Mobile genetic elements最新文献

tRNAs as primers and inhibitors of retrotransposons. trna作为反转录转座子的引物和抑制剂。

Mobile genetic elements Pub Date : 2017-11-01 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1393490

German Martinez

引用次数: 8

LINE-1 in response to exposure to ionizing radiation. LINE-1对电离辐射的反应。

Mobile genetic elements Pub Date : 2017-10-19 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1393491

Igor Koturbash

引用次数: 6

Staphylococci on ICE: Overlooked agents of horizontal gene transfer. ICE上的葡萄球菌:被忽视的水平基因转移因子。

Mobile genetic elements Pub Date : 2017-08-22 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1368433

Emily A Sansevere, D Ashley Robinson

{"title":"Staphylococci on ICE: Overlooked agents of horizontal gene transfer.","authors":"Emily A Sansevere, D Ashley Robinson","doi":"10.1080/2159256X.2017.1368433","DOIUrl":"https://doi.org/10.1080/2159256X.2017.1368433","url":null,"abstract":"Horizontal gene transfer plays a significant role in spreading antimicrobial resistance and virulence genes throughout the genus Staphylococcus, which includes species of clinical relevance to humans and animals. While phages and plasmids are the most well-studied agents of horizontal gene transfer in staphylococci, the contribution of integrative conjugative elements (ICEs) has been mostly overlooked. Experimental work demonstrating the activity of ICEs in staphylococci remained frozen for years after initial work in the 1980s that showed Tn916 was capable of transfer from Enterococcus to Staphylococcus. However, recent work has begun to thaw this field. To date, 2 families of ICEs have been identified among staphylococci - Tn916 that includes the Tn5801 subfamily, and ICE6013 that includes at least 7 subfamilies. Both Tn5801 and ICE6013 commonly occur in clinical strains of S. aureus. Tn5801 is the most studied of the Tn916 family elements in staphylococci and encodes tetracycline resistance and a protein that, when expressed in Escherichia coli, inhibits restriction barriers to incoming DNA. ICE6013 is among the shortest known ICEs, but it still includes many uncharacterized open reading frames. This element uses an IS30-like transposase as its recombinase, providing some versatility in integration sites. ICE6013 also conjugatively transfers among receptive S. aureus strains at relatively higher frequency than Tn5801. Continued study of these mobile genetic elements may reveal the full extent to which ICEs impact horizontal gene transfer and the evolution of staphylococci.","PeriodicalId":89441,"journal":{"name":"Mobile genetic elements","volume":"7 4","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2017-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/2159256X.2017.1368433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35425085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Proteomics technique opens new frontiers in mobilome research. 蛋白质组学技术开辟了移动组研究的新领域。

Mobile genetic elements Pub Date : 2017-08-01 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1362494

Andrew D Davidson, David A Matthews, Kevin Maringer

{"title":"Proteomics technique opens new frontiers in mobilome research.","authors":"Andrew D Davidson, David A Matthews, Kevin Maringer","doi":"10.1080/2159256X.2017.1362494","DOIUrl":"10.1080/2159256X.2017.1362494","url":null,"abstract":"A large proportion of the genome of most eukaryotic organisms consists of highly repetitive mobile genetic elements. The sum of these elements is called the \"mobilome,\" which in eukaryotes is made up mostly of transposons. Transposable elements contribute to disease, evolution, and normal physiology by mediating genetic rearrangement, and through the \"domestication\" of transposon proteins for cellular functions. Although 'omics studies of mobilome genomes and transcriptomes are common, technical challenges have hampered high-throughput global proteomics analyses of transposons. In a recent paper, we overcame these technical hurdles using a technique called \"proteomics informed by transcriptomics\" (PIT), and thus published the first unbiased global mobilome-derived proteome for any organism (using cell lines derived from the mosquito Aedes aegypti). In this commentary, we describe our methods in more detail, and summarise our major findings. We also use new genome sequencing data to show that, in many cases, the specific genomic element expressing a given protein can be identified using PIT. This proteomic technique therefore represents an important technological advance that will open new avenues of research into the role that proteins derived from transposons and other repetitive and sequence diverse genetic elements, such as endogenous retroviruses, play in health and disease.","PeriodicalId":89441,"journal":{"name":"Mobile genetic elements","volume":"7 4","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8b/c5/kmge-07-04-1362494.PMC5599074.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35425084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transposable elements in Drosophila. 果蝇中的可转座元件

Mobile genetic elements Pub Date : 2017-04-19 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1318201

Tabitha J McCullers, Mindy Steiniger

引用次数: 0

Somatizing the transposons action. 将转座子的作用具体化。

Mobile genetic elements Pub Date : 2017-04-13 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1314236

Elgion L S Loreto, Camila Moura Pereira

引用次数: 9

Mobilizable genomic islands, different strategies for the dissemination of multidrug resistance and other adaptive traits. 可移动的基因组岛，传播多药耐药和其他适应性性状的不同策略。

Mobile genetic elements Pub Date : 2017-04-12 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1304193

