Biologia Plantarum最新文献

{"title":"Morphological diversity of seeds of Polish Festulolium cultivars depending on weather conditions","authors":"B. Borawska-Jarmułowicz, G. Mastalerczuk, E. Małuszyńska, W. Borucki","doi":"10.32615/bp.2020.146","DOIUrl":"https://doi.org/10.32615/bp.2020.146","url":null,"abstract":"Morphological traits of seeds of Festulolium braunii (K. Richt.) A. Camus cultivars were studied in relation to weather conditions. The obtained results show that morphological traits of seeds varied among cultivars and years of cultivation. The values of seed characteristics were higher in year 2016 due to more favorable weather conditions for plant growth. It was found that the husked seeds of Festulolium cultivars were characterized by a similar length and width regardless of the year, whereas naked seeds significantly differed in these characteristics. Mean values (from the research years) of seed parameters, such as thousand seed mass, area, convex area, shape factor, rectangle, as well as naked seed width showed variations among cultivars. At the same time, it was found that the mass of 1 000 seeds of individual cultivars showed the smallest variability in both years of the study (2.56 4.86 %) indicating a high stability of this trait. Thousand seed mass was significantly correlated with husked seed length / husked seed width ratio and husked seed length, and weakly correlated with yield. Additional key words: biometric parameters, husked and naked seeds, thousand seed mass. Submitted 16 June 2020, last revision 5 October 2020, accepted 6 October 2020. Abbreviations: A area of a particle; CA convex area; CP convex perimeter; D diameter; ECD equivalent circle diameter; F feret; HSL husked seed length; HSW husked seed width; MR Martins radius; NSL naked seed length; NSW naked seed width; P perimeter; R rectangle; RL rachilla length; SF shape factor; TSM thousand seed mass. Acknowledgements: The authors would like to thank Eugeniusz Paszkowski from the Plant Breeding Station in Szelejewo, Poland, for the provision of seed material. * Corresponding authors; e-mails: barbara_borawska_jarmulowicz@sggw.edu.pl, grazyna_mastalerczuk@sggw.edu.pl © The authors. This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"814-820"},"PeriodicalIF":1.5,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42246386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Festulolium field performance under fluctuating growing conditions in Lithuania","authors":"V. Kemešytė, K. Jaškūnė, G. Statkevičiūtė","doi":"10.32615/bp.2020.165","DOIUrl":"https://doi.org/10.32615/bp.2020.165","url":null,"abstract":"Festulolium cultivars are widely utilized in Lithuania because they are persistent under abiotic stresses and are high yielding. However, changing climate challenges the existing Festulolium cultivars to adapt to new growing conditions and still maintain the yield. In this study, we aimed at evaluating the yield stability of two Festulolium cultivars in field trials under fluctuating Lithuanian conditions. The mean total dry matter yield (DMY) of both Festulolium cultivars fluctuated greatly between the years and ANOVA analysis showed a significant effect of environment on total DMY as well as DMY of each cut, but the genotype × environment interaction was not significant. There was a high difference between the total DMY of 1st year and 2nd year of use of plots in each year of observation. The highest DMYs were harvested in the years 2015 and 2016. Dry matter yield of the 1st cut was the largest component of the total DMY for most of the years. The plants overwintered the first winter after sowing very well over the whole study period, resulting in excellent spring growth. The winter survival scores of 2nd year of use of plots were lower than 1st year of use and strongly correlated with the 1st cut DMY of 2nd year of use (r = 0.81). Spring growth of plants at 2nd year of use was poorer, the correlation between winter survival and spring growth of 2nd year of use was 0.62. The scores of regrowth after the cuts of 1st and 2nd years of use were very similar for most of the experimental years and moderately correlated with the sum of DMYs after cuts (r = 0.55 and r = 0.5, respectively). Additional key words: crown rust, dry matter