Blood Cells Molecules and Diseases最新文献_第8页

Unmasking the morphological alteration of erythrocytes among women suffering from PCOS 揭示多囊卵巢综合征妇女红细胞的形态改变

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2024-01-03 DOI: 10.1016/j.bcmd.2023.102821

Sutithi Dey , Ipsita Chakraborty , Payel Biswas , Ayantika Paul , Pratip Chakraborty , Rajen Haldar

{"title":"Unmasking the morphological alteration of erythrocytes among women suffering from PCOS","authors":"Sutithi Dey , Ipsita Chakraborty , Payel Biswas , Ayantika Paul , Pratip Chakraborty , Rajen Haldar","doi":"10.1016/j.bcmd.2023.102821","DOIUrl":"10.1016/j.bcmd.2023.102821","url":null,"abstract":"<div><p><span><span><span>Dyslipidemia<span> is frequently observed in polycystic ovarian syndrome (PCOS). Changes in plasma </span></span>lipid levels<span><span> potentially alter erythrocyte membrane<span> lipid composition due to lack of inbuilt </span></span>lipid synthesis<span><span> machinery. Therefore, development of morphologically altered erythrocytes in PCOS patients with dyslipidemia is expected. However, this has not been established so far. So, we took this opportunity to explore the morphological alterations among dyslipidemic PCO women. We recruited thirty-five dyslipidemic PCOS women (satisfying Rotterdam criteria, without medication) and twenty-five age-matched healthy controls. Scanning electron microscopy revealed a significant increase in the number of stomatocytes, </span>acanthocytes, and </span></span></span>echinocytes<span> in the PCO group. PCO group showed a considerable decrease in plasma antioxidant levels. Elevated lipid peroxidation, </span></span>protein carbonylation<span><span><span><span>, and decreased free thiol group in erythrocyte membrane in PCOS suggest </span>oxidative degradation of the erythrocyte membrane. Elevated intracellular </span>ROS<span> levels, increased methemoglobin formation, and a decrease in NADPH methemoglobin reductase in PCOS also indicate altered physicochemical property of hemoglobin due to oxidative overload. Additionally, these patients exhibit a rise in erythrocyte membrane cholesterol and </span></span>triglyceride<span>, which promotes the membrane to become less fluidic and less fragile. Thus, these results corroborate a potential role in altering erythrocyte morphology among dyslipidemic PCO women.</span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102821"},"PeriodicalIF":2.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139094278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of CD34 positive cell dose in donor graft on the outcomes after haploidentical peripheral blood stem cell transplantation with post-transplant cyclophosphamide – A retrospective single-center study with a Japanese cohort 供体移植物中 CD34 阳性细胞剂量对移植后环磷酰胺单倍体外周血干细胞移植结果的影响--一项以日本人为队列的单中心回顾性研究

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2023-12-30 DOI: 10.1016/j.bcmd.2023.102820

Yumiko Maruyama , Hidekazu Nishikii , Naoki Kurita , Tatsuhiro Sakamoto , Keiichiro Hattori , Yasuhito Suehara , Yasuhisa Yokoyama , Takayasu Kato , Naoshi Obara , Mamiko Sakata-Yanagimoto , Shigeru Chiba

