Journal of angiogenesis research最新文献

{"title":"Platelet release of Vascular Endothelial Growth Factor (VEGF) in patients undergoing chemotherapy for breast cancer.","authors":"Cliona C Kirwan,&nbsp;Gerard J Byrne,&nbsp;Shant Kumar,&nbsp;Garry McDowell","doi":"10.1186/2040-2384-1-7","DOIUrl":"https://doi.org/10.1186/2040-2384-1-7","url":null,"abstract":"<p><strong>Background: </strong>Venous thromboembolism (VTE) following breast cancer chemotherapy is common. Chemotherapy-induced alterations in markers of haemostasis occur during chemotherapy. In this study we investigated the changes in serum and plasma VEGF, together with platelet release of VEGF and related these to the development of VTE at 3 months.</p><p><strong>Methods: </strong>Serum and plasma VEGF, together with platelet release of VEGF were measured prior to chemotherapy and at 24 hours; four-, eight days and three months following commencement of chemotherapy in early and advanced breast cancer patients and in age and sex matched controls. Duplex ultrasound imaging was performed after one month or if symptomatic.</p><p><strong>Results: </strong>Of 123 patients 9.8% developed VTE within three months. Serum and plasma VEGF were increased in advanced breast cancer as was platelet release of VEGF. Prior to chemotherapy a 100 microg/ml increase in serum VEGF was associated with a 40% increased risk of VTE, while a 10 microg/ml increase in plasma VEGF was associated with a 20% increased risk of VTE. Serum VEGF showed a different response to chemotherapy in those who developed VTE.</p><p><strong>Conclusion: </strong>A group of patients at risk of VTE could be identified, allowing targeted thromboprophylaxis. Whether or not the response in VEGF during chemotherapy has any angiogenic significance remains to be elucidated.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2009-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28599352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Welcome to Journal of Angiogenesis Research.","authors":"Mark Slevin,&nbsp;Yihai Cao,&nbsp;Jan Kitajewski","doi":"10.1186/2040-2384-1-1","DOIUrl":"https://doi.org/10.1186/2040-2384-1-1","url":null,"abstract":"<p><p>Angiogenesis is the growth of new blood vessels and is a key process which occurs during both physiological and pathological disease processes. Knowledge of the mechanisms through which this process is initiated and maintained will have a significant impact on the treatment of these diseases. Pathological angiogenesis occurs in major diseases such as cancer, diabetic retinopathies, age-related macular degeneration and atherosclerosis. In other diseases such as stroke and myocardial infarction, insufficient or improper angiogenesis results in tissue loss and ultimately higher morbidity and mortality.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2009-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DCE-MRI assessment of the effect of vandetanib on tumor vasculature in patients with advanced colorectal cancer and liver metastases: a randomized phase I study.","authors":"Klaus Mross,&nbsp;Ulrike Fasol,&nbsp;Annette Frost,&nbsp;Robin Benkelmann,&nbsp;Jan Kuhlmann,&nbsp;Martin Büchert,&nbsp;Clemens Unger,&nbsp;Hubert Blum,&nbsp;Jürgen Hennig,&nbsp;Tsveta P Milenkova,&nbsp;Jean Tessier,&nbsp;Annetta D Krebs,&nbsp;Anderson J Ryan,&nbsp;Richard Fischer","doi":"10.1186/2040-2384-1-5","DOIUrl":"10.1186/2040-2384-1-5","url":null,"abstract":"<p><strong>Background: </strong>Vandetanib is a once-daily oral inhibitor of VEGFR, EGFR and RET signaling pathways. In patients with advanced colorectal cancer and liver metastases, the effect of vandetanib on tumor vasculature was assessed using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI).</p><p><strong>Methods: </strong>Eligible patients received vandetanib 100 or 300 mg/day. DCE-MRI (iAUC(60 )and K(trans)) was used to quantify the primary endpoints of tumor perfusion and vascular permeability. An exploratory assessment of tumor oxygenation was performed using MRI/T2*. All MRI parameters were measured at baseline (twice) and on days 2, 8, 29 and 57.