The open reproductive science journal最新文献

Genetic Variation in the Testis-Specific Poly(A) Polymerase Beta(PAPOLB) Gene Among Japanese Males 日本男性睾丸特异性聚(A)聚合酶β (PAPOLB)基因的遗传变异

The open reproductive science journal Pub Date : 2015-02-24 DOI: 10.2174/1874255601507010001

Hiromitsu Tanaka, A. Tsujimura, Y. Miyagawa, M. Wada

引用次数: 3

Present and Active: Transglutaminases in the Virgin Rat Uterus andCervix 存在的和活跃的:处女大鼠子宫和子宫颈中的谷氨酰胺转氨酶

The open reproductive science journal Pub Date : 2014-07-25 DOI: 10.2174/1874255620140708001

Lindsey W. Young, J. Thompson, K. Hitomi, S. Watts

引用次数: 0

Effects of Nitric Oxide Exposure on Human Sperm Function and Apoptosis Markers 一氧化氮暴露对人类精子功能和凋亡标志物的影响

The open reproductive science journal Pub Date : 2014-06-27 DOI: 10.2174/1874255601406010017

F. Lampiao, J. Huussen, S. Plessis

引用次数: 1

Ultrastructural and Aneuploidy Studies in Sperm from Patients with His- tory of Consanguinity 血亲史患者精子超微结构及非整倍体研究

The open reproductive science journal Pub Date : 2014-06-27 DOI: 10.2174/1874255601406010021

E. Moretti, G. Collodel

{"title":"Ultrastructural and Aneuploidy Studies in Sperm from Patients with His- tory of Consanguinity","authors":"E. Moretti, G. Collodel","doi":"10.2174/1874255601406010021","DOIUrl":"https://doi.org/10.2174/1874255601406010021","url":null,"abstract":"The data on the role of consanguinity in male infertility are scarce. The presence of systematic sperm defects in consanguineous respect to non-consanguineous population was demonstrated. Systematic defects are characterized by a specific alteration present in sperm for the whole life of the carrier. This study was aimed at exploring the sperm quality in selected infertile consanguineous patients (group 3) with non- systematic sperm defects, compared with that of infertile non-consanguineous patients (group 2) and that of proven fertil- ity subjects (group 1). Sperm analysis was performed by light and transmission electron microscopy (TEM). TEM data was mathematically elaborated. Fluorescence in situ hybridization (FISH) analysis for chromosome 18, X and Y was per- formed in sperm nuclei for aneuploidy evaluation. The progressive motility was reduced in groups 2 (p < 0.05) and 3 (p < 0.01), compared to group 1, and in group 3 versus group 2 (p < 0.01). Regarding TEM scores, fertility index was re- duced in group 3 (p < 0.01); the percentages of sperm apoptosis, necrosis and immaturity were significantly higher in groups 2 and 3 versus group 1 and in group 3 vs. group 2 (except for necrosis). FISH analysis showed increased frequency of diploidy (p < 0.01) in groups 2 and 3 vs. group 1. The consanguinity seems to influence the spermatogenetic process from a morphological point of view, by reducing the motility and fertility index and increasing sperm apoptosis and immaturity; chromosome meiotic segregation was not af- fected by the condition of consanguinity.","PeriodicalId":88757,"journal":{"name":"The open reproductive science journal","volume":"27 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2014-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83665615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of PCNA, Caspase 3 and E-cadherin on the Ventral Prostate of Soy Treated Rats PCNA、Caspase 3和E-cadherin对大豆处理大鼠腹侧前列腺的影响

The open reproductive science journal Pub Date : 2014-06-13 DOI: 10.2174/1874255601406010008

