Dong wu xue yan jiu = Zoological research最新文献

Yunnan snub-nosed monkey research of the Kunming Institute of Zoology, Chinese Academy of Sciences. 中国科学院昆明动物研究所云南仰鼻猴研究。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.189

Yuan-Ye Ma

引用次数: 0

The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models. 基因组编辑技术的大爆炸:CRISPR/Cas9系统在疾病动物模型中的开发与应用

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.191

Ming Shao, Tian-Rui Xu, Ce-Shi Chen

引用次数: 18

Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina). 北方长尾猕猴APOBEC3s的分子克隆及抗hiv -1活性研究

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.246

Xiao-Liang Zhang, Jia-Hao Song, Wei Pang, Yong-Tang Zheng

{"title":"Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina).","authors":"Xiao-Liang Zhang, Jia-Hao Song, Wei Pang, Yong-Tang Zheng","doi":"10.13918/j.issn.2095-8137.2016.4.246","DOIUrl":"https://doi.org/10.13918/j.issn.2095-8137.2016.4.246","url":null,"abstract":"Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection. ","PeriodicalId":88862,"journal":{"name":"Dong wu xue yan jiu = Zoological research","volume":"37 4","pages":"246-51"},"PeriodicalIF":0.0,"publicationDate":"2016-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34713183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Advances and perspectives in the application of CRISPR/Cas9 in insects. 在昆虫中应用 CRISPR/Cas9 的进展和前景。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.220

Lei Chen, Gui Wang, Ya-Nan Zhu, Hui Xiang, Wen Wang

{"title":"Advances and perspectives in the application of CRISPR/Cas9 in insects.","authors":"Lei Chen, Gui Wang, Ya-Nan Zhu, Hui Xiang, Wen Wang","doi":"10.13918/j.issn.2095-8137.2016.4.220","DOIUrl":"10.13918/j.issn.2095-8137.2016.4.220","url":null,"abstract":"Insects compose more than half of all living organisms on earth, playing essential roles in global ecosystems and forming complex relationships with humans. Insect research has significant biological and practical importance. However, the application of genetic manipulation technology has long been restricted to several model insects only, such as gene knockout in Drosophila, which has severely restrained the development of insect biology research. Recently, with the increase in the release of insect genome data and the introduction of the CRISPR/Cas9 system for efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species. Here, we summarize the advances in CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application in future insect studies. This review provides detailed information about the application of the CRISPR/Cas9 system in insect research and presents possible ways to improve its use in functional studies and insect pest control. ","PeriodicalId":88862,"journal":{"name":"Dong wu xue yan jiu = Zoological research","volume":"37 4","pages":"220-8"},"PeriodicalIF":0.0,"publicationDate":"2016-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34713180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of the genome editing tool CRISPR/Cas9 in non-human primates. 基因组编辑工具CRISPR/Cas9在非人灵长类动物中的应用

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.214

Xin Luo, Min Li, Bing Su

引用次数: 30

Research proceedings on amphibian model organisms. 关于两栖动物模式生物的研究论文集。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.237

Lu-Sha Liu, Lan-Ying Zhao, Shou-Hong Wang, Jian-Ping Jiang

引用次数: 0

Modeling postpartum depression in rats: theoretic and methodological issues. 大鼠产后抑郁模型:理论和方法问题。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.229

Ming Li, Shinn-Yi Chou

{"title":"Modeling postpartum depression in rats: theoretic and methodological issues.","authors":"Ming Li, Shinn-Yi Chou","doi":"10.13918/j.issn.2095-8137.2016.4.229","DOIUrl":"https://doi.org/10.13918/j.issn.2095-8137.2016.4.229","url":null,"abstract":"The postpartum period is when a host of changes occur at molecular, cellular, physiological and behavioral levels to prepare female humans for the challenge of maternity. Alteration or prevention of these normal adaptions is thought to contribute to disruptions of emotion regulation, motivation and cognitive abilities that underlie postpartum mental disorders, such as postpartum depression. Despite the high incidence of this disorder, and the detrimental consequences for both mother and child, its etiology and related neurobiological mechanisms remain poorly understood, partially due to the lack of appropriate animal models. In recent decades, there have been a number of attempts to model postpartum depression disorder in rats. In the present review, we first describe clinical symptoms of postpartum depression and discuss known risk factors, including both genetic and environmental factors. Thereafter, we discuss various rat models that have been developed to capture various aspects of this disorder and knowledge gained from such attempts. In doing so, we focus on the theories behind each attempt and the methods used to achieve their goals. Finally, we point out several understudied areas in this field and make suggestions for future directions. ","PeriodicalId":88862,"journal":{"name":"Dong wu xue yan jiu = Zoological research","volume":"37 4","pages":"229-36"},"PeriodicalIF":0.0,"publicationDate":"2016-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34713181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Early embryonic development and transplantation in tree shrews. 树鼩的早期胚胎发育和移植。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.252

