ISRN urology最新文献_第8页

AKT/mTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells. AKT/mTOR作为多酚皮杉醇的新靶点可能有助于抑制培养前列腺癌细胞的增殖。

ISRN urology Pub Date : 2012-01-01 Epub Date: 2012-04-03 DOI: 10.5402/2012/272697

Tze-Chen Hsieh, Chia-Yi Lin, Hung-Yun Lin, Joseph M Wu

{"title":"AKT/mTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells.","authors":"Tze-Chen Hsieh, Chia-Yi Lin, Hung-Yun Lin, Joseph M Wu","doi":"10.5402/2012/272697","DOIUrl":"https://doi.org/10.5402/2012/272697","url":null,"abstract":"The polyphenol piceatannol has shown inhibition against tyrosine and serine/threonine kinases. Whether piceatannol also exerts activity on the mammalian target of rapamycin (mTOR), a kinase involved in growth control of eukaryotic cells, is not known. In this study, we tested the effects of piceatannol on proliferation of androgen-dependent (AD) LNCaP and androgen-independent (AI) DU145 and PC-3 prostate cancer (CaP) cells. Suppression of AD and AI CaP cell growth by piceatannol was accompanied by cell cycle blockade in G(1)/S and S phases for LNCaP and PC-3 and induction of apoptosis in DU145 cells. Induction of apoptosis by piceatannol in DU145 cells was evident by reduced expression of poly(ADP-ribose) polymerase (PARP), cleavage of caspase 3 and apoptosis inducing factor AIF, and an increase in cytochrome c. The apoptotic changes occurred in concordance with DNA damage, supported by increased phosphorylated histone H2AX. Immunoblot analyses showed that exposure of different-stage CaP cells to piceatannol also resulted in cell-type-specific downregulation of mTOR and its upstream and downstream effector proteins, AKT and eIF-4E-BP1. We propose that the observed AKT and mTOR changes are new targets of piceatannol possibly contributing to its inhibitory activities on proliferation of CaP cells.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":"2012 ","pages":"272697"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/272697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30602003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Immunohistochemical evaluation of cell cycle regulators: impact on predicting prognosis in stage t1 urinary bladder cancer. 细胞周期调节因子的免疫组化评价:对预测t1期膀胱癌预后的影响。

ISRN urology Pub Date : 2012-01-01 Epub Date: 2012-12-06 DOI: 10.5402/2012/379081

Hans Olsson, Per Hultman, Nastaran Monsef, Johan Rosell, Staffan Jahnson

{"title":"Immunohistochemical evaluation of cell cycle regulators: impact on predicting prognosis in stage t1 urinary bladder cancer.","authors":"Hans Olsson, Per Hultman, Nastaran Monsef, Johan Rosell, Staffan Jahnson","doi":"10.5402/2012/379081","DOIUrl":"https://doi.org/10.5402/2012/379081","url":null,"abstract":"Background and Objective. The cell cycle is regulated by proteins at different checkpoints, and dysregulation of this cycle plays a role in carcinogenesis. Matrix metalloproteinases (MMPs) are enzymes that degrade collagen and promote tumour infiltration. The aim of this study was to evaluate the expression of various cell cycle regulators and MMPs and to correlate such expression with progression and recurrence in patients with stage T1 urothelial carcinoma of the bladder (UCB). Patients and Methods. This population-based cohort study comprised 201 well-characterized patients with primary stage T1 urothelial carcinoma of the bladder. Immunohistochemistry was performed on formalin-fixed material to quantify expression of cell cycle regulators and two MMPs. Results. Normal expression of p53 and abnormal expression of MMP9 were associated with greater risk of tumour recurrence. Also, normal p16 expression was related to a lower risk of tumour progression. MMP2, p21, cyclin D1, and pRb showed no significant results that could estimate progression or recurrence. Conclusions. Normal p16 expression is associated with a lower risk of tumour progression, but immunohistochemistry on cell cycle regulators and MMPs has little value in predicting the prognosis in stage T1 UCB.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":"2012 ","pages":"379081"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/379081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31151130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Morbidity and Quality of Life in Bladder Cancer Patients following Cystectomy and Urinary Diversion: A Single-Institution Comparison of Ileal Conduit versus Orthotopic Neobladder. 膀胱切除术和尿转移后膀胱癌患者的发病率和生活质量:回肠导管与原位新膀胱的单机构比较。

ISRN urology Pub Date : 2012-01-01 Epub Date: 2012-02-06 DOI: 10.5402/2012/342796

Barbara Erber, Mark Schrader, Kurt Miller, Martin Schostak, Daniel Baumunk, Anja Lingnau, Andres Jan Schrader, Florian Jentzmik

