Advancements in genetic engineering最新文献_第8页

The challenge and opportunity for genetic engineering to provide unique solutions to complex biomedical practices: The rabies paradigm 基因工程为复杂生物医学实践提供独特解决方案的挑战和机遇:狂犬病范例

Advancements in genetic engineering Pub Date : 2013-10-30 DOI: 10.4172/2169-0111.S1.004

Charles E Rupprecht

引用次数: 26

Ideal Open Access (OA2) and the Lineage of Reproducibility 理想开放存取(OA2)和可再现性谱系

Advancements in genetic engineering Pub Date : 2013-10-18 DOI: 10.4172/2169-0111.1000E102

Yue Zhang

引用次数: 0

Pseudohypoparathyroidism Type Ia-Clinical Case with a Novel Mutation of GNAS1 Gene 假性甲状旁腺功能减退症伴GNAS1基因新突变1例

Advancements in genetic engineering Pub Date : 2013-10-11 DOI: 10.4172/2169-0111.1000115

Hanaa Zidan, S. Steinberg, A. Kansra

{"title":"Pseudohypoparathyroidism Type Ia-Clinical Case with a Novel Mutation of GNAS1 Gene","authors":"Hanaa Zidan, S. Steinberg, A. Kansra","doi":"10.4172/2169-0111.1000115","DOIUrl":"https://doi.org/10.4172/2169-0111.1000115","url":null,"abstract":"Pseudohypoparathyroidism (PHP) is a rare autosomal dominant disorder resulting from loss of function mutations in the GNAS gene. Several forms of PHP are noted. PHP type 1a occurs most commonly and is characterized by physical features termed Albright’s Hereditary Osteodystrophy (AHO), a constellation of physical features which may include short stature, obesity, round facies, heterotopic ossification, brachydactyly and mental retardation, and increased levels of parathyroid hormone (PTH) due to the end organ hormone resistance to its action. Here we report a new GNAS mutation in a 3.5 years old African American female patient with a history of round facies, developmental delays, obesity and seizure disorder; she was admitted for apneic episode and noted to have prolonged QTc interval on cardiac monitor. A lab evaluation showed severe hypocalcaemia, hyperphosphatemia, high PTH with normal magnesium and alkaline phosphatase levels. She also had slightly elevated Thyroid Stimulating Hormone (TSH) levels indicative of type 1 a PHP where resistance to multiple Gs protein-coupled hormones (e.g. PTH, TSH, Luteinizing Hormone (LH), Follicular Stimulating Hormone (FSH), and Growth Hormone Releasing Hormone (GHRH)) is present. Full genomic DNA sequencing of the exons and adjacent intronic regions of the GNAS gene revealed a novel heterozygous mutation in intron 7, c.585+1G>A, in both the patient and her mother.","PeriodicalId":89733,"journal":{"name":"Advancements in genetic engineering","volume":"2013 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2013-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2169-0111.1000115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70874913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cytochrome P450 1A1 and Glutathione S-Transferase Pi 1 Mutations in Pharyngeal and Laryngeal Carcinoma 细胞色素P450 1A1和谷胱甘肽s -转移酶Pi 1在咽喉癌中的突变

Advancements in genetic engineering Pub Date : 2013-09-27 DOI: 10.4172/2169-0111.1000113

N. Masood, M. Kayani

{"title":"Cytochrome P450 1A1 and Glutathione S-Transferase Pi 1 Mutations in Pharyngeal and Laryngeal Carcinoma","authors":"N. Masood, M. Kayani","doi":"10.4172/2169-0111.1000113","DOIUrl":"https://doi.org/10.4172/2169-0111.1000113","url":null,"abstract":"In the current case control study, 94 pharyngeal, 67 laryngeal cancer cases and 150 cancer free controls were screened via PCR-SSCP assay. Mean ages of pharyngeal, laryngeal cancer patients and control was 48.14 (± 16.7), 48.56 (± 17.4) and 46 (± 17.69) years respectively. Results revealed two novel mutations in CYP1A1 gene, a substitution mutation of A2842C resulting in missense tyrosine to serine formation and frameshift mutation due to insertion of thymidine at nucleotide 2842 resulting in 495 nucleotide sequences to alter. It was found that 3.2% pharyngeal and 2.98% laryngeal cancer patients had these mutations in CYP1A1. In GSTP1 gene exon 7, an A2848T substitution causes a leucine to leucine formation whereas G2849A substitution causes alanine to threonine formation at amino acid 166 and 167 respectively. These exonic mutations were found in 7.4% pharyngeal cancer and 9% laryngeal cancer patients. Two intronic deletions of C at nucleotide 1074 and 1466 were found in 1% pharyngeal and laryngeal cancer patients. Accumulation of mutations in CYP1A1 and GSTP1 genes seem to be associated with increased risk of pharyngeal and laryngeal cancer development.","PeriodicalId":89733,"journal":{"name":"Advancements in genetic engineering","volume":"55 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70874841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Status of Sugarcane Transgenic: an Overview 甘蔗转基因研究现状综述