Lan-Zhen Yan, Bin Sun, Long-Bao Lyu, Yu-Hua Ma, Jia-Qi Chen, Qing Lin, Ping Zheng, Xu-Dong Zhao

{"title":"Early embryonic development and transplantation in tree shrews.","authors":"Lan-Zhen Yan, Bin Sun, Long-Bao Lyu, Yu-Hua Ma, Jia-Qi Chen, Qing Lin, Ping Zheng, Xu-Dong Zhao","doi":"10.13918/j.issn.2095-8137.2016.4.252","DOIUrl":"https://doi.org/10.13918/j.issn.2095-8137.2016.4.252","url":null,"abstract":"As a novel experimental animal model, tree shrews have received increasing attention in recent years. Despite this, little is known in regards to the time phases of their embryonic development. In this study, surveillance systems were used to record the behavior and timing of copulations; embryos at different post-copulation stages were collected and cultured in vitro; and the developmental characteristics of both early-stage and in vitro cultured embryos were determined. A total of 163 females were collected following effective copulation, and 150 were used in either unilateral or bilateral oviduct embryo collections, with 307 embryos from 111 females obtained (conception rate=74%). Among them, 237 embryos were collected from 78 females, bilaterally, i.e., the average embryo number per female was 3.04; 172 fertilized eggs collected from 55 females, bilaterally, were cultured for 24-108 h in vitro for developmental observations; finally, 65 embryos from 23 bilateral cases and 70 embryos from 33 unilateral cases were used in embryo transplantation. ","PeriodicalId":88862,"journal":{"name":"Dong wu xue yan jiu = Zoological research","volume":"37 4","pages":"252-8"},"PeriodicalIF":0.0,"publicationDate":"2016-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34713184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. 使用CRISPR/Cas9和单倍体胚胎干细胞系统产生转基因小鼠。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-07-18 DOI: 10.13918/j.issn.2095-8137.2016.4.205

Li-Fang Jin, Jin-Song Li

引用次数: 16

Expression analysis of eight amphioxus genes involved in the Wnt/β-catenin signaling pathway. 文昌鱼Wnt/β-catenin信号通路8个相关基因的表达分析。

Dong wu xue yan jiu = Zoological research Pub Date : 2016-05-18 DOI: 10.13918/j.issn.2095-8137.2016.3.136

Jing Wang, Guang Li, Guang-Hui Qian, Jun-Hao Hua, Yi-Quan Wang

{"title":"Expression analysis of eight amphioxus genes involved in the Wnt/β-catenin signaling pathway.","authors":"Jing Wang, Guang Li, Guang-Hui Qian, Jun-Hao Hua, Yi-Quan Wang","doi":"10.13918/j.issn.2095-8137.2016.3.136","DOIUrl":"https://doi.org/10.13918/j.issn.2095-8137.2016.3.136","url":null,"abstract":"The Wnt/β-catenin signaling pathway plays a crucial role in the embryonic development of metazoans. Although the pathway has been studied extensively in many model animals, its function in amphioxus, the most primitive chordate, remains largely uncharacterized. To obtain basic data for functional analysis, we identified and isolated seven genes (Lrp5/6, Dvl, APC, CkIα, CkIδ, Gsk3β, and Gro) of the Wnt/β-catenin signaling pathway from the amphioxus (Branchiostoma floridae) genome. Phylogenetic analysis revealed that amphioxus had fewer members of each gene family than that found in vertebrates. Whole-mount in situ hybridization showed that the genes were maternally expressed and broadly distributed throughout the whole embryo at the cleavage and blastula stages. Among them, Dvl was expressed asymmetrically towards the animal pole, while the others were evenly distributed in all blastomeres. At the mid-gastrula stage, the genes were specifically expressed in the primitive endomesoderm, but displayed different patterns. When the embryo developed into the neurula stage, the gene expressions were mainly detected in either paraxial somites or the tail bud. With the development of the embryo, the expression levels further decreased gradually and remained only in some pharyngeal regions or the tail bud at the larva stage. Our results suggest that the Wnt/β-catenin pathway might be involved in amphioxus somite formation and posterior growth, but not in endomesoderm specification. ","PeriodicalId":88862,"journal":{"name":"Dong wu xue yan jiu = Zoological research","volume":"37 3","pages":"136-43"},"PeriodicalIF":0.0,"publicationDate":"2016-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914576/pdf/ZoolRes-37-3-136.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34614617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5