{"title":"Impact of CD34 positive cell dose in donor graft on the outcomes after haploidentical peripheral blood stem cell transplantation with post-transplant cyclophosphamide – A retrospective single-center study with a Japanese cohort","authors":"Yumiko Maruyama , Hidekazu Nishikii , Naoki Kurita , Tatsuhiro Sakamoto , Keiichiro Hattori , Yasuhito Suehara , Yasuhisa Yokoyama , Takayasu Kato , Naoshi Obara , Mamiko Sakata-Yanagimoto , Shigeru Chiba","doi":"10.1016/j.bcmd.2023.102820","DOIUrl":"10.1016/j.bcmd.2023.102820","url":null,"abstract":"<div><h3>Background</h3><p><span>Haploidentical peripheral blood stem cell transplantation<span> (haplo-PBSCT) with post-transplant cyclophosphamide<span> (PTCy) is an important therapeutic option for patients lacking an HLA-matched donor. However, the significance of CD34</span></span></span><sup>+</sup> cell dose in grafts has not been fully elucidated.</p></div><div><h3>Objective</h3><p>We aimed to explore the impact of CD34<sup>+</sup> cell dose on outcomes after haplo-PBSCT with PTCy.</p></div><div><h3>Study Design</h3><p>We retrospectively investigated 111 consecutive patients who underwent haplo-PBSCT with PTCy or HLA-matched PBSCT from related donors.</p></div><div><h3>Results</h3><p>There were no statistically significant differences in 3-year overall survival (<em>p</em> = 0.559) or progression-free survival (<em>p</em><span> = 0.974) between haplo-PBSCT and matched PBSCT. Delayed neutrophil<span> engraftment and a lower incidence of graft-versus-host disease were observed in haplo-PBSCT. The median dose of CD34</span></span><sup>+</sup> cells was 4.9 × 10<sup>6</sup> /kg in 57 haplo-PBSCT and 4.5 × 10<sup>6</sup> /kg in 54 matched PBSCTs. Importantly, patients who underwent haplo-PBSCT with the administration of CD34<sup>+</sup> cell at a dose of ≥4.0 × 10<sup>6</sup> /kg significantly had improved OS (<em>p</em> = 0.015) and decreased incidence of disease relapse (<em>p</em> = 0.001) without increasing incidence of GVHD.</p></div><div><h3>Conclusion</h3><p>Our data suggest that a higher dose of CD34<sup>+</sup> cells in haplo-PBSCT with PTCy positively impacts the outcomes without an increase of GVHD.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102820"},"PeriodicalIF":2.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of voxelotor on murine bone marrow and peripheral blood with hematopoietic progenitor cell mobilization for gene therapy of sickle cell disease voxelotor 对用于镰状细胞病基因治疗的小鼠骨髓和外周血造血祖细胞动员的影响

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2024-01-19 DOI: 10.1016/j.bcmd.2024.102824

Avital Mendelson , Yunfeng Liu , Weili Bao , Patricia A. Shi

引用次数: 0

Bio-net dataset: AI-based diagnostic solutions using peripheral blood smear images 生物网络数据集：利用外周血涂片图像的人工智能诊断解决方案

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2024-01-04 DOI: 10.1016/j.bcmd.2024.102823

Usman Ali Shams , Isma Javed , Muhammad Fizan , Aqib Raza Shah , Ghulam Mustafa , Muhammad Zubair , Yehia Massoud , Muhammad Qasim Mehmood , Muhammad Asif Naveed

{"title":"Bio-net dataset: AI-based diagnostic solutions using peripheral blood smear images","authors":"Usman Ali Shams , Isma Javed , Muhammad Fizan , Aqib Raza Shah , Ghulam Mustafa , Muhammad Zubair , Yehia Massoud , Muhammad Qasim Mehmood , Muhammad Asif Naveed","doi":"10.1016/j.bcmd.2024.102823","DOIUrl":"10.1016/j.bcmd.2024.102823","url":null,"abstract":"<div><p><span>Peripheral blood smear examination is one of the basic steps in the evaluation of different </span>blood cells<span>. It is a confirmatory step after an automated complete blood count<span> analysis. Manual microscopy is time-consuming and requires professional laboratory expertise. Therefore, the turn-around time for peripheral smear in a health care<span> center is approximately 3–4 hours. To avoid the traditional method of manual counting under the microscope a computerized automation of peripheral blood smear examination has been adopted, which is a challenging task in medical diagnostics. In recent times, deep learning techniques have overcome the challenges associated with human microscopic evaluation of peripheral smears and this has led to reduced cost and precise diagnosis. However, their application can be significantly improved by the availability of annotated datasets. This study presents a large customized annotated blood cell dataset (named the Bio-Net dataset from healthy individuals) and blood cell detection and counting in the peripheral blood smear images. A mini-version of the dataset for specialized WBC-based image processing tasks is also equipped to classify the healthy and mature WBCs in their respective classes. An object detection algorithm called You Only Look Once (YOLO) with a refashion disposition has been trained on the novel dataset to automatically detect and classify blood cells into RBCs, WBCs, and platelets and compare the results with other publicly available datasets to highlight the versatility. In short the introduction of the Bio-Net dataset and AI-powered detection and counting offers a significant potential for advancement in biomedical research for analyzing and understanding biological data.</span></span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102823"},"PeriodicalIF":2.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139396173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regarding γ′ fibrinogen levels as a biomarker of COVID-19 respiratory disease severity 将γ'纤维蛋白原水平作为 COVID-19 呼吸系统疾病严重程度的生物标志物。

