Journal of bioprocessing & biotechniques最新文献_第2页

Tissues are Shaped by Cells that Cover the Organ Surfaces: The Aviation Routes 组织是由覆盖在器官表面的细胞形成的:航空路线

Journal of bioprocessing & biotechniques Pub Date : 2021-01-01 DOI: 10.37421/2155-9821.2021.11.485

H. Kiran

引用次数: 0

The Anaerobic Reactor was Stacked with Semi-Delicate Media 厌氧反应器采用半精细介质堆砌

Journal of bioprocessing & biotechniques Pub Date : 2021-01-01 DOI: 10.37421/2155-9821.2021.11.486

J. Koshikh

引用次数: 0

Mammalian cells genetic manipulations for enhanced monoclonal antibody production: from traditional to novel trends, a review 用于增强单克隆抗体生产的哺乳动物细胞遗传操作:从传统到新的趋势，综述

Journal of bioprocessing & biotechniques Pub Date : 2020-01-01 DOI: 10.37421/JPBPT.2020.10.347

Pierre-Mehdi Hadbi

{"title":"Mammalian cells genetic manipulations for enhanced monoclonal antibody production: from traditional to novel trends, a review","authors":"Pierre-Mehdi Hadbi","doi":"10.37421/JPBPT.2020.10.347","DOIUrl":"https://doi.org/10.37421/JPBPT.2020.10.347","url":null,"abstract":"Novel therapeutics and monoclonal antibodies (mAbs) specifically, are under massive demands for the treatment of various illnesses. Research in cell engineering has been able to develop mammalian cell lines able to produce higher yields of potent recombinant proteins using different genetic manipulations. However, the public demands for these potent drugs keep increasing and as such, it is needed for the field of biopharmaceuticals to create highly efficient producers to increase volumetric productivity and lower overall costs. Several ways have been investigated up to date, from impacting on cells' natural growth pattern, by apoptosis regulation and cell cycle control, enhancement of its production pathways, by stimulating secretary factors, and production of enzymes to use growth-related metabolites. These approaches, most of the time, involve a considerable amount of resources and time, with an impact mostly on a couple of natural traits of mammalian cells. The ideal scenario is to use other vectors to impact entire pathways and the global cellular functioning to provide better workhorses. Novel approaches, namely micro RNAs (miRNAs), RNA interferences (RNAi) and CRISPR/Cas9, have been used in the last few years and have demonstrated a broader impact on cellular pathways, and also more efficiency in recombinant genes transfection or complete knock-out of specific sequences. In this review, traditional and novel aspects are described, discussed while expressing what approach to be taken next would be of interest, from a productivity standpoint.","PeriodicalId":89963,"journal":{"name":"Journal of bioprocessing & biotechniques","volume":"10 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70061821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioproducts from Miscanthus 芒草的生物制品

Journal of bioprocessing & biotechniques Pub Date : 2018-10-17 DOI: 10.4172/2155-9821-C1-014

pMarina G Kalyuzhnayap

引用次数: 0

Effects of biologically active substances on the rabbit fertility 生物活性物质对家兔生育能力的影响

Journal of bioprocessing & biotechniques Pub Date : 2018-10-17 DOI: 10.4172/2155-9821-C1-016

P. Párkányi, Jan Rafayp

{"title":"Effects of biologically active substances on the rabbit fertility","authors":"P. Párkányi, Jan Rafayp","doi":"10.4172/2155-9821-C1-016","DOIUrl":"https://doi.org/10.4172/2155-9821-C1-016","url":null,"abstract":"Microbial Fuel Cell (MFC) is a device in which microorganisms consume organic compounds as nutrient source and discharge electrons to the electrode, thereby generating electricity. In this study, double chamber MFCs and multiple chambers MFCs were constructed for the generation of electricity from microorganisms present in organic waste samples. Samples were collected from organic wastes from local garbage dumping area in wetland and electricity was generated by the oxidation of endogenous microbes present in samples. Electricity production was gradually increased with growth of organisms, which was decreased after time interval due to depletion of organic matter. A steady state for electricity generation was maintained by adding external glucose. In total, 44 bacteria were isolated from the anodic biofilm. The electrogenic activity of each isolate was observed using artificial wastewater (without organic matter) as substrate. A significant generation of electricity (Maximum 5.78 V and 5.03 mA in multiple chambers MFC) was attained connecting multiple chambers containing MFCs and able to lid light. Microbial diversity on anodic biofilm was observed by scanning electron microscope (SEM) image analysis. Characterization of anodic biofilm bacterial community suggested that 54.54% of electrogenic bacterial community belonged to Enterobacteriaceae family. In addition, the non-fermentative genera Pseudomonas, Moraxella, Vibrio, Burkholderia, Escherichia, Enterobacter, Photobacterium, Obesumbacterium, Sphingomonas and Raoultella also played an important role. MFC, a renewable method for electricity generation from biodegradable compounds without emission of carbon dioxide, is crucial for sustainable in electricity production in countries like Bangladesh as an environment friendly approach.","PeriodicalId":89963,"journal":{"name":"Journal of bioprocessing & biotechniques","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70322056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Depolimerization of marine alginates improves their health benefits in human 海藻酸盐的解聚作用提高了其对人体的健康益处