yield, intergeneric hybrids, leaf spot diseases, winter survival. Submitted 18 September 2020, last revision 11 November 2020, accepted 13 November 2020. Abbreviations: DMY1 dry matter yield of the first cut; DMY2 dry matter yield of the second cut; DMY3 dry matter yield of the third cut; DMY4 dry matter yield of the fourth cut; RG regrowth after cuts; SG spring growth; WS winter survival; Y1 first year of use; Y2 second year of use. Acknowledgements: The study was carried out in the frame of the long-term research program “Genetic determination of the traits of agricultural and forest plants, development of modern cultivars” and the “Genome-wide functional analysis of perennial ryegrass for improved growth under water liming conditions (GrowGene)” project funded by the Research Council of Lithuania, Grant No. S-MIP-17-24. * Corresponding author; e-mail: grazina.statkeviciute@lammc.lt © The authors. This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43752550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia","authors":"L. Sang, L. Peng, Z. Qiu, F. Luo, G. Chen, L. Gao, Y. Yu, J. Liu","doi":"10.32615/bp.2020.151","DOIUrl":"https://doi.org/10.32615/bp.2020.151","url":null,"abstract":"Ferredoxin-thioredoxin reductase (FTR) is an iron-sulfur protein that supplies electrons from photochemically reduced ferredoxin (Fd) to thioredoxin (Trx) in the ferredoxin/thioredoxin system in chloroplasts. The FTR is a heterodimer with a variable subunit (FTRv) and a catalytic subunit (FTRc). The function of FTRv is not well known. In petunia (Petunia hybrida), FTRv is a single-copy gene, which is named PhFTRv. In this study, the spatio-temporal expression of PhFTRv in petunia was analyzed, and PhFTRv transcription was found to be high in leaves and stems. A tobacco rattle virus gene silencing was used in this study. Virus induced gene silencing-mediated PhFTRv silencing resulted in large yellow-green leaves, delayed flowering, and reduced cold tolerance in petunia plants. Additional key words: flowering, chloroplasts, chlorophyll, leaf development, Petunia hybrida, photosynthesis, temperature stress. Submitted 28 June 2020, last revision 27 September 2020, accepted 12 October 2020. Abbreviations: ci internal CO2 concentration; Fd ferredoxin; FTR ferredoxin-thioredoxin reductase; gs stomatal conductance; NTRC NADPH-dependent thioredoxin reductase C; PN net photosynthetic rate; TRV tobacco rattle virus; Trx thioredoxin; VIGS virus induced gene silencing. Acknowledgements: This research was supported by the National Natural Science Foundation of China (31770737, 31870692 and 31661143047) and the National Key Research and Development Plan (2018YFD100015). * Corresponding author; e-mail: juanxuliu@scau.edu.cn This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43337377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi","authors":"X. Zuo, F. Wang, X.R. Li, M.-M. Li","doi":"10.32615/bp.2020.154","DOIUrl":"https://doi.org/10.32615/bp.2020.154","url":null,"abstract":"Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression. However, the reference genes that can be used for qPCR of Rehmannia chingii and R. henryi have not yet been identified. In this study, based on the transcriptome data of R. chingii and R. henryi, we initially selected genes with relatively stable expression in different samples. We screened six candidate reference genes in R. chingii and R. henryi and calculated their expression abundance by real time qPCR. Their expression stability was evaluated by three algorithms geNorm, NormFinder, and BestKeeper. Although the results obtained by different algorithms were not completely consistent, R. chingii type 2A phosphatase activator TIP41 and R. chingii 18S ribosomal RNA had the highest expression stability in six different samples of R. chingii, and R. henryi 18S ribosomal RNA and R. henryi actin showed the most stable expression in different samples of R. henryi. In addition, based on transcriptome data, four genes were screened in R. chingii and R. henryi, and the expression stability of the selected reference genes was further verified. This study laid the foundation for further analysis and verification of the functions of important genes in R. chingii and R. henryi. Additional key words: BestKeeper, gene expression