Nicolas Carraro, Nicolas Rivard, Vincent Burrus, Daniela Ceccarelli

{"title":"Mobilizable genomic islands, different strategies for the dissemination of multidrug resistance and other adaptive traits.","authors":"Nicolas Carraro, Nicolas Rivard, Vincent Burrus, Daniela Ceccarelli","doi":"10.1080/2159256X.2017.1304193","DOIUrl":"https://doi.org/10.1080/2159256X.2017.1304193","url":null,"abstract":"Mobile genetic elements are near ubiquitous DNA segments that revealed a surprising variety of strategies for their propagation among prokaryotes and between eukaryotes. In bacteria, conjugative elements were shown to be key drivers of evolution and adaptation by efficiently disseminating genes involved in pathogenicity, symbiosis, metabolic pathways, and antibiotic resistance. Conjugative plasmids of the incompatibility groups A and C (A/C) are important vehicles for the dissemination of antibiotic resistance and the consequent global emergence and spread of multi-resistant pathogenic bacteria. Beyond their own mobility, A/C plasmids were also shown to drive the mobility of unrelated non-autonomous mobilizable genomic islands, which may also confer further advantageous traits. In this commentary, we summarize the current knowledge on different classes of A/C-dependent mobilizable genomic islands and we discuss other DNA hitchhikers and their implication in bacterial evolution. Furthermore, we glimpse at the complex genetic network linking autonomous and non-autonomous mobile genetic elements, and at the associated flow of genetic information between bacteria.","PeriodicalId":89441,"journal":{"name":"Mobile genetic elements","volume":"7 2","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/2159256X.2017.1304193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34938861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Can transposons be the obstacle to identical genetic cloning from somatic cells? 转座子会成为体细胞克隆相同基因的障碍吗?

Mobile genetic elements Pub Date : 2017-04-11 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1316889

Süleyman Aydın

引用次数: 0

Physical enrichment of transposon mutants from saturation mutant libraries using the TraDISort approach. 利用TraDISort方法对饱和突变文库中的转座子突变体进行物理富集。

Mobile genetic elements Pub Date : 2017-03-31 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1313805

Ian T Paulsen, Amy K Cain, Karl A Hassan

{"title":"Physical enrichment of transposon mutants from saturation mutant libraries using the TraDISort approach.","authors":"Ian T Paulsen, Amy K Cain, Karl A Hassan","doi":"10.1080/2159256X.2017.1313805","DOIUrl":"https://doi.org/10.1080/2159256X.2017.1313805","url":null,"abstract":"Transposon-insertion sequencing methods are finding their way into the molecular toolbox of many fields of microbiology. These methods can identify the genomic locations and density of transposon insertions in saturated transposon mutant libraries and can be used to make inferences on gene function. For example, where no insertions or very few insertions are identified within a gene in a mutant library grown under permissive conditions, the gene may be essential. Furthermore, where mutations are enriched or lost in a gene after passaging the library through a selective process, the gene is likely to be involved in phenotypes linked to the process. Typically, a fitness based selection such as a stress condition is used in these experiments and the processed sequencing data are used to identify genes required for fitness under the selection. Our research team recently expanded the utility of the transposon directed insertion sequencing (TraDIS) method by applying a physical separation of a transposon mutant library mediated by fluorescence activated cell sorting, rather than a fitness-based selection. This approach, which we have named \"TraDISort\" is significant because it allows the study of phenotypes that are not linked to cell survival. The TraDISort approach has a broad range of future applications, in drug development, metabolic engineering and in studies of basic bacterial cell physiology.","PeriodicalId":89441,"journal":{"name":"Mobile genetic elements","volume":"7 3","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/2159256X.2017.1313805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35060778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Population and clinical genetics of human transposable elements in the (post) genomic era. （后）基因组时代人类转座因子的种群和临床遗传学。

Mobile genetic elements Pub Date : 2017-01-11 eCollection Date: 2017-01-01 DOI: 10.1080/2159256X.2017.1280116

Lavanya Rishishwar, Lu Wang, Evan A Clayton, Leonardo Mariño-Ramírez, John F McDonald, I King Jordan

{"title":"Population and clinical genetics of human transposable elements in the (post) genomic era.","authors":"Lavanya Rishishwar, Lu Wang, Evan A Clayton, Leonardo Mariño-Ramírez, John F McDonald, I King Jordan","doi":"10.1080/2159256X.2017.1280116","DOIUrl":"10.1080/2159256X.2017.1280116","url":null,"abstract":"Recent technological developments-in genomics, bioinformatics and high-throughput experimental techniques-are providing opportunities to study ongoing human transposable element (TE) activity at an unprecedented level of detail. It is now possible to characterize genome-wide collections of TE insertion sites for multiple human individuals, within and between populations, and for a variety of tissue types. Comparison of TE insertion site profiles between individuals captures the germline activity of TEs and reveals insertion site variants that segregate as polymorphisms among human populations, whereas comparison among tissue types ascertains somatic TE activity that generates cellular heterogeneity. In this review, we provide an overview of these new technologies and explore their implications for population and clinical genetic studies of human TEs. We cover both recent published results on human TE insertion activity as well as the prospects for future TE studies related to human evolution and health.","PeriodicalId":89441,"journal":{"name":"Mobile genetic elements","volume":"7 1","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2017-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/06/db/kmge-07-01-1280116.PMC5305044.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34756906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0