{"title":"Morbidity and Quality of Life in Bladder Cancer Patients following Cystectomy and Urinary Diversion: A Single-Institution Comparison of Ileal Conduit versus Orthotopic Neobladder.","authors":"Barbara Erber, Mark Schrader, Kurt Miller, Martin Schostak, Daniel Baumunk, Anja Lingnau, Andres Jan Schrader, Florian Jentzmik","doi":"10.5402/2012/342796","DOIUrl":"https://doi.org/10.5402/2012/342796","url":null,"abstract":"Objective. To evaluate and compare noncontinent and continent urinary diversion after radical cystectomy in patients with bladder cancer. Methods. A total of 301 patients submitted to radical cystectomy at the Charité-University Hospital Berlin from 1993 to 2007 including 146 with an ileal conduit and 115 with an ileal neobladder. Clinical and pathological data as well as oncological outcome were retrospectively analyzed and compared. Quality of life was analyzed using the EORTC QLQ-C30 and BLM30 questionnaires. Results. 69.1% and 69.6% of all patients who received an ileal conduit and ileal neobladder, respectively, developed early complications. The two groups differed significantly concerning the occurrence of postoperative ileus (P = 0.02) favoring patients who received an ileal conduit but not with regard to any other early-onset complication evaluated. Patients with ileal neobladder had a significantly better global health status and quality of life (P = 0.02), better physical functioning (P = 0.02), but also a higher rate of diarrhoea (P = 0.004). Conclusion. Cystectomy with any type of diversion remains a complication-prone surgery. Even if the patient groups are not homogeneous in all respects, there are many arguments in favor of the ileal neobladder as the urinary diversion of choice.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":" ","pages":"342796"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/342796","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40173550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 71

Antioncogenic effects of transient receptor potential vanilloid 1 in the progression of transitional urothelial cancer of human bladder. 瞬时受体电位香草样蛋白1在人膀胱移行性尿路上皮癌进展中的抗逆性作用。

ISRN urology Pub Date : 2012-01-01 Epub Date: 2012-02-06 DOI: 10.5402/2012/458238

Giorgio Santoni, Sara Caprodossi, Valerio Farfariello, Sonia Liberati, Angela Gismondi, Consuelo Amantini

{"title":"Antioncogenic effects of transient receptor potential vanilloid 1 in the progression of transitional urothelial cancer of human bladder.","authors":"Giorgio Santoni, Sara Caprodossi, Valerio Farfariello, Sonia Liberati, Angela Gismondi, Consuelo Amantini","doi":"10.5402/2012/458238","DOIUrl":"https://doi.org/10.5402/2012/458238","url":null,"abstract":"The progression of normal cells to a tumorigenic and metastatic state involves the accumulation of mutations in multiple key signaling proteins, encoded by oncogenes and tumor suppressor genes. Recently, members of the TRP channel family have been included in the oncogenic and tumor suppressor protein family. TRPM1, TRPM8, and TRPV6 are considered to be tumor suppressors and oncogenes in localized melanoma and prostate cancer, respectively. Herein, we focus our attention on the antioncogenic properties of TRPV1. Changes in TRPV1 expression occur during the development of transitional cell carcinoma (TCC) of human bladder. A progressive decrease in TRPV1 expression as the TCC stage increases triggers the development of a more aggressive gene phenotype and invasiveness. Finally, downregulation of TRPV1 represents a negative prognostic factor in TCC patients. The knowledge of the mechanism controlling TRPV1 expression might improve the diagnosis and new therapeutic strategies in bladder cancer.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":" ","pages":"458238"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/458238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40173551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Decreased vitamin b(12) levels in children with nocturnal enuresis. 夜间遗尿症患儿维生素b(12)水平降低。

ISRN urology Pub Date : 2012-01-01 Epub Date: 2012-01-26 DOI: 10.5402/2012/789706

Bülent Altunoluk, Mehmet Davutoglu, Mesut Garipardic, Vedat Bakan

{"title":"Decreased vitamin b(12) levels in children with nocturnal enuresis.","authors":"Bülent Altunoluk, Mehmet Davutoglu, Mesut Garipardic, Vedat Bakan","doi":"10.5402/2012/789706","DOIUrl":"https://doi.org/10.5402/2012/789706","url":null,"abstract":"Objectives. Nocturnal enuresis is a common pediatric problem, the etiology of which is unclear. In the present study, vitamin B(12) and folate levels were measured in children with nocturnal enuresis and compared with those in healthy control group children to investigate whether there was any relation with enuresis and neurogenic maturation as a first time in the literature. Methods. In this cross-sectional study, we included thirty children (16 girls, 14 boys) who had presented with primary nocturnal enuresis (PNE) complaints in the study group and 31 children (13 girls, 18 boys) in the control group. Body weight and height measurements were obtained and complete blood counts and vitamin B(12) and folate levels were measured in all children. Results. No difference was found in age, height, and weight between study and control groups. Also the mean levels of the hemoglobin, hematocrit, and mean corpuscular volume (MCV) were not different between the two groups. Significantly lower mean vitamin B(12) and folate levels were found in the enuresis group compared with the control group. Conclusions. Further studies are needed to clarify B(12) and folate deficiency in larger series so that these tests can be included in routine investigations of enuretic children.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":" ","pages":"789706"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/789706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40173552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Endourological Management of Urolithiasis in Donor Kidneys prior to Renal Transplant. 肾移植前供肾尿石症的腔内管理。

ISRN urology Pub Date : 2011-01-01 Epub Date: 2011-06-22 DOI: 10.5402/2011/242690