Advancements in genetic engineering Pub Date : 2013-09-04 DOI: 10.4172/2169-0111.1000112

R. Singh, Pradeep Kumar, N. Tiwari, J. Rastogi, S. Singh

{"title":"Current Status of Sugarcane Transgenic: an Overview","authors":"R. Singh, Pradeep Kumar, N. Tiwari, J. Rastogi, S. Singh","doi":"10.4172/2169-0111.1000112","DOIUrl":"https://doi.org/10.4172/2169-0111.1000112","url":null,"abstract":"Sugarcane (Saccharum sps. Hybrids) is one of the most important industrial crops for sugar and biofuel production of the world. Substantial resources are being invested worldwide for sugarcane improvement through conventional and molecular breeding. The recombinant DNA technology has potential, via genetic engineering, to incorporate a specific gene which controls a particular trait, without co-transfer of undesirable genes from donor species as occurs in conventional breeding. A remarkable development is creation of sugarcane transgenic plants with improved yield characteristic and high value products. Now a days, transgenic traits has an integral part of maize, soya been, rice, tomato, cotton and oil seed breeding. Production of sugarcane transgenic events is routine in various laboratories of the world. In recent year, the potential of transformation technology in sugarcane improvement has been demonstrated with commercialization of commercial cultivars with multiple resistances. The globally cultivated area of transgenic crops has increased more than 81 million hectares. However, there are several technical limitations that still remain for multi gene transformation and creation of economically viable transgenic events. The demerits of many cultivars are not easily removed by transformation, and tools and technologies for precised integration and controlled expression of transgene have yet to be demonstrated in sugarcane. Present article exploring the possibility of sugarcane transgenic development through different methodologies and discuss the opportunities and challenges of developing and evolving commercial transgenic sugarcane based on the information available globally.","PeriodicalId":89733,"journal":{"name":"Advancements in genetic engineering","volume":"2 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70874786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Genetic engineering in Banana and Plantain 香蕉和大蕉的基因工程

Advancements in genetic engineering Pub Date : 2013-08-19 DOI: 10.4172/2169-0111.1000114

S. Elayabalan, Kalaiponmani Kalaimughilan

{"title":"Genetic engineering in Banana and Plantain","authors":"S. Elayabalan, Kalaiponmani Kalaimughilan","doi":"10.4172/2169-0111.1000114","DOIUrl":"https://doi.org/10.4172/2169-0111.1000114","url":null,"abstract":"Bananas are one among the world’s leading food crops, after rice, wheat and maize. Almost ninety percent of production is consumed in the production areas, especially in the poorest countries in Africa, Latin America and Asia. In certain regions, pureed banana is the first solid food given to infants. Bananas contribute to reducing food insecurity in producer country populations. Their composition, which includes high carbohydrates and minerals, makes them a staple calorie resource for over 500 million inhabitants of tropical countries. Considering the nutrition aspect, it is the world’s leading fruit crop, and in terms of economical value it is ranked as fifth economically important agricultural crop in world trade. In the global production of banana India contributes 29.19% as leading country. \u0000Bananas face numerous environmental challenges, particularly with fungal, bacterial as well as the major threatening disease like banana bunchy top virus. The problem is further aggravated by the limited diversity of banana cultivars around the world. Conventional breeding methods have limited success due to low female fertility, sterility, ploidy levels and poor seed set, besides the process is time consuming. These problems point to the necessity of developing alternate strategies for banana improvement through advancement of biotechnology tools like tissue culture and transgenic technology to improve the bananas. In this regard I will be discussing the current status of Banana improvement using biotechnology and future prospects.","PeriodicalId":89733,"journal":{"name":"Advancements in genetic engineering","volume":"2013 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2169-0111.1000114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70875025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Integrating Bioethical Concerns into Biosafety Law for Genetic Modification Technologies in Malaysia 将生物伦理问题纳入马来西亚转基因技术的生物安全法

