International journal of genetics and molecular biology最新文献_第7页

Genetic Variability Assessment in Irrigated Rice ( Oryza sativa and Oryza glaberrima ) by PCR-SSR in Mali 马里灌溉水稻(Oryza sativa和Oryza glaberrima)遗传变异的PCR-SSR评价

International journal of genetics and molecular biology Pub Date : 2018-05-22 DOI: 10.11648/j.ijgg.20180602.11

Sognan Dao, Halimatou Timbine, O. Goita, D. Traoré

{"title":"Genetic Variability Assessment in Irrigated Rice ( Oryza sativa and Oryza glaberrima ) by PCR-SSR in Mali","authors":"Sognan Dao, Halimatou Timbine, O. Goita, D. Traoré","doi":"10.11648/j.ijgg.20180602.11","DOIUrl":"https://doi.org/10.11648/j.ijgg.20180602.11","url":null,"abstract":"Rice species (Oryza sativa, L. and Oryza glaberrima, Steud) provide 20% energy of world’s food. Knowledge of genetic variability is important to remove duplicate materials for gene bank management and conservation. In this study, 59 microsatellite markers were used to assess the genetic diversity of 54 intraspecific (Oryza sativa) and interspecific (Oryza sativa X Oryza glaberrima) irrigated rice varieties by PCR-SSR. A total of 250 alleles were detected with an average of 4.24 alleles per SSR. Genetic diversity was ranged from 0.0713 (RM333, RM3744) to 0.8937 (RM251) to with an average of 0.4325. Polymorphism Information Content (PIC) varied from 0.0688 (RM333, RM3744) to 0.8854 (RM251) with an average of 0.3940. Rice genotypes were shared between five Groups based on their similarity with majority of them (64.81%) under Group V. Variety SK 7-8 within Group III was highly dissimilar to other varieties. Malian varieties were shared out between Groups II, III, VI and V. Strong genetic variability was observed within cluster V2 of Group V among the most cultivated and appreciated rice varieties such as Kogoni 91-1, Adny11 and BG90-2. Allelic variability observed among rice varieties could help breeding programs to identify cultivars with good agronomic traits for crop improvement.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"1 1","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2018-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79942833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Systematic Genetic Assessment of ARFGEF2 Mutations in Periventricular Heterotopia 心室周围异位ARFGEF2突变的系统遗传评估

International journal of genetics and molecular biology Pub Date : 2018-05-05 DOI: 10.11648/J.IJGG.20180601.13

L. M. A. Neghery, R. Kenana, Albandary Al Bakheet, Rawan Al Mass, Faten Al Mutairi, Maysoon Al Sagob, A. Qari, Rozeena Huma, D. Çolak, M. Daghestani, N. Kaya, Moeen Al‐ Sayed

{"title":"A Systematic Genetic Assessment of ARFGEF2 Mutations in Periventricular Heterotopia","authors":"L. M. A. Neghery, R. Kenana, Albandary Al Bakheet, Rawan Al Mass, Faten Al Mutairi, Maysoon Al Sagob, A. Qari, Rozeena Huma, D. Çolak, M. Daghestani, N. Kaya, Moeen Al‐ Sayed","doi":"10.11648/J.IJGG.20180601.13","DOIUrl":"https://doi.org/10.11648/J.IJGG.20180601.13","url":null,"abstract":"Mutations in ADP-ribosylation factor guanine nucleotide-exchange factor 2 (ARFGEF2) lead to autosomal recessive periventricular heterotopia (PH). To date, 11 mutations, (six missense mutations, one splicing mutation, one small deletion, two small insertions, and one small deletion/insertion) have been reported. Assessing ARFGEF2 mutations will provide a holistic overview of PH. This retrospective study was conducted in 2016 at King Faisal Specialist Hospital and Research Center. For the index patient, magnetic resonance imaging studies revealed a symmetrical focal hyperintensity involving the putamen bilaterally and the inner aspect of the globus pallidus. After family members were genotyped, an autozygosity analysis was performed, followed by exome sequencing of the index patient; A comprehensive filtering approach based on the loss of heterozygosity (LOH) was used to identify variants in phenotypically relevant genes. We report a consanguineous family with two affected individuals, a boy and a girl, with a history of microcephaly, global developmental delay, intellectual disability, myoclonic seizure, and dystonia. The patients carried a novel nonsense mutation (c.3974G>A, p. Trp1325*) in the Armadillo-type fold domain of ARFGEF2. These findings extend our understanding of the phenotype–genotype correlations for ARFGEF2 mutations.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"51 1","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2018-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86644387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Small Pure 3q25.1 Duplication Associated with Multiple Cerebral Organizational Defects 一个小的纯3q25.1重复与多种大脑组织缺陷相关

