ISBT science series最新文献

{"title":"Red cell genotyping in Thailand: trends for blood transfusion in Asian populations","authors":"Oytip Nathalang","doi":"10.1111/voxs.12547","DOIUrl":"10.1111/voxs.12547","url":null,"abstract":"<p>Numerous molecular techniques have been employed to determine well-matched blood units for patients in need, particularly those requiring blood transfusions within 3 months and presenting positive DAT results. Only specialized reference laboratories use commercially available kits for red cell (RC) genotyping due to their expense and requiring extremely sophisticated instruments. Moreover, regarding complicated cases, particular typing reagents may be unavailable or insufficient. Therefore, identifying and confirming the compatibility of the blood units donated would require more time. This article aims to review the present uses of RC genotyping in Thailand and their significant contributions to transfusion practices in Asian populations. RC genotyping using PCR-sequence-specific primer (PCR-SSP) and multiplex PCR (M-PCR) has been established and used in patient and donor populations. The results of genotyping acquired by PCR-SSP and M-PCR were precise and in agreement with serological techniques. Predicted phenotypes of clinically significant blood group systems have been used to determine the compatibility of RC units among Thai patients having multiple antibodies. As a result, one additional advantage is the expanded storing of donated, frozen rare blood types. RC genotyping for high- and low-prevalence antigens among differing Asian ethnicities requires consideration. RC genotyping employing these advanced PCR techniques could provide testing results that are both reliable and appropriate to screen suitable donor blood units in complex cases or patients presenting rare blood types. In addition, comparable strategies could be employed in other Asian populations using limited resources.</p>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 3","pages":"310-314"},"PeriodicalIF":0.0,"publicationDate":"2020-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12547","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48727380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfusion in Sickle cell disease: best practice and understanding of its utility","authors":"Patricia A. Shi,&nbsp;Karina Yazdanbakhsh","doi":"10.1111/voxs.12549","DOIUrl":"10.1111/voxs.12549","url":null,"abstract":"<p>Transfusion therapy remains an important treatment modality for patients with sickle cell disease (SCD). Transfusions are given not only to treat symptomatic anaemia, but also to decrease SCD-related complications by lowering the percentage of circulating sickle red cells, thus decreasing blood viscosity and vaso-occlusion. The strongest evidence of the effectiveness of transfusion is in primary and secondary stroke prophylaxis, where transfusion reduces the risk of overt stroke by about 90–70%, and in pre-operative transfusion, where transfusion reduces the risk of acute chest syndrome by at least 30%. Most other indications for transfusion in SCD are based on expert opinion or less well-controlled studies. In this brief overview, we discuss methods of transfusion, the evidence and expert opinion regarding various indications in sickle cell disease and our current pathophysiologic understanding of how transfusions may work in SCD.</p>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 3","pages":"347-351"},"PeriodicalIF":0.0,"publicationDate":"2020-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47250361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening for the human neutrophil antigen-2 deficiency by a multiplex one-tube polymerase chain reaction assay","authors":"Jiwu Gong,&nbsp;Meiying Rao,&nbsp;Zhonghua Jia,&nbsp;Na Ma,&nbsp;Lifang Sun,&nbsp;Xiaonan Yu,&nbsp;Feng Chen,&nbsp;Jue Xie,&nbsp;Hongwei Gong,&nbsp;Qiang Fu,&nbsp;Chen Cao,&nbsp;Fangfang Chen,&nbsp;Tongmao Zhao","doi":"10.1111/voxs.12546","DOIUrl":"10.1111/voxs.12546","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this study was to develop a rapid method for human neutrophil antigen-2 (HNA-2) genotyping and use it for fast screening for the HNA-2 deficiency phenotype in the Chinese population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>HNA-2 is encoded by the <i>CD177</i> gene, and a single nucleotide polymorphism (SNP) at cDNA position 787 (<i>CD177</i>*787 A &gt; T) creates a stop codon, which is responsible for the HNA-2 deficiency phenotype. Recently, the SNP c.787A &gt; T has been demonstrated to be the primary genetic mechanism for HNA-2 deficiency.