Human genomics and proteomics : HGP最新文献

Plasma Proteomic Signature in Overweight Girls Closely Correlates with Homeostasis Model Assessment (HOMA), an Objective Measure of Insulin Resistance. 超重女孩的血浆蛋白质组学特征与体内平衡模型评估(HOMA)密切相关，这是胰岛素抵抗的客观指标。

Human genomics and proteomics : HGP Pub Date : 2011-01-01 Epub Date: 2011-10-06 DOI: 10.4061/2011/323629

Stephen W Rothwell, Merrily Poth, Harkirtin McIver, Chiedozie Ayika, Ofer Eidelman, Catherine Jozwik, Harvey B Pollard

{"title":"Plasma Proteomic Signature in Overweight Girls Closely Correlates with Homeostasis Model Assessment (HOMA), an Objective Measure of Insulin Resistance.","authors":"Stephen W Rothwell, Merrily Poth, Harkirtin McIver, Chiedozie Ayika, Ofer Eidelman, Catherine Jozwik, Harvey B Pollard","doi":"10.4061/2011/323629","DOIUrl":"https://doi.org/10.4061/2011/323629","url":null,"abstract":"Obesity is known to be associated with a large number of long-term morbidities, and while in some cases the relationship of obesity and the consequences is clear (for example, excess weight and lower extremity orthopedic problems) in others the mechanism is not as clear. One common system of categorizing overweight in terms of the likelihood of negative consequences involves using the concept of \"metabolic syndrome\". We hypothesized that the development of a plasma protein profile of overweight adolescents with and without the metabolic syndrome might give a more precise and informative picture of the disease process than the current clinical categorization and permit early targeted intervention. For this paper, we used antibody microarrays to analyze the plasma proteome of a group of 15 overweight female adolescent patients. Upon analysis of the proteome, the overweight patients diverged from the nonoverweight female controls. Furthermore, the overweight patients were divided by the analysis into two population clusters, each with distinctive protein expression patterns. Interestingly, the clusters were characterized by differences in insulin resistance, as measured by HOMA. Categorization according to the presence or absence of the metabolic syndrome did not yield such clusters.","PeriodicalId":88887,"journal":{"name":"Human genomics and proteomics : HGP","volume":"2011 ","pages":"323629"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2011/323629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30523147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Pharmacogenetics of anticoagulants. 抗凝血药物遗传学。

Human genomics and proteomics : HGP Pub Date : 2010-09-13 DOI: 10.4061/2010/754919

Anders Rane, Jonatan D Lindh

引用次数: 19

Genome-wide linkage and association scans for quantitative trait Loci of serum lactate dehydrogenase-the framingham heart study. 血清乳酸脱氢酶数量性状位点的全基因组连锁和关联扫描——framingham心脏研究。

Human genomics and proteomics : HGP Pub Date : 2010-09-01 DOI: 10.4061/2010/905237

Jing-Ping Lin, Gang Zheng, Jungnam Joo, L Adrienne Cupples

引用次数: 0

Pharmacogenomics of mood stabilizers in the treatment of bipolar disorder. 情绪稳定剂治疗双相情感障碍的药物基因组学研究。

Human genomics and proteomics : HGP Pub Date : 2010-08-03 DOI: 10.4061/2010/159761

Alessio Squassina, Mirko Manchia, Maria Del Zompo

引用次数: 24

Developmental pharmacogenetics in pediatric rheumatology: utilizing a new paradigm to effectively treat patients with juvenile idiopathic arthritis with methotrexate. 儿童风湿病学的发育药物遗传学:利用甲氨蝶呤有效治疗青少年特发性关节炎的新范例。

Human genomics and proteomics : HGP Pub Date : 2010-06-22 DOI: 10.4061/2010/257120

Mara L Becker, J Steven Leeder

引用次数: 14

Genetic variations in human glutathione transferase enzymes: significance for pharmacology and toxicology. 人类谷胱甘肽转移酶的遗传变异:药理学和毒理学意义。

Human genomics and proteomics : HGP Pub Date : 2010-06-13 DOI: 10.4061/2010/876940

P David Josephy

引用次数: 157

Gender dependence for a subset of the low-abundance signaling proteome in human platelets. 人类血小板中低丰度信号蛋白组子集的性别依赖性。

