Blood Cells Molecules and Diseases最新文献

{"title":"Bio-net dataset: AI-based diagnostic solutions using peripheral blood smear images","authors":"Usman Ali Shams ,&nbsp;Isma Javed ,&nbsp;Muhammad Fizan ,&nbsp;Aqib Raza Shah ,&nbsp;Ghulam Mustafa ,&nbsp;Muhammad Zubair ,&nbsp;Yehia Massoud ,&nbsp;Muhammad Qasim Mehmood ,&nbsp;Muhammad Asif Naveed","doi":"10.1016/j.bcmd.2024.102823","DOIUrl":"10.1016/j.bcmd.2024.102823","url":null,"abstract":"<div><p><span>Peripheral blood smear examination is one of the basic steps in the evaluation of different </span>blood cells<span>. It is a confirmatory step after an automated complete blood count<span> analysis. Manual microscopy is time-consuming and requires professional laboratory expertise. Therefore, the turn-around time for peripheral smear in a health care<span> center is approximately 3–4 hours. To avoid the traditional method of manual counting under the microscope a computerized automation of peripheral blood smear examination has been adopted, which is a challenging task in medical diagnostics. In recent times, deep learning techniques have overcome the challenges associated with human microscopic evaluation of peripheral smears and this has led to reduced cost and precise diagnosis. However, their application can be significantly improved by the availability of annotated datasets. This study presents a large customized annotated blood cell dataset (named the Bio-Net dataset from healthy individuals) and blood cell detection and counting in the peripheral blood smear images. A mini-version of the dataset for specialized WBC-based image processing tasks is also equipped to classify the healthy and mature WBCs in their respective classes. An object detection algorithm called You Only Look Once (YOLO) with a refashion disposition has been trained on the novel dataset to automatically detect and classify blood cells into RBCs, WBCs, and platelets and compare the results with other publicly available datasets to highlight the versatility. In short the introduction of the Bio-Net dataset and AI-powered detection and counting offers a significant potential for advancement in biomedical research for analyzing and understanding biological data.</span></span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102823"},"PeriodicalIF":2.3,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139396173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unmasking the morphological alteration of erythrocytes among women suffering from PCOS","authors":"Sutithi Dey ,&nbsp;Ipsita Chakraborty ,&nbsp;Payel Biswas ,&nbsp;Ayantika Paul ,&nbsp;Pratip Chakraborty ,&nbsp;Rajen Haldar","doi":"10.1016/j.bcmd.2023.102821","DOIUrl":"10.1016/j.bcmd.2023.102821","url":null,"abstract":"<div><p><span><span><span>Dyslipidemia<span> is frequently observed in polycystic ovarian syndrome (PCOS). Changes in plasma </span></span>lipid levels<span><span> potentially alter erythrocyte membrane<span> lipid composition due to lack of inbuilt </span></span>lipid synthesis<span><span> machinery. Therefore, development of morphologically altered erythrocytes in PCOS patients with dyslipidemia is expected. However, this has not been established so far. So, we took this opportunity to explore the morphological alterations among dyslipidemic PCO women. We recruited thirty-five dyslipidemic PCOS women (satisfying Rotterdam criteria, without medication) and twenty-five age-matched healthy controls. Scanning electron microscopy revealed a significant increase in the number of stomatocytes, </span>acanthocytes, and </span></span></span>echinocytes<span> in the PCO group. PCO group showed a considerable decrease in plasma antioxidant levels. Elevated lipid peroxidation, </span></span>protein carbonylation<span><span><span><span>, and decreased free thiol group in erythrocyte membrane in PCOS suggest </span>oxidative degradation of the erythrocyte membrane. Elevated intracellular </span>ROS<span> levels, increased methemoglobin formation, and a decrease in NADPH methemoglobin reductase in PCOS also indicate altered physicochemical property of hemoglobin due to oxidative overload. Additionally, these patients exhibit a rise in erythrocyte membrane cholesterol and </span></span>triglyceride<span>, which promotes the membrane to become less fluidic and less fragile. Thus, these results corroborate a potential role in altering erythrocyte morphology among dyslipidemic PCO women.