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2024-01-12 DOI: 10.1016/j.bcmd.2024.102825

Lucy Z. Kornblith , Bindhya Sadhanandhan , Sreepriya Arun , Rebecca Long , Alicia J. Johnson , Jamie Noll , C.N. Ramchand , John K. Olynyk , David H. Farrell

引用次数: 0

Regarding γ′ fibrinogen levels as a biomarker of COVID-19 respiratory disease severity 将γ′纤维蛋白原水平作为 COVID-19 呼吸系统疾病严重程度的生物标志物

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2024-01-17 DOI: 10.1016/j.bcmd.2024.102826

Sangsang Wang , Diao Yu , Junwu Zhang

引用次数: 0

Unveiling mortality risk factors in paediatric sickle cell disease patients during acute crises in the Democratic Republic of the Congo 揭示刚果民主共和国急性危机期间小儿镰状细胞病患者的死亡风险因素

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2024-01-20 DOI: 10.1016/j.bcmd.2024.102828

Paul Muteb Boma , Stéphanie Luntadila Ngimbi , Junior Makiese Kindundu , Jean Israël Wela , Nathalie Lukanke Ngoie , Valentin Mukeba Ngwamah , Sandra Mbuyi Tshiswaka , Joséphine Kalenga Monga , Jules Mulefu Panda , Bruno Bonnechère

{"title":"Unveiling mortality risk factors in paediatric sickle cell disease patients during acute crises in the Democratic Republic of the Congo","authors":"Paul Muteb Boma , Stéphanie Luntadila Ngimbi , Junior Makiese Kindundu , Jean Israël Wela , Nathalie Lukanke Ngoie , Valentin Mukeba Ngwamah , Sandra Mbuyi Tshiswaka , Joséphine Kalenga Monga , Jules Mulefu Panda , Bruno Bonnechère","doi":"10.1016/j.bcmd.2024.102828","DOIUrl":"10.1016/j.bcmd.2024.102828","url":null,"abstract":"<div><p><span><span>Sickle cell disease (SCD) is a significant health burden in the Democratic Republic of the Congo (DRC). This study aims to identify </span>predictive factors of mortality in SCD children admitted to emergency care in Lubumbashi, DRC. We performed a non-interventional cohort follow-up on SCD patients aged 0 to 16 admitted for a “true emergency”. Demographic, clinical, and biological data were collected. Univariate and multivariate </span>logistic regression<span> analyses were performed to identify significant risk factors associated with mortality. Among the 121 patients included, 24 died during the follow-up period. Univariate regression revealed age, Mikobi score, referral origin, stroke, and severe infection as significant risk factors. Multivariate analyses<span><span><span> identified Hb, WBC, SR, and </span>LDH as predictive factors of mortality. Notably, patients aged 12 to 16 years faced a higher risk, shifting the age of mortality from early to late childhood and adolescence. This study provides valuable insights into mortality risk factors for </span>paediatric SCD patients during acute crises. Early diagnosis, regular follow-up, and therapeutic education are essential to improve patient outcomes and survival rates. These findings contribute to better disease management and targeted interventions, aiming to reduce mortality associated with SCD.</span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102828"},"PeriodicalIF":2.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139508528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of red cell, plasma and blood volume: Essential components of diagnostic and research studies of oxygen transport 测量红细胞、血浆和血容量：氧气运输诊断和研究的重要组成部分

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-03-01 Epub Date: 2023-12-07 DOI: 10.1016/j.bcmd.2023.102819

Marshall A. Lichtman , Josef T. Prchal

引用次数: 0

Clonal hematopoiesis and acquired genetic abnormalities of the red cell: An historical review 克隆造血和获得性红细胞遗传异常:历史回顾。

IF 2.3 4区医学

Blood Cells Molecules and Diseases Pub Date : 2024-01-01 Epub Date: 2023-11-05 DOI: 10.1016/j.bcmd.2023.102801

Marshall A. Lichtman

引用次数: 0

Hypoferremia of inflammation: Innate host defense against infections 炎症性低铁血症:宿主对感染的先天防御