current state of knowledge of Russian fauna of Tardigrada is characterised, and all available data on the distribution of Notahypsibius pallidoides and its genetic variability are summarised. The data obtained in this study by methods of DNA barcoding demonstrated that studied specimens of the tardigrada population isolated in Russia belong to the same species (Notahypsibius pallidoides) as the control specimens isolated in Austria though represent another haplotype thus confirming the presence of Notahypsibius pallidoides for the fauna of Russia.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44046457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the polydispersity of soil-like bodies in glacier environments by the laser light scattering (diffraction) method","authors":"E. Abakumov, T. Nizamutdinov, V. Polyakov","doi":"10.21638/spbu03.2021.302","DOIUrl":"https://doi.org/10.21638/spbu03.2021.302","url":null,"abstract":"This study presents the results of polydispersity analysis of soil-like bodies from two various polar regions using the laser light scattering method. The differences in the particle size distribution of cryoconite samples from the Anuchin Glacier (Antarctica) and the Mushketov Glacier (Arctic) are described. The samples obtained from the Mushketov Glacier are characterized by a finer particle size distribution than samples collected on the Anuchin Glacier. While comparing our results with previously published studies, it was found that the method of laser light scattering shows a lower content of small fractions (<0.05 mm) compared to the classical methods of sedimentation, since these methods are based on fundamentally different physical principles. The laser method used requires low amounts of samples (0.2–0.5 g), while the classical sedimentary method uses a higher gravimetric portion of cryoconite (5–10 g), which is critical for field sampling.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45732629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual dimorphism patterns of the White Sea threespine stickleback (Gasterosteus aculeatus)","authors":"A. Dorgham, U. Candolin, T. Ivanova, M. Ivanov, E. Nadtochii, A. Yurtseva, D. Lajus","doi":"10.21638/spbu03.2021.307","DOIUrl":"https://doi.org/10.21638/spbu03.2021.307","url":null,"abstract":"Sexual dimorphism (SD) in the threespine stickleback Gasterosteus aculeatus reflects the different roles of the sexes in reproduction and their adaptations to different ecological niches. We quantified SD in one population of marine stickleback from the White Sea, collected during the spawning period from three spawning grounds, each at a distance of 5 km or less from the others. We used a landmark-based approach to quantify variation in 44 morphometric linear traits. In total, 749 females and 693 males were analyzed. In males, anterior body parts are larger — the base of the caudal fin and armor structures such as the first and second dorsal spines and the pelvic spine. Females have larger posterior bodies — the abdomen, pelvic girdle and the third dorsal spine. The SD of caudal body parts exhibits complex patterns. In White Sea threespine stickleback, SD patterns are generally similar to other populations of the species, but more often show male-biased patterns. Female-biased size SD may be associated with the female biased sex ratio of White Sea stickleback.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47341291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild-type p53-induced phosphatase sensitizes acute myeloid leukemia cells to conventional chemotherapy","authors":"V. Golotin, E. Belotserkovskaya, L. Girshova, A. Petukhov, A. Zaritsky, O. Demidov","doi":"10.21638/spbu03.2021.308","DOIUrl":"https://doi.org/10.21638/spbu03.2021.308","url":null,"abstract":"Recently wild-type p53-induced phosphatase was implicated in the pathogenesis of acute myeloid leukemia (AML) and “pre-leukemia” myeloproliferative conditions. Here we decided to check how the strategy directed to phosphatase inhibition affected sensitivity to conventional chemotherapy. All experiments were conducted on AML cell lines cultivated in vitro. The levels of wild-type p53-induced phosphatase vary in different AML cell lines. The chemical compound GSK2830371 reduced levels of phosphatase and diminished its activity. GSK2830371 did not significantly change the cell cycle distribution of AML cells when used alone or in combination with the anti-cancer chemotherapeutic drug Cytosar but increased caspase-dependent PARP1 cleavage. In contrast with previous studies, we did not observe the negative effect of phosphatase activity inhibition and depletion on cells when a chemical inhibitor was used as monotherapy. Using a combination of GSK2830371 with Cytosar we were able to reduce the threshold of chemotherapy-dependent cytotoxicity and more efficiently eliminate leukemic cells. We propose considering inhibition of wild-type p53-induced phosphatase as a prospective strategy in improving anti-AML therapy.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44553443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}