Human genomics and proteomics : HGP Pub Date : 2010-04-13 DOI: 10.4061/2010/164906

Ofer Eidelman, Catherine Jozwik, Wei Huang, Meera Srivastava, Stephen W Rothwell, David M Jacobowitz, Xiaoduo Ji, Xiuying Zhang, William Guggino, Jerry Wright, Jeffrey Kiefer, Cara Olsen, Nima Adimi, Gregory P Mueller, Harvey B Pollard

{"title":"Gender dependence for a subset of the low-abundance signaling proteome in human platelets.","authors":"Ofer Eidelman, Catherine Jozwik, Wei Huang, Meera Srivastava, Stephen W Rothwell, David M Jacobowitz, Xiaoduo Ji, Xiuying Zhang, William Guggino, Jerry Wright, Jeffrey Kiefer, Cara Olsen, Nima Adimi, Gregory P Mueller, Harvey B Pollard","doi":"10.4061/2010/164906","DOIUrl":"https://doi.org/10.4061/2010/164906","url":null,"abstract":"The incidence of cardiovascular diseases is ten-times higher in males than females, although the biological basis for this gender disparity is not known. However, based on the fact that antiplatelet drugs are the mainstay for prevention and therapy, we hypothesized that the signaling proteomes in platelets from normal male donors might be more activated than platelets from normal female donors. We report here that platelets from male donors express significantly higher levels of signaling cascade proteins than platelets from female donors. In silico connectivity analysis shows that the 24 major hubs in platelets from male donors focus on pathways associated with megakaryocytic expansion and platelet activation. By contrast, the 11 major hubs in platelets from female donors were found to be either negative or neutral for platelet-relevant processes. The difference may suggest a biological mechanism for gender discrimination in cardiovascular disease.","PeriodicalId":88887,"journal":{"name":"Human genomics and proteomics : HGP","volume":"2010 ","pages":"164906"},"PeriodicalIF":0.0,"publicationDate":"2010-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2010/164906","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29380197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Proteomics: challenges, techniques and possibilities to overcome biological sample complexity. 蛋白质组学:克服生物样品复杂性的挑战、技术和可能性。

Human genomics and proteomics : HGP Pub Date : 2009-12-08 DOI: 10.4061/2009/239204

Kondethimmanahalli Chandramouli, Pei-Yuan Qian

{"title":"Proteomics: challenges, techniques and possibilities to overcome biological sample complexity.","authors":"Kondethimmanahalli Chandramouli, Pei-Yuan Qian","doi":"10.4061/2009/239204","DOIUrl":"https://doi.org/10.4061/2009/239204","url":null,"abstract":"Proteomics is the large-scale study of the structure and function of proteins in complex biological sample. Such an approach has the potential value to understand the complex nature of the organism. Current proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. Advances in protein fractionation and labeling techniques have improved protein identification to include the least abundant proteins. In addition, proteomics has been complemented by the analysis of posttranslational modifications and techniques for the quantitative comparison of different proteomes. However, the major limitation of proteomic investigations remains the complexity of biological structures and physiological processes, rendering the path of exploration paved with various difficulties and pitfalls. The quantity of data that is acquired with new techniques places new challenges on data processing and analysis. This article provides a brief overview of currently available proteomic techniques and their applications, followed by detailed description of advantages and technical challenges. Some solutions to circumvent technical difficulties are proposed.","PeriodicalId":88887,"journal":{"name":"Human genomics and proteomics : HGP","volume":"2009 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2009/239204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29352172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 378

Development of Potential Pharmacodynamic and Diagnostic Markers for Anti-IFN-α Monoclonal Antibody Trials in Systemic Lupus Erythematosus. 系统性红斑狼疮抗ifn -α单克隆抗体潜在药效学和诊断标志物的研究。

Human genomics and proteomics : HGP Pub Date : 2009-11-17 DOI: 10.4061/2009/374312

Yihong Yao, Brandon W Higgs, Chris Morehouse, Melissa de Los Reyes, Wendy Trigona, Philip Brohawn, Wendy White, Jianliang Zhang, Barbara White, Anthony J Coyle, Peter A Kiener, Bahija Jallal

