Biomedica biochimica acta最新文献

But what makes it doctoral?: taking on the traditionalists: interdisciplinary, practice-led doctoral research in the creative industries: a case study in academic politics, research, rigour and relevance 但是什么让它成为博士呢?创意产业中跨学科、以实践为主导的博士研究:学术政治、研究、严谨性和相关性的案例研究

Biomedica biochimica acta Pub Date : 2010-10-01 DOI: 10.18848/1833-1882/cgp/v05i07/51778

Christiaan Willems

{"title":"But what makes it doctoral?: taking on the traditionalists: interdisciplinary, practice-led doctoral research in the creative industries: a case study in academic politics, research, rigour and relevance","authors":"Christiaan Willems","doi":"10.18848/1833-1882/cgp/v05i07/51778","DOIUrl":"https://doi.org/10.18848/1833-1882/cgp/v05i07/51778","url":null,"abstract":"This paper examines a Doctoral journey of interdisciplinary exploration, explication, examination...and exasperation. In choosing to pursue a practice-led doctorate I had determined from the outset that 'writing 100,000 words that only two people ever read', was not something which interested me. Hence, the oft-asked question of 'what kind of doctorate' I was engaged in, consistently elicited the response, 'a useful one'. In order to satisfy my own imperatives of authenticity and usefulness, my doctoral research had to clearly demonstrate relevance to; productively inform; engage with; and add value to: wider professional field(s) of practice; students in the university courses I teach; and the broader community - not just the academic community. Consequently, over the course of my research, the question, 'But what makes it Doctoral?' consistently resounded and resonated. Answering that question, to satisfy not only the traditionalists asking it but, perhaps surprisingly, some academic innovators - and more particularly, myself as researcher - revealed academic/political inconsistencies and issues which challenged both the fundamental assumptions and actuality of practice-led research. \u0000This paper examines some of those inconsistencies, issues and challenges and provides at least one possible answer to the question: 'But what makes it Doctoral?'","PeriodicalId":8948,"journal":{"name":"Biomedica biochimica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74934351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

How Homelessness Compromises the Exercise of the Rights of Citizenship in Australia 无家可归如何影响澳大利亚公民权利的行使

Biomedica biochimica acta Pub Date : 2010-01-01 DOI: 10.18848/1833-1882/cgp/v05i02/51600

E. Schindeler

引用次数: 0

XIth International Karlsburg Symposium on Problems of Diabetes. September 19-21, 1983. 第十一届国际卡尔堡糖尿病问题研讨会。1983年9月19日至21日。

Biomedica biochimica acta Pub Date : 2009-07-17 DOI: 10.1055/S-0029-1210320

H. Bibergeil, U. Fischer, D. Michaelis, U. Poser

引用次数: 0

Stepwise activation mechanisms of the precursors of matrix metalloproteinases 1 (tissue collagenase) and 3 (stromelysin). 基质金属蛋白酶1(组织胶原酶)和基质金属蛋白酶3(基质溶解素)前体的逐步激活机制。