International journal of genetics and molecular biology Pub Date : 2018-05-05 DOI: 10.11648/j.ijgg.20180601.14

Jesus David Sendoya Vargas, Divya Periasamy, F. Sánchez, Maria Alejandra Benavides Fierro, Ingrid Carolina Duran Palacios, R. Sidlow, Henry Ostos Alfonso

引用次数: 0

GENETIC VARIABILITY AND CHARACTER ASSOCIATION IN SOYBEAN GERMPLASM FOR PRE-HARVEST SPROUTING TOLERANCE AND ASSOCIATED TRAITS 大豆种质收获前发芽耐受性及相关性状的遗传变异与性状关联

International journal of genetics and molecular biology Pub Date : 2018-04-30 DOI: 10.9735/0975-2862.10.4.390-393

A. Sharma, M. Sarma, N. Ramchiary

引用次数: 1

Use of expressed sequence tags-derived simple sequence repeat (SSR) markers for population studies of released and elite sweet potato 表达序列标签衍生的简单序列重复(SSR)标记在甘薯释放和优质群体研究中的应用

International journal of genetics and molecular biology Pub Date : 2018-04-30 DOI: 10.5897/IJGMB2017.0159

M. Quain, K. Adofo, D. Appiah-Kubi, R. Prempeh, J. Asafu-Agyei, Belinda Akomeah, Harrison Kwame Dapaah

{"title":"Use of expressed sequence tags-derived simple sequence repeat (SSR) markers for population studies of released and elite sweet potato","authors":"M. Quain, K. Adofo, D. Appiah-Kubi, R. Prempeh, J. Asafu-Agyei, Belinda Akomeah, Harrison Kwame Dapaah","doi":"10.5897/IJGMB2017.0159","DOIUrl":"https://doi.org/10.5897/IJGMB2017.0159","url":null,"abstract":"Development of improved varieties of sweet potato to meet specific end users in the sub-Saharan African region has the potential of transforming the crop’s market, consumption and health benefits. Molecular biology techniques such as fingerprinting are key to crop improvement, and critical for numerous genetic studies. This study, carried out in Ghana, seeks to determine genetic relatedness among two populations of sweet potato (released and elite), as well as to identify polymorphic and informative markers for diversity studies. Twenty expressed sequence tags-simple sequence repeat (EST-SSR) markers were used for analysis of DNA assembled. A total of 169 alleles were obtained, with an average of 8.5 alleles per locus. Pairwise genetic distance among genotypes ranged from 12 – 89. GenAlex v6.4 analysis showed that a higher genetic diversity existed within elite genotypes than the released lines. Results analyzed using Popgene version 1.31 software indicated that on the basis of both populations studied, EST-SSR markers utilized were very polymorphic (95%) and showed genetic diversity (I =0.888, He= 0.548) suggesting high heterozygosity in the collected varieties. The overall Fit (0.372%) and Fis (0.088%) suggest high genetic diversity within populations, and gene flow (1.95) indicate an exchange of genes between released sweet potatoes and elite ones. Ewens-Watterson test was conducted to test the neutrality. From the results obtained, markers CB329940, CB330477 and CB330936 showed high allelic polymorphism among varieties used and were recommended to be included in diversity studies. \u0000 \u0000 \u0000 \u0000 Key words: Genetic diversity, genetic relatedness, expressed sequence tags-simple sequence repeat (EST-SSR), populations, released sweet potato, elite sweet potato.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"26 1","pages":"14-25"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75533533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