stability, geNorm, NormFinder. Submitted 13 July 2020, last revision 18 October 2020, accepted 19 October 2020. Abbreviations: 4CL 4-Coumarate-CoA ligase; C4H cinnamic acid 4-hydroxylase; Cq quantitative cycle; CV coefficient of variation; EF-1α elongation factor 1-alpha; ERF ethylene-responsive transcription factor; FPKM fragments per kilobase of transcript per million fragments mapped; GAPDH glyceraldehyde 3-phosphate dehydrogenase; M value gene expression stability measure; ORF open reading frame; PAL phenylalanine ammonia-lyase; 18S 18S ribosomal RNA; Tm melting temperature; UBQ ubiquitin. Acknowledgements. This research was supported by the National Natural Science Foundation of China (Grant No. 81872950) and the National Key Research and Development Program (2017YFC1700705). * Corresponding author; e-mail: heauzycxw@126.com This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"798-806"},"PeriodicalIF":1.5,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46558052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breeding and genetics of two new amphiploid Festulolium synthetics with improved yield and digestibility","authors":"J. Baert, K. Laere, C. Waes, A. Ghesquiere, J. Aper","doi":"10.32615/bp.2020.138","DOIUrl":"https://doi.org/10.32615/bp.2020.138","url":null,"abstract":"In order to introduce drought tolerance and improved cell wall digestibility from fescue in fodder ryegrasses, we developed two amphiploid Festulolium synthetics. One is a synthetic composed of three selected drought tolerant F1 hybrid genotypes from a cross between tetraploid Lolium multiflorum and hexaploid Festuca arundinacea, further on called LMFA. The other is a synthetic composed of five selected genotypes with soft leaves from a cross between tetraploid Lolium perenne and tetraploid Festuca pratensis, further on called LPFP. We produced seeds in polycrosses of two generations of both amphiploids, i.e., syn1 and syn2, and tested them in plot trials to determine the yield and fodder quality. The syn1 of both Festulolium populations had a higher annual dry matter yield than the reference Lolium cultivars and Festulolium cultivars composed of the same parental species. However, the syn2 of LMFA did not show an improved drought tolerance during a dry growing season compared to other Festulolium cultivars, and the seed yield of LMFA syn1 was low and dropped extremely in syn2. The number of chromosomes of LMFA also decreased gradually from F1 to syn2, and there was a clear shift in chromosome composition towards the Lolium genome. The LPFP synthetic performed better. Although the sugar content was significantly lower than the sugar content of the perennial ryegrass cultivars, organic matter digestibility (OMD) of LPFP was as high as OMD of the tetraploid perennial ryegrass cultivars. The cell wall digestibility (NDFD) of LPFP was significantly higher than the NDFD of both parental species and higher than the NDFD of all tested Festulolium cultivars. The seed yield of LPFP was the same in syn1 and syn2. The chromosome number remained on average the same and no clear shift of the chromosome composition to one of the composing genomes was observed. Overall, chromosome analysis revealed a high number of aneuploidy in syn1 and syn2 generations of both LMFA and LPFP and a lot of variation in number of Lolium, Festuca and recombinant chromosomes, and in the Lolium:Festuca genome ratio was observed among different genotypes of the same population. Therefore, selection for genotypes with a more stable genome composition will be a prerequisite for a sufficient seed yield and a broader exploitation of these new Festulolium synthetics. Additional key words: Festuca arundinacea, genomic in situ hybridization, forage yield, Lolium multiflorum, seed yield, GISH.