Nikhil Vasdev, John Moir, Muhammed T Dosani, Robert Williams, Naeem Soomro, David Talbot, David Rix

{"title":"Endourological Management of Urolithiasis in Donor Kidneys prior to Renal Transplant.","authors":"Nikhil Vasdev, John Moir, Muhammed T Dosani, Robert Williams, Naeem Soomro, David Talbot, David Rix","doi":"10.5402/2011/242690","DOIUrl":"https://doi.org/10.5402/2011/242690","url":null,"abstract":"Background. We present our centres successful endourological methodology of ex vivo ureteroscopy (EVFUS) in the management of these kidneys prior to renal transplantation. Patient and Methods. A retrospective analysis was performed of all living donors (n = 157) identified to have asymptomatic incidental renal calculi from January 2004 until December 2008. The incidence of asymptomatic renal calculi was 3.2% (n = 5). Donors were subdivided into 2 groups depending on whether theydonated the kidney with the renal calculus (Group 1) versus the opposite calculus-free kidney (Group 2). Results. All donors in Group 1 underwent a left laparoscopic donor nephrectomy. The calculi were extracted in all 3 cases using a 7.5 Fr flexible ureteroscope either prior to transplant (n = 2) or on revascularization (n = 1). There were no urological complications in either group. At a mean followup at 64 months there was no recurrent calculi formation in the recipient in Group 1. However, 1 recipient formed a calculus in group 2 at a follow up of 72 months. Conclusions. Renal calculi can be successfully retrieved during living-related transplantation at the time of transplant itself using EVUS. This is technically feasible and is associated with no compromise in ureteral integrity or renal allograft function.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":"2011 ","pages":"242690"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2011/242690","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30254368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Intravesical tension-free vaginal tape removal: is there a single solution? 阴道内无张力阴道胶带拔除:有单一的解决方案吗?

ISRN urology Pub Date : 2011-01-01 Epub Date: 2011-04-03 DOI: 10.5402/2011/343850

Rhana H Zakri, Amit K Patel, Babbin S John, Nitin C Shrotri

引用次数: 1

Mule bite to the male genitalia with complete penile and anterior urethra amputation: unusual case and review of the literature. 骡子咬伤男性生殖器并导致阴茎和前尿道完全截断：罕见病例和文献综述。

ISRN urology Pub Date : 2011-01-01 Epub Date: 2011-04-19 DOI: 10.5402/2011/723154

M A Lakmichi, B Wakrim, R Jarir, Z Dahami, M S Moudouni, I Sarf

引用次数: 0

A novel antireflux technique for orthotopic ileal bladder substitutes-flat-segment technique: preliminary results. 一种新的原位回肠膀胱替代物抗反流技术——平节段技术:初步结果。

ISRN urology Pub Date : 2011-01-01 Epub Date: 2011-09-14 DOI: 10.5402/2011/431951

Hany Elfayoumy, Ashraf Abou-Elela, Tamer Orban, Ashraf Emran, Mohamed Elghoneimy, Ahmed Morsy

{"title":"A novel antireflux technique for orthotopic ileal bladder substitutes-flat-segment technique: preliminary results.","authors":"Hany Elfayoumy, Ashraf Abou-Elela, Tamer Orban, Ashraf Emran, Mohamed Elghoneimy, Ahmed Morsy","doi":"10.5402/2011/431951","DOIUrl":"10.5402/2011/431951","url":null,"abstract":"Objective. Although a large debate exists regarding the need for reflux prevention in ileal orthotopic neobladders, it is our policy to continue performing nonrefluxing ureteroileal anastomoses for our patients. An ideal uretero-ileal anastomosis must be simple, nonrefluxing, as well as non-obstructive. Here, we present a new antireflux mechanism for orthotopic ileal neobladders. Methods. 12 radical cystectomy patients for muscle invasive bladder cancer were candidates for orthotopic urinary diversion and underwent a non-refluxing uretero-ileal anastomosis using the flat-segment technique with a follow up of 6 to 18 months. Results. Preliminary results after the short-term followup showed that the success rate in reflux prevention was 92% and no cases of obstruction. The upper tracts were preserved or improved in all 12 patients. Operative time for neobladder creation ranged between 120-240 minutes, with a mean of 165 minutes (±36 minutes). No diversion-related complications. Conclusions. Based on our early data, we believe that the flat-segment uretero-ileal anastomosis technique for reflux prevention in orthotopic ileal bladder substitutes is simple, easy to learn and carries no additional morbidity to a standard refluxing uretero-ileal anastomosis, but has the advantage of effective reflux prevention. A longer follow-up period study with more patient numbers is ongoing.","PeriodicalId":89401,"journal":{"name":"ISRN urology","volume":"2011 ","pages":"431951"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30378118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Metastatic bladder cancer: a review of current management. 转移性膀胱癌:目前治疗的回顾。

ISRN urology Pub Date : 2011-01-01 Epub Date: 2011-06-09 DOI: 10.5402/2011/545241

Andrew Fletcher, Ananya Choudhury, Nooreen Alam

引用次数: 26