B. Oltra, J. Pozuelo, R. Rodríguez, I. Ingelmo, R. Arriazu, L. Santamaría

{"title":"Evaluation of PCNA, Caspase 3 and E-cadherin on the Ventral Prostate of Soy Treated Rats","authors":"B. Oltra, J. Pozuelo, R. Rodríguez, I. Ingelmo, R. Arriazu, L. Santamaría","doi":"10.2174/1874255601406010008","DOIUrl":"https://doi.org/10.2174/1874255601406010008","url":null,"abstract":"The incidence of certain cancers, including prostate cancer, is considerably higher in western countries than in Southeast Asia. Many studies have linked soy consumption to the lower incidence of prostate cancer in these countries. Twenty male Sprague-Dawley rats were randomized and divided into a control group and a group treated with soy. The ventral prostates of each animal were sectioned and stained with hematoxilin-eosin for morphological description and immunostained to detect PCNA, cleaved caspase 3 and E-cadherin immunoreactivities. Estimates of the number of total epithelial cells and the number of epithelial cells immunoreactive to PCNA and cleaved caspase 3 were calculated using the optical disector technique. Measurement of E-cadherin was carried out by calculating the volume fraction of epithe- lium immunostained by E-cadherin. Soy treated group showed atrophy in the epithelium and a diminished expression of PCNA, cleaved caspase 3 and E-cadherin, which means there is a reduced cell proliferation, apoptosis through caspases way and cell adherence. It can be concluded that soy treatment induces atrophy in the epithelium by reducing cell prolif- eration.","PeriodicalId":88757,"journal":{"name":"The open reproductive science journal","volume":"298 1","pages":"8-16"},"PeriodicalIF":0.0,"publicationDate":"2014-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77507454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Improved In Vitro Fertilization Ability of Mouse Sperm Caused by the Addition of Licorice Extract to the Preincubation Medium 在培养前培养基中添加甘草提取物提高小鼠精子的体外受精能力

The open reproductive science journal Pub Date : 2014-05-30 DOI: 10.2174/1874255601406010001

Naguyen H. Tung, Y. Shoyama, M. Wada, Hiromitsu Tanaka

引用次数: 12

Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice 二甲双胍可预防脱氢表雄酮引起的早孕小鼠一氧化氮和脂质过氧化升高

The open reproductive science journal Pub Date : 2013-09-20 DOI: 10.2174/1874255620130913002

C. Luchetti, D. Paz, A. Motta

{"title":"Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice","authors":"C. Luchetti, D. Paz, A. Motta","doi":"10.2174/1874255620130913002","DOIUrl":"https://doi.org/10.2174/1874255620130913002","url":null,"abstract":"The aim of this work was to study the effects of dehydroepiandrosterone (DHEA) and metformin (M) on nitric oxide (NO) system and oxidative stress in embryo implantation sites of early pregnant mice. The biguanide M is used for treating polycystic ovary syndrome but its complete mechanism of action remains unknown. Nitric oxide (NO) has impor- tant protective roles during pregnancy, keeping uterine relaxation and vascular function. However, its overproduction leads to nitrative stress by producing reactive nitrogen species. Here we measured NO content by Griess method and the localization of inducible and endothelial nitric oxide synthase (iNOS and eNOS) by immunohistochemistry in implanta- tion sites. Also we measured lipid peroxidation by TBA-RS, glutathion by Ellman's reaction and antioxidant enzymes by enzymatic kinetics in uterine homogenates. We found that the expression of both iNOS and eNOS and the NO content were increased with DHEA (p<0.001 for all) and restored to control levels with DHEA+M. Oxidative stress: DHEA in- creased lipid peroxidation (p<0.01) and glutathione (GSH, p<0.01). With DHEA+M lipid peroxidation was restored to control levels. The activities of the antioxidant enzymes superoxide dismutase and catalase were not modified. We con- clude that hyperandrogenization with DHEA enhances the NO system and lipid peroxidation in implantation sites of early pregnant mice and that M treatment prevents these effects.","PeriodicalId":88757,"journal":{"name":"The open reproductive science journal","volume":"37 1","pages":"14-22"},"PeriodicalIF":0.0,"publicationDate":"2013-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85031249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

No-CL Superstimulatory Protocol: Developing a New Superovulation Treatment Initiated in the Absence of Corpus Luteum (CL) and Compared with D1 and Traditional Superovulation in Cattle 无CL超刺激方案:在无黄体(CL)情况下开发一种新的超排卵治疗方法，并与D1和传统的牛超排卵进行比较

The open reproductive science journal Pub Date : 2012-02-10 DOI: 10.2174/1874255601204010004