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study focused on determination of CD177 SNP c.787A &gt; T. We designed a set of allele- and sequence-specific primers (SSP), which were used to develop a one-tube polymerase chain reaction (PCR) assay for HNA-2 genotyping. The HNA-2 genotype of a total of 2185 Chinese blood donors was determined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The validation of this HNA-2 genotyping assay was confirmed by sequencing analysis. The HNA-2 two alleles, <i>CD177*</i>787A and 787T, can be simultaneously determined in a single tube PCR. Thirteen individuals with the <i>CD177*</i>787AT genotype were detected in this study. The <i>CD177*</i>787TT homozygous genotype was absent, suggesting that HNA-2 deficiency is rare in the Chinese population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The validated assay described in this study is a rapid and reliable method for HNA-2 genotyping, which may be used for fast screening for HNA-2 deficiency.</p>\u0000 </section>\u0000 </div>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 2","pages":"286-291"},"PeriodicalIF":0.0,"publicationDate":"2020-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12546","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42598450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kodecytes: modifying the surface of red blood cells","authors":"Stephen Henry","doi":"10.1111/voxs.12545","DOIUrl":"10.1111/voxs.12545","url":null,"abstract":"<p>There are few routine techniques available to modify the antigenic surface of red blood cells (RBC). Primarily, modification techniques involve antigen destruction with enzymes or denaturing chemicals. In contrast, very few antigen additive technologies exist. One antigen additive technology involves the passive insertion of Function-Spacer-Lipid constructs (Kode Technology) into the cell membrane. Kode constructs can label any cell (creating kodecytes) with controlled levels of almost any small molecule, including blood group carbohydrate and peptide antigens. They have been used to modify RBCs with a variety of ABH, Lewis and related antigens to study the specificity of monoclonal and polyclonal antibodies. Although kodecytes with carbohydrate epitopes have been extensively evaluated, those with peptide antigens also exist and some can be prepared with cross-reactive bacterially related peptide antigens, to create IgG-reactive kodecytes. These so-called ASKGkodecytes can mimic all serological IgG reactions and are particularly suited for teaching and training. Recently kodecytes have also been used to quantify ABH antibody levels without the requirement for plasma dilution, and in this review the probable mechanism of action is explained. In general, kodecytes allow for the controlled antigen modification of RBCs and have the ability to make almost unlimited quantities of antigen rare or unusual RBCs from common resources. Not only are kodecytes useful research tools to study RBCs <i>in vitro</i> and <i>in vivo</i> but they also have the potential to create standardized diagnostics, quality control systems, advanced serological teaching panels and novel research tools.</p>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 3","pages":"303-309"},"PeriodicalIF":0.0,"publicationDate":"2020-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48302403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An insight into Indonesian current thalassaemia care and challenges","authors":"Pustika Amalia Wahidiyat,&nbsp;Teny Tjitra Sari,&nbsp;Ludi Dhyani Rahmartani,&nbsp;Iswari Setianingsih,&nbsp;Stephen Diah Iskandar,&nbsp;Anastasia Michelle Pratanata,&nbsp;Ivana Yapiy,&nbsp;Mikhael Yosia,&nbsp;Fernando Tricta","doi":"10.1111/voxs.12544","DOIUrl":"10.1111/voxs.12544","url":null,"abstract":"<p>Thalassaemia is one of the most prevalent inherited blood disorders in Indonesia, with highly diverse mutations ranging from mild to severe that can be found across the nation. Nevertheless, thalassaemia management in Indonesia is still limited to supportive treatment, such as blood transfusion, iron chelation, complications monitoring, psychosocial support and a comfortable transition from child to adult clinic. However, these managements are still suboptimal in most parts of the nation. Indonesia still has a long way from implementing the optimal curative treatment for thalassaemia. Iron chelators are sometimes not available, especially in rural areas. The cost for optimal dosages of iron chelation also cannot be fully covered by the current national health insurance scheme. However, it still benefits our patients, considering it is the only treatment to decrease iron deposition in organs. With that situation, our patients, both paediatric and adults, have normal cardiac haemosiderosis, moderate-to-severe hepatic haemosiderosis and normal to mild pancreatic haemosiderosis. Therefore, the number of deaths, especially those due to heart failure and infection, was significantly reduced. An improvement in thalassaemia supportive treatments is in line with the increase in patients’ life expectancy. Without curative treatment options, the lifelong cost for treatment will extremely burden the national health budget. To date, thalassaemia stands for the 5th most costly disease in Indonesia. Therefore, a screening programme must be realized soon; hence, the treatment cost can be allocated for initiating the transplant unit or improving other important areas.