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Real-Time PCR assay for detection of candida parap-silosis and candida orthopsilosis Real-Time PCR检测假丝酵母菌副丝酵母菌和假丝酵母菌矫形丝酵母菌
The journal of bioscience and medicine Pub Date : 2012-06-24 DOI: 10.5780/jbm2012
Giek Far Chan, N. Hasan, S. Sinniah
{"title":"Real-Time PCR assay for detection of candida parap-silosis and candida orthopsilosis","authors":"Giek Far Chan, N. Hasan, S. Sinniah","doi":"10.5780/jbm2012","DOIUrl":"https://doi.org/10.5780/jbm2012","url":null,"abstract":"C. parapsilosis and C. orthopsilosis emerged as fungal pathogen with significant worldwide prevalence, particularly in causing nosocomial and skin infections. In this study, we aimed to develop molecular assay based on real-time PCR for sensitive and accurate detection of C. parapsilosis and C. orthopsilosis . A pair of primers that specifically target on both of these yeast species was designed and real-time PCR amplification assay was optimized using EvaGreen as the DNA binding dye. The optimized assay could detect and quantify up to 1 pg concentration of C. parapsilosis and C. orthopsilosis DNA with amplification efficiency of 104% and 103%, respectively. Both the designed primers and the quantitative assay will have a great potential as molecular diagnosis tool for early detection of fungal infection caused by either C. parapsilosis or C. orthopsilosis , which merits future clinical study prior to use in diagnosis.","PeriodicalId":89629,"journal":{"name":"The journal of bioscience and medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71006307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Successful Recruitment of Centenarians for Post-Mortem Brain Donation: Results from the Georgia Centenarian Study. 成功招募百岁老人进行死后脑捐赠:来自乔治亚州百岁老人研究的结果。
The journal of bioscience and medicine Pub Date : 2012-01-01
Kathy Shaw, Marla Gearing, Adam Davey, Molly Burgess, Leonard W Poon, Peter Martin, Robert C Green
{"title":"Successful Recruitment of Centenarians for Post-Mortem Brain Donation: Results from the Georgia Centenarian Study.","authors":"Kathy Shaw,&nbsp;Marla Gearing,&nbsp;Adam Davey,&nbsp;Molly Burgess,&nbsp;Leonard W Poon,&nbsp;Peter Martin,&nbsp;Robert C Green","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Brain donation and neuropathological examination of brain tissues is the only way to obtain definitive diagnostic information on research subjects enrolled in aging studies. We investigated predictors of brain donation in a population-based study of centenarians in Phase III of the Georgia Centenarian Study (GCS).</p><p><strong>Methods: </strong>Sixty-six individuals (mean age = 100.6 years, 91% female, 20% African American) were successfully recruited from the core sample of 244 individuals residing in 44 counties of Northeast Georgia to provide brain donation.</p><p><strong>Results: </strong>Bivariate (t-tests, chi-square tests) and multivariate analyses (logistic regression) showed no significant differences between donors and non-donors across a wide range of demographic, religious, personality, cognitive and physical functioning characteristics.</p><p><strong>Conclusions: </strong>We succeeded in recruiting a diverse, population-based sample of centenarians for brain donation. Our findings also suggest that barriers to brain donation reported in other studies may have less impact in these exceptional survivors.</p>","PeriodicalId":89629,"journal":{"name":"The journal of bioscience and medicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763720/pdf/nihms499532.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31714925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On the Mechanism of T cell receptor down-modulation and its physiological significance. T细胞受体下调的机制及其生理意义。
The journal of bioscience and medicine Pub Date : 2011-09-13 DOI: 10.5780/JBM2011.5
D. Escors, C. Bricogne, F. Arce, G. Kochan, K. Karwacz
{"title":"On the Mechanism of T cell receptor down-modulation and its physiological significance.","authors":"D. Escors, C. Bricogne, F. Arce, G. Kochan, K. Karwacz","doi":"10.5780/JBM2011.5","DOIUrl":"https://doi.org/10.5780/JBM2011.5","url":null,"abstract":"Effective, long-lasting immune responses largely depend upon T cell reponses. Antigen-specific T lymphocytes are activated and differentiate into effector T cells after antigen presentation by professional antigen presenting cells (APCs). However, T cell responses are tightly regulated to prevent T cell hyperactivation which may end up in autoimmune pathology. One of these regulatory mechanisms is ligand-induced TCR down-modulation, a process by which TCRs are removed from the T cell surface shortly after engagement with their cognate antigenic peptide associated to MHC molecules on the APC. TCR down-modulation is a complicated process. Here we briefly describe the three main models that attempt to clarify this mechanism in the context of T cell activation and function.","PeriodicalId":89629,"journal":{"name":"The journal of bioscience and medicine","volume":"1 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71006120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
On the Mechanism of T cell receptor down-modulation and its physiological significance. 关于 T 细胞受体下调的机制及其生理意义。
The journal of bioscience and medicine Pub Date : 2011-01-01
David Escors, Christopher Bricogne, Frederick Arce, Grazyna Kochan, Katarzyna Karwacz
{"title":"On the Mechanism of T cell receptor down-modulation and its physiological significance.","authors":"David Escors, Christopher Bricogne, Frederick Arce, Grazyna Kochan, Katarzyna Karwacz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Effective, long-lasting immune responses largely depend upon T cell reponses. Antigen-specific T lymphocytes are activated and differentiate into effector T cells after antigen presentation by professional antigen presenting cells (APCs). However, T cell responses are tightly regulated to prevent T cell hyperactivation which may end up in autoimmune pathology. One of these regulatory mechanisms is ligand-induced TCR down-modulation, a process by which TCRs are removed from the T cell surface shortly after engagement with their cognate antigenic peptide associated to MHC molecules on the APC. TCR down-modulation is a complicated process. Here we briefly describe the three main models that attempt to clarify this mechanism in the context of T cell activation and function.</p>","PeriodicalId":89629,"journal":{"name":"The journal of bioscience and medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272427/pdf/ukmss-36879.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30446464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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