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"789-797"},"PeriodicalIF":1.5,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47052521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cadmium and lead on the growth and the activity of peroxidase and superoxide dismutase of blueberry plantlets in vitro","authors":"J. Z. Chen, Hong Wang, C. Tian, Fan Yang, G. Z. Wang","doi":"10.32615/bp.2020.143","DOIUrl":"https://doi.org/10.32615/bp.2020.143","url":null,"abstract":"To study the effects of cadmium and lead on the growth of blueberry (Vaccinium ashei Reade) plantlets in vitro and on the activity of peroxidase (POD) and superoxide dismutase (SOD) of blueberry shoots, Cd2+ and Pb2+ were added separately to a cultivation medium. The results show that 0.01 mM Cd2+ significantly inhibited the growth of blueberry shoots, and the height and fresh mass of the shoots were significantly lower than those of the control; 0.05 mM Cd2+ significantly inhibited the proliferation of the shoots; the inhibitory effect on the growth and proliferation of blueberry in vitro was enhanced with the increase of Cd2+ concentration. Also Pb2+ (0.10 1.00 mM) significantly decreased the proliferation of the shoots, but it did not decrease significantly the shoot height and fresh mass. After 20 d of Cd or Pb treatments, the POD and SOD activities of the shoots increased with the increase of their concentrations, and when the concentration was 1.00 mM, the POD and SOD activities were significantly higher than in the control; the effect of Pb2+ on POD and SOD activities was generally stronger than that of Cd2+. The upregulation of activities of antioxidative enzymes played an effective role in acclimatization to these stresses, especially to Pb stress. Additional key words: antioxidants, heavy metals, Vaccinium ashei. Submitted 26 May 2020, last revision 20 September 2020, accepted 2 October 2020. Abbreviations: POD peroxidase; SOD superoxide dismutase. Acknowledgments: This work was financially supported by the National Natural Science Foundation of China (No. 20977027) and the Zhejiang Provincial Natural Science Foundation of China (No. LY16C020003). * Corresponding author; e-mail: jzchen@zjhu.edu.cn This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence In recent years, due to the impact of human activities, a large number of heavy metals such as cadmium and lead have been released into the soil, resulting in the increasingly serious pollution in the soil (Chen et al. 2014). Cd and Pb in soil not only affect the yield and quality of plants, but also accumulate in animals and human bodies through the food chain, threatening their health (Xiao et al. 2015, Tóth et al. 2016). Therefore, the content of Cd and Pb in contaminated soil and the tolerance mechanisms of plants to Cd and Pb stress attract great attention. Blueberries (Vaccinium spp.) are perennial berry fruit trees of Ericaceae family. Blueberry fruits are popular around the world due to their nutritional value and elevated content of bioactive phenolic compounds (Kalt et al. 2007, 2020). Although many species of blueberries are native to North America, several of them especially highbush (V. corymbosum L.), lowbush (V. angustifolium Ait.), and rabbiteye (V. ashei Reade) blueberries are commercially cultivated in many countries in Europe, South America, Asia, Australia, and New Zealand (Strik 2005, Strik and Yarborough 2005). Soil is one ","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"784-788"},"PeriodicalIF":1.5,"publicationDate":"2020-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47880241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet-B radiation induces cell death in root tips and reprograms metabolism in Arabidopsis","authors":"Peng Zhang, R. Wang, Ying Wang, J. Xu","doi":"10.32615/bp.2020.122","DOIUrl":"https://doi.org/10.32615/bp.2020.122","url":null,"abstract":"Ultraviolet-B (UV-B) radiation inhibits root system growth, however, the influence of UV-B radiation on the regulation of root development remains unclear. Here, we investigated the effects of UV-B radiation on root growth and metabolism in Arabidopsis using physiological, pharmacological, and transcriptome approaches. Our previous study has demonstrated that UV-B radiation depresses auxin accumulation in roots by reducing auxin biosynthesis, transport, and response. In this study, we found that UV-B radiation inhibited primary root (PR) growth by inducing root tip cell death and thereby disrupting cell division and elongation in root tips. The destructed root structure and distorted auxin flow caused by UV-B-induced root tip cell death also led to a reduced auxin accumulation in roots. Supplementation with an auxin α-naphthylacetic acid alleviated UV-B-repressed PR growth