The open parasitology journal最新文献_第5页

Trypanosoma cruzi Peptidases: An Overview 克氏锥虫肽酶:综述

The open parasitology journal Pub Date : 2010-03-15 DOI: 10.2174/1874421401004010120

Alane Beatriz Vermelho

引用次数: 6

Negative Staining Technique of Heine for the Detection of Cryptosporidium spp.: A Fast and Simple Screening Technique~!2009-11-13~!2009-01-06~!2010-02-11~! 海涅阴性染色法检测隐孢子虫:一种快速简便的筛选技术

The open parasitology journal Pub Date : 2010-02-11 DOI: 10.2174/1874421401004010001

I. Potters, M. Esbroeck

引用次数: 19

Sand fly-Leishmania interactions: long relationships are not necessarily easy. 沙蝇与利什曼原虫的相互作用:长期的关系并不一定容易。

The open parasitology journal Pub Date : 2010-01-01 DOI: 10.2174/1874421401004010195

Marcelo Ramalho-Ortigao, Elvira M Saraiva, Yara M Traub-Csekö

{"title":"Sand fly-Leishmania interactions: long relationships are not necessarily easy.","authors":"Marcelo Ramalho-Ortigao, Elvira M Saraiva, Yara M Traub-Csekö","doi":"10.2174/1874421401004010195","DOIUrl":"https://doi.org/10.2174/1874421401004010195","url":null,"abstract":"Sand fly and Leishmania are one of the best studied vector-parasite models. Much is known about the development of these parasites within the sand fly, and how transmission to a suitable vertebrate host takes place. Various molecules secreted by the vector assist the establishment of the infection in a vertebrate, and changes to the vector are promoted by the parasites in order to facilitate or enhance transmission. Despite a generally accepted view that sand flies and Leishmania are also one of the oldest vector-pathogen pairs known, such long history has not been translated into a harmonic relationship. Leishmania are faced with many barriers to the establishment of a successful infection within the sand fly vector, and specific associations have been developed which are thought to represent aspects of a co-evolution between the parasite and its vectors. In this review, we highlight the journey taken by Leishmania during its development within the vector, and describe the issues associated with the natural barriers encountered by the parasite. Recent data revealed sexual replication of Leishmania within the sand fly, but it is yet unknown if such reproduction affects disease outcome. New approaches targeting sand fly molecules to prevent parasite transmission are being sought, and various techniques related to genetic manipulation of sand flies are being utilized.","PeriodicalId":89294,"journal":{"name":"The open parasitology journal","volume":" ","pages":"195-204"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805382/pdf/nihms394690.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40264792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

REGULATION of the EXTRACELLULAR MATRIX INTERACTOME by Trypanosoma cruzi. 克氏锥虫对细胞外基质相互作用的调控。

The open parasitology journal Pub Date : 2010-01-01 DOI: 10.2174/1874421401004010072

Tatiana C Cardenas, Candice A Johnson, Siddharth Pratap, Pius N Nde, Vyacheslav Furtak, Yuliya Y Kleshchenko, Maria F Lima, Fernando Villalta