</p><p><strong>Results: </strong>Twenty-two patients received vandetanib (n = 10, 100 mg; n = 12, 300 mg). Baseline measurements of iAUC(60 )and K(trans )were reproducible, with low intrapatient coefficients of variation (11% and 24%, respectively). Estimates of mean % changes from baseline were -3.4% (100 mg) and -4.6% (300 mg) for iAUC(60), and -4.6% (100 mg) and -2.7% (300 mg) for K(trans); these changes were not significantly different between doses. The exploratory T2* measurement showed a significant increase at 300 mg versus 100 mg (P = 0.006). Both doses of vandetanib were generally well tolerated; common toxicities were fatigue, rash and diarrhea (majority CTC grade 1 or 2). The pharmacokinetic profile of vandetanib was similar to that observed previously. There were no RECIST-defined objective responses; five patients experienced stable disease >/=8 weeks.</p><p><strong>Conclusion: </strong>In this study in patients with advanced colorectal cancer, vandetanib did not modulate gadolinium uptake in tumor vasculature and tissue measured by the DCE-MRI parameters iAUC(60 )and K(trans).</p><p><strong>Trial registration: </strong>NCT00496509 (ClinicalTrials.gov); D4200C00050 (AstraZeneca).</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2009-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD105 positive neovessels are prevalent in early stage carotid lesions, and correlate with the grade in more advanced carotid and coronary plaques.","authors":"Ana Luque,&nbsp;Mark Slevin,&nbsp;Marta M Turu,&nbsp;Oriol Juan-Babot,&nbsp;Lina Badimon,&nbsp;Jerzy Krupinski","doi":"10.1186/2040-2384-1-6","DOIUrl":"https://doi.org/10.1186/2040-2384-1-6","url":null,"abstract":"<p><strong>Background: </strong>Previous studies have demonstrated that expression of CD105 is a sensitive marker and indicator of endothelial cell/microvessel activation and proliferation in aggressive solid tumour growth and atherosclerotic plaque lesions. Since intimal neovascularization contributes significantly to subsequent plaque instability, haemorrhage and rupture.</p><p><strong>Methods: </strong>We have used immunohistochemical analysis to investigate the expression of CD105-positive vessels in both large (carotid) and medium calibre (coronary and middle cerebral artery, MCAs) diseased vessels in an attempt to identify any correlation with plaque growth, stage and complication/type.</p><p><strong>Results: </strong>Here we show, that carotid arteries expressed intimal neovascularization associated with CD105-positive endothelial cells, concomitant with increased inflammation in early stage lesions, preatheroma (I-III) whilst they were not present in coronary plaques of the same grade. Some of these CD105-positive neovessels were immature, thin walled and without smooth muscle cell coverage making them more prone to haemorrhage and rupture. In high-grade lesions, neovessel proliferation was similar in both arterial types and significantly higher numbers of CD105-positive vasa vasorum were associated with plaque regions in coronary arteries. In contrast, although the MCAs exhibited expanded intimas and established plaques, there were very few CD105 positive neovessels.</p><p><strong>Conclusion: </strong>Our results show that CD105 is a useful marker of angiogenesis within adventitial and intimal vessels and suggest the existence of significant differences in the pathological development of atherosclerosis in separate vascular beds which may have important consequences when considering management and treatment of this disease.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2009-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"William Harvey and the discovery of the circulation of the blood.","authors":"Domenico Ribatti","doi":"10.1186/2040-2384-1-3","DOIUrl":"https://doi.org/10.1186/2040-2384-1-3","url":null,"abstract":"<p><p>This Commentary emphasizes the fundamental contribution of William Harvey to the discovery of the circulation of the blood and his scientific and experimental approach to this matter.