and further supported a notion that auxin is involved in UV-Brepressed PR growth. The UV-B radiation downregulated the expression of genes encoding the enzymes or regulators of the biosyntheses and degradations of the structural constituents of cell wall and genes involved in wax, cutin, and suberin biosyntheses, thereby repressing root system growth and development. The UV-B radiation also markedly repressed photosynthesis-related gene expression in roots, a non-photosynthetic organ. Taken together, this study suggests that UV-B radiation affects root growth by inducing cell death in root tips and reprogramming metabolism in roots. Additional key words: endogenous auxin flow, gene expression, α-naphthylacetic acid application, primary root growth. Submitted 2 April 2020, last revision 27 July 2020, accepted 19 August 2020. Abbreviations: ANAC032 NAC domain containing protein 32; bZIP60 basic region/leucine zipper motif 60; Col-0 Columbia-0; CYCs cell cycle regulators cyclins; DAPI 4,6-diamidino-2-phenylindole; DEGs differentially expressed genes; DIN2 dark inducible 2; GO gene ontology; IBS1 BABA-induced sterility 1; MYB2 myeloblastosis domain protein 2; NAA α-naphthylacetic acid; PCD programmed cell death; PI propidium iodide; PR primary root; ROS reactive oxygen species; SRA Short Read Archive; UV-B ultraviolet-B; WOX WUSCHEL-related homeobox. Acknowledgements: The authors gratefully acknowledge the Central Laboratory of the Xishuangbanna Tropical Botanical Garden for providing research facilities. This work was supported by the National Key Research and Development Program of China (2016YFC0501901), the China National Natural Sciences Foundation (31772383), the Basic Research Program of Qinghai Province (2019-ZJ-7033), and the Qinghai innovation platform construction project: Qinghai Provincial Key Laboratory of Restoration Ecology of Cold Area (2017-ZJ-Y20). * Corresponding authors; e-mails: zhangping2@xtbg.ac.cn; wangyb02@126.com This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"764-772"},"PeriodicalIF":1.5,"publicationDate":"2020-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46162310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying citrus CBL and CIPK gene families and their expressions in response to drought and arbuscular mycorrhizal fungi colonization","authors":"B. Shu, D. Cai, F. Zhang, D. J. Zhang, C. Y. Liu, WU Q.S., C. Luo","doi":"10.32615/bp.2020.123","DOIUrl":"https://doi.org/10.32615/bp.2020.123","url":null,"abstract":"The calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) complex is an essential calcium sensor and contributes to biotic and abiotic stress responses. However, citrus CBL and CIPK gene family members and their underlying roles during drought and arbuscular mycorrhizal fungi (AMF) colonization remain relatively unknown. In the present study, CBLs and CIPKs were characterized in Citrus sinensis by analyzing the presence of specific domains such as the elongation factor (EF)-hand motif in CBLs, and a protein kinase and an Asn-Ala-Phe domain in CIPKs. After mining the C. sinensis genome, we identified 8 CsCBLs and 17 CsCIPKs. Among these genes, three CsCBLs and nine CsCIPKs showed syntenic relationships with the Arabidopsis thaliana homologs AtCBLs and AtCIPKs, respectively. According to gene expression and cis-acting element analysis, all 8 CsCBLs and 16 CsCIPKs were expressed in the roots, where the regulation of expression was not consistent with their promoter cis-elements. Drought treatment remarkably downregulated the expression of CsCBL8 and upregulated CsCBL7, CsCIPK4, and CsCIPK7 expressions. The AMF colonization induced CsCBL4, 5, 6, and 7 as well as CsCIPK2, 4, 10, 11, 13, 14, and 16 expressions and repressed CsCBL1, 2, 3, and 8 and CsCIPK1, 3, 6, 8, 9, and 12 expressions. Based on the expression data and co-expression analysis, CsCBL1-CsCIPK1, CsCBL1-CsCIPK3, CsCBL1-CsCIPK6, and CsCBL1-CsCIPK9 showed the significant positive correlations to drought and AMF responses.