</span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102821"},"PeriodicalIF":2.3,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139094278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of CD34 positive cell dose in donor graft on the outcomes after haploidentical peripheral blood stem cell transplantation with post-transplant cyclophosphamide – A retrospective single-center study with a Japanese cohort","authors":"Yumiko Maruyama ,&nbsp;Hidekazu Nishikii ,&nbsp;Naoki Kurita ,&nbsp;Tatsuhiro Sakamoto ,&nbsp;Keiichiro Hattori ,&nbsp;Yasuhito Suehara ,&nbsp;Yasuhisa Yokoyama ,&nbsp;Takayasu Kato ,&nbsp;Naoshi Obara ,&nbsp;Mamiko Sakata-Yanagimoto ,&nbsp;Shigeru Chiba","doi":"10.1016/j.bcmd.2023.102820","DOIUrl":"10.1016/j.bcmd.2023.102820","url":null,"abstract":"<div><h3>Background</h3><p><span>Haploidentical peripheral blood stem cell transplantation<span> (haplo-PBSCT) with post-transplant cyclophosphamide<span> (PTCy) is an important therapeutic option for patients lacking an HLA-matched donor. However, the significance of CD34</span></span></span>⁺ cell dose in grafts has not been fully elucidated.</p></div><div><h3>Objective</h3><p>We aimed to explore the impact of CD34⁺ cell dose on outcomes after haplo-PBSCT with PTCy.</p></div><div><h3>Study Design</h3><p>We retrospectively investigated 111 consecutive patients who underwent haplo-PBSCT with PTCy or HLA-matched PBSCT from related donors.</p></div><div><h3>Results</h3><p>There were no statistically significant differences in 3-year overall survival (<em>p</em> = 0.559) or progression-free survival (<em>p</em><span> = 0.974) between haplo-PBSCT and matched PBSCT. Delayed neutrophil<span> engraftment and a lower incidence of graft-versus-host disease were observed in haplo-PBSCT. The median dose of CD34</span></span>⁺ cells was 4.9 × 10⁶ /kg in 57 haplo-PBSCT and 4.5 × 10⁶ /kg in 54 matched PBSCTs. Importantly, patients who underwent haplo-PBSCT with the administration of CD34⁺ cell at a dose of ≥4.0 × 10⁶ /kg significantly had improved OS (<em>p</em> = 0.015) and decreased incidence of disease relapse (<em>p</em> = 0.001) without increasing incidence of GVHD.</p></div><div><h3>Conclusion</h3><p>Our data suggest that a higher dose of CD34⁺ cells in haplo-PBSCT with PTCy positively impacts the outcomes without an increase of GVHD.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102820"},"PeriodicalIF":2.3,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of red cell, plasma and blood volume: Essential components of diagnostic and research studies of oxygen transport","authors":"Marshall A. Lichtman ,&nbsp;Josef T. Prchal","doi":"10.1016/j.bcmd.2023.102819","DOIUrl":"10.1016/j.bcmd.2023.102819","url":null,"abstract":"","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"105 ","pages":"Article 102819"},"PeriodicalIF":2.3,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138555924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal hematopoiesis and acquired genetic abnormalities of the red cell: An historical review","authors":"Marshall A. Lichtman","doi":"10.1016/j.bcmd.2023.102801","DOIUrl":"10.1016/j.bcmd.2023.102801","url":null,"abstract":"<div><p>Several syndromes affecting the red cell that mimic those induced by germline mutations may result from a somatic mutation that accompanies a myeloid malignancy. These syndromes are most notable in cases of myelodysplastic syndrome, but they are not limited to any one category of myeloid neoplasm. Their occurrence in males exceed the male predominance that is evident in myeloid neoplasms. The syndromes include disorders of globin chain synthesis (α- and β-thalassemia), heme synthesis (erythropoietic porphyria and erythropoietic uroporphyria), red cell membrane structure (elliptocytosis and spherocytosis), red cell enzyme activity (pyruvate kinase deficiency, glucose-6-phosphate dehydrogenase deficiency) and lowered expression of red cell ABO blood group antigens. This historical review describes the path to uncovering these acquired syndromes and their causal somatic mutations, where known. These syndromes often go unrecognized because of the dominant concern of the primary neoplasm. They may add to the healthcare needs of the patient.