{"title":"REGULATION of the EXTRACELLULAR MATRIX INTERACTOME by Trypanosoma cruzi.","authors":"Tatiana C Cardenas, Candice A Johnson, Siddharth Pratap, Pius N Nde, Vyacheslav Furtak, Yuliya Y Kleshchenko, Maria F Lima, Fernando Villalta","doi":"10.2174/1874421401004010072","DOIUrl":"https://doi.org/10.2174/1874421401004010072","url":null,"abstract":"It has been shown that the invasive trypomastigote forms of Trypanosoma cruzi use and modulate components of the extracellular matrix (ECM) during the initial process of infection. Infective trypomastigotes up-regulate the expression of laminin γ-1 (LAMC1) and thrombospondin (THBS1) to facilitate the recruitment of trypomastigotes to enhance cellular infection. Silencing the expression of LAMC1 and THBS1 by stable RNAi dramatically reduces trypanosome infection. T. cruzi gp83, a ligand that mediates the attachment of trypanosomes to cells to initiate infection, up-regulates LAMC1 expression to enhance cellular infection. Infective trypomastigotes interact with LAMC1 through galectin-3 (LGALS3), a human lectin, to enhance cellular infection. Silencing the expression of LGALS3 also reduces cellular infection. Some trypanosome surface molecules also interact with the ECM to facilitate infection. Despite the role of the ECM in T. cruzi infection, almost nothing is known about the ECM interactome networks operating in the process of T. cruzi infection. In this mini review, we critically analyze and discuss the regulation of the ECM by T. cruzi and its gp83 ligand, and present the first elucidation of the human ECM interactome network, regulated by T. cruzi and its gp83 ligand, to facilitate cellular infection. The elucidation of the human ECM interactome regulated by T. cruzi is critically important to the understanding of the molecular pathogenesis of T. cruzi infection and developing novel approaches of intervention in Chagas' disease.","PeriodicalId":89294,"journal":{"name":"The open parasitology journal","volume":"4 ","pages":"72-76"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075921/pdf/nihms260989.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29821521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Biomarkers of Attenuation in the Leishmania donovani Centrin Gene Deleted Cell Line—Requirements for Safety in a Live Vaccine Candidate 多诺瓦利什曼中心蛋白基因缺失细胞系衰减的生物标志物——候选活疫苗的安全性要求

The open parasitology journal Pub Date : 2009-12-02 DOI: 10.2174/1874421400903010009

R. Duncan, R. Deya, Keiko Tomiokaa, Heather Hairston, A. Selvapandiyan, H. Nakhasi

{"title":"Biomarkers of Attenuation in the Leishmania donovani Centrin Gene Deleted Cell Line—Requirements for Safety in a Live Vaccine Candidate","authors":"R. Duncan, R. Deya, Keiko Tomiokaa, Heather Hairston, A. Selvapandiyan, H. Nakhasi","doi":"10.2174/1874421400903010009","DOIUrl":"https://doi.org/10.2174/1874421400903010009","url":null,"abstract":"Biomarkers of the attenuated phenotype are needed to develop live attenuated parasites into safe Leishmania vaccines. The centrin-1 gene deleted strain of Leishmania donovani (LdCEN1 -/- ) shows promise as a vaccine candidate. To identify genes whose expression patterns are indicators of attenuation, the LdCEN1 -/- line was compared to wild type by gene expression microarray. Two genes, one coding for a 27kDa protein (p27) and another coding for putative Argini- nosuccinate Synthase (AS) have such expression patterns. Both genes express a higher RNA level in the amastigote stage than in the promastigote stage of wild type cells; however they are down-regulated in the LdCEN1 -/- amastigote cells. Western blots indicated that the AS protein level is also reduced in the LdCEN1 -/- amastigotes, while the p27 protein level is not reduced even when its mRNA level has diminished. Northern and Western blot analysis with these two biomarkers showed that LdCEN1 -/- parasites recovered after five weeks of infection in mice had the same expression pattern as they had prior to infection and episomal expression of centrin in the LdCEN1 -/- cells restored normal expression of both genes. Measurement of the expression of these two genes in infected macrophages by RT-PCR indicated the same pattern as in cultured cells. Therefore, both the mRNA and/or the protein levels of these two genes could be used as biomarkers of at- tenuation to monitor the safety of the LdCEN1 -/- cell line as it is developed as a potential vaccine.","PeriodicalId":89294,"journal":{"name":"The open parasitology journal","volume":"3 1","pages":"14-23"},"PeriodicalIF":0.0,"publicationDate":"2009-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85122341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Redescription of Monticellius indicum Mehra, 1939 (Digenea: Spirorchiidae)from the Heart of Green Sea Turtles (Chelonia mydas) in Costa Rica 哥斯达黎加绿海龟(Chelonia mydas)群中Monticellius indicum Mehra, 1939 (Digenea:螺旋藻科)的再描述