Journal of bioprocessing & biotechniques Pub Date : 2018-10-17 DOI: 10.4172/2155-9821-C1-015

pMaksim Khotimchenko Elena Khozhaenko Valeri Kovalev Rodio Khootimchenko, Maria Filp

引用次数: 0

Effect of Pre-Drying and Hydrocolloids Batter Coating during Heat and Moisture Transfer of Coated Fried Potato Chips 预干燥和水胶体面糊包衣对油炸薯片湿热传递的影响

Journal of bioprocessing & biotechniques Pub Date : 2018-05-31 DOI: 10.4172/2155-9821.1000328

S. S. Sobowale, B. O. Omotoso

{"title":"Effect of Pre-Drying and Hydrocolloids Batter Coating during Heat and Moisture Transfer of Coated Fried Potato Chips","authors":"S. S. Sobowale, B. O. Omotoso","doi":"10.4172/2155-9821.1000328","DOIUrl":"https://doi.org/10.4172/2155-9821.1000328","url":null,"abstract":"Fat uptake is considered the major nutritional critical point of deep frying because of the obesity and negative effect of excess oil consumption on human health. Hence, this study investigated the effect of pre-drying and hydrocolloids batter coating during heat and moisture transfer of coated fried potato chips. The potato slices were pre-dried coated and deep fried at 180°C for 5 min. Chemical composition, color profile; thermo-physical properties, dimensionless numbers associated with convective heat and mass transfer coefficient and sensory properties were evaluated. The result revealed that chemical composition and color profile were significantly (p ≤ 0.05) affected by pre-drying and hydrocolloids batter coating. The density, viscosity, thermal conductivity, specific heat capacity and thermal diffusivity ranged from 333.3-444.0 kg/m3, 0.5281-0.5377 pas, 22.686-31.403 W/m °C, 198.686-258.383 KJ/kgK and 2.730 × 10-4-3.826 × 10-4 m2/s, respectively. The estimated Grashof, Prandtl, Schmidt, Nusselt and Sherwood numbers ranged between 0.024-0.032, 4.14-4.71, 2.053 × 10-5 and 2.739 × 10-5, 0.0167-0.0209, 9.70 × 10-8 and 1.02 × 10-7, respectively. The heat and mass transfer coefficient values ranged from 25.26 to 42.98 W/m2 °C and 3.80 × 10-12 to 4.01 × 10-12 m/s, respectively. Hydrocolloid type distinctly dictated considerable quality attributes and apparently potato chips pre-dried, coated with egg albumin and carboxyl methylcellulose were gum tragacanth demonstrates great reduction in the fat content of the chips which will in turn decline the tendency of obesity and equally reduce cholesterol level.","PeriodicalId":89963,"journal":{"name":"Journal of bioprocessing & biotechniques","volume":"8 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2018-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-9821.1000328","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43367258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Influence of Technical and Microbiological Parameters on the Hygienic Quality of Coffee from Fully Automated Coffee Machines 全自动咖啡机工艺及微生物参数对咖啡卫生品质的影响

Journal of bioprocessing & biotechniques Pub Date : 2018-04-28 DOI: 10.4172/2155-9821.1000322

Jan Schages, B. Brands, P. Heldt, R. Kohlmann, Andre Pickel, Bockmhl Dp

{"title":"The Influence of Technical and Microbiological Parameters on the Hygienic Quality of Coffee from Fully Automated Coffee Machines","authors":"Jan Schages, B. Brands, P. Heldt, R. Kohlmann, Andre Pickel, Bockmhl Dp","doi":"10.4172/2155-9821.1000322","DOIUrl":"https://doi.org/10.4172/2155-9821.1000322","url":null,"abstract":"A rising number of people drink their coffee on their way to work (or “to go”), causing an increased usage of single-use mugs. Efforts to lower this number by using multi-use mugs are often not supported by coffee shops due to doubts about food safety. The data collected in this study, show that the number detected in multi-use mugs can be neglected compared to the number of microorganisms found in freshly prepared coffee. The bacteria found were inter alia part of the human skin flora (e.g., Staphylococcus epidermidis), ubiquitous bacteria as Acinetobacter johnsonii, water bacteria like Pseudomonas aeruginosa and food spoiling bacteria as Bacillus cereus as well as different Enterobacteriaceae. \u0000Furthermore, our data suggest that a coffee output temperature of 68°C or higher significantly reduces the number of microorganisms present in the brewed beverage. These findings do not only help to enhance food safety, but can at the same time reduce the number of single-use mugs as the microbiological risk associated with multi-use mugs seems to be overestimated.","PeriodicalId":89963,"journal":{"name":"Journal of bioprocessing & biotechniques","volume":"8 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-9821.1000322","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44953784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Case of Advanced Pulmonary Adenocarcinoma Harboring HER-2 Amplification Treated with Afatinib 阿法替尼治疗晚期肺腺癌伴HER-2扩增1例

Journal of bioprocessing & biotechniques Pub Date : 2018-04-23 DOI: 10.4172/2155-9821.1000321

Yan Lu, Jiahui Lan, Ling Bi, Lanping Xu

引用次数: 0

Detection and Enumeration of Coliforms in Ganga Water Collected from Different Ghats 不同高止山脉恒河水中大肠杆菌的检测与计数

Journal of bioprocessing & biotechniques Pub Date : 2018-03-31 DOI: 10.4172/2155-9821.1000320

Nitika Matta, Bisht Grs

引用次数: 5