PERFORMANCE OF STRAWBERRY F1 HYBRIDS AND PARENTS FOR FLOWERING, FRUITING AND VEGETATIVE PARAMETERS 草莓f1杂交种和亲本在开花、结果和营养参数方面的表现

International journal of genetics and molecular biology Pub Date : 2018-04-30 DOI: 10.9735/0975-2862.10.4.387-389

Deepika, G. Sharma

引用次数: 0

Genetic Confirmation of Mungbean Genotypes (Vigna Radiata L. Wilczek) Using Molecular Markers 绿豆(Vigna Radiata L. Wilczek)基因型的分子标记遗传鉴定

International journal of genetics and molecular biology Pub Date : 2018-04-15 DOI: 10.9735/0975-2862.10.3.365-369

A. Nath, S. Maloo, Devendra Jain, S. Nath, A. Chakma, R. Verma

{"title":"Genetic Confirmation of Mungbean Genotypes (Vigna Radiata L. Wilczek) Using Molecular Markers","authors":"A. Nath, S. Maloo, Devendra Jain, S. Nath, A. Chakma, R. Verma","doi":"10.9735/0975-2862.10.3.365-369","DOIUrl":"https://doi.org/10.9735/0975-2862.10.3.365-369","url":null,"abstract":"Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes. Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied method is marker analysis. RAPD and ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes. Evaluation of genetic diversity would promote the efficient use of genetic variations, effective conservation and purity of the genotype to be determined as well as utilization of germplasm in crop improvement. The RAPD and ISSR data were evaluated to obtain a combined similarity matrix. The similarity coefficient values lay between 0.46–0.68. The RAPD and ISSR cluster tree analysis showed that the eight genotypes could be divided into 4 clusters. The genotype BM 4 was grouped in separate VI cluster. However, PDM 139 was grouped on cluster IIA. In the light of RAPD and ISSR study the parents of the cross BM 4 x PDM 139 were also noticed for their genetic diversity, having 53% dissimilarity and grouped into the separate clusters.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"5 1","pages":"365"},"PeriodicalIF":0.0,"publicationDate":"2018-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81408059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Estimates of Genetic Component of Variances and Genetic Ratios for Earliness and Yield Attributing Traits in Cucumber 黄瓜早熟和产量性状遗传方差和遗传比的估计

International journal of genetics and molecular biology Pub Date : 2018-04-15 DOI: 10.9735/0975-2862.10.3.373-375

T. Bhutia, A. Munshi, A. K. Sureja, B. Gurung, S. Singh

引用次数: 0

Phylogenetic Relationship among the Wild Rice (Oryza Nivara) and Intraspecies of Rice Genotypes Belonging to Chhattisgarh Revealed by Chloroplast Dna Regions psbA-trnH 利用叶绿体Dna psbA-trnH区揭示野生稻与恰蒂斯加尔邦稻种内基因型的系统发育关系

International journal of genetics and molecular biology Pub Date : 2018-04-15 DOI: 10.9735/0975-2862.10.3.376-379

Jyoti Singh, Shubha Banerjee

引用次数: 0

Role of Osteopontin in Bovines and their Association with Different Traits: A Review 骨桥蛋白在牛体内的作用及其与不同性状的关系

International journal of genetics and molecular biology Pub Date : 2018-04-15 DOI: 10.9735/0975-2862.10.3.370-372

Suchit Kumar, A. Mukherjee, Sunil Kumar, A. Yadav, N. Kumar

引用次数: 1