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"104 ","pages":"Article 102801"},"PeriodicalIF":2.3,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89716868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium flux alterations in erythrocytes from sickle cell mice: The relevance of mean corpuscular volume","authors":"Luis E.F. Almeida,&nbsp;Meghann L. Smith,&nbsp;Sayuri Kamimura,&nbsp;Sebastian Vogel,&nbsp;Zenaide M.N. Quezado","doi":"10.1016/j.bcmd.2023.102800","DOIUrl":"10.1016/j.bcmd.2023.102800","url":null,"abstract":"<div><p>Red blood cells (RBC) from patients with sickle cell disease (SCD) have elevated calcium levels at baseline, which are further elevated upon deoxygenation. Here we examined baseline calcium levels and calcium flux in RBCs from a mouse model of SCD mice. We found that akin to humans with SCD, sickle (HbSS) Townes mice, have higher baseline levels and increased calcium flux in RBCs compared to control (HbAA) animals. As HbSS mice, unlike humans with SCD, have high mean corpuscular volume compared with HbAA, we highlight the importance of adjusting biochemical results to number of RBCs rather than hematocrit during the analysis and interpretation of the results. Our findings add to the face validity of humanized sickle cell mice and support its use for studies of RBC calcium flux in SCD.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"104 ","pages":"Article 102800"},"PeriodicalIF":2.3,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89716867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antineoplastic effects of pharmacological inhibitors of aurora kinases in CSF3RT618I-driven cells","authors":"Natália Sudan Parducci ,&nbsp;Anali Del Milagro Bernabe Garnique ,&nbsp;Keli Lima ,&nbsp;Jorge Antonio Elias Godoy Carlos ,&nbsp;Natasha Peixoto Fonseca ,&nbsp;Lívia Bassani Lins de Miranda ,&nbsp;Bruna Oliveira de Almeida ,&nbsp;Eduardo Magalhães Rego ,&nbsp;Fabiola Traina ,&nbsp;João Agostinho Machado-Neto","doi":"10.1016/j.bcmd.2023.102799","DOIUrl":"https://doi.org/10.1016/j.bcmd.2023.102799","url":null,"abstract":"<div><p><span>Myeloproliferative neoplasms<span><span> (MPN) are consolidated as a relevant group of diseases<span> derived from the malfunction of the hematopoiesis process and have as a particular attribute the increased proliferation of myeloid </span></span>lineage<span>. Among these, chronic neutrophilic leukemia (CNL) is distinguished, caused by the T618I mutation of the </span></span></span><em>CSF3R</em><span> gene, a trait that generates ligand-independent receptor activation and downstream JAK2/STAT signaling. Previous studies reported that mutations in BCR::ABL1 and JAK2</span>^V617F<span> increased the expression of the aurora kinase A<span> (AURKA) and B (AURKB) in Ba/F3 cells and their pharmacological inhibition displays antineoplastic effects in human BCR::ABL1 and JAK2</span></span>^V617F positive cells. Delimiting the current scenario, aspects related to the AURKA and AURKB as a potential target in CSF3R^T618I<span>-driven models is little known. In the present study, the cellular and molecular effects of pharmacological inhibitors of aurora kinases, such as aurora A inhibitor I, AZD1152-HQPA, and reversine, were evaluated in Ba/F3 expressing the CSF3R</span>^T618I<span><span><span><span><span> mutation. AZD1152-HQPA and reversine demonstrated antineoplastic potential, causing a decrease in cell viability, </span>clonogenicity, and proliferative capacity. At molecular levels, all inhibitors reduced </span>histone H3<span> phosphorylation, aurora A inhibitor I and reversine reduced STAT5 phosphorylation, and AZD1152-HQPA and reversine induced </span></span>PARP1<span> cleavage and γH2AX expression. Reversine more efficiently modulated genes associated with cell cycle and apoptosis compared to other </span></span>drugs. In summary, our findings shed new insights into the use of AURKB inhibitors in the context of CNL.</span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"104 ","pages":"Article 102799"},"PeriodicalIF":2.3,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49821171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of PKC in X-ray-induced megakaryocyte apoptosis and thrombocytopenia","authors":"Fanbi Meng ,&nbsp;Shuang Chen ,&nbsp;Chunliang Liu ,&nbsp;Muhammad Shoaib Khan,&nbsp;Yan Yan,&nbsp;Jun Wan,&nbsp;Yue Xia,&nbsp;Chenglin Sun,&nbsp;Mengnan Yang,&nbsp;Renping Hu,&nbsp;Kesheng Dai","doi":"10.1016/j.bcmd.2023.102798","DOIUrl":"10.1016/j.bcmd.2023.102798","url":null,"abstract":"<div><p><span>Thrombocytopenia<span> is a critical complication after radiation therapy and exposure. Dysfunction of megakaryocyte<span><span> development and platelet production<span> are key pathophysiological stages in ionizing radiation (IR)-induced thrombocytopenia. </span></span>Protein kinase C<span> (PKC) plays an important role in regulating megakaryocyte development and platelet production. However, it remains unclear how PKC regulates IR-induced megakaryocyte apoptosis<span><span><span>. In this study, we found