T. El-Sherry, M. Matsui, K. Kida, A. Miyamoto, G. Megahed, S. Shehata, Y. Miyake

{"title":"No-CL Superstimulatory Protocol: Developing a New Superovulation Treatment Initiated in the Absence of Corpus Luteum (CL) and Compared with D1 and Traditional Superovulation in Cattle","authors":"T. El-Sherry, M. Matsui, K. Kida, A. Miyamoto, G. Megahed, S. Shehata, Y. Miyake","doi":"10.2174/1874255601204010004","DOIUrl":"https://doi.org/10.2174/1874255601204010004","url":null,"abstract":"The objective of this study was to investigate the effectiveness of superovulation under a complete absence of the Corpus luteum (CL) and compare it with D1 protocol and traditional superovulation in cattle. Animals were divided into three groups as following; 1- D1-protocol: Animals (n= 7) were leaved to get natural ovulation (D0), then received FSH for the next 4 days of the cycle. GnRH was given 12h after the last dose of FSH. 2- No-CL superovulation protocol: Animals (n=10) were synchronized and received PGF2α at D9 or D10 then classified to two subgroups (D9-sub-group and D10-sub-group). After 36 h, all follicles (≥ 5 mm) were aspirated (D0). 3- Control: Animals (n=3) were submitted to the conventional superovulation protocol. Blood samples were collected daily for 13 days. Progesterone (P 4 ) and Estradiol (E 2 ) in plasma were measured by Enzyme immune assay (EIA). The results showed that the number of growing follicles was significantly (P< 0.05) higher in both D9 and D10 subgroups in comparison to the D1 protocol (25.8 ± 4.3 and 20 ± 1.9 vs. 10.9 ± 1.9 respectively). While the number of ovulated follicle was higher in D9 sub-group than D10 sub-group, D1 protocol and control (13.8 ± 4.4 vs. 7.6 ± 3.5, 6.8 ± 1.5 and 9.7±0.9). In conclusion, the superovulation protocol with complete absent of the CL produced high number of growing follicles, decreased variability and considered as a promising superovulation protocol.","PeriodicalId":88757,"journal":{"name":"The open reproductive science journal","volume":"9 1","pages":"4-9"},"PeriodicalIF":0.0,"publicationDate":"2012-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78577814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HCG: Is it the Best Choice for Ovulation Triggering? 促性腺激素是促排卵的最佳选择吗?

The open reproductive science journal Pub Date : 2012-01-20 DOI: 10.2174/1874255601204010001

P. Humaidan, D. Bodri, E. Papanikolaou, S. Kol

引用次数: 4

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques 绵羊人工授精(AI)和胚胎移植(ET)的研究进展，特别是激素诱导宫颈扩张及其对动物生殖控制和外科技术的影响

The open reproductive science journal Pub Date : 2011-12-30 DOI: 10.2174/1874255601103010162

Ivanka B. R. Candappa, P. Bartlewski

{"title":"A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques","authors":"Ivanka B. R. Candappa, P. Bartlewski","doi":"10.2174/1874255601103010162","DOIUrl":"https://doi.org/10.2174/1874255601103010162","url":null,"abstract":"Artificial insemination (AI) and embryo transfer (ET) play an important role in the genetic improvement of sheep herds. This review primarily describes the methods that have recently been researched and/or adapted to circumvent the problematic anatomy of the ovine cervix, which prevents the widespread application of transcervical AI and ET in this species. A mechanism of cervical ripening at parturition and the endocrine influences on cervical tone are described as a backdrop to understanding the action of various pharmacological agents used to induce cervical dilation. Although several catheters have been designed to penetrate the ovine cervix, and a specific protocol has been developed for TCAI in sheep (Guelph Method), penetration rates and resulting pregnancy/lambing rates are still inconsistent and unpredictable. Hormonal dilation of the cervix may significantly improve the ease with which the ovine cervix can be traversed. Treatment with Cervidil ® , a dinoprostone-containing vaginal insert with a slow release mechanism, currently used to induce labor in women, could significantly improve cervical penetration in ewes without adverse effects on uterine contractility. This mode of drug delivery holds promise to result in the development of a safe method to induce cervical dilation for a broad range of transcervical reproductive manipulations in mammalian species including, but not limited to, AI, ET, embryo and oocyte recovery, treatment of ringwomb and transcervical surgical procedures. However, more studies are needed to evaluate the effects of hormonal methods on cervical morphology/pathomorphology, sperm/embryo transport and viability, and fertility in both cyclic and seasonally anovular ewes.","PeriodicalId":88757,"journal":{"name":"The open reproductive science journal","volume":"52 1","pages":"162-175"},"PeriodicalIF":0.0,"publicationDate":"2011-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84456807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36