Biomedica biochimica acta Pub Date : 1991-01-01

H Nagase, K Suzuki, J J Enghild, G Salvesen

{"title":"Stepwise activation mechanisms of the precursors of matrix metalloproteinases 1 (tissue collagenase) and 3 (stromelysin).","authors":"H Nagase, K Suzuki, J J Enghild, G Salvesen","doi":"","DOIUrl":"","url":null,"abstract":"Two zymogens of matrix metalloproteinases (MMPs), proMMP-1 (tissue procollagenase) and proMMP-3 (prostromelysin) were isolated from the culture medium of human rheumatoid synovial fibroplasts and their activation mechanisms by proteinases and 4-aminophenylmercuric acetate (APMA) were studied by kinetic and sequence analyses. Both zymogens were activated by unique stepwise activation mechanisms through which sequential processing events occur in the propeptide regions. The initial cleavage sites attacked by activator proteinases are located in the middle of the propeptides at Glu33-Lys-Arg-Arg-Asn37 in proMMP-1 and Phe34-Val-Arg-Arg-Lys-Asp39 in proMMP-3. The initial products of proMMP-1 generated by proteinases then undergo further autocleavage of the Thr64-Leu65 bond. The treatment of proMMP-1 and proMMP-3 with APMA results in the intramolecular cleavage of the Val67-Met68 and Glu68-Val69 bonds, respectively. The removal of a portion of propeptides results in conformational changes around the Gln80-Phe81 and His82-Phe83 bonds in respective intermediates of MMP-1 and MMP-3 and render them to rapid specific cleavage by MMP-3 to generate stable, fully active enzymes.","PeriodicalId":8948,"journal":{"name":"Biomedica biochimica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12832282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukodiapedesis, release of PMN leukocyte proteinases and activation of PMNL procollagenase. 白细胞脱落，PMN白细胞蛋白酶的释放和PMNL前胶原酶的激活。

Biomedica biochimica acta Pub Date : 1991-01-01

H Tschesche, B Bakowski, A Schettler, V Knäuper, H Reinke

{"title":"Leukodiapedesis, release of PMN leukocyte proteinases and activation of PMNL procollagenase.","authors":"H Tschesche, B Bakowski, A Schettler, V Knäuper, H Reinke","doi":"","DOIUrl":"","url":null,"abstract":"Human polymorphonuclear leukocytes mainly protect the body against invading microorganisms. Therefore, cells must migrate to the site of infection, whereby they traverse the endothelial cell layer of the blood vessels, basement membranes and the connective tissue. We investigated the unknown mechanism of penetration of basement membranes by scanning electron microscopy. Our findings indicate a three-step mechanism--adhesion, locally limited proteolytic degradation of matrix components and mechanical loosening of the matrix. Finally, cells locomote in the stroma tissue to the site of infection. These processes are induced by formyl-peptides amongst others. In addition, an increased release of collagenase induced by formyl-peptides was observed. In contrast, incubation with prostaglandin F2 alpha led to significantly higher levels of secreted collagenase, while elastase failed to be detected in the supernatant of stimulated cells. Since it has been shown that collagenase is secreted in a latent form, we have focussed our attention on the activation mechanism of the proenzyme, which was investigated by determination of the N-terminal sequences of intermediate and final, activated forms.","PeriodicalId":8948,"journal":{"name":"Biomedica biochimica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12832283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of alpha and beta-glycopyranosyl-serine derivatives by enzymic transglycosylation. 酶转糖基化法合成α和β -甘氨酰丝氨酸衍生物。

Biomedica biochimica acta Pub Date : 1991-01-01

D Cantacuzene, S Attal, S Bay

引用次数: 0

A novel membrane-bound serine esterase in human T4(+)-lymphocytes is a binding protein of envelope glycoprotein gp120 of HIV-1. 人T4(+)淋巴细胞中一种新的膜结合丝氨酸酯酶是HIV-1包膜糖蛋白gp120的结合蛋白。