The open parasitology journal Pub Date : 2009-04-07 DOI: 10.2174/1874421400903010004

M. Santoro, E. Greiner, J. Morales, B. Rodríguez-Ortiz

引用次数: 5

Toxoplasmosis in Sheep: A Potential Risk of Infection Among Residents and Farm Workers in Lajes, Brazil 绵羊弓形虫病:巴西拉日斯居民和农场工人感染的潜在风险

The open parasitology journal Pub Date : 2009-01-22 DOI: 10.2174/1874421400903010001

M. Clementino, I. Barbosa, V. Andrade-Neto

引用次数: 3

NcGRA2-RT-PCR to Detect Live Versus Dead Parasites in Neospora caninum-Infected Mice NcGRA2-RT-PCR检测犬新孢子虫感染小鼠的活与死寄生虫

The open parasitology journal Pub Date : 2008-10-16 DOI: 10.2174/1874421400802010064

M. Strohbusch, N. Müller, A. Hemphill, G. Greif, B. Gottstein

{"title":"NcGRA2-RT-PCR to Detect Live Versus Dead Parasites in Neospora caninum-Infected Mice","authors":"M. Strohbusch, N. Müller, A. Hemphill, G. Greif, B. Gottstein","doi":"10.2174/1874421400802010064","DOIUrl":"https://doi.org/10.2174/1874421400802010064","url":null,"abstract":"In the present work, we optimized a recently established NcGRA2-RT-PCR based on RNA to detect live Neo- spora caninum parasites in tissue, and compared the results with the conventional inoculation of diagnostic specimen onto cell culture. C57BL/6 mice were experimentally infected with Nc-1 tachyzoites and subsequently euthanized 6 or 12 days post infection (dpi). Selected organs were used to search for parasites by (i) PCR using genomic DNA (gDNA), (ii) PCR using cDNA and (iii) in vitro inoculation of cell culture. At 6 dpi, Neospora-gDNA was detected in 34 out of 36 organs. Viable parasites were detected in 11 (NcGRA2-RT-PCR) and 15 (in vitro cultivation) out of 36 organs. Comparison of NcGRA2-RT-PCR and in vitro detection gave a fair agreement (kappa 0.29), whereas comparison of PCR using gDNA and RT-PCR or in vitro detection resulted in a slight agreement (kappa 0.05 and 0.08, respectively) only. At 12 dpi, para- site gDNA was found in 10 out of 36 organs. In 7 of these organs viability of parasites was confirmed with NcGRA2-RT- PCR and growth of parasites in cell culture. Comparison of NcGRA2-RT-PCR and in vitro detection gave a substantial agreement (kappa 0.8), whereas comparison of PCR using gDNA and RT-PCR or in vitro detection resulted in a moderate agreement (kappa 0.59 and 0.77, respectively). As NcGRA2-RT-PCR is almost as sensitive as in vitro cultivation in de- tecting live parasites, it represents a fast, easy and safe method of viable parasite detection, and thus an attractive alterna- tive to the in vitro cultivation approach.","PeriodicalId":89294,"journal":{"name":"The open parasitology journal","volume":"126 1","pages":"64-68"},"PeriodicalIF":0.0,"publicationDate":"2008-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79533967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Spatial Delimitation, Forecasting and Control of Japanese Encephalitis: India – A Case Study 空间划分，预测和控制日本脑炎:印度-个案研究

The open parasitology journal Pub Date : 2008-07-16 DOI: 10.2174/1874421400802010059

S. Sabesan, Hari Kishan Raju Konuganti, V. Perumal

引用次数: 17

Desynchronizing Plasmodium Cell Cycle Increases Chloroquine Protection at Suboptimal Doses 不同步的疟原虫细胞周期在次优剂量下增加氯喹的保护作用

The open parasitology journal Pub Date : 2008-06-30 DOI: 10.2174/1874421400802010055

P. Bagnaresi, R. Markus, C. T. Hotta, T. Pozzan, Celia R. S. Garcia

引用次数: 14