that pretreatment of PKC pan-inhibitor Go6983 delayed IR-induced megakaryocyte apoptosis, and inhibited IR-induced </span>mitochondrial membrane potential and </span>ROS production in CMK cells. Moreover, suppressing PKC activation inhibited cleaved caspase3 expression and reduced p38 phosphorylation levels, and IR-induced PKC activation might be regulated by p53. </span></span></span></span></span><em>In vivo</em><span> experiments confirmed that Go6983 promoted platelet count<span><span> recovery after 21 days of 3 Gy total body irradiation. Furthermore, Go6983 reduced megakaryocyte apoptosis, increased the number of megakaryocyte and </span>polyploid formation in bone marrow, and improved the survival rate of 6 Gy total body irradiation. In conclusion, our results provided a potential therapeutic target for IR-induced thrombocytopenia.</span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"104 ","pages":"Article 102798"},"PeriodicalIF":2.3,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of a patient with homozygous hemoglobin Ernz: Evidence to support a non-pathogenic variant","authors":"Zohreh Shojaei ,&nbsp;Maryam Abiri ,&nbsp;Fatemeh Zafarghandi Motlagh ,&nbsp;Masoume Amini ,&nbsp;Samira Dabbagh Bagheri ,&nbsp;Sadaf Asnavandi ,&nbsp;Sedighe Asadi ,&nbsp;Hamideh Bagherian ,&nbsp;Sirous Zeinali","doi":"10.1016/j.bcmd.2023.102797","DOIUrl":"10.1016/j.bcmd.2023.102797","url":null,"abstract":"<div><p>Hemoglobin Ernz (Hb Ernz) is a missense variant in β-globin caused by a Threonine<span><span> to Asparagine substitution at the 123rd </span>amino acid<span><span> position and HBB c.371C &gt; A in gene level. Hb Ernz has been classified as Uncertain Significance (VUS) by ACMG due to limited reports and the absence of any homozygote<span> genotypes. In our study, we found eight cases of Hb Ernz by DNA sequencing of the β-globin gene during &gt;20 years of </span></span>Thalassemia Screening in individuals with borderline hematological parameters who were possible carriers of thalassemia or their spouses. We also report the first homozygote variant of Hb Ernz. Our findings suggest that the changes in hematological parameters observed in individuals with Hb Ernz are likely due to α-globin gene mutations rather than Hb Ernz itself. These findings support the reclassification of Hb Ernz as a benign variant in variant classification.</span></span></p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"104 ","pages":"Article 102797"},"PeriodicalIF":2.3,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41190220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting of Calbindin 1 rescues erythropoiesis in a human model of Diamond Blackfan anemia","authors":"Nan Wang ,&nbsp;Corinne LaVasseur ,&nbsp;Rao Riaz ,&nbsp;Julien Papoin ,&nbsp;Lionel Blanc ,&nbsp;Anupama Narla","doi":"10.1016/j.bcmd.2023.102759","DOIUrl":"10.1016/j.bcmd.2023.102759","url":null,"abstract":"<div><p><span>Diamond Blackfan anemia<span><span> (DBA) is an inherited bone marrow failure syndrome<span> characterized by congenital anomalies<span>, cancer predisposition and a severe hypo-proliferative anemia. It was the first disease linked to ribosomal dysfunction and &gt;70 % of patients have been identified to have a </span></span></span>haploinsufficiency<span> of a ribosomal protein (RP) gene, with </span></span></span><em>RPS19</em><span><span><span> being the most common mutation. There is significant variability within the disease in terms of phenotype as well as response to therapy suggesting that other genes contribute to the pathophysiology and potential management of this disease. To explore these questions, we performed a genome-wide </span>CRISPR screen in a </span>cellular model<span> of DBA and identified Calbindin 1<span> (CALB1), a member of the calcium-binding superfamily, as a potential modifier of the disordered erythropoiesis in DBA. We used human derived CD34+ cells cultured in erythroid stimulating media with knockdown of </span></span></span><em>RPS19</em> as a model for DBA to study the effects of CALB1. We found that knockdown of CALB1 in this DBA model promoted erythroid maturation. We also noted effects of CALB1 knockdown on cell cycle. Taken together, our results reveal CALB1 is a novel regulator of human erythropoiesis and has implications for using CALB1 as a novel therapeutic target in DBA.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":"102 ","pages":"Article 102759"},"PeriodicalIF":2.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9769247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}