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"773-783"},"PeriodicalIF":1.5,"publicationDate":"2020-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44873143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-specific responses of Populus deltoides to combined salinity and calcium under waterlogging conditions","authors":"Lingfeng Miao, D. Li, Fan Yang, Z. Tan","doi":"10.32615/bp.2020.116","DOIUrl":"https://doi.org/10.32615/bp.2020.116","url":null,"abstract":"In this study, we investigated the sex-specific ion uptake and physiological and biochemical responses to combined salinity and calcium treatments of male and female Populus deltoides under waterlogging conditions. Results indicate that both P. deltoides clones were sensitive to salinity and calcium. Under well-watered conditions, salinity stress could especially increase the Na content in female P. deltoides clones, whereas salinity and calcium could increase the K and Ca content in male clones. Waterlogging could evidently stimulate Na content in leaves under salinity, especially in female P. deltoides clones. However, waterlogging had no visible effects on the amount of Ca absorption between the two clones under abundant calcium conditions. The physiological and biochemical responses to combined salinity and calcium exceeded the effects of salinity or calcium individually. However, no significant difference was observed, hence indicating the similar responses of male and female P. deltoides clones. The lower Na content and Na:Ca ratio, and the higher K and Ca content in male clones suggest that the male clones could maintain ion homeostasis better than the female clones. The sex-specific differences in net photosynthetic rate, intercellular CO2 concentration, effective quantum yield of photosystem II, photochemical quenching coefficient, non-photochemical quenching coefficient, photosynthetic electron transport rate, content of superoxide anions, hydroxyl radicals, H2O2, soluble protein, and activities of superoxide dismutase under certain conditions suggest that female P. deltoides clones are more sensitive to salinity, calcium, and the combination of both than their male counterparts.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":"64 1","pages":"753-763"},"PeriodicalIF":1.5,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45883906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of ABC1 protein family members in Lepidium apetalum seeds and the expression of LaAbc1 in seedlings in response to abiotic stresses","authors":"Q. Yang, Z. Chen, H. Lu, H. Xie, J. Li, Y. Du, S. Han, H. Zhao, H. Zhao","doi":"10.32615/BP.2020.104","DOIUrl":"https://doi.org/10.32615/BP.2020.104","url":null,"abstract":"To study the biological function of activity of bcl complex (ABC1) proteins in Lepidium apetalum Willd., genes encoding ABC1 family proteins were identified from the seed transcriptome. The sequence most closely related to germination at a low temperature was selected and gene expressions in response to low temperature stress further studied. The results show that 21 ABC1 genes were expressed in seeds germinating at the low temperature: 4 genes were upregulated, 6 were downregulated, and 11 were not significantly different from controls. The results of fluorescence quantification of the low-temperature stress on the seedlings of 7-d-old L. apetalum showed that seven genes were up-regulated, six genes were down-regulated, and eight genes had no significant difference. Real-time quantitative PCR results show that under the low temperature stress, the expression of the LaAbc1-3 gene increased, but its expression decreased after some time. The expression of this gene increased again after removing the low temperature stress. The expression of LaAbc1-21 gene in L. apetalum seedlings showed a trend of decreasing first and then increasing. The LaAbc1-3 gene was insensitive to salt stress. Expression of the LaAbc1-21 gene was significantly up-regulated during the salt stress. Under osmotic stress, the expression of the LaAbc1-3 gene was down-regulated, and the expression was negatively correlated with polyethylene glycol (PEG-6000) concentration. Under the PEG-6000 treatment, the expression of the LaAbc1-21 gene was significantly up-regulated, and the expression was positively correlated with concentration. These results provide a basis for further analysis of the role of the ABC1 genes in the stress resistance of L. apetalum.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46010125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}