Biofizika最新文献_第4页

[On the Role of Titin Phosphorylation in Development of Muscular Atrophy]. Titin磷酸化在肌萎缩发生中的作用

Biofizika Pub Date : 2015-07-01

N N Salmov, Yu V Gritsyna, A D Ulanova, I M Vikhlyantsev, Z A Podlubnaya

引用次数: 0

[Determination of a Spectrum of Lytic Activity of Bacteriophages by the Method of Acoustic Analysis]. [用声学分析方法测定噬菌体的裂解活性谱]。

Biofizika Pub Date : 2015-07-01

O I Guliy, B D Zaitsev, I E Kuznetsova, A M Shikhabudinov, L A Dykman, S A Staroverov, O A Karavaeva, S A Pavliy, O V Ignatov

{"title":"[Determination of a Spectrum of Lytic Activity of Bacteriophages by the Method of Acoustic Analysis].","authors":"O I Guliy, B D Zaitsev, I E Kuznetsova, A M Shikhabudinov, L A Dykman, S A Staroverov, O A Karavaeva, S A Pavliy, O V Ignatov","doi":"","DOIUrl":"","url":null,"abstract":"The changes in the electro-acoustic parameters of cell suspension due to the interaction of cells with bacteriophages both in a pure. culture and in the presence of extraneous microflora were investigated. It has been found that the specific changes in the electroacoustic parameters of cell suspension under the action of bacteriophage occur only in microbial cells which are sensitive to the bacteriophage studied. It has been established that a sensor unit allows of distinguishing a situation when the bacterial cells are infected with specific bacteriophages of the control experiments and a situation with no introduction of infection. An approximate criterion of the presence of specific interactions of bacteriophages and cells in suspension was developed. In accordance with this criterion the change in electrical impedance of the sensor unit must not be less than - 1%. In control experiments a standard microbiological technique, plating the cells infected with bacteriophages on solid nutrient medium, was used. For the first time the possibility of using the method of electroacoustic analysis for determination of a spectrum of lytic activity of bacteriophages was shown. The results obtained may be used for development of a new express method for determining the sensitivity to bacteriophages of the microbial cells.","PeriodicalId":8942,"journal":{"name":"Biofizika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34194579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Estimation of the Index Value of Dielectric Permeability inside the Membranes of Purple Bacteria]. [紫色细菌膜内介电通透性指标值的估算]。

Biofizika Pub Date : 2015-07-01

A Y Borisov, V S Kozlovsky

引用次数: 0

[Impact of Directed Migration on Formation of Spatial Structures of Populations]. [定向迁移对种群空间结构形成的影响]。

Biofizika Pub Date : 2015-07-01

A V Budyansky, V G Tsybulin

引用次数: 0

[Radioprotectors: History, Trends and Prospects]. [辐射防护:历史、趋势和前景]。

Biofizika Pub Date : 2015-07-01

S V Gudkov, N R Popova, V I Bruskov

引用次数: 0

[Potential-dependent Cation Selective Ion Channels Formed by Peroxiredoxin 6 in the Lipid Bilayer]. [过氧化氧还蛋白6在脂质双分子层形成的电位依赖性阳离子选择性离子通道]。

Biofizika Pub Date : 2015-07-01

P A Grigoriev, M G Sharapov, V I Novoselov

引用次数: 0

[Divergence of Chlorophyll Dynamics in the Naroch Lakes]. [Naroch湖泊叶绿素动态的差异]。

Biofizika Pub Date : 2015-07-01

B V Adamovich, R Z Kovalevskaya, N P Radchikova, T V Zhukova, T M Mikheyeva, A B Medvinsky, N I Nuriyeva, A V Rusakov

引用次数: 0

[Investigation of Inulinase Permolecular Organization from Producers of the Genus Aspergillus by Means of Some Computing and Experimental Methods]. 用一些计算和实验方法研究曲霉属植物菊粉酶的过分子组织

Biofizika Pub Date : 2015-07-01

M G Holyavka, V G Artyukhov, S M Makin

引用次数: 0

Biofizika Pub Date : 2015-07-01

E V Grishina, Ya V Khaustova, A A Vasilieva, E I Mayevsky

{"title":"[Age-related Peculiarities of Succinate Effect on Induced Lipid Peroxidation in Rat Liver Mitochondria].","authors":"E V Grishina, Ya V Khaustova, A A Vasilieva, E I Mayevsky","doi":"","DOIUrl":"","url":null,"abstract":"The antioxidant effect of succinate and 3-hydroxybutyrate oxidation on the kinetics of lipid peroxidation induced by ATP-Fe2+ complex in isolated rat liver mitochondria of old (1.0-1.5 years) and young (3 months) male rats was investigated. The rate of induced lipid peroxidation V(LPO) in rat liver mitochondria and the half-time of oxygen consumption Δt50, which included the lag period and the initiation. phase, was recorded polarographically. Without exogenous oxidative-substrates V(LPO) was slightly higher in mitochondria of old animals, but the onset of lipid peroxidation cascade was significantly earlier than in young animals. Incubation of mitochondria with 5mM succinate for 1 min inhibited V(LPO) by 15% in young animals and by 35% in old animals. However, only in mitochondria of old animals Δt50 increased by 19% as compared to lipid peroxidation without substrates. V(LPO) in mitochondria of young animals did not significantly change during 3-hydroxybutyrate oxidation, while in mitochondria of old animals it was reduced by 19% with a slight increase in Δt50. To simulate age-dependent dysfunction we damaged isolated mitochondria by a series of freeze-thaw cycles, which caused a significant increase of V(LPO) of.both age groups. Succinate oxidation inhibited V(LPO) in damaged mitochondria in all cases by 56%, as compared to V(LPO) without oxidative substrates and extended At50 twofold in mitochondria of young animals. Oxidation of 3-hydroxybutyrate had no effect on V(LPO) in damaged mitochondria regardless of animal, age and extended Δt50 by 48% in mitochondria of young animals. Thus, the antioxidant effect of succinate oxidation can prevent lipid peroxidation damage and may exhibit geroprotective action at the level of aging mitochondria. Therefore, the antioxidant effect is due to the process of substrate oxidation in the respiratory chain but not because of an interaction of their structures with membrane lipids per se.","PeriodicalId":8942,"journal":{"name":"Biofizika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34194578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification of Intermediate States of Electron Transfer Proteins Plastocyanin and Cytochrome f in the Process of Diffusional Encounter]. [扩散相遇过程中电子转移蛋白质体青素和细胞色素f中间态的鉴定]。

Biofizika Pub Date : 2015-07-01

S S Khruschev, A M Abaturova, V A Fedorov, I B Kovalenko, G Yu Riznichenko, A B Rubin

引用次数: 0