</p>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 3","pages":"334-341"},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43185726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jehovah's Witnesses and transfusion: where do we stand in Europe?","authors":"Sébastien Loix,&nbsp;Pierre Henin,&nbsp;Olivier Descamps,&nbsp;Isabelle Reusens","doi":"10.1111/voxs.12542","DOIUrl":"10.1111/voxs.12542","url":null,"abstract":"<p>Transfusion refusal expressed by Jehovah's Witnesses has always been questioning for medical community. However, abundant literature exists treating about how to manage difficult medical bleeding situations in Jehovah's witnesses, there is a few information available on the legal and ethical aspects of transfusion refusal. Since 1994, Europe has putted a spotlight on patient's right. The question of patient's autonomy and consent have taken a predominant place in care. In order to summarize the actual situation in Europe, this work intends to draw a global view on legal, ethical and medical aspects of transfusion refusal expressed by Jehovah witnesses.</p>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 2","pages":"212-220"},"PeriodicalIF":0.0,"publicationDate":"2019-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12542","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46018708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between the innate immune system and immune haemolytic anaemia","authors":"Laura Delvasto-Nuñez,&nbsp;Ilse Jongerius,&nbsp;Sacha Zeerleder","doi":"10.1111/voxs.12541","DOIUrl":"10.1111/voxs.12541","url":null,"abstract":"<p>Immune haemolytic anaemia (IHA) is characterized by an increased breakdown of red blood cells (RBCs) due to allo- or auto-antibodies directed to RBC antigens with or without complement activation. Based on the nature of the antibodies, IHA can be divided in three main categories: autoimmune, drug-induced and alloimmune-mediated IHA. There is growing evidence that the innate immune system plays an important role in the pathogenesis of IHA. Complement-mediated haemolysis with the subsequent release of cell-free haemoglobin and cell-free haem resulting in the generation of reactive oxygen species and cytotoxicity as well as the production of anaphylatoxins induce a systemic inflammatory response, which contributes to morbidity and mortality in IHA. The natural plasma scavengers of cell-free haemoglobin and cell-free haem, haptoglobin and hemopexin, respectively, are depleted in cases of chronic or severe IHA. The inducible enzyme haem oxygenase 1 (HO-1) is an efficient cellular scavenger degrading haem into anti-inflammatory products to partially limit haem-mediated oxidative damage in cases of saturated scavenging capacity. Complement-targeted therapy and the therapeutic replenishment of haptoglobin and hemopexin as well as the induction of HO-1 expression might be suitable targets in the treatment of IHA.</p>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 1","pages":"91-101"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12541","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44673952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of salt supplementation on vasovagal reactions in young whole blood donors","authors":"Karan Kumar,&nbsp;Suchet Sachdev,&nbsp;Neelam Marwaha,&nbsp;Ratti Ram Sharma","doi":"10.1111/voxs.12538","DOIUrl":"10.1111/voxs.12538","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The physiologic interventions to prevent vasovagal reactions (VVRs) have been published evaluating water loading, applied muscle tension and salt loading on immediate VVRs (IVVRs), but the effect of salt on delayed VVRs (DVVRs) has not been evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Randomized controlled study was conducted enrolling 3060 donors in age group 18–25 years. Control group (CG) donors were given 300 ml of sweetened lime water, with additional 2·5 g salt added in the test group (TG).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There were 90 VVRs in 3060 (2·94%, 1 in 34) young college-going whole blood donors in the present study. This included 57 IVVRs (1·86%, 1 in 54) and 33 DVVRs (1·08%, 1 in 93). There were 53 VVRs (3·49%, 1 in 29) in the CG and 37 VVRs (2·39%, 1 in 57) in the TG, with absolute risk reduction (ARR) of 1·1%. There were 30 IVVRs (1·98%, 1 in 51) in CG and 27 IVVRs (1·74%, 1 in 57) in TG, with ARR of 0·2%%. Significantly, there were 23 DVVRs (1·51%, 1 in 67) in CG and only 10 DVVRs (0·65%, 1 in 154) in the TG, with ARR of 0·9%. One thousand (1000), five hundred (500) and one hundred and eleven (111) young whole donors need to be given 2·5 g of salt supplementation in fluid medium to prevent one VVR, IVVR and DVVR, respectively. The odds and the adjusted odds (AOR) of occurrence of DVVRs were 2·36, 95% CI (1·12–4·98) and 2·40, 95% CI (1·13–5·07) in the CG as against in the TG, on binary and multivariable logistic regression, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>There is an overall trend of decrease in VVRs with salt loading in young college-going whole blood donors, which is statistically evident in the case of DVVRs.