Advancements in genetic engineering Pub Date : 2013-08-19 DOI: 10.4172/2169-0111.1000111

S. H. Idris

{"title":"Integrating Bioethical Concerns into Biosafety Law for Genetic Modification Technologies in Malaysia","authors":"S. H. Idris","doi":"10.4172/2169-0111.1000111","DOIUrl":"https://doi.org/10.4172/2169-0111.1000111","url":null,"abstract":"In considering the use of modern biotechnologies, specifically genetically modified organisms (GMOs), the Malaysian government recognizes the significant potential benefits as well as uncertainties, risks and doubts of this emerging technology. Though great benefits of this technology could help meet future needs, yet, this technology is often accompanied by public debate over its potential risks, which includes bioethical issues. In mitigating these risks in a sustainable manner, biosafety framework is required in order to protect human, plant and animal health, the environment and biodiversity. \u0000One of the means to manage the risks is through rule of justice based on law as a decisive technology solution. The government passed the Biosafety Act in 2007 to serve as an “umbrella act” which include the setting up of the National Biosafety Board as well as legal and institutional provisions tailored to comply with the Cartagena Protocol on Biosafety, with the objective to regulate the import, export, deliberate release, contained use and marketing of GMOrelated products in order to protect human, plant and animal health, the environment and biodiversity. The question is how does this law addressing bioethical issues and how effective the law in addressing this issue? The purpose of this paper is to analyse the extent to which this Biosafety Act 2007 and its regulations may be effectively integrating bioethical issues relating to GM crops in realizing its objectives. The article specifically focuses on bioethical issues provisions of GMOs under the Act and its regulations.","PeriodicalId":89733,"journal":{"name":"Advancements in genetic engineering","volume":"2 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70874709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The European Union Regulatory Framework on Genetically Modified Organisms and Derived Foods and Feeds 欧盟转基因生物及衍生食品和饲料监管框架

Advancements in genetic engineering Pub Date : 2013-08-19 DOI: 10.4172/2169-0111.1000109

M. Schauzu

引用次数: 9

Policy Analysis of Sustainable GMO Management Using Decision Making Method in Indonesia 基于决策方法的印尼转基因生物可持续管理政策分析

Advancements in genetic engineering Pub Date : 2013-06-24 DOI: 10.4172/2169-0111.1000107

P. Deswina, R. Syarief, Rachman Lm, M. Herman

{"title":"Policy Analysis of Sustainable GMO Management Using Decision Making Method in Indonesia","authors":"P. Deswina, R. Syarief, Rachman Lm, M. Herman","doi":"10.4172/2169-0111.1000107","DOIUrl":"https://doi.org/10.4172/2169-0111.1000107","url":null,"abstract":"Technology of genetic modifying is an alternative way to improve both the quality and the quantity of agricultural products. Genetically Modified Organism (GMO) as the product of new technology requires an excellent management strategies especially for the biosafety of the products before being released and commercialized. This study aims to determine the policy priorities in making the right decisions in order to manage a sustainable GMO while reducing the side effects of this technology to the environment and human health. The outputs of policy making based on experts justification are divided into four (4) levels, they are: Focus, Factor, Criteria and Alternative ways level. The synthesized of experts justifications on environmental, economic, social and technological factors, give a nearly equal eigen values to the previous four levels, so they are concluded in having the same priority in managing GMO. The gene flow of GMO crop to non GMO crop is the most important element to be considered with the eigen values 0.278. The increase of farmer’s income with eigen values 0.358 is considered as the most important criteria of economic factor. GMO safety to human health (0.464) is the preferred social elements. Last but not least, the human resource capability in doing biosafety test (0.580) is the most important criteria for the technology factor. Based on the alternatives compiled by the experts, law enforcement elements of the rules must be done by 0.187 eigen values-compared with other alternatives. Also based on ISM (Interpretative Structural Modelling) quadrant matrix, alternative elements are scattered into three quadrants; dependence, linkage and independent.","PeriodicalId":89733,"journal":{"name":"Advancements in genetic engineering","volume":"199 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70874265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Determinants and Perinatal Growth Phenotype 基因决定因素与围产期生长表型

Advancements in genetic engineering Pub Date : 2013-02-28 DOI: 10.4172/2169-0111.1000106

Ping-Yang Chen, Xiuquan Zhang

引用次数: 1