Biomedica biochimica acta Pub Date : 1991-01-01

H Kido, A Fukutomi, N Katunuma

{"title":"A novel membrane-bound serine esterase in human T4(+)-lymphocytes is a binding protein of envelope glycoprotein gp120 of HIV-1.","authors":"H Kido, A Fukutomi, N Katunuma","doi":"","DOIUrl":"","url":null,"abstract":"A novel membrane-bound serine esterase, named tryptase TL2, which is immunologically reactive with the antibody inhibiting induction of syncytia by human immunodeficiency virus-1 (HIV-1) (HATTORI, T., KOITO, A., TAKATSUKI, K., KIDO, H., and KATUNUMA, N., 1989, FEBS Lett., 248, 48-52), has been purified from a human T4+ lymphocyte clone. The enzyme has a molecular mass of 198 +/- 15 kDa, and is composed of two subunits of 32 kDa and four subunits of 28 kDa. The enzyme was strongly inhibited by the envelope glycoprotein gp120 of HIV-1, by synthetic peptides of V3 domains of gp120 s with the sequence GPGR in their center, which correspond to the principal neutralizing epitopes of the gp120s of various HIV-1 strains, by Kunitz-type inhibitors with the sequence GPCR in their active site, such as trypstatin, H130, and [Arg15, Glu52] aprotinin and by the microbial inhibitors leupeptin and antipain. This enzyme was specifically bound to the inhibitor V3 domain of gp120 of HIV-1, and this binding was blocked by the inhibitors of tryptase TL2, with a central motif GPCR or GPGR sequence in their center, but not by leupeptin and antipain without the motif. These findings suggest that tryptase TL2 is important in target site recognition and binding of HIV-1 in co-operation with CD4 receptor in the initial process of HIV-1 infection.","PeriodicalId":8948,"journal":{"name":"Biomedica biochimica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12851520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoaffinity purification of human alpha-fetoprotein (AFP) using monoclonal antibodies. 利用单克隆抗体免疫亲和纯化人甲胎蛋白(AFP)。

Biomedica biochimica acta Pub Date : 1991-01-01

G Kaiser, B Micheel

引用次数: 0

The 5'-untranslated region of p23 mRNA from the Ehrlich ascites tumor is involved in translation control of the growth related protein p23. 来自埃利希腹水肿瘤的p23 mRNA的5'-未翻译区参与了生长相关蛋白p23的翻译控制。

Biomedica biochimica acta Pub Date : 1991-01-01

H Böhm, B Gross, M Gaestel, U A Bommer, G Ryffel, H Bielka

引用次数: 0

The ubiquitin-activating enzyme is required for lysosomal degradation of cellular proteins under stress. 泛素激活酶是在应激条件下溶酶体降解细胞蛋白所必需的。

Biomedica biochimica acta Pub Date : 1991-01-01

A Ciechanover, R Gropper, A L Schwartz

{"title":"The ubiquitin-activating enzyme is required for lysosomal degradation of cellular proteins under stress.","authors":"A Ciechanover, R Gropper, A L Schwartz","doi":"","DOIUrl":"","url":null,"abstract":"ts85, a cell-line that harbors a mutant thermolabile ubiquitin-activating enzyme, E1, fails to degrade short-lived proteins at the restrictive temperature (Ciechanover, A., Finley, D., and Varshavsky, A. (1984) Cell 37, 57-66). It is not known whether the ubiquitin system is also involved in the degradation of long-lived proteins. In the present study we show that upon shifting the mutant cells to the restrictive temperature, there is no change in the rate of degradation of long-lived proteins. In contrast, shifting the wild-type cells (FM3A) to the high temperature is accompanied by a 2-fold increase in the rate of proteolysis of this group of proteins. This heat-induced accelerated degradation can be completely inhibited by NH4Cl and chloroquine. Similarly, exposure of the cells to starvation, a stimulus that activates the autophagic-lysosomal pathway, has no effect on the degradation of long-lived proteins in the mutant cells following inactivation of E1. Under the same conditions, the degradation rate in the wild-type cells increases almost 4-fold. A revertant of the ts85 cells behaved in a similar manner to the wild-type cells. Analogous results were obtained using a different cell line that also harbors a thermolabile E1 (ts20) (Kulka, R. G. et al. (1988) J. Biol. Chem. 263, 15726-15731). Cycloheximide and 3-methyladenine, inhibitors of formation of autophagic vacuoles, suppress the heat-induced accelerated degradation in the wild-type cells. Taken together, the results suggest that: 1. heat stress induces enhanced degradation of intracellular proteins, 2. the process occurs most probably in autophagic vacuoles, 3. activation of ubiquitin is required for enhanced degradation to occur, and 4. the activation is involved most probably in formation of the autophagic vacuoles.","PeriodicalId":8948,"journal":{"name":"Biomedica biochimica acta","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12963269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0