{"title":"Development of Potential Pharmacodynamic and Diagnostic Markers for Anti-IFN-α Monoclonal Antibody Trials in Systemic Lupus Erythematosus.","authors":"Yihong Yao, Brandon W Higgs, Chris Morehouse, Melissa de Los Reyes, Wendy Trigona, Philip Brohawn, Wendy White, Jianliang Zhang, Barbara White, Anthony J Coyle, Peter A Kiener, Bahija Jallal","doi":"10.4061/2009/374312","DOIUrl":"https://doi.org/10.4061/2009/374312","url":null,"abstract":"To identify potential pharmacodynamic biomarkers to guide dose selection in clinical trials using anti-interferon-alpha (IFN-α) monoclonal antibody (mAb) therapy for systemic lupus erythematosus (SLE), we used an Affymetrix human genome array platform and identified 110 IFN-α/β-inducible transcripts significantly upregulated in whole blood (WB) of 41 SLE patients. The overexpression of these genes was confirmed prospectively in 54 additional SLE patients and allowed for the categorization of the SLE patients into groups of high, moderate, and weak overexpressers of IFN-α/β-inducible genes. This approach could potentially allow for an accurate assessment of drug target neutralization in early trials of anti-IFN-α mAb therapy for SLE. Furthermore, ex vivo stimulation of healthy donor peripheral blood mononuclear cells with SLE patient serum and subsequent neutralization with anti-IFN-α mAb or anti-IFN-α receptor mAb showed that anti-IFN-α mAb has comparable effects of neutralizing the overexpression of type I IFN-inducible genes as that of anti-IFNAR mAb. These results suggest that IFN-α, and not other members of type I IFN family in SLE patients, is mainly responsible for the induction of type I IFN-inducible genes in WB of SLE patients. Taken together, these data strengthen the view of IFN-α as a therapeutic target for SLE.","PeriodicalId":88887,"journal":{"name":"Human genomics and proteomics : HGP","volume":"2009 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2009/374312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29352169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 143

Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration. WNT/PCP、Flt-3L/Flt-3和CXCL9/CXCR3是低分期CLL细胞增殖、存活和迁移的调节因子

Human genomics and proteomics : HGP Pub Date : 2009-06-24 DOI: 10.4061/2009/453634

Daruka Mahadevan, James Choi, Laurence Cooke, Bram Simons, Christopher Riley, Thomas Klinkhammer, Rohit Sud, Sirisha Maddipoti, Sean Hehn, Harinder Garewal, Catherine Spier

{"title":"Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration.","authors":"Daruka Mahadevan, James Choi, Laurence Cooke, Bram Simons, Christopher Riley, Thomas Klinkhammer, Rohit Sud, Sirisha Maddipoti, Sean Hehn, Harinder Garewal, Catherine Spier","doi":"10.4061/2009/453634","DOIUrl":"https://doi.org/10.4061/2009/453634","url":null,"abstract":"Gene expression profiling (GEP) of 8 stage 0/I untreated Chronic Lymphocytic Leukemia (CLL) patients showed over-expression of Frizzled 3 (FZD3)/ROR-1 receptor tyrosine kinase (RTK), FLT-3 RTK and CXCR3 G-protein coupled receptor (GPCR). RT-PCR of 24 genes in 21 patients of the WNT pathway corroborated the GEP. Transforming growth factorβ, fibromodulin, TGFβRIII and SMAD2 are also over-expressed by GEP. Serum cytokine profiling of 26 low stage patients showed elevation of IFNγ, CSF3, Flt-3L and insulin-like growth factor binding protein 4. In order to ascertain why CLL cells grow poorly in culture, a GEP of 4 CLL patients cells at 0 hr and 24 hr in culture demonstrated over expression of CXCL5, CCL2 and CXCL3, that may recruit immune cells for survival. Treatment with thalidomide, an immunomodulatory agent, showed elevation of CCL5 by GEP but was not cytotoxic to CLL cells. Our data suggest an interplay of several oncogenic pathways, cytokines and immune cells that promote a survival program in CLL.","PeriodicalId":88887,"journal":{"name":"Human genomics and proteomics : HGP","volume":"2009 ","pages":"453634"},"PeriodicalIF":0.0,"publicationDate":"2009-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2009/453634","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29390867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22