</p>\u0000 </section>\u0000 </div>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 2","pages":"253-260"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46149579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention strategies of transfusion-transmitted parasitic infections (TTPIs) in Iran, an endemic area for malaria, toxoplasmosis and leishmaniasis","authors":"Ahmad Mardani","doi":"10.1111/voxs.12534","DOIUrl":"10.1111/voxs.12534","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>Parasitic infections are one of the most important adverse reactions of blood transfusion. Several strategies are being implemented in blood transfusion centers of the world to prevent the transfusion-transmitted parasitic infections (TTPIs). The objective of this work was to clarify and describe the strategies to minimize the transmission risk of endemic parasitic agents in Iran via blood transfusion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This study was conducted in the Iranian blood transfusion organization (IBTO). The data were extracted from the latest version of the “medical interview” standard operating procedure (SOP) and then recorded in the prepared sheets.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The donor selection is the first and only step to reduce the risk of TTPIs in endemic and non-endemic areas of Iran. In all blood transfusion centers of the IBTO, the blood donation volunteers with a previous history of malaria, Chagas disease, visceral leishmaniasis (VL), muco-cutaneous leishmaniasis and babesiosis, as well as those with toxoplasmosis, cutaneous leishmaniasis (CL) and with a history of residence in, or travel to, malaria-endemic areas are permanently or temporarily deferred from the blood donation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>For some reasons, including the prevalence of malaria, toxoplasmosis and VL in parts of Iran, the donor selection strategy is not sufficient to prevent the TTPIs. Therefore, the changing of donor selection process and the use of other common preventive strategies are recommended to reduce the risk of TTPIs, especially for high-risk groups of toxoplasmosis and VL.</p>\u0000 </section>\u0000 </div>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"14 4","pages":"401-408"},"PeriodicalIF":0.0,"publicationDate":"2019-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12534","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43858516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comfortable environment for blood donation lowers the rate of vasovagal reactions","authors":"Tatsuma Hashizume,&nbsp;Fumihiko Ishimaru,&nbsp;Gaku Kondo,&nbsp;Noriko Namba,&nbsp;Yoshiko Miura,&nbsp;Seiko Aota,&nbsp;Yumiko Nishitani,&nbsp;Noriko Kunii,&nbsp;Hiromi Misawa,&nbsp;Reiko Shibata,&nbsp;Yoshihiro Sawamura,&nbsp;Koji Matsuzaki,&nbsp;Kazunori Nakajima,&nbsp;Tsuneo Kato","doi":"10.1111/voxs.12540","DOIUrl":"10.1111/voxs.12540","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>To evaluate the possible effects of environmental surroundings on blood donor reactions, we analysed vasovagal reaction rates before and after renovation of the Yurakucho Blood Donation Center in Tokyo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>We conducted a retrospective cohort study based on data stored in a computerized database. The vasovagal reaction rates associated with blood donation during two separate 3-year periods were compared, that is prior to (October 2007 to September 2010) and after (October 2010 to September 2013) the renovation of the Yurakucho Blood Donation Center. The renovation included the following interventions: (1) expansion of the phlebotomy area from 188·0 to 322·4 m² to accommodate 22 beds; (2) expansion of the reception/lounge area from 155·2 to 267·1 m² with modification for a more relaxed atmosphere; and 3) addition of well-controlled air conditioning.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The total reaction rate decreased from 0·65% (1302 reactions/200 181 donations) to 0·32% (606 reactions/191 966 donations) before and after the renovation, respectively, representing a 51% reduction (<i>P</i> &lt; 0·001). During the pre-intervention period, reaction rates in winter were higher than those in other seasons, and the reaction rate was correlated with room temperature. It was also suggested that environmental factors other than room temperature might play an important role. Multivariate logistic regression analysis of risk factors demonstrated that the male gender was the strongest favourable factor and young age was the next to strongest favourable factor.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our results suggest that providing a comfortable environment for blood donation may lead to fewer vasovagal reactions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":89948,"journal":{"name":"ISBT science series","volume":"15 2","pages":"261-265"},"PeriodicalIF":0.0,"publicationDate":"2019-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/voxs.12540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44522854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}