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2009-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling the angiogenic switch in developing atherosclerotic plaques: possible targets for therapeutic intervention.","authors":"Mark Slevin,&nbsp;Jerzy Krupinski,&nbsp;Lina Badimon","doi":"10.1186/2040-2384-1-4","DOIUrl":"https://doi.org/10.1186/2040-2384-1-4","url":null,"abstract":"<p><p>Plaque angiogenesis may have an important role in the development of atherosclerosis. Vasa vasorum angiogenesis and medial infiltration provides nutrients to the developing and expanding intima and therefore, may prevent cellular death and contribute to plaque growth and stabilization in early lesions. However in more advanced plaques, inflammatory cell infiltration, and concomitant production of numerous pro-angiogenic cytokines may be responsible for induction of uncontrolled neointimal microvessel proliferation resulting in production of immature and fragile neovessels similar to that seen in tumour development. These could contribute to development of an unstable haemorrhagic rupture-prone environment. Increasing evidence has suggested that the expression of intimal neovessels is directly related to the stage of plaque development, the risk of plaque rupture, and subsequently, the presence of symptomatic disease, the timing of ischemic neurological events and myocardial/cerebral infarction. Despite this, there is conflicting evidence regarding the causal relationship between neovessel expression and plaque thrombosis with some in vivo experimental models suggesting the contrary and as yet, few direct mediators of angiogenesis have been identified and associated with plaque instability in vivo.In recent years, an increasing number of angiogenic therapeutic targets have been proposed in order to facilitate modulation of neovascularization and its consequences in diseases such as cancer and macular degeneration. A complete knowledge of the mechanisms responsible for initiation of adventitial vessel proliferation, their extension into the intimal regions and possible de-novo synthesis of neovessels following differentiation of bone-marrow-derived stem cells is required in order to contemplate potential single or combinational anti-angiogenic therapies. In this review, we will examine the importance of angiogenesis in complicated plaque development, describe the current knowledge of molecular mechanisms of its initiation and maintenance, and discuss possible future anti-angiogenic therapies to control plaque stability.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2009-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akt promotes endocardial-mesenchyme transition.","authors":"Kafi N Meadows,&nbsp;Seema Iyer,&nbsp;Mark V Stevens,&nbsp;Duanning Wang,&nbsp;Sharon Shechter,&nbsp;Carole Perruzzi,&nbsp;Todd D Camenisch,&nbsp;Laura E Benjamin","doi":"10.1186/2040-2384-1-2","DOIUrl":"https://doi.org/10.1186/2040-2384-1-2","url":null,"abstract":"<p><p>Endothelial to mesenchyme transition (EndMT) can be observed during the formation of endocardial cushions from the endocardium, the endothelial lining of the atrioventricular canal (AVC), of the developing heart at embryonic day 9.5 (E9.5). Many regulators of the process have been identified; however, the mechanisms driving the initial commitment decision of endothelial cells to EndMT have been difficult to separate from processes required for mesenchymal proliferation and migration. We have several lines of evidence that suggest a central role for Akt signaling in committing endothelial cells to enter EndMT. Akt1 mRNA was restricted to the endocardium of endocardial cushions while they were forming. The PI3K/Akt signaling pathway is necessary for mesenchyme outgrowth, as sprouting was inhibited in AVC explant cultures treated with the PI3K inhibitor LY294002. Furthermore, endothelial marker, VE-cadherin, was downregulated and mesenchyme markers, N-cadherin and Snail, were induced in response to expression of a constitutively active form of Akt1 (myrAkt1) in endothelial cells. Finally, we isolated the function of Akt1 signaling in the commitment to the transition using a transgenic model where myrAkt1 was pulsed only in endocardial cells and turned off after EndMT initiation. In this way, we determined that increased Akt signaling in the endocardium drives EndMT and discounted its other functions in cushion mesenchymal cells.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"1 ","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2009-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-1-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}