Nanomedicine : design and applications of magnetic nanomaterials, nanosensors, and nanosystems最新文献

筛选
英文 中文
NANOSTRUCTURED PROBES FOR IN VIVO GENE DETECTION. 用于体内基因检测的纳米结构探针。
Nanomedicine : design and applications of magnetic nanomaterials, nanosensors, and nanosystems Pub Date : 2010-01-01 DOI: 10.1002/9783527628155.nanotech054
Gang Bao, Phillip Santangelo, Nitin Nitin, Won Jong Rhee
{"title":"NANOSTRUCTURED PROBES FOR IN VIVO GENE DETECTION.","authors":"Gang Bao, Phillip Santangelo, Nitin Nitin, Won Jong Rhee","doi":"10.1002/9783527628155.nanotech054","DOIUrl":"10.1002/9783527628155.nanotech054","url":null,"abstract":"<p><p>The ability to visualize in real-time the expression dynamics and localization of specific RNAs in vivo offers tremendous opportunities for biological and disease studies including cancer detection. However, quantitative methods such as real-time PCR and DNA microarrays rely on the use of cell lysates thus not able to obtain important spatial and temporal information. Fluorescence proteins and other reporter systems cannot image endogenous RNA in living cells. Fluorescence in situ hybridization (FISH) assays require washing to achieve specificity, therefore can only be used with fixed cells. Here we review the recent development of nanostructured probes for living cell RNA detection, and discuss the biological and engineering issues and challenges of quantifying gene expression in vivo. In particular, we describe methods that use oligonucleotide probes, combined with novel delivery strategies, to image the relative level, localization and dynamics of RNA in live cells. Examples of detecting endogenous mRNAs, as well as imaging their subcellular localization are given to illustrate the biological applications, and issues in probe design, delivery and target accessibility are discussed. The nanostructured probes promise to open new and exciting opportunities in sensitive gene detection for a wide range of biological and medical applications.</p>","PeriodicalId":89605,"journal":{"name":"Nanomedicine : design and applications of magnetic nanomaterials, nanosensors, and nanosystems","volume":" ","pages":"143-165"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226718/pdf/nihms-132479.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30300976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信